Abstract：
:Seminal roots are initiated at the scutellar node during maize (Zea mays L.) embryo development. The maize mutant rtcs (rootless concerning crown and seminal roots) does not initiate seminal roots while its wild-type siblings form on average 2.9 seminal roots per seedling. In this study, proteome profiles of 25-day-old immature maize embryos were compared between wild-type and rtcs plants via two-dimensional electrophoresis (2-DE). Electrospray ionization tandem mass spectrometry (ESI-MS/MS) identified 23 proteins encoded by 21 different genes that were differentially accumulated between wild-type and rtcs embryos (Fc> or =2; FDR<10%). Among the differentially accumulated proteins, two isoforms of a phosphoglycerate kinase and a malate dehydrogenase were preferentially accumulated in wild-type embryos. Both enzymes are related to the generation of energy-rich ATP or NADPH molecules and are crucial checkpoints of cellular energetics in plants. Comparison of embryonic proteins differentially accumulated between wild-type and rtcs embryos revealed little overlap with proteins differentially accumulated between wild-type and rum1 embryos which also do not initiate seminal roots. This might be due to distinct influences of RTCS and RUM1 on the composition of the embryo proteome, but could also be explained by different stages of embryo development that were analyzed in these studies.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Muthreich N,Schützenmeister A,Schütz W,Madlung J,Krug K,Nordheim A,Piepho HP,Hochholdinger Fdoi
10.1016/j.ejcb.2009.11.014subject
Has Abstractpub_date
2010-02-01 00:00:00pages
242-9issue
2-3eissn
0171-9335issn
1618-1298pii
S0171-9335(09)00343-4journal_volume
89pub_type
杂志文章abstract：:Changes in the surface morphology of regenerating rabbit, rat and frog corneal endothelial cells in vivo have been investigated by scanning electron microscopy. In adult tissue these cells do not normally divide unless given a stimulus, such as injury. Surfaces of quiescent rabbit and rat cells are devoid of microvill...
journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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更新日期：1979-10-01 00:00:00
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更新日期：1982-08-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1986-10-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1996-10-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1990-12-01 00:00:00
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更新日期：1993-06-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1995-09-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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doi：
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journal_title：European journal of cell biology
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doi：
更新日期：1994-06-01 00:00:00
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