Abstract:
:Three-dimensional collagen lattice cultures of fibroblasts mimic the in vivo situation better than monolayer cultures. Here, skin fibroblasts from scleroderma patients and healthy controls were cultivated in collagen lattices, and the effects of recombinant human gamma-interferon (IFN-gamma) on these cultures investigated. IFN-gamma inhibited collagen lattice retraction in a dose-dependent way at concentrations ranging from 10 to 10,000 U/ml. This effect was independent of any alteration to the cell proliferation within the lattices. The inhibition was of the same order of magnitude in normal and pathological fibroblasts. The synthesis of collagen and non-collagen proteins, particularly fibronectin, was increased in scleroderma cultures. It was inhibited in both normal and scleroderma fibroblasts by IFN-gamma, with a maximal effect at the concentration 1000 U/ml, but the inhibition of protein synthesis was far more intense in scleroderma than in normal cells. In situ hybridization, Northern blot and dot blot analyses showed that mRNA coding for pro alpha 1(I) collagen was decreased in IFN-gamma-treated cells, indicating an effect at the pretranslational level. IFN-gamma also inhibited glycosaminoglycan synthesis, but in scleroderma cells only. This study shows that IFN-gamma regulates cell behavior in three-dimensional collagen matrices: (i) it decreases protein and specifically glycosaminoglycan synthesis in scleroderma fibroblasts, (ii) it modulates the interactions between cells and matrix that lead to the retraction of the lattice. Whereas collagen synthesis is largely decreased in lattice cultures like in vivo, it remains increased in the case of scleroderma compared to normal fibroblasts and may be down-regulated by IFN-gamma. Similar conclusions may be drawn for fibronectin and glycosaminoglycans. The inhibitory effect of IFN-gamma on the retraction capacity of fibroblasts and on their ability to synthesize increased amounts of extracellular matrix macromolecules may be of potential interest for therapeutic use of IFN-gamma in scleroderma patients.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Gillery P,Serpier H,Polette M,Bellon G,Clavel C,Wegrowski Y,Birembaut P,Kalis B,Cariou R,Maquart FXsubject
Has Abstractpub_date
1992-04-01 00:00:00pages
244-53issue
2eissn
0171-9335issn
1618-1298journal_volume
57pub_type
杂志文章abstract::The exhaustion of metabolically poisoned muscle fibres is accompanied by a one hundred-fold increase in potassium conductance. Under these conditions a normal membrane capacity was measured. Extracellular markers (lanthanum, ferritin) were traced in the transverse tubular system but not in the myoplasm or the sarcopla...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::Evidence for the presence of a Cdc2-like protein in Physarum polycephalum has been obtained using a peptide antibody directed against a highly conserved amino acid sequence near the N-terminal end of Cdc2, Cdc28 and Cdc2HS. The antibody detected a 34 kDa cytoplasmic protein, similar in apparent size to Cdc2 in yeast a...
journal_title:European journal of cell biology
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doi:
更新日期:1989-02-01 00:00:00
abstract::Mutations in the LMNA gene, which encodes nuclear lamins A and C by alternative splicing, can give rise to Emery-Dreifuss muscular dystrophy. The mechanism by which lamins A and C separately contribute to this molecular phenotype is unknown. To address this question we examined ten LMNA mutations exogenously expressed...
journal_title:European journal of cell biology
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abstract::Isolated microtubule protein from axopodia of the heliozoan Echinosphaerium nucleofilum, consisting of two major bands on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), has been compared to axonemal and cytoplasmic tubulins from both animal and non-animal sources. The upper E. nucleofilum protein band migrated fas...
journal_title:European journal of cell biology
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更新日期:1983-07-01 00:00:00
abstract::Storage and release of proteins from granules forms the basis of cellular functions as diverse as cell mediated cytotoxicity, neuronal communication, activation of muscle fibres, and release of hormones or digestive enzymes from endocrine and exocrine glands, such as the pancreas. Serglycin is the major intracellular ...
journal_title:European journal of cell biology
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doi:10.1016/j.ejcb.2009.03.002
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abstract::Alternative splicing in the extracellular domain is a characteristic feature of members of the fibroblast growth factor receptor (FGFR) family. This splicing event generates receptor variants, which differ in their ligand binding specificities. A poorly characterized splice variant is FGFR1-IIIb, recently found to be ...
journal_title:European journal of cell biology
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doi:10.1078/0171-9335-00355
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abstract::Oocyte-thymocyte mouse cell hybrids were produced using polyethylene glycol (PEG) and examined at the ultrastructural level. Fusion was accomplished either before or after activation of metaphase II oocytes. In both experimental variants thymocyte nuclei undergo remodelling which comprises the following sequence of ev...
journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1986-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70025-4
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abstract::Integrin-linked kinase (ILK) represents a key component of integrin signaling complexes that functions in concert with multiple binding partners to transmit cues from the extracellular matrix environment to the actin cytoskeleton. Both gain- and loss-of-function approaches to study ILK have confirmed the essential rol...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2005.09.002
更新日期:2006-04-01 00:00:00
abstract::This report describes the characterization of the nuclear lamin CeLam-1 of the nematode Caenorhabditis elegans by molecular analysis of the corresponding complete cDNA and gene sequences. The primary structure of CeLam-1, representing only the third non-vertebrate lamin sequence currently known, follows essentially th...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Human HeLa or SkHep1 cells, defective in intercellular communication through gap junctions, were transfected with coding sequences of murine connexin40 (Cx40) and -43. The transfected cells were restored in gap junctional coupling as shown by 100-fold increased electrical conductance. When studied by the double whole-...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Proprotein Convertase 7 (PC7) is a Furin-like endoprotease that cleaves precursor proteins at basic amino acids. PC7 is concentrated in the trans-Golgi network (TGN) but it shuttles between the plasma membrane and the TGN depending on sequences in the cytoplasmic tail. A short region containing a three amino acids mot...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.03.010
更新日期:2017-08-01 00:00:00
abstract::HeLa cells in mitosis are fixed by KMnO4 to obtain a good preservation of membranes. Inside and around the mitotic apparatus a vesicular, a tubular and a cisternal type of membranes are demonstrated. Small spherical or ellipsoidal vesicles are distributed at random in the spindle area. Zones around the poles and in th...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::In the oil containing spores of Bryum capillare, lipid breakdown occurs within three days after sowing. The periods of activity of acid, neutral and alkaline lipases are coincident. They start at the first day after sowing the spores. After 3 days of germination, when storage fats forming large globules are mobilised ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract::Actin and tubulins of Phytophthora infestans germlings were detected with monoclonal antibodies on Western blots of crude extracts separated by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The Mr of actin was approximately 43,000, whereas alpha- and beta-tubulin, which migrated...
journal_title:European journal of cell biology
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doi:
更新日期:1990-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
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journal_title:European journal of cell biology
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doi:10.1016/j.ejcb.2004.12.030
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journal_title:European journal of cell biology
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doi:
更新日期:1986-04-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
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doi:
更新日期:1993-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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