Abstract：
:The uptake mechanism of homologous IgG and immune complex, and the participation of coated vesicles in this process were studied in rat peritoneal macrophages. Peroxidase-antiperoxidase (PAP) immune complex produced in rat, and purified rat IgG adsorbed to gold particles (IgG-Au) were used as ligands. Freshly collected peritoneal macrophages were preincubated with the ligands at 4 degrees C, washed, warmed up to 37 degrees C, maintained in a serum-free culture medium for 5 sec to 30 min and subsequently fixed for electron microscopy. In the IgG-Au experiments, acid phosphatase reaction was also applied to identify lysosomes, and ruthenium red to trace membranes exposed to the extracellular space. At the end of the preincubation period PAP and IgG were found randomly distributed on the external surface of the plasma membrane. After warming up the cells to 37 degrees C, the ligands bound to the plasma membrane showed a tendency to move towards deep labyrinthic invaginations of the cell surface from where they were internalized via coated pits and coated vesicles. In the initial period, these structures seemed to be the primary carriers of the ligands. In the period between 5 and 10 min, ligands were concentrated in vacuoles (endosomes) located in the deeper cytoplasm, while after 30 min, they were present in large lysosome-like or multivesicular bodies, which were found to be acid phosphatase positive.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Kiss AL,Röhlich Psubject
Has Abstractpub_date
1984-05-01 00:00:00pages
88-95issue
1eissn
0171-9335issn
1618-1298journal_volume
34pub_type
杂志文章abstract：:The microtubule-associated protein 2 (MAP2) and its juvenile splicing variant MAP2c contain a phosphorylation site at Ser136 which is part of a Ser-Pro motif. This site lies within the N-terminal region common to MAP2b and MAP2c. It has been mapped by site-directed mutagenesis of recombinant MAP2c and by a monoclonal ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Lysosomal Ca2+ release channel TRPML1 has been suggested to regulate lysosome size by activating calmodulin (CaM). To further understand how TRPML1 and CaM regulate lysosome size, in this study, we report that inhibiting mTORC1 causes enlarged lysosomes, and the recovery of enlarged lysosomes is suppressed by inhibiti...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2019.05.001
更新日期：2019-06-01 00:00:00
abstract：:The major histocompatibility complex (MHC) class II-associated invariant chain (Ii) contains signals for transport to endocytic compartments where the class II molecules bind antigenic peptides for presentation to CD4+ T cells. Two leucine-based signals in the Ii cytoplasmic tail can be independently recognized for en...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80014-9
更新日期：1998-05-01 00:00:00
abstract：:We examined the secretion of three serum proteins, albumin (RSA), alpha 2 mu-globulin (alpha 2 mu G), and transferrin (Trf), in the isolated perfused liver. Within 4 h of perfusion, only 20 to 35% of previously synthesized proteins were secreted by the liver into the recirculating medium. Low temperature inhibited the...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:The mammalian skin has a photosensitive system comprised by several opsins, including rhodopsin (OPN2) and melanopsin (OPN4). Recently, our group showed that UVA (4.4 kJ/m2) leads to immediate pigment darkening (IPD) in murine normal and malignant melanocytes. We show the role of OPN2 and OPN4 as UVA sensors: UVA-indu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2018.01.004
更新日期：2018-04-01 00:00:00
abstract：:In search for the cellular mechanisms of graviperception in basidiomycete fruit bodies, nuclear and vacuole motility was investigated in the fungus, Flammulina velutipes. In this organism, hyphal nuclei are closely linked with the actin cytoskeleton, which is involved in gravity perception. Active motion of the nuclei...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:Human enteric α-defensins (HD5 and HD6), major antimicrobial peptides produced by Paneth cells in the intestine, play important roles in intestinal innate immunity. Since their expression is decreased in Crohn's disease (CD), with decreased expression being more pronounced in the presence of NOD2 mutations, it would b...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2014.10.007
更新日期：2015-01-01 00:00:00
abstract：:The differences in the content of trace elements (Fe, Cu, Zn, Br, Rb and Sr) in phasic and tonic scallop adductor muscles have been revealed by using X-ray fluorescence analysis. The phasic (yellow) muscles have been found to be richer in Fe and Cu while more deficient in Zn and Br as compared to the tonic (white) mus...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:The exhaustion of metabolically poisoned muscle fibres is accompanied by a one hundred-fold increase in potassium conductance. Under these conditions a normal membrane capacity was measured. Extracellular markers (lanthanum, ferritin) were traced in the transverse tubular system but not in the myoplasm or the sarcopla...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:The signal transduction pathways of chondrocyte area expansion and migration under periodic mechanical stress remain a matter of debate. We explore this question by performing cell culture experiments in our self-developed periodic stress field and perfusion culture system. Under periodic mechanical stress, we find th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.04.005
更新日期：2010-09-01 00:00:00
abstract：:The high-affinity receptor (Fc gamma RI) for the constant (Fc) portion of immunoglobulin G (IgG) is one of three Fc IgG receptor classes (Fc gamma Rs) found on mononuclear phagocytes. The functional specialization of each of the Fc gamma R classes is not well understood. Previous studies utilizing anti-Fc gamma R mono...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:By double indirect immunofluorescence and interference electron microscopy, we have observed the effect of microtubule disruption by antimitotic drugs and coldness treatment on the distribution of adhesion sites and of the three cytoskeletal proteins, vinculin, alpha-actinin, and actin in normal rat cells and in rat c...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:Previously we reported the novel observation that astrocytes ensheath the persistent hyaloid artery, both in the Nuc1 spontaneous mutant rat, and in human PFV (persistent fetal vasculature) disease (Developmental Dynamics 234:36-47, 2005). We now show that astrocytes isolated from both the optic nerve and retina of Nu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.01.003
更新日期：2011-05-01 00:00:00
abstract：:A new, two step method is described for the isolation of megakaryocytes from bone marrow. By means of centrifugation in a PercollR density gradient megakaryocytes can be enriched by factor 68 or 24 starting from rat or guinea pig bone marrow, respectively. The purity of the megakaryocytes can be improved ca. 4 fold if...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-06-01 00:00:00
abstract：:Pretreatment of Acanthamoeba castellanii (Neff strain) with sublytic concentrations of surface-active alkyltrimethylammonium salts (C12, C14, C16) enhanced ConA-mediated agglutination of the amoebae. Treatment with the surfactants alone did not affect the "spontaneous" agglutination of the amoebae. Electron microscopi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:The rabies virus (RV) phosphoprotein P is a multifunctional protein involved in viral RNA synthesis and in counteracting host innate immune responses. We have previously shown that RV P gene expression levels can be regulated by using picornavirus internal ribosome entry site (IRES) elements. Here we exploited a parti...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.01.009
更新日期：2012-01-01 00:00:00
abstract：:The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80009-0
更新日期：1999-02-01 00:00:00
abstract：:Translin is an evolutionarily conserved approximately 27-kDa protein that binds to specific DNA and RNA sequences and has diverse cellular functions. Here, we report the cloning and characterization of the translin orthologue from the fruit fly Drosophila melanogaster. Under protein-denaturing conditions, purified Dro...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2006.11.005
更新日期：2007-03-01 00:00:00
abstract：:Chondrocyte terminal differentiation is associated with cellular hypertrophy increased activity of plasma membrane alkaline phosphatase and the synthesis of collagen type X. The hypertrophic phenotype of cultured chondrocytes can be stimulated by ascorbic acid but the underlying mechanisms for this phenotypic change a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/s0171-9335(98)80023-x
更新日期：1998-06-01 00:00:00
abstract：:Proteins that are concentrated in specific compartments of the endomembrane system in order to exert their organelle-specific function must possess specific localization signals that prevent their transport to distal regions of the exocytic pathway. Some resident proteins of the endoplasmic reticulum (ER) that are kno...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/S0171-9335(04)70025-4
更新日期：2000-04-01 00:00:00
abstract：:This electron microscopic study demonstrates that formation of a functional eukaryotic 40S initiation complex is accompanied by conformational changes which obscure the characteristic structural features of the 40S ribosomal subunits and of the initiation factor eIF-3, the only macromolecular components of the complex...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:The murine recessive yellow (Mc1r(e)) is a loss-of-function mutation in the receptor for alpha-melanocyte-stimulating hormone, melanocortin receptor 1 (Mc1r) and produces yellow coats by inducing pheomelanin synthesis in hair follicular melanocytes. However, it is not known whether the Mc1r(e) mutation affects the pro...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2007.03.007
更新日期：2007-06-01 00:00:00
abstract：:Cytochrome b5 has been visualized by an immunoferritin labeling in a Golgi preparation. When the Golgi apparatus was first disrupted before labeling, 60% of the profiles were clearly labeled with ferritin. This proportion of labeled vesicles was too high to be explained by contamination from other membranes. When the ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-11-01 00:00:00
abstract：:Rabbit antisera against homogeneous rat liver thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) were prepared and used for immunohistochemical analysis in adult rats. Immunoreactive thioredoxin and thioredoxin reductase were widely distributed in tissues and organs, but va...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:The mechanisms by which adenosine regulates the inflammatory reaction are poorly characterized. In this study, we investigated the effects of adenosine on neutrophil actin polymerization elicited by the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) or IgG-opsonized yeast particles. We used bodipy-...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80055-1
更新日期：1998-02-01 00:00:00
abstract：:Various drugs added to the culture medium of rabbit mammary gland were assayed for their capacity to affect the lactogenic and the mammogenic activities of prolactin. Three lysosomotropic agents NH4Cl, chloroquine and methylamine which were previously demonstrated to inhibit the degradation of the hormone-receptor com...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:Despite its frequent inactivation in human breast cancers, the role of p21(Cip1) (p21) in morphological plasticity of normal mammary epithelial cells is still poorly understood. To address this question, we have investigated the consequences of p21 silencing in two-dimensional (2D) morphogenesis of untransformed human...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.03.002
更新日期：2011-08-01 00:00:00
abstract：:Studies on Ehrlich ascites tumor cells enriched in different cell cycle compartments by centrifugal elutriation have shown that after exclusion of oxygen, G1- and early S-phase cells are restricted from further cell cycle progression. On addition of a balanced mixture of deoxypyrimidine and deoxypurine nucleosides to ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:In the mouse genome, 20 connexin genes have been detected that code for proteins of high sequence identity in the two extracellular loops, especially six conserved cysteine residues. The mouse connexin23 (Cx23) gene (Gje1) differs from all other connexin genes in vertebrates, since it codes for a protein that contains...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2008.08.004
更新日期：2009-02-01 00:00:00
abstract：:Human cytomegalovirus (HCMV) infection causes a rapid, progressive disruption of the host cell cytoskeleton that correlates with actin depolymerization. Whole-mount (3D) electron microscopy was used to analyze the cytoskeleton of uninfected and HCMV-infected human lung fibroblast cells. Within 2 min of HCMV infection,...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
