Abstract：
:Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a regenerative stimulus following partial hepatectomy. Under conditions of normal growth, CHox-cad is expressed in the embryonic but not in the adult liver. In the regenerating adult liver, immunohistochemical analyses and in situ hybridization experiments revealed a varying CHox-cad expression pattern extending from 6 h to 11 days posthepatectomy. Early in regeneration, 6 h and 18 h after partial hepatectomy, CHox-cad expression is confined to hepatocytes present in the periphery of the lobuli and to putative hepatic progenitor cells in the portal spaces. At later stages, from 30 h posthepatectomy onwards, CHox-cad activity is detected predominantly in cells of the hepatic progenitor cell compartment, the portal spaces. By day 8, CHox-cad-expressing cells have occupied marginal positions in some portal spaces and appear to penetrate into the periportal parenchyma. With progressing regeneration CHox-cad activity gradually declines reaching low levels in cells of a few portal spaces by day 11 post hepatectomy. As expected for a putative transcription factor, CHox-cad protein is localized to the cell nucleus. Immunodetection of PCNA, a marker of cell proliferation, revealed a much more widespread distribution of mitotic cells as compared to CHox-cad-expressing cells. In the region of overlapping expression domains, coexpression of CHox-cad and PCNA in the same cells was rare in most instances. There is evidence to suggest that CHox-cad is involved in the differentiation of liver progenitor cells to mature hepatocytes. The similarity of the CHox-cad expression pattern in the early developing liver and in the adult regenerating liver may indicate the reactivation of a genetic program operative during early stages of liver morphogenesis.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Doll U,Niessing Jsubject
Has Abstractpub_date
1996-07-01 00:00:00pages
260-8issue
3eissn
0171-9335issn
1618-1298journal_volume
70pub_type
杂志文章abstract：:We have used plant root tips frozen under high pressure in conjunction with freeze-fracture electron microscopy a) to evaluate the quality of freezing of unfixed, non-cryoprotected tissues obtainable with this method, b) to examine the structure of cells frozen under high pressure, c) to evaluate the usefulness of hig...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:The murine recessive yellow (Mc1r(e)) is a loss-of-function mutation in the receptor for alpha-melanocyte-stimulating hormone, melanocortin receptor 1 (Mc1r) and produces yellow coats by inducing pheomelanin synthesis in hair follicular melanocytes. However, it is not known whether the Mc1r(e) mutation affects the pro...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2007.03.007
更新日期：2007-06-01 00:00:00
abstract：:Endothelial cell-specific molecules are potential targets for new therapeutic strategies in the control of inflammatory reactions, immune responses and neoangiogenesis. We describe the production and characterization of MEC 14.7, a monoclonal antibody directed to murine endothelial cells recognizing a glycosylated pro...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:The weak bases chloroquine, primaquine, NH4Cl and the ionophore monensin exert similar but not identical effects on sorting, transport and processing of cathepsin D in several human cell lines (fibroblasts, HepG2 cells, U937, monocytes). The drugs inhibit the segregation of newly synthesized cathepsin D from the secre...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:The subcellular distribution of a recently described neutral trimming mannosidase, the Man9-mannosidase, has been investigated by immunoelectron microscopy in pig hepatocytes. This enzyme processes asparagine-linked N-acetylglucosamine2 mannose9 oligosaccharides by specifically cleaving three of the four alpha 1,2-lin...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:During endotoxic shock, the liver exerts an endotoxin (lipopolysaccharide, LPS) clearance function with the participation of both sinusoidal (mainly Kupffer and endothelial cells) and parenchymal cells. In order to determine the specificity and diversity of response of each liver cell type, the effect of Escherichia c...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Fucoidan is a sulfated polysaccharide extracted from brown seaweeds. It has anticoagulant and antithrombotic properties and inhibits, as well as heparin, vascular smooth muscle cell growth. In this study, we investigated, in the presence of serum and human recombinant growth factors, the effects of fucoidan and hepari...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80094-0
更新日期：1998-12-01 00:00:00
abstract：:An increase in light intensity induces a depolarization in retinal ON-bipolar cells via a reduced glutamate release from presynaptic photoreceptor cells. The underlying transduction cascade in the dendritic tips of ON-bipolar cells involves mGluR6 glutamate receptors signaling to TRPM1 proteins that are an indispensab...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2015.06.005
更新日期：2015-07-01 00:00:00
abstract：:Rhodamine-conjugated phalloin, a derivative of phalloidin which binds to F-actin, was associated with three types of structures in uredospore germlings of the bean rust fungus, Uromyces phaseoli. The structures were filaments, peripheral plaques, and intranuclear inclusions. The filaments, located throughout the germ ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:A monoclonal antibody (ID5) raised against the synthetic tetradecapeptide corresponding to the C-terminal region of detyrosinated alpha-tubulin showed an unexpected cross-reactivity with beta-tubulin from pig brain tissue. The specificity and the minimal epitope requirements of ID5 were characterized by competitive en...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/s0171-9335(99)80003-x
更新日期：1999-01-01 00:00:00
abstract：:Appreciable amounts of glycosaminoglycans have been found by immunocytochemistry within mature elastin fibers of human dermis. On thin sections, elastin fibers showed antigenic sites for monoclonal antibodies recognizing the unsaturated units remaining after digestion of hyaluronic acid with Streptomyces hyaluronidase...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Filamentous networks were isolated from rat liver and studied by electron microscopy after negative staining with uranyl acetate. Highly purified preparations of networks were used to obtain material for immunological studies, but morphological studies were made more satisfactorily on preparations purified only by suc...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：:The P446L mutant Drosophila importin-beta (P446L-imp-beta) has been reported to prohibit--in dominant negative fashion--nuclear envelope (NE) assembly. Along elucidating the mode of action of P446L-imp-beta we studied in vitro NE assembly on Sepharose beads. While Drosophila embryo extracts support NE assembly over Se...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00324
更新日期：2003-07-01 00:00:00
abstract：:In the present study, a systematic examination of the relative expression pattern of the nuclear retinoid receptors (RAR and RXR) in various liver cells was performed. Our data demonstrate that RXRalpha is the dominant receptor in all liver cells, and that RARbeta is also expressed at a high level in most cells. More ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80078-2
更新日期：1998-10-01 00:00:00
abstract：:We have analyzed the mode of uptake of human beta FcRII molecules expressed in BHK cells (clone 2/14). When challenged with aggregated human IgG (ahIgG), these cells bind the ligand at 4 degrees C and endocytose the IgG: receptor complexes rapidly upon warming to 37 degrees C, as seen by fluorescence microscopy with a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Mitotic PtK1 spindles were UV irradiated (285 nm) during metaphase and anaphase between the chromosomes and the pole. The irradiation, a rectangle measuring 1.4 x 5 microns parallel to the metaphase plate, severed between 90 and 100% of spindle microtubules (MTs) in the irradiated region. Changes in organization of MT...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Centromeres support the assembly of the kinetochore on every chromosome and are therefore essential for the proper segregation of sister chromatids during cell division. Centromere identity is regulated epigenetically through the presence of the histone H3 variant CENP-A. CENP-A regulation and incorporation specifical...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2011.04.016
更新日期：2011-10-01 00:00:00
abstract：:Filensin is a lens-specific intermediate filament protein, expressed in the lens fiber cells but not the lens epithelium. Using antibodies to filensin and the other lens intermediate filament proteins, vimentin and CP49, the codistribution of filensin with CP49 and independence of this network from the vimentin networ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:Native tetrameric alpha 2-macroglobulin molecules (alpha 2M) can be converted into a population of dimers by incubation with various divalent cations such as Zn, Cd, Mg, Cu, Ni, Co. This dissociation is completed within 30 min at 37 degrees C. These dimers have a characteristic shape and a size of about 16 X 8 nm, and...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:The lectins Griffonia simplicifolia I and Lycopersicon esculentum were used to assess the presence of endothelium-specific glycoproteins in the microvasculature of the rat myocardium, diaphragm and superficial cerebral cortex. Organs fixed by intravascular perfusion were processed to obtain semithin (0.5 micron) and t...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:Proprotein Convertase 7 (PC7) is a Furin-like endoprotease that cleaves precursor proteins at basic amino acids. PC7 is concentrated in the trans-Golgi network (TGN) but it shuttles between the plasma membrane and the TGN depending on sequences in the cytoplasmic tail. A short region containing a three amino acids mot...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2017.03.010
更新日期：2017-08-01 00:00:00
abstract：:Direct conversion by overexpression of defined transcription factors (TFs) is a promising new method that can generate desired cell types from abundant, accessible cells. While previous studies have reported hepatic generation from fibroblasts, tremendous interest exists in the understanding of hepatic reprograming an...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2015.10.003
更新日期：2015-12-01 00:00:00
abstract：:The concept of pilot pseudopodia is reconsidered 30 years after its inauguration (Gerisch, G., Hülser, D., Malchow, D., Wick, U., 1975. Cell communication by periodic cyclic-AMP pulses. Phil. Trans. R. Soc. Lond. B 272, 181-192). The original hypothesis stated that protruding pseudopodia serve as dynamic sensory organ...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2006.05.002
更新日期：2006-09-01 00:00:00
abstract：:The muscle membrane of slow and fast fibers in cruralis and iliofibularis muscles and of intermediate fibers in submaxillaris muscle of the frog is studied in freeze-fracture replicas. A comparison of membrane folds, number, size and distribution of caveolae and of intramembrane particles (IMP) is given. In slow muscl...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:In the mouse genome, 20 connexin genes have been detected that code for proteins of high sequence identity in the two extracellular loops, especially six conserved cysteine residues. The mouse connexin23 (Cx23) gene (Gje1) differs from all other connexin genes in vertebrates, since it codes for a protein that contains...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2008.08.004
更新日期：2009-02-01 00:00:00
abstract：:The docking complex of peroxisomal matrix protein import is composed of PEX13 and PEX14 in all species analyzed so far, whereas only yeast appears to possess an additional component, PEX17. In this report we isolated PEX14 complexes of Neurospora crassa. Among the complex constituents, one protein designated as PEX33 ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.07.003
更新日期：2010-12-01 00:00:00
abstract：:The distribution of microtubules and centrosomes was studied during sheep fertilization by electron and immunofluorescence microscopy. Tubulin and centrosomal material was identified with monoclonal anti-alpha-tubulin and MPM-2 antibodies, respectively. In ovulated eggs, microtubules were exclusively found in the meio...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:A three-dimensional cytoskeleton, morphologically composed of intermediate filaments, desmosomes, and Z-disks, remains in bovine heart Purkinje fibres after extraction with Triton X-100 and low and high ionic strength solutions. Biochemically this cytoskeleton mainly consists of skeletin (Mr approximately 55 000) and ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:Activation of protein kinase C by phorbol esters triggers the remodelling of the actin cytoskeleton and the formation of podosomes in smooth muscle cells (SMCs). Regional control of actin dynamics at specialised microdomains results in a local reduction in contractile forces. The molecular basis for this local inhibit...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2005.08.001
更新日期：2006-04-01 00:00:00
