Abstract:
:Cancer/testis antigens (CTAs) are characterized by their restricted expression pattern. In normal individuals their expression is largely restricted to the testis. In the case of cancer patients, CTA expression has also been frequently observed in the tumoral cells. CTAs are considered to be promising targets for immunotherapy. However, almost nothing is known about the properties defined by the vast majority of CTAs. Here, we have investigated the expression pattern and localization of the CTA CAGE-1 during mouse spermatogenesis. We show that protein CAGE-1 is 849 amino acids long. Analysis of the first spermatogenic wave of pubertal mice by RT-PCR and immunoblotting showed that CAGE-1 is predominantly expressed during postmeiotic stages. CAGE-1 localizes to the acrosomal matrix and acrosomal granule, as demonstrated by immunocytochemistry at the light and electron microscopic level. Taken together, our results allowed to define protein CAGE-1 as a novel component of the acrosome of mammalian spermatids and spermatozoa.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Alsheimer M,Drewes T,Schütz W,Benavente Rdoi
10.1016/j.ejcb.2004.11.003subject
Has Abstractpub_date
2005-03-01 00:00:00pages
445-52issue
2-3eissn
0171-9335issn
1618-1298pii
S0171-9335(04)00039-1journal_volume
84pub_type
杂志文章abstract::The importance of intact microtubules in the processing, storage and regulated secretion of von Willebrand factor (vWf) from Weibel-Palade bodies in endothelial cells was investigated. Human umbilical vein endothelial cells treated for 1 h with colchicine (10(-6) M) or nocodozole (10(-6) M) lost their organized microt...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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pub_type: 杂志文章,评审
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.06.001
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abstract::Mouse epidermal melanoblasts preferentially proliferated from disaggregated epidermal cell suspensions derived from newborn mouse skin in serum-free medium (MPM) supplemented with dibutyryl adenosine 3', 5' cyclic monophosphate (DBcAMP) and basic fibroblast growth factor (bFGF). After 12 days, almost all keratinocytes...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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