Abstract:
:In the mouse genome, 20 connexin genes have been detected that code for proteins of high sequence identity in the two extracellular loops, especially six conserved cysteine residues. The mouse connexin23 (Cx23) gene (Gje1) differs from all other connexin genes in vertebrates, since it codes for a protein that contains only 4 instead of 6 cysteine residues in the extracellular loops. Recently, two zebrafish connexin genes (Cx23a and Cx23b) have been identified, and a mouse mutant in the Gje1 gene has been described that exhibits a developmental defect in the lens. Here, we have compared the Cx23 gene in different mammalian species and found no transcripts in cDNA libraries of primates. Furthermore, all primate genomes analyzed contain stop codons in the Cx23 sequence, indicating inactivation of the orthologous primate GJE1 gene. No Cx23 mRNA was found in human eye. In order to analyze the properties of mouse Cx23 channels, we isolated HeLa cell clones stably expressing wild-type mCx23 or mCx23 fused to eGFP. Cells expressing Cx23-eGFP demonstrated its insertion in the plasma membrane but no punctate staining in contacting membranes characteristic for junctional plaques. In addition, we tested whether Cx23 forms functional gap junction channels electrophysiologically in cell pairs as well as by microinjection of neurobiotin and found that mouse Cx23 did not form gap junction channels in HeLa cells. However, there was a significant release of ATP from different Cx23 HeLa cell clones, even in the presence of normal culture medium with high calcium ion concentration, suggesting a hemichannel-based function of Cx23. Therefore, Cx23 seems to share functional properties with pannexin (hemi) channels rather than gap junction channels of other connexins.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Sonntag S,Söhl G,Dobrowolski R,Zhang J,Theis M,Winterhager E,Bukauskas FF,Willecke Kdoi
10.1016/j.ejcb.2008.08.004subject
Has Abstractpub_date
2009-02-01 00:00:00pages
65-77issue
2eissn
0171-9335issn
1618-1298pii
S0171-9335(08)00122-2journal_volume
88pub_type
杂志文章abstract::High-speed centrifugation supernatants from slime mould plasmodia show considerable activities to inhibit the polymerization of actin as revealed by viscosity measurements. By following increasing inhibitory activities an actin modulating protein (AM-protein) has been isolated and purified which affects the polymer st...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract::The interaction of daunomycin (DAU), an anthracyclinic antibiotic employed as antitumoral agent, with microtubules, has been investigated by cytochemical and morphological methods on a human melanoma cell line (H14). Results obtained indicated that DAU was able to modulate the microtubule reassembly in cells treated w...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::Rhodamine-conjugated phalloin, a derivative of phalloidin which binds to F-actin, was associated with three types of structures in uredospore germlings of the bean rust fungus, Uromyces phaseoli. The structures were filaments, peripheral plaques, and intranuclear inclusions. The filaments, located throughout the germ ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::We used botulinum neurotoxins (BoNT) to examine whether differences in the secretory activity of noradrenergic and adrenergic chromaffin cells are related to differences in the exocytotic machinery of these two types of bovine adrenal medulla cells. Cleavage of syntaxin and SNAP-25 by BoNT/C1 decreased in a dose-depen...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00128
更新日期:2000-12-01 00:00:00
abstract::The central feature of fluid and electrolyte secretion by salivary acinar cells is transepithelial Cl- movement as a driving force for the secretion. However, little is known about the membrane localization and regulation by agonists of various anion channels. To characterize the anion transport and fluid secretion, w...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70021-7
更新日期:2000-03-01 00:00:00
abstract::Exocytosis of synaptic vesicles (SV) results in the surface exposure of lumenal epitopes of SV proteins. We have recently described the use of antibodies directed against the lumenal N-terminus of synaptotagmin I (Sytlum-Abs) to morphologically monitor exo-endocytic recycling of SVs. We report here that a radioimmunoa...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::The exhaustion of metabolically poisoned muscle fibres is accompanied by a one hundred-fold increase in potassium conductance. Under these conditions a normal membrane capacity was measured. Extracellular markers (lanthanum, ferritin) were traced in the transverse tubular system but not in the myoplasm or the sarcopla...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::The monoclonal antibody MPM-12, raised by using partially purified extract of mitotic HeLa cells as the immunogen, preferentially stains the cytoplasm of mitotic cells by indirect immunofluorescence without exhibiting any species specificity. On immunoblots, MPM-12 recognizes three bands, of 155, 88, and 68 kDa, in mi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
abstract::The evolutionary conserved transmembrane protein Crumbs (Crb) regulates morphogenesis of photoreceptor cells in the compound eye of Drosophila and prevents light-dependent retinal degeneration. Here we examine the role of Crb in the ocelli, the simple eyes of Drosophila. We show that Crb is expressed in ocellar photor...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2012.03.006
更新日期:2012-09-01 00:00:00
abstract::Embryonic stem (ES) cells are omnipotent; they can differentiate into every cell type of the body. The development of culture conditions that allow their differentiation has made it conceivable to produce large numbers of cells with lineage-specific characteristics in vitro. Here, we describe a method by which murine ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2007.07.001
更新日期:2007-12-01 00:00:00
abstract::SNAREs are compartmentally specific membrane proteins required for intracellular membrane fusion. Homologues of the Saccharomyces cerevisiae protein Sec1p interact with, and are likely to be involved in regulation of, the syntaxin family of SNAREs. In yeast there are 7 functionally distinct syntaxins but only four cle...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/s0171-9335(98)80084-8
更新日期:1998-12-01 00:00:00
abstract::Perfused Chara cells capable of resuming ATP-dependent cytoplasmic streaming in low free Ca++ solutions have been examined by electron microscopy for myosin-like filaments. Filaments 44 nm in diameter and up to 3 micron in length have been found associated with the endoplasmic reticulum that along with mitochondria, m...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract::Programmed cell death (PCD) is the regulated removal of cells within an organism and plays a fundamental role in growth and development in nearly all eukaryotes. In animals, the model organism Caenorhabditis elegans (C. elegans) has aided in elucidating many of the pathways involved in the cell death process. Various ...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2012.02.002
更新日期:2012-08-01 00:00:00
abstract::Cell-matrix adhesions are essential for cell migration, tissue organization and differentiation, therefore playing central roles in embryonic development, remodeling and homeostasis of tissues and organs. Matrix adhesion-dependent signals cooperate with other pathways to regulate biological functions such as cell surv...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2008.02.012
更新日期:2008-09-01 00:00:00
abstract::We examined the secretion of three serum proteins, albumin (RSA), alpha 2 mu-globulin (alpha 2 mu G), and transferrin (Trf), in the isolated perfused liver. Within 4 h of perfusion, only 20 to 35% of previously synthesized proteins were secreted by the liver into the recirculating medium. Low temperature inhibited the...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::The weak bases chloroquine, primaquine, NH4Cl and the ionophore monensin exert similar but not identical effects on sorting, transport and processing of cathepsin D in several human cell lines (fibroblasts, HepG2 cells, U937, monocytes). The drugs inhibit the segregation of newly synthesized cathepsin D from the secre...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::Cell adhesion to the extracellular matrix (ECM) is important in a variety of physiological and pathologic processes, including development, tumor invasion, and metastasis. Integrin-mediated attachment to ECM proteins has emerged to cue events primitively important for the transformed phenotype of human cancer cells. C...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2020.151083
更新日期:2020-05-01 00:00:00
abstract::It was previously shown that avidin, a glycoprotein secreted in vivo by chicken oviduct, is produced by cultured transformed or damaged chicken embryo fibroblasts [27]. This report demonstrates synthesis and secretion of large amounts of avidin by macrophages isolated from chicken yolk sac. Avidin was secreted to the ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::Tubular aggregates are specific subcellular structures that appear in skeletal muscle fibres under different pathological conditions. The origin of the tubular aggregates is generally ascribed to proliferating membranes of sarcoplasmic reticulum. There are, however, histochemical indications for the presence of mitoch...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00230
更新日期:2002-02-01 00:00:00
abstract::During myocardial infarction, it is well known that the cytoplasm of cardiac myocytes becomes more acidic due to lactate accumulation. The resulting necrosis is believed to result, at least in part, from the leakage of lysosomal enzymes into the cytoplasm. In addition, it has previously been shown that cytoplasm acidi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Chondrocyte terminal differentiation is associated with cellular hypertrophy increased activity of plasma membrane alkaline phosphatase and the synthesis of collagen type X. The hypertrophic phenotype of cultured chondrocytes can be stimulated by ascorbic acid but the underlying mechanisms for this phenotypic change a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/s0171-9335(98)80023-x
更新日期:1998-06-01 00:00:00
abstract::Since various neuromuscular diseases are associated with abnormal glycosylation, it was of interest to determine whether this key post-translational modification is also altered in aged skeletal muscle. Lectins represent highly versatile carbohydrate-binding proteins that are routinely employed for the characterizatio...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2008.04.003
更新日期:2008-10-01 00:00:00
abstract::We have previously demonstrated the immunofluorescent localization of the tau and HMW MAPS on microtubules in vivo. When cells were treated with the non-ioni detergent triton before fixation, however treated with the non-ionic detergent triton before fixation, however, the immunofluorescent staining of microtubules wi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Changes in the surface morphology of regenerating rabbit, rat and frog corneal endothelial cells in vivo have been investigated by scanning electron microscopy. In adult tissue these cells do not normally divide unless given a stimulus, such as injury. Surfaces of quiescent rabbit and rat cells are devoid of microvill...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract::The nucleolar protein B-36 is an RNA-associated protein which has a number of properties in common with pre-mRNA-binding proteins (hnRNP proteins). Like the hnRNP proteins, B-36 appears to be evolutionarily conserved among various eukaryotes (protists and several animal species). The conservation of B-36 throughout th...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::The AtT20 pituitary cell is the one that was originally used to define the pathways taken by secretory proteins in mammalian cells. It possesses two secretory pathways, the constitutive for immediate secretion and the regulated for accumulation and release under hormonal stimulation. It is in the regulated pathway, mo...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00365
更新日期:2004-04-01 00:00:00
abstract::Procaine-treated eggs can be penetrated by more than one spermatozoon. Supernumerary male pronuclei can fuse with the female one giving raise to multipolar spindles or remain isolated within the egg's cytoplasm forming their own spindle. In all types of multiple mitotic figures (asters and spindles) the distribution o...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::The receptor-mediated endocytosis and intracellular processing of transferrin and mannose receptor ligands were investigated in bone marrow-derived macrophages, fibroblasts and reticulocytes. Mannosylated bovine serum albumin (BSA) conjugated to colloidal gold (Au-man-BSA) or colloidal gold-transferrin (AuTf) were use...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::Translationally controlled tumor protein (Tpt1/TCTP) is a multi-functional cytosolic protein whose cellular levels are finely tuned. TCTP regulates protein behavior by favoring stabilization of protein partners or on the contrary by promoting degradation of others. TCTP has been shown to be transcriptionally and trans...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2016.12.002
更新日期:2017-03-01 00:00:00
abstract::Microbodies containing bipyramidal crystalline nucleoid inclusions occur within every cortical cell in roots of Yucca torreyi. Reaction product deposition attributable to catalase, glycolate oxidase, and urate oxidase activities are cytochemically localized to Yucca root microbodies and classifies them as unspecialize...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00