Abstract:
:The transition in mouse teratocarcinomas of pluripotential stem cells to histogenetically determined ones of the neurogenic cell lineage was analysed at the total cellular protein level by two-dimensional gel electrophoresis. The change in morphology and function was found to be reflected by extensive shifts in the protein synthesis patterns. From a total of about 1000 resolved polypeptides that are synthesized in embryoid bodies of a multidifferentiating teratocarcinoma, about 12% (117 proteins) are not found or only present at a very much reduced level in two teratocarcinoma-derived neuroblastomas. On the other hand, the change in phenotype is accompanied by the de novo or greatly enhanced synthesis of another 69 proteins (about 7%) in the neurogenic tumors. In a screening for differentiation-specific proteins this set was compared with the protein synthesized in neural tissue of maturing brain and muscle tissue of developing limbs at three different postimplantation stages covering the onset of organogenesis. This comparison disclosed that a great portion of the differentiation-related proteins (26 proteins) are synthesized in both brain and muscle and are probably required by a differentiated cell in general like cytostructural proteins. Seventeen polypeptides, however, are synthesized in a cell type specific manner. In particular, 4 proteins that are synthesized in both neurogenic tumors and in brain but not in muscle tissue were tentatively called nerve-specific proteins (NSP) and are the most promising in further analyses of differentiation-specific proteins.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Blüthmann Hsubject
Has Abstractpub_date
1985-09-01 00:00:00pages
254-62issue
2eissn
0171-9335issn
1618-1298journal_volume
38pub_type
杂志文章abstract::The plasma membrane of nucleated erythrocytes contains a microtubular marginal band which appears to be associated with the plasma membrane skeleton. In this report, we identify two families of cytoskeletal proteins which may be involved in such an association. These proteins, of molecular mass 78 kDa and 48 kDa on SD...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::This electron microscopic study demonstrates that formation of a functional eukaryotic 40S initiation complex is accompanied by conformational changes which obscure the characteristic structural features of the 40S ribosomal subunits and of the initiation factor eIF-3, the only macromolecular components of the complex...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::The aim of this study was to evaluate the influence of peroxisome proliferator-activated receptor γ (PPARγ) on self-renewal of mouse embryonic stem cells (mESCs) in the presence and absence of leukemia inhibitory factor (LIF). We demonstrated that in the presence of LIF, the activation of PPARγ by Rosiglitazone led to...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2013.03.003
更新日期:2013-04-01 00:00:00
abstract::Studies on Ehrlich ascites tumor cells enriched in different cell cycle compartments by centrifugal elutriation have shown that after exclusion of oxygen, G1- and early S-phase cells are restricted from further cell cycle progression. On addition of a balanced mixture of deoxypyrimidine and deoxypurine nucleosides to ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::The inner core of lipopolysaccharide (LPS) structures in Gram-negative bacteria is considered a highly conserved region. The sugar connecting the membrane-associated lipid A moiety with the hydrophilic saccharide moiety, 3-deoxy-alpha-d-manno-oct-2-ulosonic acid (Kdo) is present in every LPS molecule investigated but ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2009.10.002
更新日期:2010-01-01 00:00:00
abstract::p120 catenin (p120ctn) is required for the stability of classic cadherins at the cell surface and is thought to play a central role in modulating cell-cell adhesion. Cytoplasmic p120ctn promotes cell motility, and probably other activities, by modulating the activities of RhoA, Rac and Cdc42. E-cadherin is expressed i...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2016.10.003
更新日期:2016-12-01 00:00:00
abstract::Mouse pituitary neurointermediate lobes were pulse-incubated in [3H] arginine or [3H] lysine for 10 min and then chase-incubated for periods 0 to 4h. The labeled peptides from the lobes were analysed by immunoprecipitation with specific antisera, and thereafter, by acid-urea polyacrylamide gel electrophoresis. Using t...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::Appreciable amounts of glycosaminoglycans have been found by immunocytochemistry within mature elastin fibers of human dermis. On thin sections, elastin fibers showed antigenic sites for monoclonal antibodies recognizing the unsaturated units remaining after digestion of hyaluronic acid with Streptomyces hyaluronidase...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract::Adoptive T cell therapy is the transfer of T cells to a patient in order to combat disease. This procedure is mainly being used but not limited to the treatment of viral infections and malignancies including virus-associated tumors. Depending on the clinical context, the T cell donor may be the same patient or another...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2011.04.001
更新日期:2012-01-01 00:00:00
abstract::Specific antibodies to a protein designated P1 (Mr approximately equal to 63,000), which is specifically altered in mutants resistant to the microtubule inhibitor podophyllotoxin, bind to mitochondria in cells of various vertebrate and invertebrate species (Eur. J. Cell Biol. 44, 278-285 (1987); Can. J. Biochem. Cell ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::Previous studies have shown that caffeine treatment at 20 degrees C causes the intermediate compartment protein p58 to redistribute from the Golgi region without affecting the localization of the Golgi stack protein mannosidase II (J. Jäntti, E. Kuismanen, J. Cell Biol. 120, 1321-1335 (1993). Here we have dissected fu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Macrocyst formation in the cellular slime moulds is a sexual process induced under dark and humid conditions. Normal development life cycle in these organisms involves proliferation by cell division and, upon starvation, formation of multicellular aggregates and fruiting bodies, consisting of spores and stalk cells. M...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2006.05.012
更新日期:2006-09-01 00:00:00
abstract::This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80041-1
更新日期:1998-01-01 00:00:00
abstract::The fine structure of a joint receptor (R10) in a spider leg (Zygiella x-notata) was examined with light and electron microscopy. The R10 receptor consists of a compact ganglion which is situated near the dorsal joint membrane of the femur/patella joint. Each of the ten sensory cells comprising the ganglion sends one ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::The nuclear lamins are karyophilic proteins located at the nucleoplasmic surface of the inner nuclear membrane. We have constructed mutants immediately N-terminal to the nuclear localization signal of human lamin A to identify sites regulating the nuclear transport of the protein. Using an in vitro transport assay, we...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::The linkage of the different types of cytoskeletal proteins to cell adhesion structures at the cytoplasmic membrane and the connection of these contact sites to corresponding sites of adjacent cells is a prerequisite for integrity and stability of cells and tissues. The structurally most prominent types of such cell-c...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2004.12.020
更新日期:2005-03-01 00:00:00
abstract::Filensin is a lens-specific intermediate filament protein, expressed in the lens fiber cells but not the lens epithelium. Using antibodies to filensin and the other lens intermediate filament proteins, vimentin and CP49, the codistribution of filensin with CP49 and independence of this network from the vimentin networ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequen...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::Fucoidan is a sulfated polysaccharide extracted from brown seaweeds. It has anticoagulant and antithrombotic properties and inhibits, as well as heparin, vascular smooth muscle cell growth. In this study, we investigated, in the presence of serum and human recombinant growth factors, the effects of fucoidan and hepari...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80094-0
更新日期:1998-12-01 00:00:00
abstract::Mouse plasma cells have been exposed to a wide range of soluble and adsorptive macromolecular tracers for 10 min to 4 h to explore the possibility of membrane recycling related to the high secretory rate of these nonregulated secretory cells. Electron microscopic examination showed in all cases labeling of multivesicu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::Endogenous carbohydrate-binding sites were studied during rat cerebellar development on sections of fixed tissue using synthetic tools, biotinylated neoglycoproteins, in conjunction with subsequent avidinperoxidase staining. Neoglycoproteins were constructed by chemically coupling the histochemically pivotal carbohydr...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::Perfused Chara cells capable of resuming ATP-dependent cytoplasmic streaming in low free Ca++ solutions have been examined by electron microscopy for myosin-like filaments. Filaments 44 nm in diameter and up to 3 micron in length have been found associated with the endoplasmic reticulum that along with mitochondria, m...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract::The tumor-promoting drug 4 beta-phorbol 12-myristate 13-acetate (TPA) causes the loss of myofibrils in primary cultures of chick embryonic myotubes [9]. When myofibrils in chick myotubes are dispersed by TPA treatment (5 X 10(-8) M), there remains a class of non-myofibrillar actin filaments that are sensitive to subse...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::Initial integrin-mediated cell-matrix adhesions (focal complexes) appear underneath the lamellipodia, in the regions of the "fast" centripetal flow driven by actin polymerization. Once formed, these adhesions convert the flow behind them into a "slow", myosin II-driven mode. Some focal complexes then turn into elongat...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2005.11.001
更新日期:2006-04-01 00:00:00
abstract::The microtubule-associated protein 2 (MAP2) and its juvenile splicing variant MAP2c contain a phosphorylation site at Ser136 which is part of a Ser-Pro motif. This site lies within the N-terminal region common to MAP2b and MAP2c. It has been mapped by site-directed mutagenesis of recombinant MAP2c and by a monoclonal ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::We have studied, at the electron microscope level, the reorganizations of nucleolar ultrastructure induced by actinomycin D (AMD) in different conditions of drug treatment associated with an inhibition of rRNA synthesis. We have analyzed in parallel the localizations of ribosomal genes, of their transcripts, of variou...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Centromeres support the assembly of the kinetochore on every chromosome and are therefore essential for the proper segregation of sister chromatids during cell division. Centromere identity is regulated epigenetically through the presence of the histone H3 variant CENP-A. CENP-A regulation and incorporation specifical...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2011.04.016
更新日期:2011-10-01 00:00:00
abstract::LIM kinase (LIMK) plays a critical role in stimulus-induced remodeling of the actin cytoskeleton by linking signals from the Rho family GTPases to changes in cofilin activity. Recent studies have shown an important role for LIMK1 signaling in tumor cell invasion through regulating actin dynamics. In this study, we inv...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00382
更新日期:2004-08-01 00:00:00
abstract::In solid body tissues, environmental biomechanics is indispensable for tissue homeostasis. While characteristics of homeostasis include morphogenesis, proliferation and differentiation, the influences through biomechanics in corneal keratinocytes are poorly understood. Here we show for the first time that corneal kera...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2011.08.005
更新日期:2011-12-01 00:00:00
abstract::Exocytosis of synaptic vesicles (SV) results in the surface exposure of lumenal epitopes of SV proteins. We have recently described the use of antibodies directed against the lumenal N-terminus of synaptotagmin I (Sytlum-Abs) to morphologically monitor exo-endocytic recycling of SVs. We report here that a radioimmunoa...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00