Abstract:
:Na/K-ATPase of salt-stressed salt glands of the domestic duck (Anas platyrhynchos) was purified in membrane-bound form by incubation of the microsomal fraction with sodium dodecylsulphate and ATP followed by discontinuous sucrose gradient centrifugation. Gel electrophoresis of the purified plasma membrane preparation substantially showed the two polypeptide subunits of the Na/K-ATPase both of which stained with the periodic acid-Schiff reagent. About 99% of the total ATPase activity was ouabain-inhibitable amounting to 1300 mumol Pi/(mg protein X h) of specific activity. The anion-stimulated, ouabain-insensitive ATPase increased parallel to the Na/K-ATPase up to the microsomal fraction until it totally vanished during SDS incubation. Electron microscopy of thin sections revealed that the purified fraction consisted of flat and cup-shaped triple-layered membrane fragments. Particles arranged into clusters and strands were visible as 3 to 5 nm surface particles in negatively stained suspensions and as 8 to 10 nm intramembraneous particles in freeze fracture replicas. The differential distribution of the intramembraneous particles on the fracture faces reflected the structural membrane asymmetry. Solubilization of Na/K-ATPase led to the disappearance of intramembraneous particles. Incorporation of the solubilized enzyme into phosphatidylcholine vesicles again showed 8 to 10 nm particles apparently orientated at random in the artificial membrane. Control liposomes prepared in the absence of solubilized enzyme were devoid of intramembraneous particles. These results clearly demonstrate that the avian salt gland Na/K-ATPase exists as 8 to 10 nm particles in both the purified plasma membrane and the artificial phospholipid membrane.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Gassner D,Komnick Hsubject
Has Abstractpub_date
1983-01-01 00:00:00pages
226-35issue
2eissn
0171-9335issn
1618-1298journal_volume
29pub_type
杂志文章abstract::Cells of Oocystis solitaria after treatment with inhibitors of a) microfibril orientation b) microfibril synthesis, and c) microfibril crystallization have been investigated with the freeze-fracturing technique. Changes in morphology of the E-face of the plasma membrane as a result of these treatments have been record...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::The nucleolar protein B-36 is an RNA-associated protein which has a number of properties in common with pre-mRNA-binding proteins (hnRNP proteins). Like the hnRNP proteins, B-36 appears to be evolutionarily conserved among various eukaryotes (protists and several animal species). The conservation of B-36 throughout th...
journal_title:European journal of cell biology
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abstract::A method using proteins covalently coupled to glass surfaces has been applied to studies on the differentiation of neuroblasts. Neuroblastoma cells from clones N 18 and NIE 115 adhere to surfaces coated with fibronectin or laminin and extend rapidly growth cone-containing neurites. Some lectin-coated surfaces are also...
journal_title:European journal of cell biology
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abstract::Cathepsin H is a unique member of the cysteine cathepsins that acts primarily as an aminopeptidase. Like other cysteine cathepsins, it is synthesized as an inactive precursor and activated by proteolytic removal of its propeptide. Here we demonstrate that, in human cells, the processing of the propeptide is an autocat...
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
abstract::Within eukaryotic cells tubulin generally exists in protein families composed of closely related isoforms generated either by differential gene transcription or by posttranscriptional and posttranslational mechanisms. In this study, immunological approaches were used to examine the contribution of posttranslational mo...
journal_title:European journal of cell biology
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abstract::This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the slime droplet enshe...
journal_title:European journal of cell biology
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更新日期:1984-05-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1981-12-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1990-10-01 00:00:00
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journal_title:European journal of cell biology
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doi:
更新日期:1994-02-01 00:00:00
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更新日期:1993-10-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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doi:
更新日期:1995-02-01 00:00:00
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
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更新日期:1984-11-01 00:00:00
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journal_title:European journal of cell biology
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abstract::Tight junctions (TJ) of the fascia occludens type can be rapidly assembled in the human colon adenocarcinoma cell line HT 29 under the influence of trypsin or ammonium sulfate. We have studied the influence of the metabolic inhibitors, dinitrophenol (DNP) and deoxyglucose (DG), on the induced formation of TJ in this c...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
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journal_title:European journal of cell biology
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doi:
更新日期:1986-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1994-12-01 00:00:00