Abstract:
:The tumor-promoting drug 4 beta-phorbol 12-myristate 13-acetate (TPA) causes the loss of myofibrils in primary cultures of chick embryonic myotubes [9]. When myofibrils in chick myotubes are dispersed by TPA treatment (5 X 10(-8) M), there remains a class of non-myofibrillar actin filaments that are sensitive to subsequent breakdown by cytochalasin D. Microfilament bundles in fibroblasts in the same cultures seem unaffected by this TPA treatment, but are also broken down by cytochalasin D (0.2 micrograms/ml); this dose has little effect on myofibrils. We have previously shown that treatment of chick myotubes with cytochalasins would destabilize clusters of acetylcholine receptors (AChRs) [6]. In order to further examine the relationship between actin filaments and cell surface AChRs, we have used the receptor-specific ligand alpha bungarotoxin (a-BGT) to study the fate of AChR clusters in drug-treated and control myotubes. Cells treated with TPA showed no loss in the number of receptor clusters present on their surface. However, if these cells were also treated with cytochalasin D, cluster number was reduced to approximately the same value as seen for cytochalasin treatment alone (50% of the control value). These results suggest that the cytoskeletal link to these cell surface receptors is not mediated by attachment to the alpha actin-containing myofibrils, but rather clustered AChRs are stabilized by a class of non-myofibrillar actin filaments.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Connolly JA,Graham AJsubject
Has Abstractpub_date
1985-05-01 00:00:00pages
191-5eissn
0171-9335issn
1618-1298journal_volume
37pub_type
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::Cultured fetal rat hepatocytes derived from 12, 15 and 19-day gestation rats are capable of secreting transferrin. When dexamethasone is added to the medium an increased secretion rate is observed. The changes in secretion rates in control as well as dexamethasone-treated cells during culture have been shown to correl...
journal_title:European journal of cell biology
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doi:
更新日期:1986-10-01 00:00:00
abstract::Colorectal cancer is a major health problem worldwide with urgent need for new and effective anti-cancer approaches that allow treating, increasing survival and improving life quality of patients. At pharmacological concentrations, ascorbic acid (AA) exerts a selective cytotoxic effect, whose mechanism of cytotoxicity...
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
abstract::A remarkable amount, of water is transported in the gastrointestinal (GI) organs to fulfil the secretory and absorptive functions of the GI tract. However, the molecular basis of water movement in the GI epithelial barriers is still poorly known. Important clues about the mechanisms by which water is transported in th...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:2010-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2010.04.007
更新日期:2011-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80112-X
更新日期:1998-11-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::Lysosomal Ca2+ release channel TRPML1 has been suggested to regulate lysosome size by activating calmodulin (CaM). To further understand how TRPML1 and CaM regulate lysosome size, in this study, we report that inhibiting mTORC1 causes enlarged lysosomes, and the recovery of enlarged lysosomes is suppressed by inhibiti...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2019.05.001
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/s0171-9335(99)80034-x
更新日期:1999-11-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(99)80099-5
更新日期:1999-03-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80014-9
更新日期:1998-05-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(99)80055-7
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00