p21(Cip1) regulates cell-substrate adhesion and interphase microtubule dynamics in untransformed human mammary epithelial cells.

Abstract:

:Despite its frequent inactivation in human breast cancers, the role of p21(Cip1) (p21) in morphological plasticity of normal mammary epithelial cells is still poorly understood. To address this question, we have investigated the consequences of p21 silencing in two-dimensional (2D) morphogenesis of untransformed human mammary epithelial cells. Here we show that p21 inactivation causes a reduction of 2D cell spreading and suppresses focal adhesion. In order to investigate the cytoskeletal modifications associated with this altered morphology, we have analyzed the microtubule dynamics in interphase p21-depleted cells. Our results demonstrate that interphase microtubule dynamic instability is strongly increased by p21 silencing. This alteration correlates with severe microtubule hypoacetylation. Next, we show that these microtubule defects in p21-depleted cells can be reversed by the use of the small molecule tubacin, a specific inhibitor of the α-tubulin deacetylase HDAC6. Tubacin-induced microtubule dynamics decrease also correlates with a partial recovery of cell spreading and focal adhesion in those cells. Collectively, these data indicate that p21 regulates the morphological plasticity of normal mammary epithelial cells by modulating dynamics of key cytoskeletal components.

journal_name

Eur J Cell Biol

authors

Bouchet BP,Fauvet F,Grelier G,Galmarini CM,Puisieux A

doi

10.1016/j.ejcb.2011.03.002

subject

Has Abstract

pub_date

2011-08-01 00:00:00

pages

631-41

issue

8

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(11)00056-2

journal_volume

90

pub_type

杂志文章
  • Rab3 dissociation and clathrin-mediated endocytosis, two key steps in the exo-endocytotic pathway of large dense-cored vesicles in primary cultures of superior cervical ganglia.

    abstract::In this study we have used primary cultures of porcine superior cervical ganglia as a model system to study exo-endocytosis in sympathetic neurons. Pure neuronal cultures with a defined noradrenergic phenotype can be obtained when antimitotics are included in the culture medium, and the high yield from prenatal piglet...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Annaert WG,Partoens P,Slembrouck D,Bakker A,Jacob W,De Potter WP

    更新日期:1997-11-01 00:00:00

  • Fine structure of a spider joint receptor and associated synapses.

    abstract::The fine structure of a joint receptor (R10) in a spider leg (Zygiella x-notata) was examined with light and electron microscopy. The R10 receptor consists of a compact ganglion which is situated near the dorsal joint membrane of the femur/patella joint. Each of the ten sensory cells comprising the ganglion sends one ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Foelix RF,Choms A

    更新日期:1979-06-01 00:00:00

  • Effects of daunomycin on the microtubular network: a cytochemical study on a human melanoma cell line.

    abstract::The interaction of daunomycin (DAU), an anthracyclinic antibiotic employed as antitumoral agent, with microtubules, has been investigated by cytochemical and morphological methods on a human melanoma cell line (H14). Results obtained indicated that DAU was able to modulate the microtubule reassembly in cells treated w...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Molinari A,Calcabrini A,Crateri P,Arancia G

    更新日期:1991-04-01 00:00:00

  • Preferential release of newly synthesized, exportable neuropeptides by insect neuroendocrine cells and the effect of ageing of secretory granules.

    abstract::The release of newly synthesized neuropeptides was studied in an in vitro system using the adipokinetic hormone (AKH)-producing cells of an insect (Locusta migratoria) as a model system. Tritiated phenylalanine incorporated into three hormonal neuropeptides, AKH I, II and III, was used to distinguish newly synthesized...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Sharp-Baker HE,Oudejans RC,Kooiman FP,Diederen JH,Peute J,Van der Horst DJ

    更新日期:1996-09-01 00:00:00

  • New in situ approaches to study the induction of pollen embryogenesis in Capsicum annuum L.

    abstract::The induction of pollen embryogenesis in Capsicum annuum L. has been studied at the cellular level using various in situ approaches with several molecular probes for DNA, RNA and proteins. The late vacuolated microspore and the young bicellular pollen grain are stages of gametophytic development in which embryogenesis...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: González-Melendi P,Testillano PS,Ahmadian P,Fadón B,Risueño MC

    更新日期:1996-04-01 00:00:00

  • Protein segregation in peripheral 15 degrees C intermediates in response to caffeine treatment.

    abstract::Previous studies have shown that caffeine treatment at 20 degrees C causes the intermediate compartment protein p58 to redistribute from the Golgi region without affecting the localization of the Golgi stack protein mannosidase II (J. Jäntti, E. Kuismanen, J. Cell Biol. 120, 1321-1335 (1993). Here we have dissected fu...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Jäntti J,Saraste J,Kuismanen E

    更新日期:1997-10-01 00:00:00

  • Inhibition of RhoGAP activity is sufficient for the induction of Rho-mediated actin reorganization.

    abstract::It is generally believed that the induction of actin cytoskeleton rearrangements by extracellular stimuli results from the activation of guanine nucleotide exchange factors for the Rho GTPases. Here, we present evidence that the inactivation of RhoGAP (GTPase activating protein) activity is an equally effective means ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/S0171-9335(99)80019-3

    authors: Vincent S,Settleman J

    更新日期:1999-08-01 00:00:00

  • Induction of megamitochondria by cuprizone(biscyclohexanone oxaldihydrazone). Evidence for an inhibition of the mitochondrial division process.

    abstract::1. Feeding weanling mice a diet containing 0.5% (w/w) cuprizone (biscyclohexanone oxaldihydrazone) induced megamitochondria of a different type of that previously described (Suzuki, K., Science 163, 81-82 (1969). 2. As in the normal controls, a single population of mitochondria with a narrow size distribution, was det...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Flatmark T,Kryvi H,Tangerås A

    更新日期:1980-12-01 00:00:00

  • Multimeric complexes of differentiation-inducing protein bound to DNA.

    abstract::Myeloid hematopoietic precursor cells are induced to differentiate by the macrophage and granulocyte differentiation-inducing protein MGI-2 (DF). This differentiation-inducing protein bound to double-stranded but not to single-stranded mammalian DNA. The bound MGI-2 was not eluted by high salt, but was eluted by sodiu...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Weisinger G,Korn AP,Sachs L

    更新日期:1985-05-01 00:00:00

  • Phagosome maturation and fusion with lysosomes in relation to surface property and size of the phagocytic particle.

    abstract::Phagosomes with pathogenic mycobacteria, or with hydrophobic polystyrene beads of 1 micron in size, do not mature, but remain fusogenic towards early endosomes and do not fuse with lysosomes (de Chastellier et al. Eur. J. Cell Biol. 68, 167-182 (1995)). Both types of phagocytic particles display a close apposition to ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: de Chastellier C,Thilo L

    更新日期:1997-09-01 00:00:00

  • Phagosome fusion vesicles of paramecium. I. Thin-section morphology.

    abstract::Ultrastructural studies of the digestive system of Paramecium caudatum focusing on the first 5 min of digestive-vacuole age reveal a set of vesicles, named phagosome fusion vesicles (PFVs), which fuse with the digestive vacuole just after the vacuoles are released from the cytopharynx and concomitant with vacuole acid...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Allen RD,Fok AK

    更新日期:1983-01-01 00:00:00

  • [Biochemical demonstration of HCO3--und Cl--dependent ATPase activities in the rectum of larval dragonflies and inhibition of rectal chloride uptake by thiocyanate (author's transl)].

    abstract::Hydrogencarbonate and chloride activated, ouabain-insensitive ATPase activities are demonstrated in the salt-absorbing rectum of larval dragonflies. Maximal activation is achieved at approx. 30 mM HCO3- and 20 mM Cl-, respectively. The stimulation of each anion obeys Michaelis-Menten kinetics Km values are 4.65 mM for...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Komnick H,Schmitz M,Hinssen H

    更新日期:1980-02-01 00:00:00

  • An actin-modulating protein from Physarum polycephalum. I. Isolation and purification.

    abstract::High-speed centrifugation supernatants from slime mould plasmodia show considerable activities to inhibit the polymerization of actin as revealed by viscosity measurements. By following increasing inhibitory activities an actin modulating protein (AM-protein) has been isolated and purified which affects the polymer st...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Hinssen H

    更新日期:1981-02-01 00:00:00

  • Cellular graviperception in the basidiomycete Flammulina velutipes--can the nuclei serve as fungal statoliths?

    abstract::In search for the cellular mechanisms of graviperception in basidiomycete fruit bodies, nuclear and vacuole motility was investigated in the fungus, Flammulina velutipes. In this organism, hyphal nuclei are closely linked with the actin cytoskeleton, which is involved in gravity perception. Active motion of the nuclei...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Monzer J

    更新日期:1996-10-01 00:00:00

  • Endocytosis, intracellular transport, and turnover of anionic and cationic proteins in cultured mouse peritoneal macrophages.

    abstract::It was previously shown that cultured mouse peritoneal macrophages ingest anionic derivatives of horseradish peroxidase (HRP) and ferritin by fluid-phase endocytosis and accumulate them in lysosomes. Cationic derivatives were taken up by adsorptive endocytosis and transported to lysosomes but subsequently appeared als...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Stenseth K,Hedin U,Thyberg J

    更新日期:1983-07-01 00:00:00

  • Isolation and identification of actin from the phytopathogenic filamentous fungus uromyces appendiculatus.

    abstract::Crude protein extracts of Uromyces appendiculatus contain a polypeptide that resembles actin in several ways. This protein eluates from DEAE-cellulose with concentrations of KCl known to release actin of other species from the cation. The polypeptide is recognized by polyclonal antibodies directed to sodium dodecyl su...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Tiburzy R,Hoch HC,Staples RC

    更新日期:1990-12-01 00:00:00

  • Cytochemical localization of lipases during spore germination from Bryum capillare.

    abstract::In the oil containing spores of Bryum capillare, lipid breakdown occurs within three days after sowing. The periods of activity of acid, neutral and alkaline lipases are coincident. They start at the first day after sowing the spores. After 3 days of germination, when storage fats forming large globules are mobilised ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Carrapiço F,Salomé M,Pais S

    更新日期:1983-03-01 00:00:00

  • Immunofluorescence localization of the 90-kDa heat-shock protein to cytoskeleton.

    abstract::The 90-kDa heat-shock protein, hsp90, is an abundant and conserved protein located predominantly in the cytoplasm. Previous reports have localized a portion of the hsp90 to microtubules and to microfilaments. Here, we show that colocalization of hsp90 with microtubules is seen after long-term but not short-term fixati...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Czar MJ,Welsh MJ,Pratt WB

    更新日期:1996-08-01 00:00:00

  • Ectopic expression of prelamin A in early Xenopus embryos induces apoptosis.

    abstract::Lamin proteins are components of metazoan cell nuclei. During evolution, two classes of lamin proteins evolved, A- and B-type lamins. B-type lamins are expressed in nearly all cell types and in all developmental stages and are thought to be indispensable for cellular survival. In contrast, A-type lamins have a more re...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2008.06.001

    authors: Peter A,Stick R

    更新日期:2008-11-01 00:00:00

  • Association of the nuclear matrix component NuMA with the Cajal body and nuclear speckle compartments during transitions in transcriptional activity in lens cell differentiation.

    abstract::The transcriptional status of cells can be deduced from the staining pattern of various nuclear markers such as the Cajal body, nucleolus and nuclear speckles. In this study we have used these markers to correlate transcriptional status with cell differentiation in the lens. As a closed system with no cell loss and wi...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00275

    authors: Gribbon C,Dahm R,Prescott AR,Quinlan RA

    更新日期:2002-10-01 00:00:00

  • ATP requirement for induced tight junction formation in HT 29 adenocarcinoma cells.

    abstract::Tight junctions (TJ) of the fascia occludens type can be rapidly assembled in the human colon adenocarcinoma cell line HT 29 under the influence of trypsin or ammonium sulfate. We have studied the influence of the metabolic inhibitors, dinitrophenol (DNP) and deoxyglucose (DG), on the induced formation of TJ in this c...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Mückter H,Ben-Shaul Y,Bacher A

    更新日期:1987-10-01 00:00:00

  • Characterization of gap junction genes expressed in F9 embryonic carcinoma cells: molecular cloning of mouse connexin31 and -45 cDNAs.

    abstract::In an attempt to characterize connexin genes expressed early in mouse development we screened a cDNA library from mouse F9 embryonic carcinoma cells with mouse connexin37 cDNA and mouse connexin31.1 genomic DNA under low stringency of hybridization. We detected 5 different connexin cDNAs coding for mouse connexins31, ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Hennemann H,Schwarz HJ,Willecke K

    更新日期:1992-02-01 00:00:00

  • The apical localization of SGLT1 glucose transporter is determined by the short amino acid sequence in its N-terminal domain.

    abstract::SGLT1, an isoform of Na+-dependent glucose cotransporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney, where it plays a pivotal role in the absorption and reabsorption of sugars, respectively. To search the domain responsible for the apical localization of ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00204

    authors: Suzuki T,Fujikura K,Koyama H,Matsuzaki T,Takahashi Y,Takata K

    更新日期:2001-12-01 00:00:00

  • Surface movements during the spreading of blood platelets.

    abstract::When human blood platelets spread on a substratum they increase their surface area as much as 4-fold. We investigated the mechanism of spreading by light microscopy and by scanning and transmission electron microscopy. Contact of a platelet with a glass surface induces formation of thin extensions which spread out ove...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Behnke O,Bray D

    更新日期:1988-06-01 00:00:00

  • A nuclear lamin of the nematode Caenorhabditis elegans with unusual structural features; cDNA cloning and gene organization.

    abstract::This report describes the characterization of the nuclear lamin CeLam-1 of the nematode Caenorhabditis elegans by molecular analysis of the corresponding complete cDNA and gene sequences. The primary structure of CeLam-1, representing only the third non-vertebrate lamin sequence currently known, follows essentially th...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Riemer D,Dodemont H,Weber K

    更新日期:1993-12-01 00:00:00

  • A caudal-type homeobox gene activity associated with the development and regeneration of the liver.

    abstract::Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Doll U,Niessing J

    更新日期:1996-07-01 00:00:00

  • AP-1/c-Jun transcription factors: regulation and function in malignant melanoma.

    abstract::Malignant melanoma is an aggressive form of skin cancer with an increasing incidence worldwide. One way to address the pathology of the disease is through molecular research. In addition to the analysis of melanoma-relevant signaling pathways, the investigation of important transcription factors is a fundamental objec...

    journal_title:European journal of cell biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ejcb.2013.10.003

    authors: Kappelmann M,Bosserhoff A,Kuphal S

    更新日期:2014-01-01 00:00:00

  • Plasma cell endocytosis: is it related to immunoglobulin secretion?

    abstract::Mouse plasma cells have been exposed to a wide range of soluble and adsorptive macromolecular tracers for 10 min to 4 h to explore the possibility of membrane recycling related to the high secretory rate of these nonregulated secretory cells. Electron microscopic examination showed in all cases labeling of multivesicu...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Tartakoff A,Vassalli P,Montesano R

    更新日期:1981-12-01 00:00:00

  • The fate of multilamellar bodies produced and secreted by Dictyostelium discoideum amoebae.

    abstract::The amoeba Dictyostelium discoideum produces and secretes multilamellar bodies (MLBs) mainly composed of amoebal membranes upon digestion of bacteria. After their secretion, the fate of these MLBs remains unknown. The aim of this study was to determine if protozoa can internalize and digest secreted D. discoideum MLBs...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2017.10.002

    authors: Denoncourt AM,Durocher AF,Paquet VE,Charette SJ

    更新日期:2017-12-01 00:00:00

  • Heterogeneity of the zymogen granule membranes in rat pancreas.

    abstract::Zymogen granules (ZG) of rat pancreas have been isolated by the procedure of Pâquet et al. The granules lysed when exposed to alkaline pH (pH 8.2), and their membranes could be subfractionated by centrifugation on a sucrose gradient. Four discrete types of membranes corresponding to densities of 1.105, 1.085, 1.075, a...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Beaudoin AR,Gilbert L,St-Jean P,Grondin G,Cabana C

    更新日期:1988-12-01 00:00:00