Abstract:
:Cathepsin H is a unique member of the cysteine cathepsins that acts primarily as an aminopeptidase. Like other cysteine cathepsins, it is synthesized as an inactive precursor and activated by proteolytic removal of its propeptide. Here we demonstrate that, in human cells, the processing of the propeptide is an autocatalytic, multistep process proceeding from an inactive 41kDa pro-form, through a 30kDa intermediate form, to the 28kDa mature form. Tyr87P and Gly90P were identified as the two major endopeptidase cleavage sites, converting the 30kDa form into the mature 28kDa form. The level of processing differs significantly in different human cell lines. In monocyte-derived macrophages U937 and prostate cancer cells PC-3, the 28kDa form is predominant, whereas in osteoblasts HOS the processing from the 30kDa form to the 28kDa form is significantly lower. The aminopeptidase activity of the enzyme and its subcellular localization are independent of the product, however the 30kDa form was not secreted in HOS cells. The activity of the resulting cathepsin H in U937 cells was significantly lower than that in HOS cells, presumably due to the high levels of endogenous cysteine protease inhibitor cystatin F present specifically in this cell line. These results provide an insight into the dependence of human cathepsin H processing and regulation on cell type.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Rojnik M,Jevnikar ZR,Doljak B,Turk S,Zidar N,Kos Jdoi
10.1016/j.ejcb.2012.05.004subject
Has Abstractpub_date
2012-10-01 00:00:00pages
757-64issue
10eissn
0171-9335issn
1618-1298pii
S0171-9335(12)00086-6journal_volume
91pub_type
杂志文章abstract::Ovary gland cells of Aptenia cordifolia were exposed to 100 micrograms/ml cytochalasin B (cyt B) for 30 or 60 min during the phase of granulocrine polysaccharide secretion. The drug caused a congestion of Golgi vesicles around the dictyosomes, probably resulting from an inhibition of the vesicle migration towards the ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Temperatures around 20 degrees C are known to block degradation of endocytosed material by preventing its transport to lysosomes, accordingly reduced temperature has been widely used to define endosomes. Newer studies have revealed that the low temperature block is proximal to perinuclear late endosomes, but it is not...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/s0171-9335(98)80067-8
更新日期:1998-04-01 00:00:00
abstract::Hepatocytoma (HPCT) hybrid cells were obtained by fusion of cultured rat hepatocytes with Fao Reuber hepatoma cells H35 by polyethylene glycol treatment. Surviving cells were cloned in HAT (hypoxanthine-aminopterine-thymidine)/ouabain medium and propagated in cell lines over 80 passages. Morphological criteria were ch...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::The yeast dynamin Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains u...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.02.004
更新日期:2017-03-01 00:00:00
abstract::The macronuclear DNA synthesis in well-fed carnivorous forms of Tetrahymena paravorax has been investigated with autoradiographic experiments. The macrostome cell type of this ciliate is induced by stomatin and fed on living prey (T. pyriformis). The DNA synthesis is re-initiated when the macrostomes become active pre...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::Endogenous carbohydrate-binding sites were studied during rat cerebellar development on sections of fixed tissue using synthetic tools, biotinylated neoglycoproteins, in conjunction with subsequent avidinperoxidase staining. Neoglycoproteins were constructed by chemically coupling the histochemically pivotal carbohydr...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::The aim of this study was to evaluate the influence of peroxisome proliferator-activated receptor γ (PPARγ) on self-renewal of mouse embryonic stem cells (mESCs) in the presence and absence of leukemia inhibitory factor (LIF). We demonstrated that in the presence of LIF, the activation of PPARγ by Rosiglitazone led to...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2013.03.003
更新日期:2013-04-01 00:00:00
abstract::Leydig cells in developing fetal pig testis contained during the fetal regressive phase large accumulations of intermediate filaments. Before and after this period these filaments were arranged in a criss-cross fashion. In the pig as well as in the dog testis these filaments have been characterized as vimentin. Within...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::In this study, we investigated the effect of plant microtubule-associated proteins (MAPs) on microtubule nucleation and growth in vitro. Since it has recently been demonstrated that plant nuclear surface acts as a microtubule-organizing center (MTOC), we tested the effects of plant MAPs using a nucleus-mediated microt...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::The serine/arginine rich proteins (SR proteins) are members of a family of RNA binding proteins involved in regulating various features of RNA metabolism, including pre-mRNA constitutive and alternative splicing. In humans, a total of 12 SR splicing factors (SRSFs) namely SRSF1-SRSF12 have been reported. SRSF3, the sm...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2020.151099
更新日期:2020-08-01 00:00:00
abstract::The weak bases chloroquine, primaquine, NH4Cl and the ionophore monensin exert similar but not identical effects on sorting, transport and processing of cathepsin D in several human cell lines (fibroblasts, HepG2 cells, U937, monocytes). The drugs inhibit the segregation of newly synthesized cathepsin D from the secre...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::Transient receptor potential (TRP) cation channels represent a large and diverse family of ion channels that act as important transducers of sensory information. The Melastatin subfamily member TRPM7 has garnered much interest due to its functional kinase domain; a unique feature among ion channels. TRPM7 primarily co...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2014.07.001
更新日期:2014-10-01 00:00:00
abstract::Diaphanous-related formins (DRFs) drive the nucleation and elongation of linear actin filaments downstream of Rho GTPase signalling pathways. Dictyostelium formin C (ForC) resembles a DRF, except that it lacks a genuine formin homology domain 1 (FH1), raising the questions whether or not ForC can nucleate and elongate...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2013.07.001
更新日期:2013-06-01 00:00:00
abstract::Morphological explanations for the effects of cytochalasin B (CB) on the digestive vacuole system of Paramecium caudatum were sought using electron-microscopic studies. Cytochalasin B (0.3 mM) essentially stopped vacuole release without stopping vacuole growth. Acidosome fusion with the new vacuoles was inhibited and ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::Specific antibodies to a protein designated P1 (Mr approximately equal to 63,000), which is specifically altered in mutants resistant to the microtubule inhibitor podophyllotoxin, bind to mitochondria in cells of various vertebrate and invertebrate species (Eur. J. Cell Biol. 44, 278-285 (1987); Can. J. Biochem. Cell ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::We have analyzed the mode of uptake of human beta FcRII molecules expressed in BHK cells (clone 2/14). When challenged with aggregated human IgG (ahIgG), these cells bind the ligand at 4 degrees C and endocytose the IgG: receptor complexes rapidly upon warming to 37 degrees C, as seen by fluorescence microscopy with a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::1. Feeding weanling mice a diet containing 0.5% (w/w) cuprizone (biscyclohexanone oxaldihydrazone) induced megamitochondria of a different type of that previously described (Suzuki, K., Science 163, 81-82 (1969). 2. As in the normal controls, a single population of mitochondria with a narrow size distribution, was det...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::The evolutionary conserved transmembrane protein Crumbs (Crb) regulates morphogenesis of photoreceptor cells in the compound eye of Drosophila and prevents light-dependent retinal degeneration. Here we examine the role of Crb in the ocelli, the simple eyes of Drosophila. We show that Crb is expressed in ocellar photor...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2012.03.006
更新日期:2012-09-01 00:00:00
abstract::Initial integrin-mediated cell-matrix adhesions (focal complexes) appear underneath the lamellipodia, in the regions of the "fast" centripetal flow driven by actin polymerization. Once formed, these adhesions convert the flow behind them into a "slow", myosin II-driven mode. Some focal complexes then turn into elongat...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2005.11.001
更新日期:2006-04-01 00:00:00
abstract::In the present study, a systematic examination of the relative expression pattern of the nuclear retinoid receptors (RAR and RXR) in various liver cells was performed. Our data demonstrate that RXRalpha is the dominant receptor in all liver cells, and that RARbeta is also expressed at a high level in most cells. More ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80078-2
更新日期:1998-10-01 00:00:00
abstract::It has only recently been recognized that intermediate filaments (IFs) and their assembly intermediates are highly motile cytoskeletal components with cell-type- and isotype-specific characteristics. To elucidate the cell-type-independent contribution of actin filaments and microtubules to these motile properties, flu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2004.12.004
更新日期:2005-03-01 00:00:00
abstract::Cell adhesion to the extracellular matrix (ECM) is important in a variety of physiological and pathologic processes, including development, tumor invasion, and metastasis. Integrin-mediated attachment to ECM proteins has emerged to cue events primitively important for the transformed phenotype of human cancer cells. C...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2020.151083
更新日期:2020-05-01 00:00:00
abstract::Earlier studies have indicated that Jun/AP-1 activity is associated with, and probably required for apoptosis induction by DNA-damaging and stress-inducing agents in human myeloid cells. To investigate this possibility, we examined the capacity of continuous treatments with etoposide (10 microM) and camptothecin (0.4 ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70001-1
更新日期:2000-01-01 00:00:00
abstract::When human blood platelets spread on a substratum they increase their surface area as much as 4-fold. We investigated the mechanism of spreading by light microscopy and by scanning and transmission electron microscopy. Contact of a platelet with a glass surface induces formation of thin extensions which spread out ove...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
abstract::Pretreatment of Acanthamoeba castellanii (Neff strain) with sublytic concentrations of surface-active alkyltrimethylammonium salts (C12, C14, C16) enhanced ConA-mediated agglutination of the amoebae. Treatment with the surfactants alone did not affect the "spontaneous" agglutination of the amoebae. Electron microscopi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80041-1
更新日期:1998-01-01 00:00:00
abstract::Many hormones/factors influence the total body growth and development during embryonic life, and very few studies have been carried out to ascertain their effects on the individual organ system. In this study, the effect of exogenous insulin-like growth factor-I (IGF-I) on embryonic kidneys was investigated, and corre...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::Invadopodia are actin-rich, adhesive protrusions that extend into and remodel the extracellular matrix. They are associated with high levels of pericellular proteolysis and correlate with the invasive capacity of a variety of tumour cells. Invadopodia have, thus, been proposed to recapitulate key events of the metasta...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2010.04.007
更新日期:2011-02-01 00:00:00
abstract::In the present study, immunoelectron microscopy was used to characterize two types of coated vesicles at the trans Golgi reticulum (TGR) of rat early spermatids. Most of the coated vesicles and buds had a 18.4 +/- 0.5 nm thick electron-dense coat. The size of the vesicles without the coat was 102.6 +/- 3.6 nm. This co...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract::Proteins that are concentrated in specific compartments of the endomembrane system in order to exert their organelle-specific function must possess specific localization signals that prevent their transport to distal regions of the exocytic pathway. Some resident proteins of the endoplasmic reticulum (ER) that are kno...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70025-4
更新日期:2000-04-01 00:00:00