Abstract:
:Endogenous carbohydrate-binding sites were studied during rat cerebellar development on sections of fixed tissue using synthetic tools, biotinylated neoglycoproteins, in conjunction with subsequent avidinperoxidase staining. Neoglycoproteins were constructed by chemically coupling the histochemically pivotal carbohydrate moieties to an inert carrier protein. The sugar part of the neoglycoproteins included common constituents of the carbohydrate part of cellular glycoconjugates, namely mannose, galactose, fucose, N-acetyl-glucosamine, N-acetylgalactosamine and N-acetyl-neuraminic acid to probe for the presence of respective endogenous receptors. Heparin was biotinylated after mild cyanogen bromide activation and aminoalkylation. Specific positive reactions were obtained for all neoglycoproteins and heparin. The staining pattern with the individual probes disclosed variable developmental regulation. Consequently, these results suggest that recognition processes during cerebellar development may include several types of carbohydrate determinants. In two instances, the binding of neoglycoproteins could be compared to endogenous lectin-specific antibodies. Despite a significant extent of accordance the comparison revealed notable differences. These differences were attributed primarily to fixation and the presence of physiological ligands that can mask the active endogenous carbohydrate-binding proteins. In any case, histochemical application of labeled neoglycoproteins is valuable to discern the presence, localization and developmental pattern of binding sites for the carbohydrate part of glycoconjugates, on which further biochemical and cell biological studies can consequently be based.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Kuchler S,Zanetta JP,Vincendon G,Gabius HJsubject
Has Abstractpub_date
1990-06-01 00:00:00pages
87-97issue
1eissn
0171-9335issn
1618-1298journal_volume
52pub_type
杂志文章abstract::Using an exogenous kinase, nuclear ribonucleoprotein complexes with sedimentation coefficients greater than 100S were phosphorylated in vitro before and after treatment with increasing concentrations of NaC1. The phosphorylation pattern of the proteins before raising the NaC1 concentration shows a major group of label...
journal_title:European journal of cell biology
pub_type: 杂志文章
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abstract::The presence of glutamylated tubulin, a widespread posttranslational modification of alpha- and beta-tubulin, has been investigated in Drosophila melanogaster using the specific monoclonal antibody GT335. We show here that this modification is strongly detected in brain and testis whereas other tissues analyzed did no...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1995-05-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1994-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1993-10-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1994-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1979-06-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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