Abstract:
:Epidermal Langerhans cells (LC) play a key role in initiation and regulation of immune responses. Whereas LC migration out of the epidermis upon environmental assault is extensively studied, the mechanisms involved in the (re)population of the epidermis with LC are poorly understood. Here, we investigated the immigration of LC derived from the human MUTZ-3 cell line (MUTZ-LC) into the epidermis of a full thickness skin equivalent, comprising a fully differentiated epidermis on a fibroblast-populated dermis. MUTZ-LC were used to determine which epidermis-derived chemokines play a role in mediating LC trans-dermal migration into the epidermis. We found evidence for a role of keratinocyte-derived CCL5 and CCL20 in the chemo-attraction of MUTZ-LC. Neutralizing antibodies against CCL5 and CCL20 blocked LC migration towards keratinocytes. Secretion of these two chemokines was associated with incorporation of MUTZ-LC into the epidermis of full thickness skin equivalents. In conclusion, our findings suggest that epidermis derived CCL5 and CCL20 are pivotal mediators in recruitment of LC into the epidermis.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Ouwehand K,Spiekstra SW,Waaijman T,Breetveld M,Scheper RJ,de Gruijl TD,Gibbs Sdoi
10.1016/j.ejcb.2012.06.004subject
Has Abstractpub_date
2012-10-01 00:00:00pages
765-73issue
10eissn
0171-9335issn
1618-1298pii
S0171-9335(12)00115-Xjournal_volume
91pub_type
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