HSV infection of polarized epithelial cells on filter supports: implications for transport assays and protein localization.

Abstract:

:Epithelial cell lines can be grown on filter supports and form polarized monolayers with distinct basolateral and apical plasma membrane domains. This property has been extensively used in cell biology to investigate epithelial cell function. To date, a major limitation of this approach has been the difficulty of obtaining transient gene expression in polarized epithelia. Here we present an approach to overcome this problem using gene transfer into polarized epithelial cells grown on filters using a herpes virus-based vector. Recombinant genes are inserted into a defective HSV-1 plasmid and packaged with a replication-incompetent HSV-1 helper virus into virus particles which are used to infect the polarized epithelial cells grown on filters. The transepithelial resistance of the cells is not affected by the addition of virus, and there are no detectable cytopathic effects.

journal_name

Eur J Cell Biol

authors

Murphy C,Zacchi P,Parton RG,Zerial M,Lim F

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

278-81

issue

3

eissn

0171-9335

issn

1618-1298

journal_volume

72

pub_type

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