Abstract：
:In the present study, immunoelectron microscopy was used to characterize two types of coated vesicles at the trans Golgi reticulum (TGR) of rat early spermatids. Most of the coated vesicles and buds had a 18.4 +/- 0.5 nm thick electron-dense coat. The size of the vesicles without the coat was 102.6 +/- 3.6 nm. This coat reacted with antibodies against clathrin. Immunolabeling for clathrin was almost restricted to the trans Golgi area (86% of the total labeling in the Golgi complex). In addition, we found a homogeneous population of small vesicles and buds bearing a approximately 10 nm thick coat which reacted with antibodies against beta-COP. beta-COP-immunoreactive vesicles were detected at the cis side (32%), lateral rims (27%) and trans face (40%) of the Golgi complex. The diameters of beta-COP-immunoreactive vesicles at the TGR and cis Golgi side were 53.9 +/- 1.3 and 42.1 +/- 1.1 nm, respectively. Cis Golgi elements were identified by using antibodies against markers of the Golgi intermediate compartment p 58, p 53 and Rab 2. Some beta-COP labeling was also found at the acrosomal membrane and associated buds. These results indicate that the TGR of early spermatids contains COP-coated vesicles which are different to those found at the cis Golgi side.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Martínez-Menárguez JA,Geuze HJ,Ballesta Jsubject
Has Abstractpub_date
1996-10-01 00:00:00pages
137-43issue
2eissn
0171-9335issn
1618-1298journal_volume
71pub_type
杂志文章abstract：:SNAREs are compartmentally specific membrane proteins required for intracellular membrane fusion. Homologues of the Saccharomyces cerevisiae protein Sec1p interact with, and are likely to be involved in regulation of, the syntaxin family of SNAREs. In yeast there are 7 functionally distinct syntaxins but only four cle...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/s0171-9335(98)80084-8
更新日期：1998-12-01 00:00:00
abstract：:As proposed by Hanahan and Weinberg (2000. Cell 100, 57-70) carcinogenesis requires crucial events such as (i) genomic instability, (ii) cell cycle deregulation, (iii) induction of a telomere length maintenance mechanism, and (iv) an angiogenic switch. By comparing the expression of p53, cyclin D1, p16, hTERT, and TSP...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2006.11.002
更新日期：2007-12-01 00:00:00
abstract：:Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The method for electron microscopy of the Ag-staining of NOR-specific proteins was adapted to tissue sections from solid organs. The distribution of the Ag-staining proteins in rat liver nucleoli after complete block of transcription caused by D-galactosamine was investigated. In control animals, the Ag-staining prote...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:Differences in the carbohydrate composition were found in the jellies and the cortex of the unfertilized Xenopus laevis egg using lectins and blood group antibodies. Blood group H trisaccharide was detected in the outer jelly and "A-like" oligosaccharide was found in the inner jelly. The blood group A trisaccharide wa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:To examine the transport of the single subunits of the glycoprotein complex gp80 (clusterin, apolipoprotein J), a marker protein for apical exocytosis and main secretory product of Madin-Darby canine kidney (MDCK) cells, several mutant cDNAs were constructed and expressed in the fibroblastic baby hamster kidney (BHK-2...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Cell adhesion to the extracellular matrix (ECM) is important in a variety of physiological and pathologic processes, including development, tumor invasion, and metastasis. Integrin-mediated attachment to ECM proteins has emerged to cue events primitively important for the transformed phenotype of human cancer cells. C...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2020.151083
更新日期：2020-05-01 00:00:00
abstract：:A novel cytochemical method for the in situ, ultrastructural localization of phospholipids in biological tissues is reported. The method is based on the enzyme-gold approach (M. Bendayan: J. Histochem. Cytochem. 29, 531, 1981). Phospholipase A2 from bee venom was adsorbed on colloidal gold particles (PLA2-gold) and ap...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:Lamin proteins are components of metazoan cell nuclei. During evolution, two classes of lamin proteins evolved, A- and B-type lamins. B-type lamins are expressed in nearly all cell types and in all developmental stages and are thought to be indispensable for cellular survival. In contrast, A-type lamins have a more re...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2008.06.001
更新日期：2008-11-01 00:00:00
abstract：:A zymogen granule fraction has been isolated from rat pancreas, and its purity has been assessed by biochemical and morphological criteria. Specific activities of two marker enzymes, amylase and chymotrypsin, are increased by 4.6 and 5.4-fold, respectively, as compared to the homogenate. The purified fraction is devoi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:Monoclonal antibodies were used to define cell surface antigens which are present on rat hepatocytes but are absent from hepatoma cells. One monoclonal antibody, referred to as Be 9.2, recognizes a major component of purified rat liver plasma membranes with a Mr of 110 000. This antigen (gp110) was not found in the tr...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:During Drosophila spermatogenesis transcriptional activity is mainly restricted to premeiotic stages. Translation during sperm morphogenesis, however, proceeds for several days, requiring a high stability for mRNAs translated postmeiotically. We studied expression of the Drosophila beta 2 tubulin gene, which is expres...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:MUC16/CA125 is over-expressed in human epithelial tumors including ovarian, breast and some other carcinomas. The purpose of this study is to investigate how cell surface MUC16 is functionally involved in tumor progression, with a special focus on the role of its cytoplasmic tail. Forced expression of C-terminal MUC16...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2013.10.005
更新日期：2013-08-01 00:00:00
abstract：:Mitotic PtK1 spindles were UV irradiated (285 nm) during metaphase and anaphase between the chromosomes and the pole. The irradiation, a rectangle measuring 1.4 x 5 microns parallel to the metaphase plate, severed between 90 and 100% of spindle microtubules (MTs) in the irradiated region. Changes in organization of MT...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:The development of an efficient immune response depends on the capacity of antigen-specific lymphocytes to migrate into secondary lymphoid organs. The first step in the process of lymphocyte extravasation involves lymphocyte binding to the vascular endothelium. Although several adhesion receptors have been implicated ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:The docking complex of peroxisomal matrix protein import is composed of PEX13 and PEX14 in all species analyzed so far, whereas only yeast appears to possess an additional component, PEX17. In this report we isolated PEX14 complexes of Neurospora crassa. Among the complex constituents, one protein designated as PEX33 ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.07.003
更新日期：2010-12-01 00:00:00
abstract：:The concept of pilot pseudopodia is reconsidered 30 years after its inauguration (Gerisch, G., Hülser, D., Malchow, D., Wick, U., 1975. Cell communication by periodic cyclic-AMP pulses. Phil. Trans. R. Soc. Lond. B 272, 181-192). The original hypothesis stated that protruding pseudopodia serve as dynamic sensory organ...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2006.05.002
更新日期：2006-09-01 00:00:00
abstract：:Taking advantage of monoclonal antibodies raised against chick hypertrophic chondrocytes (BD5, LA5, AD2) and osteoblasts (SB1, SB2, SB3, SB5), we have investigated expression of differentiation stage-specific antigens by further differentiating hypertrophic chondrocytes. Expression of all antigens was developmentally ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-06-01 00:00:00
abstract：:Cell cycle kinetics and energy metabolism of Ehrlich ascites tumor cells grown in glucose-deprived medium supplemented with uridine, were investigated in order to extend our knowledge about the significance of the metabolic conversion of glucose for cell cycle progression of these cells. Viability (dye exclusion test)...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:Zymogen granules (ZG) of rat pancreas have been isolated by the procedure of Pâquet et al. The granules lysed when exposed to alkaline pH (pH 8.2), and their membranes could be subfractionated by centrifugation on a sucrose gradient. Four discrete types of membranes corresponding to densities of 1.105, 1.085, 1.075, a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequen...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:The realization that a highly conserved family of membrane proteins are localized to transport vesicles and selectively interact with proteins anchored at appropriate target sites of membrane fusion inspired a simple and compelling explanation of how proteins might be transferred and segregated within the cell, the "S...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：
更新日期：1997-07-01 00:00:00
abstract：:The present study was undertaken because of the paucity of information on peroxisomes in molluscs and the increasing importance of these organisms as sensitive indicators of environmental pollution. Peroxisomes were identified by electron microscopy in all three main cell types of the digestive gland of the bivalve mo...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Podosomes and the immune synapse are integrin-mediated adhesive structures that share a common ring-like morphology. Both podosomes and immune synapses have a central core surrounded by a peripheral ring containing talin, vinculin and paxillin. Recent progress suggests significant parallels between the regulatory mech...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2008.01.011
更新日期：2008-09-01 00:00:00
abstract：:When resting human blood platelets are stimulated with thrombin, 50 to 60% of the G-actin polymerizes to F-actin within 60 seconds. The increase in F-actin is correlated with a corresponding decrease in the complex of G-actin with T beta 4. Within 5 seconds after stimulation, nucleation sites for pyrene actin polymeri...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:Diaphanous-related formins (DRFs) drive the nucleation and elongation of linear actin filaments downstream of Rho GTPase signalling pathways. Dictyostelium formin C (ForC) resembles a DRF, except that it lacks a genuine formin homology domain 1 (FH1), raising the questions whether or not ForC can nucleate and elongate...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2013.07.001
更新日期：2013-06-01 00:00:00
abstract：:The amoeba Dictyostelium discoideum produces and secretes multilamellar bodies (MLBs) mainly composed of amoebal membranes upon digestion of bacteria. After their secretion, the fate of these MLBs remains unknown. The aim of this study was to determine if protozoa can internalize and digest secreted D. discoideum MLBs...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2017.10.002
更新日期：2017-12-01 00:00:00
abstract：:A fodrin-like protein purified from porcine thyroid cells and characterized by its properties identical to those of pig brain spectrin (F. Regnouf et al., Eur. J. Biochem. 153, 313-319 (1985)) has been localized by immunofluorescence and electron immunocytochemistry in porcine and rat thyroid. Fodrin-like polypeptides...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:We have studied, at the electron microscope level, the reorganizations of nucleolar ultrastructure induced by actinomycin D (AMD) in different conditions of drug treatment associated with an inhibition of rRNA synthesis. We have analyzed in parallel the localizations of ribosomal genes, of their transcripts, of variou...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:The subcellular distribution of a recently described neutral trimming mannosidase, the Man9-mannosidase, has been investigated by immunoelectron microscopy in pig hepatocytes. This enzyme processes asparagine-linked N-acetylglucosamine2 mannose9 oligosaccharides by specifically cleaving three of the four alpha 1,2-lin...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
