Abstract:
:Triton-treated cells of the coenocytic green alga, Ernodesmis verticillata, manifest longitudinal contraction and centripetal closure of the cytoplasm in the presence of exogenous free calcium ions. Complete closure of the cytoplasm around the vacuolar remnants is obtained with 1 mM ATP present in the activation medium. The cytoplasmic contractions in these cell models closely resemble normal wound healing motility phenomena previously reported for this organism, thereby providing a system for studying the contractions associated with cellular wound healing. Dye-exclusion experiments with eosin B reveal that the tritonized models are freely permeable, whereas untreated cells do not take up the stain. Ultrastructurally, activated (contracting) models possess a network of microfilaments beneath the plasma membrane remnants in regions of active contraction. Microtubules are associated with the microfilaments, but they also occur throughout the periphery of the entire cells, even in unactivated models. This suggests that microfilaments may be involved in localized contractions leading to wound healing, whereas microtubules are probably providing a cytoskeletal framework upon which the microfilaments act. Colchicine (5 mM) has no effect on contraction in the models; extensive experiments with cytochalasins B and D (up to 100 micrograms/ml) have failed to show regular, reproducible inhibition of contraction also. Trifluoperazine (10 microM) or La3+ (1 mM) decreases the rate and magnitude of contraction to some extent, perhaps by preventing Ca2+ interaction with the contractile apparatus. The threshold concentration of free Ca2+ necessary to activate contraction in the models appears to be 10(-6) M, an order of magnitude higher than the preliminary value reported for non-permeabilized, wounded cells. Free Mg2+ also must be present for activation of models, with a minimum concentration of ca. 5 mM. The requirement of ATP for contraction can be demonstrated by the fact that either excessive washing of cell models prior to activation or inclusion of uncoupling agents in the solutions largely prevents activation of the models unless ATP is added to the Ca2+ -containing activation medium. These results provide further evidence that cellular wound healing in Ernodesmis is similar to the majority of other non-muscle cell motility phenomena in requiring ATP and micromolar amounts of free Ca2+ and that microfilaments are implicated in the contractile process. Moreover, the Ernodesmis cell models should be excellent systems for the further probing of cytoplasmic contractions associated with wound healing at the cellular level of organization.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
La Claire JW2ndsubject
Has Abstractpub_date
1984-03-01 00:00:00pages
180-9issue
2eissn
0171-9335issn
1618-1298journal_volume
33pub_type
杂志文章abstract::Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1985-05-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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abstract::During myocardial infarction, it is well known that the cytoplasm of cardiac myocytes becomes more acidic due to lactate accumulation. The resulting necrosis is believed to result, at least in part, from the leakage of lysosomal enzymes into the cytoplasm. In addition, it has previously been shown that cytoplasm acidi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1982-10-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1993-04-01 00:00:00
abstract::The release of newly synthesized neuropeptides was studied in an in vitro system using the adipokinetic hormone (AKH)-producing cells of an insect (Locusta migratoria) as a model system. Tritiated phenylalanine incorporated into three hormonal neuropeptides, AKH I, II and III, was used to distinguish newly synthesized...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1997-01-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70033-3
更新日期:2000-05-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Mouse epidermal melanoblasts preferentially proliferated from disaggregated epidermal cell suspensions derived from newborn mouse skin in serum-free medium (MPM) supplemented with dibutyryl adenosine 3', 5' cyclic monophosphate (DBcAMP) and basic fibroblast growth factor (bFGF). After 12 days, almost all keratinocytes...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:European journal of cell biology
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doi:
更新日期:1995-01-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1993-06-01 00:00:00