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Localization of ribophorin II to the endoplasmic reticulum involves both its transmembrane and cytoplasmic domains.

Abstract：

:Proteins that are concentrated in specific compartments of the endomembrane system in order to exert their organelle-specific function must possess specific localization signals that prevent their transport to distal regions of the exocytic pathway. Some resident proteins of the endoplasmic reticulum (ER) that are known to escape with low efficiency from this organelle to a post ER compartment are recognized by a recycling receptor and brought back to their site of residence. Other ER proteins, however, appear to be retained in the ER by mechanisms that operate in the organelle itself. The mammalian oligosaccharyltransferase (OST) is a protein complex that effects the cotranslational N-glycosylation of newly synthesized polypeptides, and is composed of at least four rough ER-specific membrane proteins: ribophorins I and II (RI and RII), OST48, and Dadl. The mechanism(s) by which the subunits of this complex are retained in the ER are not well understood. In an effort to identify the domains within RII responsible for its ER localization we have studied the fate of chimeric proteins in which one or more RII domains were replaced by the corresponding ones of the Tac antigen, the latter being a well characterized plasma membrane protein that lacks intrinsic ER retention signals and serves to provide a neutral framework for the identification of retention signals in other proteins. We found that the luminal domain of RII by itself does not contain retention information, while the cytoplasmic and transmembrane domains contain independent ER localization signals. We also show that the retention function of the transmembrane domain is strengthened by the presence of a flanking luminal region consisting of 15 amino acids.

journal_name

Eur J Cell Biol

journal_title

European journal of cell biology

authors

Fu J,Pirozzi G,Sanjay A,Levy R,Chen Y,De Lemos-Chiarandini C,Sabatini D,Kreibich G

doi

10.1078/S0171-9335(04)70025-4

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

219-28

issue

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(04)70025-4

journal_volume

pub_type

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Localization of ribophorin II to the endoplasmic reticulum involves both its transmembrane and cytoplasmic domains.