当前位置： SCI文献检索 > EUROPEAN JOURNAL OF CELL BIOLOGY期刊下所有文献 > Sealing the live part of the skin: the integrated meshwork of desmosomes, tight junctions and curvilinear ridge structures in the cells of the uppermost granular layer of the human epidermis.

Sealing the live part of the skin: the integrated meshwork of desmosomes, tight junctions and curvilinear ridge structures in the cells of the uppermost granular layer of the human epidermis.

Abstract：

:In the literature the question of whether a system structurally and functionally related to the barrier function of the tight junctions (TJs) of polarized epithelia exists in the epidermis has been and still is controversially discussed. We have systematically addressed this question in a study of the granular layer of fetal and adult human epidermis, combining different light and electron microscopic methods. We show that the lateral membranes of the cells of the stratum granulosum are connected by an extended subapical complex system integrating desmosomes and TJ structures identified as sites of close membrane-membrane contact and as regions of membrane-to-membrane apposition that in immunoelectron microscopy are positive for TJ marker proteins, notably occludin, indicative of an extended, probably continuous TJ barrier. In addition, we have noted in freeze-fractures of the apical membrane attaching this layer to the basalmost membrane of the stratum corneum an extended system integrating desmosomes with intramembraneous ridge configurations that appear as strands, circles, lariats or complex meshworks showing numerous continuities with the desmosomes. In some regions this system interconnecting desmosomes with curvilinear ridge structures occupies the major part of the plasma membrane. The molecular organizations and possible functional contributions of both structural systems positioned at the border between the living portion of the epidermis and the corneal layer are discussed, in particular in relation to the formation of a stable association between the two layers and of a barrier to the paracellular flow of molecules and particles. It is also discussed whether similar structures occur in other keratinizing stratified squamous epithelia, in squamous metaplasias and in tumors derived from such tissues.

journal_name

Eur J Cell Biol

journal_title

European journal of cell biology

authors

Schlüter H,Wepf R,Moll I,Franke WW

doi

10.1078/0171-9335-00434

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

655-65

issue

11-12

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(04)70375-1

journal_volume

83

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF CELL BIOLOGY文献大全
  • Oligomerization supports transport of a mutant secretory protein out of the endoplasmic reticulum.

    abstract：:To examine the transport of the single subunits of the glycoprotein complex gp80 (clusterin, apolipoprotein J), a marker protein for apical exocytosis and main secretory product of Madin-Darby canine kidney (MDCK) cells, several mutant cDNAs were constructed and expressed in the fibroblastic baby hamster kidney (BHK-2...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Losch A,Henkel K,Wagner M,Urban J,Koch-Brandt C

    更新日期：1996-02-01 00:00:00

  • Mutations in vimentin disrupt the cytoskeleton in fibroblasts and delay execution of apoptosis.

    abstract：:To get new insights into the function of the intermediate filament (IF) protein vimentin in cell physiology, we generated two mutant cDNAs, one with a point mutation in the consensus motif in coil1A (R113C) and one with the complete deletion of coil 2B of the rod domain. In keratins and glia filament protein (GFAP), a...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2005.09.019

    authors： Schietke R,Bröhl D,Wedig T,Mücke N,Herrmann H,Magin TM

    更新日期：2006-01-01 00:00:00

  • Membrane specializations in the ciliate Euplotes crassus at the site of interaction of the ampules with the plasma membrane.

    abstract：:In Euplotes crassus, the ampules which occur in groups that surround the cilia at their base are organelles which, unlike the extrusomes such as mucocysts and trichocysts, persist inside the cytoplasm and are not lost after discharge. By freeze-fracturing cells, we found membrane specializations denoted by the presenc...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Dallai R,Luporini P

    更新日期：1981-02-01 00:00:00

  • An inhibitor of the chymotrypsin-like activity of the multicatalytic proteinase complex (20S proteasome) induces arrest in G2-phase and metaphase in HeLa cells.

    abstract：:HeLa cells were treated with different concentrations of an inhibitor of the proteasome chymotrypsin-like activity, the peptidyl aldehyde N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (PSI). A detailed analysis, which included flow cytometry, cell counting and morphological assessment, was performed. PSI treatme...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Wójcik C,Schroeter D,Stoehr M,Wilk S,Paweletz N

    更新日期：1996-06-01 00:00:00

  • Detection of binding sites for biotinylated neoglycoproteins and heparin (endogenous lectins) during cerebellar ontogenesis in the rat.

    abstract：:Endogenous carbohydrate-binding sites were studied during rat cerebellar development on sections of fixed tissue using synthetic tools, biotinylated neoglycoproteins, in conjunction with subsequent avidinperoxidase staining. Neoglycoproteins were constructed by chemically coupling the histochemically pivotal carbohydr...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Kuchler S,Zanetta JP,Vincendon G,Gabius HJ

    更新日期：1990-06-01 00:00:00

  • Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules.

    abstract：:Monoclonal antibodies to the constitutive desmosomal glycoprotein desmoglein were characterized whose epitopes are located intracellularly, i.e., in the cytoplasmic portion of this molecule, and contribute to the structure of the desmosomal plaque. Using one of these antibodies (DG3.10), a peptide was isolated from a ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Koch PJ,Walsh MJ,Schmelz M,Goldschmidt MD,Zimbelmann R,Franke WW

    更新日期：1990-10-01 00:00:00

  • Ultrastructural detection of fucosyl residues at the surface of axenically grown maize roots/sequential use of UeA lectin and fucosyl ferritin as the specific glycosylated marker.

    abstract：:This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the slime droplet enshe...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Rougier M

    更新日期：1984-05-01 00:00:00

  • Molecular cloning of mouse connexins26 and -32: similar genomic organization but distinct promoter sequences of two gap junction genes.

    abstract：:Connexins26 and -32 are subunit proteins of gap junctions that are coexpressed in hepatocytes and several tissues but individually expressed in other cells. Molecular cloning of both corresponding mouse genes revealed similar genomic organization, i.e., each gene consists of two exons with the complete coding region l...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Hennemann H,Kozjek G,Dahl E,Nicholson B,Willecke K

    更新日期：1992-06-01 00:00:00

  • Suppression of transcription factor NF-kappaB activity by Bcl-2 protein in NIH3T3 cells: implication of a novel NF-kappaB p50-Bcl-2 complex for the anti-apoptotic function of Bcl-2.

    abstract：:Bcl-2 can suppress apoptosis by controlling genes that encode proteins required for programmed cell death and by interference with peroxidative damage. Overexpression of Bcl-2 in NIH3T3 cells can prevent GSNO-induced (S-nitrosoglutathione-induced) apoptosis. The experimental results indicated that activation of NF-kap...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/s0171-9335(04)70014-x

    authors： Hour TC,Chen L,Lin JK

    更新日期：2000-02-01 00:00:00

  • Gap junctions and rhombic particle arrays in the freeze-fractured mouse oocyte-follicle cell complex.

    abstract：:Intact follicles as well as defolliculated oocytes of the mouse were studied by freeze-fracture electron microscopy. In intact follicles the oocyte plasma membrane shows two prominent types of intra-membrane particle array:gap junctions and yet undescribed rhombic particle arrays. The gap junctions vary in size (from ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： van den Hoef MH,Bluemink JG,de Laat SW

    更新日期：1984-11-01 00:00:00

  • Cell motility during wound healing in giant algal cells: contraction in detergent-permeabilized cell models of Ernodesmis.

    abstract：:Triton-treated cells of the coenocytic green alga, Ernodesmis verticillata, manifest longitudinal contraction and centripetal closure of the cytoplasm in the presence of exogenous free calcium ions. Complete closure of the cytoplasm around the vacuolar remnants is obtained with 1 mM ATP present in the activation mediu...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： La Claire JW2nd

    更新日期：1984-03-01 00:00:00

  • Rapid reduction of MDCK cell cholesterol by methyl-beta-cyclodextrin alters steady state transepithelial electrical resistance.

    abstract：:The role of plasma membrane lipids in regulating the passage of ions and other solutes through the paracellular pathway remains controversial. In this study we explore the contribution of cholesterol (CH) in maintaining the barrier function of an epithelial cell line using the CH-solubilizing agent methyl beta-cyclode...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/s0171-9335(99)80074-0

    authors： Francis SA,Kelly JM,McCormack J,Rogers RA,Lai J,Schneeberger EE,Lynch RD

    更新日期：1999-07-01 00:00:00

  • Uptake and apparent digestion of cytoplasmic organelles by protein bodies (protein storage vacuoles) in mung bean cotyledons.

    abstract：:The large protein bodies of the storage parenchyma cells of mung bean (Vigna radiata) cotyledons contain vesicles measuring 0.2 to 2.0 mum in diameter. The vesicles contain ribosomes, ribosomes, membranous elements which may be derived from the endoplasmic reticulum and occasionally Golgi bodies and mitochondria. The ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Herman EM,Baumgartner B,Chrispeels MJ

    更新日期：1981-06-01 00:00:00

  • Periodic mechanical stress enhances rat chondrocyte area expansion and migration through Src-PLCgamma1-ERK1/2 signaling.

    abstract：:The signal transduction pathways of chondrocyte area expansion and migration under periodic mechanical stress remain a matter of debate. We explore this question by performing cell culture experiments in our self-developed periodic stress field and perfusion culture system. Under periodic mechanical stress, we find th...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2010.04.005

    authors： Nong L,Yin G,Ren K,Tang J,Fan W

    更新日期：2010-09-01 00:00:00

  • Absence of fodrin (spectrin-like protein) under the pseudopod membrane in stimulated thyroid cells.

    abstract：:A fodrin-like protein purified from porcine thyroid cells and characterized by its properties identical to those of pig brain spectrin (F. Regnouf et al., Eur. J. Biochem. 153, 313-319 (1985)) has been localized by immunofluorescence and electron immunocytochemistry in porcine and rat thyroid. Fodrin-like polypeptides...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Gabrion JB,Barrière H,Nguyen Than Dao B,Chambard M,Mauchamp J,Regnouf F,Pradel LA

    更新日期：1990-08-01 00:00:00

  • Colocalization of stomatin (band 7.2b) and actin microfilaments in UAC epithelial cells.

    abstract：:Cytolocalization of stomatin, an integral membrane protein also called erythrocyte band 7.2b, was investigated in a human epithelial cell line in which the expression of this protein is up-regulated after treatment with interleukin-6 and dexamethasone. A monoclonal antibody against stomatin was used to perform immunof...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Snyers L,Thinès-Sempoux D,Prohaska R

    更新日期：1997-07-01 00:00:00

  • Identification of tubulin-binding proteins of the chicken erythrocyte plasma membrane skeleton which colocalize with the microtubular marginal band.

    abstract：:The plasma membrane of nucleated erythrocytes contains a microtubular marginal band which appears to be associated with the plasma membrane skeleton. In this report, we identify two families of cytoskeletal proteins which may be involved in such an association. These proteins, of molecular mass 78 kDa and 48 kDa on SD...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Stetzkowski-Marden F,Deprette C,Cassoly R

    更新日期：1991-02-01 00:00:00

  • Estimation of Golgi membrane flow rates in ovary glands of aptenia cordifolia using cytochalasin B.

    abstract：:Ovary gland cells of Aptenia cordifolia were exposed to 100 micrograms/ml cytochalasin B (cyt B) for 30 or 60 min during the phase of granulocrine polysaccharide secretion. The drug caused a congestion of Golgi vesicles around the dictyosomes, probably resulting from an inhibition of the vesicle migration towards the ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Kristen U,Lockhausen J

    更新日期：1983-01-01 00:00:00

  • Thyroid cell proliferation in response to forced expression of gap junction proteins.

    abstract：:Gap junctions are known to play a role in the control of cell proliferation, and connexins (Cx) are considered to be tumor suppressors. However, the effects of Cx on cell proliferation are dependent on the Cx which is expressed and on the cell type under consideration. We previously found that restoration of cell-to-c...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00245

    authors： Flachon V,Tonoli H,Selmi-Ruby S,Durand C,Rabilloud R,Rousset B,Munari-Silem Y

    更新日期：2002-05-01 00:00:00

  • A heterogeneous tRNA granule structure exhibiting rapid, bi-directional neuritic transport.

    abstract：:mRNA translation is regulated by diverse mechanisms that converge at the initiation and elongation steps to determine the rate, profile, and localization of proteins synthesized. A consistently relevant feature of these mechanisms is the spatial re-distribution of translation machinery, a process of particular importa...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2018.02.002

    authors： Pilotte J,Chan SW,Farnum JB,Thomas WM,Smilansky Z,Vanderklish PW

    更新日期：2018-04-01 00:00:00

  • Expression of small heat shock proteins HspB2, HspB8, Hsp20 and cvHsp in different tissues of the perinatal developing pig.

    abstract：:Recently, we have described the developmental expression of the small heat shock proteins (sHsps) Hsp27/HspB1 and alphaB-crystallin/HspB5 in different tissues of pigs from almost full-term foetuses to three years old adults (P. Tallot, J. F. Grongnet, J. C. David, Biol. Neonate, 83, 281-288, 2003). The data described ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00337

    authors： Verschuure P,Tatard C,Boelens WC,Grongnet JF,David JC

    更新日期：2003-10-01 00:00:00

  • A formin-g role during development and disease.

    abstract：:Several different protein families were shown to be involved in the regulation of actin filament formation and have been studied extensively in processes such as cell migration. Among them are members of the formin family, which tend to promote the formation of linear actin filaments. Studies in recent years, often us...

    journal_title：European journal of cell biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ejcb.2013.11.004

    authors： Randall TS,Ehler E

    更新日期：2014-05-01 00:00:00

  • Regulation of protein secretion by crinophagy in perfused rat liver.

    abstract：:We examined the secretion of three serum proteins, albumin (RSA), alpha 2 mu-globulin (alpha 2 mu G), and transferrin (Trf), in the isolated perfused liver. Within 4 h of perfusion, only 20 to 35% of previously synthesized proteins were secreted by the liver into the recirculating medium. Low temperature inhibited the...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Lenk SE,Fisher DL,Dunn WA Jr

    更新日期：1991-12-01 00:00:00

  • Alterations of nucleolar ultrastructure and ribosome biogenesis by actinomycin D. Implications for U3 snRNP function.

    abstract：:We have studied, at the electron microscope level, the reorganizations of nucleolar ultrastructure induced by actinomycin D (AMD) in different conditions of drug treatment associated with an inhibition of rRNA synthesis. We have analyzed in parallel the localizations of ribosomal genes, of their transcripts, of variou...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Puvion-Dutilleul F,Mazan S,Nicoloso M,Pichard E,Bachellerie JP,Puvion E

    更新日期：1992-06-01 00:00:00

  • Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): implications for a specific role in mammalian myoblast differentiation.

    abstract：:NAC (nascent polypeptide associated complex) is a heterodimer consisting of an α and a β subunit. skNAC (skeletal and heart muscle-specific form of αNAC) is a variant of αNAC, which is induced in muscle differentiation and regeneration. We show here that skNAC expression is regulated by p38 MAPK, which plays a crucial...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2011.10.004

    authors： Berger F,Berkholz J,Breustedt T,Ploen D,Munz B

    更新日期：2012-02-01 00:00:00

  • Plakophilins--hard work in the desmosome, recreation in the nucleus?

    abstract：:The linkage of the different types of cytoskeletal proteins to cell adhesion structures at the cytoplasmic membrane and the connection of these contact sites to corresponding sites of adjacent cells is a prerequisite for integrity and stability of cells and tissues. The structurally most prominent types of such cell-c...

    journal_title：European journal of cell biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ejcb.2004.12.020

    authors： Schmidt A,Jäger S

    更新日期：2005-03-01 00:00:00

  • A simplified protocol for the isolation and culture of cardiomyocytes and progenitor cells from neonatal mouse ventricles.

    abstract：:The neonatal heart is a very useful tool for the study of biochemical pathways and properties of cardiomyocytes and as it has the potential to regenerate for a brief period of time from birth; it is also useful to study cardiac regeneration. However, as the heart matures, this proficiency for regeneration is reduced. ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2015.06.009

    authors： Vidyasekar P,Shyamsunder P,Santhakumar R,Arun R,Verma RS

    更新日期：2015-10-01 00:00:00

  • Calmodulin colocalization with cold-stable and nocodazole-stable microtubules in living PtK1 cells.

    abstract：:To investigate the association of calmodulin (CaM) with microtubules (MTs) in the mitotic apparatus (MA), the distributions of both CaM and tubulin were examined in mitotic PtK1 cells in which MT subclasses had been selectively removed or altered by treatment with cold or with the MT inhibitor, nocodazole. A fluoresce...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Sweet SC,Welsh MJ

    更新日期：1988-10-01 00:00:00

  • Cysteine proteinases in GH4C1 cells, a rat pituitary tumor cell line, are secreted by the constitutive and regulated secretory pathways.

    abstract：:Secretory granules of GH4C1 cells, a rat pituitary tumor cell line, are known to be induced by the treatment of estradiol (E2), insulin, and epidermal growth factor (EGF). We examined changes in the localization of cathepsins B, H, and L, lysosomal cysteine proteinases, in GH4C1 cells before and after hormonal treatme...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Waguri S,Sato N,Watanabe T,Ishidoh K,Kominami E,Sato K,Uchiyama Y

    更新日期：1995-08-01 00:00:00

  • Cell cycle-dependent assembly and disassembly of cytoplasmic microtubules in the plasmodium of the myxomycete Physarum polycephalum.

    abstract：:The giant syncytium of Physarum plasmodia possesses a complex cytoplasmic microtubule network except during the occurrence of the intranuclear mitosis. In early prophase stages, intranuclear spindles assemble concomitantly as the cytoplasmic microtubule network disassembles. No cytoplasmic microtubules are present in ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Salles-Passador I,Moisand A,Wright M

    更新日期：1992-06-01 00:00:00