Abstract:
:An increase in light intensity induces a depolarization in retinal ON-bipolar cells via a reduced glutamate release from presynaptic photoreceptor cells. The underlying transduction cascade in the dendritic tips of ON-bipolar cells involves mGluR6 glutamate receptors signaling to TRPM1 proteins that are an indispensable part of the transduction channel. Several other proteins are recognized to participate in the transduction machinery. Deficiency in many of these leads to congenital stationary night blindness, because rod bipolar cells, a subgroup of ON-bipolar cells, constitute the main route for sensory information under scotopic conditions. Here, we review the current knowledge about TRPM1 ion channels and how their activity is regulated within the postsynaptic compartment of ON-bipolar cells. The functional properties of TRPM1 channels in the dendritic compartment are not well understood as they differ substantially from those of recombinant TRPM1 channels. Critical evaluation of possible explanations of these discrepancies indicates that some key components of this transduction pathway might still not be known. The continued exploration of this pathway will yield further clinically useful insights.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Schneider FM,Mohr F,Behrendt M,Oberwinkler Jdoi
10.1016/j.ejcb.2015.06.005subject
Has Abstractpub_date
2015-07-01 00:00:00pages
420-7issue
7-9eissn
0171-9335issn
1618-1298pii
S0171-9335(15)00064-3journal_volume
94pub_type
杂志文章,评审abstract::D-amino acid oxidase is expressed to a high level in the yeast Rhodotorula gracilis (0.3% of total cell protein) through induction by D-alanine in a defined growth medium. Monospecific polyclonal antibodies against pure enzyme were obtained. Western blot analysis showed that the enzyme is synthesized as the mature pol...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::Specific rabbit antisera were prepared against purified aggregation factor and its membrane-associated receptor, baseplate, derived from the marine sponge. Microciona prolifera. They were utilized in conjunction with fluorescent-labeled goat anti-rabbit IgG in an assay to demonstrate the surface localizations of both ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::Ovary gland cells of Aptenia cordifolia were exposed to 100 micrograms/ml cytochalasin B (cyt B) for 30 or 60 min during the phase of granulocrine polysaccharide secretion. The drug caused a congestion of Golgi vesicles around the dictyosomes, probably resulting from an inhibition of the vesicle migration towards the ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Embryonic stem (ES) cells are omnipotent; they can differentiate into every cell type of the body. The development of culture conditions that allow their differentiation has made it conceivable to produce large numbers of cells with lineage-specific characteristics in vitro. Here, we describe a method by which murine ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2007.07.001
更新日期:2007-12-01 00:00:00
abstract::This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80041-1
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abstract::To examine the transport of the single subunits of the glycoprotein complex gp80 (clusterin, apolipoprotein J), a marker protein for apical exocytosis and main secretory product of Madin-Darby canine kidney (MDCK) cells, several mutant cDNAs were constructed and expressed in the fibroblastic baby hamster kidney (BHK-2...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::Confluent bovine aortic endothelial cells (BAECs) formed a cobblestone-shaped cell monolayer when cultured on a collagen gel in a 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's-F12 supplemented with 5% newborn calf serum. Within a few days, however, they lost cell-cell attachment and became fibroblastic. ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::The spatial and temporal distribution as well as ultrastructural and biochemical characteristics of apoptotic and mitotic cells during human eye development were investigated in 14 human conceptuses of 4-9 postovulatory weeks, using electron and light microscopy. In the 5th developmental week, apoptotic and mitotic ce...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00328
更新日期:2003-08-01 00:00:00
abstract::The lectins Griffonia simplicifolia I and Lycopersicon esculentum were used to assess the presence of endothelium-specific glycoproteins in the microvasculature of the rat myocardium, diaphragm and superficial cerebral cortex. Organs fixed by intravascular perfusion were processed to obtain semithin (0.5 micron) and t...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::The trafficking of intracellular membranes requires the coordination of membrane-cytoskeletal interactions. Rab proteins are key players in the regulation of vesicular transport, while Rho family members control actin-dependent cell functions. We have previously identified a rho protein, rhoD, which is localized to th...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00173
更新日期:2001-06-01 00:00:00
abstract::Adoptive T cell therapy is the transfer of T cells to a patient in order to combat disease. This procedure is mainly being used but not limited to the treatment of viral infections and malignancies including virus-associated tumors. Depending on the clinical context, the T cell donor may be the same patient or another...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2011.04.001
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abstract::The importance of intact microtubules in the processing, storage and regulated secretion of von Willebrand factor (vWf) from Weibel-Palade bodies in endothelial cells was investigated. Human umbilical vein endothelial cells treated for 1 h with colchicine (10(-6) M) or nocodozole (10(-6) M) lost their organized microt...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::Na/K-ATPase of salt-stressed salt glands of the domestic duck (Anas platyrhynchos) was purified in membrane-bound form by incubation of the microsomal fraction with sodium dodecylsulphate and ATP followed by discontinuous sucrose gradient centrifugation. Gel electrophoresis of the purified plasma membrane preparation ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Sarcoplasmic reticulum fragments capable of accumulating calcium were isolated from rat skeletal muscle by differential and sucrose gradient centrifugation. The ability of these fragments to accumulate calcium was impaired by adding 2,2-bis-(p-chlorophenyl)-1,1,1-trichloroethane (DDT) to the assay medium at concentrat...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::Intracellular free Ca2+ and H+ were quantified in Chlamydomonas reinhardtii, using the fluorescent ion indicators Fura-2 and BCECF. We demonstrate that both indicators can be loaded into living cells as acetoxymethylesters. The esters were hydrolyzed intracellularly to genuine Fura-2 and BCECF capable of indicating ch...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(99)80099-5
更新日期:1999-03-01 00:00:00
abstract::Cytoskeletal components backing the brush border of the rat kidney proximal tubule cell were identified and compared with those of the well characterized intestinal brush border by immuneoverlay and immunocytochemistry. Antibodies reactive against the intestinal microvillus core components, villin and fimbrin, as well...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::Phagosomes with pathogenic mycobacteria, or with hydrophobic polystyrene beads of 1 micron in size, do not mature, but remain fusogenic towards early endosomes and do not fuse with lysosomes (de Chastellier et al. Eur. J. Cell Biol. 68, 167-182 (1995)). Both types of phagocytic particles display a close apposition to ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::The transport of the chitin synthase III, Chs3p, to the plasma membrane is temporally and spatially regulated. Chs3p is delivered to the plasma membrane at the beginning of the cell cycle, forming chitin rings, and at the end of the cell cycle, forming the primary septum. During the rest of the cell cycle, it is maint...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.10.006
更新日期:2006-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1078/0171-9335-00077
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abstract::NAC (nascent polypeptide associated complex) is a heterodimer consisting of an α and a β subunit. skNAC (skeletal and heart muscle-specific form of αNAC) is a variant of αNAC, which is induced in muscle differentiation and regeneration. We show here that skNAC expression is regulated by p38 MAPK, which plays a crucial...
journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
pub_type: 杂志文章
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abstract::The interaction between myelin basic protein (MBP) and G-actin was studied under nonpolymerizing conditions, i.e.,2mM HEPES, pH 7.5, 0.1 mM CaCl2 and 0.2 mM ATP. Fluorescence studies using pyrenyl-actin and the measurements of ATP hydrolysis rate show that MBP induces changes in the structure of the actin monomer simi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.03.010
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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