当前位置： SCI文献检索 > EUROPEAN JOURNAL OF CELL BIOLOGY期刊下所有文献 > Growth factor-induced morphological, physiological and molecular characteristics in cerebral endothelial cells.

Growth factor-induced morphological, physiological and molecular characteristics in cerebral endothelial cells.

Abstract：

:The capacity of vascular endothelial cells to modulate their phenotype in response to changes in environmental conditions is one of the most important characteristics of this cell type. Since different growth factors may play an important signalling role in this adaptive process we have investigated the effect of endothelial cell growth factor (ECGF) on morphological, physiological and molecular characteristics of cerebral endothelial cells (CECs). CECs grown in the presence of ECGF and its cofactor heparin exhibit an epithelial-like morphology (type I CECs). Upon removal of growth factors, CECs develop an elongated spindle-like shape (type II CECs) which is accompanied by the reorganization of actin filaments and the induction of alpha-actin expression. Since one of the most important functions of CECs is the creation of a selective diffusion barrier between the blood and the central nervous system (CNS), we have studied the expression of junction-related proteins in both cell types. We have found that removal of growth factors from endothelial cultures leads to the downregulation of cadherin and occludin protein levels. The loss of junctional proteins was accompanied by a significant increase in the migratory activity and an altered protease activity profile of the cells. TGF-beta1 suppressed endothelial migration in all experiments. Our data provide evidence to suggest that particular endothelial functions are largely controlled by the presence of growth factors. The differences in adhesiveness and migration may play a role in important physiological and pathological processes of endothelial cells such as vasculogenesis or tumor progression.

journal_name

Eur J Cell Biol

journal_title

European journal of cell biology

authors

Krizbai IA,Bauer H,Amberger A,Hennig B,Szabó H,Fuchs R,Bauer HC

doi

10.1078/0171-9335-00084

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

594-600

issue

9

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(04)70068-0

journal_volume

79

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF CELL BIOLOGY文献大全
  • Identification of tubulin-binding proteins of the chicken erythrocyte plasma membrane skeleton which colocalize with the microtubular marginal band.

    abstract：:The plasma membrane of nucleated erythrocytes contains a microtubular marginal band which appears to be associated with the plasma membrane skeleton. In this report, we identify two families of cytoskeletal proteins which may be involved in such an association. These proteins, of molecular mass 78 kDa and 48 kDa on SD...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Stetzkowski-Marden F,Deprette C,Cassoly R

    更新日期：1991-02-01 00:00:00

  • Gap junctions and rhombic particle arrays in the freeze-fractured mouse oocyte-follicle cell complex.

    abstract：:Intact follicles as well as defolliculated oocytes of the mouse were studied by freeze-fracture electron microscopy. In intact follicles the oocyte plasma membrane shows two prominent types of intra-membrane particle array:gap junctions and yet undescribed rhombic particle arrays. The gap junctions vary in size (from ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： van den Hoef MH,Bluemink JG,de Laat SW

    更新日期：1984-11-01 00:00:00

  • P446L-importin-beta inhibits nuclear envelope assembly by sequestering nuclear envelope assembly factors to the microtubules.

    abstract：:The P446L mutant Drosophila importin-beta (P446L-imp-beta) has been reported to prohibit--in dominant negative fashion--nuclear envelope (NE) assembly. Along elucidating the mode of action of P446L-imp-beta we studied in vitro NE assembly on Sepharose beads. While Drosophila embryo extracts support NE assembly over Se...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00324

    authors： Tirián L,Timinszky G,Szabad J

    更新日期：2003-07-01 00:00:00

  • A nuclear lamin of the nematode Caenorhabditis elegans with unusual structural features; cDNA cloning and gene organization.

    abstract：:This report describes the characterization of the nuclear lamin CeLam-1 of the nematode Caenorhabditis elegans by molecular analysis of the corresponding complete cDNA and gene sequences. The primary structure of CeLam-1, representing only the third non-vertebrate lamin sequence currently known, follows essentially th...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Riemer D,Dodemont H,Weber K

    更新日期：1993-12-01 00:00:00

  • Lymphocyte adhesion to endothelium derived from human lymphoid tissue.

    abstract：:The development of an efficient immune response depends on the capacity of antigen-specific lymphocytes to migrate into secondary lymphoid organs. The first step in the process of lymphocyte extravasation involves lymphocyte binding to the vascular endothelium. Although several adhesion receptors have been implicated ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Castro A,Bono MR,Simon V,Rosemblatt M

    更新日期：1996-05-01 00:00:00

  • Localization of glucocorticoid hormone receptors in mitochondria of human cells.

    abstract：:Glucocorticoid hormones regulate the transcription of nuclear genes by way of their cognate receptors. In addition, these hormones also modulate mitochondrial gene transcription by mechanisms which are as yet poorly understood. Using immunofluorescence labeling and confocal laser scanning microscopy we show that the g...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/S0171-9335(04)70033-3

    authors： Scheller K,Sekeris CE,Krohne G,Hock R,Hansen IA,Scheer U

    更新日期：2000-05-01 00:00:00

  • Fluorescent antibody localization of Microciona prolifera aggregation factor and its baseplate component.

    abstract：:Specific rabbit antisera were prepared against purified aggregation factor and its membrane-associated receptor, baseplate, derived from the marine sponge. Microciona prolifera. They were utilized in conjunction with fluorescent-labeled goat anti-rabbit IgG in an assay to demonstrate the surface localizations of both ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Kuhns WJ,Bramson S,Simpson TL,Burkart W,Jumblatt J,Burger MM

    更新日期：1980-12-01 00:00:00

  • Microtubule and centrosome distribution during sheep fertilization.

    abstract：:The distribution of microtubules and centrosomes was studied during sheep fertilization by electron and immunofluorescence microscopy. Tubulin and centrosomal material was identified with monoclonal anti-alpha-tubulin and MPM-2 antibodies, respectively. In ovulated eggs, microtubules were exclusively found in the meio...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Le Guen P,Crozet N

    更新日期：1989-04-01 00:00:00

  • Mutations in the v-mos gene abolish its ability to induce differentiation but not transformation.

    abstract：:The v-mos oncogene product has the ability to induce differentiation in human monocytic leukemia U937 cells, thereby arresting cell proliferation, and also exhibits transforming activity in mouse NIH3T3 cells. Mutation in the v-mos gene consisting of one or two amino acid substitutions in the putative ATP-binding doma...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Kurata N,Akiyama H,Hosoya H,Aoki H,Ishikawa K,Marunouchi T

    更新日期：1995-09-01 00:00:00

  • Na+/Ca2+ exchanger overexpression impairs calcium signaling in fibroblasts: inhibition of the [Ca2+] increase at the cell periphery and retardation of cell adhesion.

    abstract：:We examined the Ca2+ handling property and cell function of CCL39 fibroblasts highly overexpressing the cardiac isoform (NCX1) of Na+/ Ca2+ exchanger. In NCX1 transfectants in 146 mM Na+, ionomycin, alpha-thrombin or thapsigargin only produced a small transient increase in [Ca2+]i compared to the large increase seen i...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/S0171-9335(98)80038-1

    authors： Iwamoto T,Wakabayashi S,Imagawa T,Shigekawa M

    更新日期：1998-07-01 00:00:00

  • Effects of surface-active alkyltrimethylammonium salts on concanavalin A-mediated agglutination in Acanthamoeba castellanii.

    abstract：:Pretreatment of Acanthamoeba castellanii (Neff strain) with sublytic concentrations of surface-active alkyltrimethylammonium salts (C12, C14, C16) enhanced ConA-mediated agglutination of the amoebae. Treatment with the surfactants alone did not affect the "spontaneous" agglutination of the amoebae. Electron microscopi...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Paatero G,Isomaa B,Johansson M,Kiiskinen G

    更新日期：1981-08-01 00:00:00

  • Integrin-linked kinase and its partners: a modular platform regulating cell-matrix adhesion dynamics and cytoskeletal organization.

    abstract：:Integrin-linked kinase (ILK) represents a key component of integrin signaling complexes that functions in concert with multiple binding partners to transmit cues from the extracellular matrix environment to the actin cytoskeleton. Both gain- and loss-of-function approaches to study ILK have confirmed the essential rol...

    journal_title：European journal of cell biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ejcb.2005.09.002

    authors： Boulter E,Van Obberghen-Schilling E

    更新日期：2006-04-01 00:00:00

  • Oligomerization supports transport of a mutant secretory protein out of the endoplasmic reticulum.

    abstract：:To examine the transport of the single subunits of the glycoprotein complex gp80 (clusterin, apolipoprotein J), a marker protein for apical exocytosis and main secretory product of Madin-Darby canine kidney (MDCK) cells, several mutant cDNAs were constructed and expressed in the fibroblastic baby hamster kidney (BHK-2...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Losch A,Henkel K,Wagner M,Urban J,Koch-Brandt C

    更新日期：1996-02-01 00:00:00

  • Oligosaccharide trimming Man9-mannosidase is a resident ER protein and exhibits a more restricted and local distribution than glucosidase II.

    abstract：:The subcellular distribution of a recently described neutral trimming mannosidase, the Man9-mannosidase, has been investigated by immunoelectron microscopy in pig hepatocytes. This enzyme processes asparagine-linked N-acetylglucosamine2 mannose9 oligosaccharides by specifically cleaving three of the four alpha 1,2-lin...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Roth J,Brada D,Lackie PM,Schweden J,Bause E

    更新日期：1990-10-01 00:00:00

  • Rab3 dissociation and clathrin-mediated endocytosis, two key steps in the exo-endocytotic pathway of large dense-cored vesicles in primary cultures of superior cervical ganglia.

    abstract：:In this study we have used primary cultures of porcine superior cervical ganglia as a model system to study exo-endocytosis in sympathetic neurons. Pure neuronal cultures with a defined noradrenergic phenotype can be obtained when antimitotics are included in the culture medium, and the high yield from prenatal piglet...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Annaert WG,Partoens P,Slembrouck D,Bakker A,Jacob W,De Potter WP

    更新日期：1997-11-01 00:00:00

  • Epidermal growth factor receptor in chronic bile duct obstructed rats: implications for maintenance of hepatocellular mass.

    abstract：:Changes of the number and properties of the epidermal growth factor (EGF) receptor occur during liver regeneration and may be of importance in the maintenance of hepatocellular mass in liver cirrhosis. We therefore studied the changes in the number and distribution of EGF receptor in the development of liver cirrhosis...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Oguey D,Marti U,Reichen J

    更新日期：1992-10-01 00:00:00

  • Hydrocortisone is involved in regulating the proliferation and differentiation of mouse epidermal melanoblasts in serum-free culture in the presence of keratinocytes.

    abstract：:Mouse epidermal melanoblasts preferentially proliferated from disaggregated epidermal cell suspensions derived from newborn mouse skin in serum-free medium (MPM) supplemented with dibutyryl adenosine 3', 5' cyclic monophosphate (DBcAMP) and basic fibroblast growth factor (bFGF). After 12 days, almost all keratinocytes...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Hirobe T

    更新日期：1996-12-01 00:00:00

  • Lack of detectable kinetochores on some chromosomes in mouse x human hybrids.

    abstract：:When treated with an anti-kinetochore antibody present in the sera of scleroderma (var. CREST) patients, most chromosomes exhibit kinetochore dots at the position of the centromere. In this paper we report that some chromosomes in the mouse x human somatic cell hybrid fail to show these dots. In the early passages in ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Vig BK,Sternes K,Paweletz N

    更新日期：1991-12-01 00:00:00

  • Ultrastructural and anti-proteinase activity modifications of human alpha 2-macroglobulin induced by zinc and other divalent cations.

    abstract：:Native tetrameric alpha 2-macroglobulin molecules (alpha 2M) can be converted into a population of dimers by incubation with various divalent cations such as Zn, Cd, Mg, Cu, Ni, Co. This dissociation is completed within 30 min at 37 degrees C. These dimers have a characteristic shape and a size of about 16 X 8 nm, and...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Couture-Tosi E,Tapon-Bretaudière J,Pochon F,Barray M,Bros A,Delain E

    更新日期：1986-12-01 00:00:00

  • Endocytosis of human IgG:Fc receptor complexes by transfected BHK cells.

    abstract：:We have analyzed the mode of uptake of human beta FcRII molecules expressed in BHK cells (clone 2/14). When challenged with aggregated human IgG (ahIgG), these cells bind the ligand at 4 degrees C and endocytose the IgG: receptor complexes rapidly upon warming to 37 degrees C, as seen by fluorescence microscopy with a...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Höning S,Jockusch BM,Kreimer G,Veltel D,Robenek H,Engelhardt W,Frey J

    更新日期：1991-06-01 00:00:00

  • The effect of energy deprivation and hyperosmolarity upon tubular structures and electrophysiological parameters of muscle fibres.

    abstract：:The exhaustion of metabolically poisoned muscle fibres is accompanied by a one hundred-fold increase in potassium conductance. Under these conditions a normal membrane capacity was measured. Extracellular markers (lanthanum, ferritin) were traced in the transverse tubular system but not in the myoplasm or the sarcopla...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Fink R,Grocki K,Lüttgau HC

    更新日期：1980-04-01 00:00:00

  • Phosphorylation of microtubule-associated proteins MAP2a,b and MAP2c at Ser136 by proline-directed kinases in vivo and in vitro.

    abstract：:The microtubule-associated protein 2 (MAP2) and its juvenile splicing variant MAP2c contain a phosphorylation site at Ser136 which is part of a Ser-Pro motif. This site lies within the N-terminal region common to MAP2b and MAP2c. It has been mapped by site-directed mutagenesis of recombinant MAP2c and by a monoclonal ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Berling B,Wille H,Röll B,Mandelkow EM,Garner C,Mandelkow E

    更新日期：1994-06-01 00:00:00

  • Identification of two types of beta-COP vesicles in the Golgi complex of rat spermatids.

    abstract：:In the present study, immunoelectron microscopy was used to characterize two types of coated vesicles at the trans Golgi reticulum (TGR) of rat early spermatids. Most of the coated vesicles and buds had a 18.4 +/- 0.5 nm thick electron-dense coat. The size of the vesicles without the coat was 102.6 +/- 3.6 nm. This co...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Martínez-Menárguez JA,Geuze HJ,Ballesta J

    更新日期：1996-10-01 00:00:00

  • The F658G substitution in Saccharomyces cerevisiae cohesin Irr1/Scc3 is semi-dominant in the diploid and disturbs mitosis, meiosis and the cell cycle.

    abstract：:The sister chromatid cohesion complex of Saccharomyces cerevisiae includes chromosomal ATPases Smc1p and Smc3p, the kleisin Mcd1p/Scc1p, and Irr1p/Scc3p, the least studied component. We have created an irr1-1 mutation (F658G substitution) which is lethal in the haploid and semi-dominant in the heterozygous diploid irr...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2008.05.002

    authors： Cena A,Kozłowska E,Płochocka D,Grynberg M,Ishikawa T,Fronk J,Kurlandzka A

    更新日期：2008-10-01 00:00:00

  • Integrin alphavbeta3 binding to human alpha5-laminins facilitates FGF-2- and VEGF-induced proliferation of human ECV304 carcinoma cells.

    abstract：:Human ECV304 cells respond reproducibly by tube formation to complex basement membrane matrices. Laminins are major glycoproteins of basement membranes. We therefore studied the ability of ECV304 cells to attach to defined laminin isoforms and to fibronectin, and identified the involved laminin receptors. The cells bo...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00297

    authors： Genersch E,Ferletta M,Virtanen I,Haller H,Ekblom P

    更新日期：2003-03-01 00:00:00

  • Cdx1 promotes cellular growth of epithelial intestinal cells through induction of the secretory protein PAP I.

    abstract：:Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation. Cdx1and the Pancreatitis Associated Protein I (PAP I) are concomitantly expressed in the epithelial cells of the lower part of the intestinal crypts. Because Cdx1 is a transcription factor and PAP I, in ot...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00148

    authors： Moucadel V,Soubeyran P,Vasseur S,Dusetti NJ,Dagorn JC,Iovanna JL

    更新日期：2001-02-01 00:00:00

  • Variations in the ability of galactose and mannose-specific lectins to bind to cell wall surfaces during growth of the insect pathogenic fungus Paecilomyces farinosus.

    abstract：:The arrangement of carbohydrate molecules on surfaces of fungal cells may play an important role in nonself recognition of these microorganisms by potential invertebrate hosts. Changes in the ability of various galactose and mannose-specific lectins to bind to surface components on cell walls of the insect pathogen Pa...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Pendland JC,Boucias DG

    更新日期：1993-04-01 00:00:00

  • Membrane specializations in the ciliate Euplotes crassus at the site of interaction of the ampules with the plasma membrane.

    abstract：:In Euplotes crassus, the ampules which occur in groups that surround the cilia at their base are organelles which, unlike the extrusomes such as mucocysts and trichocysts, persist inside the cytoplasm and are not lost after discharge. By freeze-fracturing cells, we found membrane specializations denoted by the presenc...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Dallai R,Luporini P

    更新日期：1981-02-01 00:00:00

  • The apical localization of SGLT1 glucose transporter is determined by the short amino acid sequence in its N-terminal domain.

    abstract：:SGLT1, an isoform of Na+-dependent glucose cotransporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney, where it plays a pivotal role in the absorption and reabsorption of sugars, respectively. To search the domain responsible for the apical localization of ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00204

    authors： Suzuki T,Fujikura K,Koyama H,Matsuzaki T,Takahashi Y,Takata K

    更新日期：2001-12-01 00:00:00

  • The coincident distribution of calcium-rich membranes and kinetochore fibers at metaphase in living endosperm cells of Haemanthus.

    abstract：:We have coupled chlorotetracycline (CTC) fluorescence with polarized light microscopic observations of living mitotic endosperm cells of Haemanthus in order to compare the spatial distributions of Ca2+-rich endomembranes in the spindle with that of the kinetochore fibers during metaphase. In either control cells or in...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Wolniak SM,Hepler PK,Jackson WT

    更新日期：1981-08-01 00:00:00