Abstract：
AIMS:Hypoxia makes cells death but it is not always true. Short term anoxia can boost remaining cells activities and promote tissue repair. This study is to explore the effects of hypoxic preconditioning (HP) on osteoblasts and analyze the involved lncRNA and mRNA. MAIN METHODS:Human osteoblast was used. Normoxic control group (N) were cultured in normoxic culture medium (L-DMEM, 10% FBS and 1% penicillin-streptomycin) 8 h. Long-term hypoxic group (H) were cultured in 4 h hypoxic culture medium (200 μmol/L CoCl2 and L-DMEM)/4 h normoxic culture medium. Hypoxic preconditioning group (HP) were received 3 times HP (cultured in 10 min hypoxic culture medium/10 min normoxic culture medium, which was HP 1times) and then cultured in 4 h hypoxic culture medium/4 h normoxic culture medium. Cell proliferation ability, osteonectin, transforming growth factor-β1, insulin like growth factors-1 and osteopontin were test. The lncRNA and mRNA was detected by high-throughput sequencing. p-Value < 0.05 was accepted as statistically significant. KEY FINDINGS:Cell proliferation activity: N > HP > P. Corrected osteonectin, TGF-β1, IGF-1 and osteopontin: HP > P > N. High-throughput sequencing of gene shown the expression of 4630 RNAs. Further, we identified that HP group made 239 and 160 target genes up-regulated and down-regulated respectively compared with H group (p < 0.05). GO, KEGG and PPI analysis indicated relevant biological processes. SIGNIFICANCE:Hypoxic preconditioning alleviated cells anoxia tolerance and promoted cells to synthesis many cytokines. The protection mechanism involved many lncRNAs and mRNAs.
journal_name
Life Scijournal_title
Life sciencesauthors
Wang D,Liu Y,Yang X,Zhou Jdoi
10.1016/j.lfs.2018.07.014subject
Has Abstractpub_date
2018-09-01 00:00:00pages
46-54eissn
0024-3205issn
1879-0631pii
S0024-3205(18)30391-6journal_volume
208pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:The present study was designed to investigate the effect of partial (two-thirds) hepatectomy (PH) on hepatic and intestinal glutathione S-transferases (GSTs) activities. A significant decrease of cytosolic hepatic GSTs activity was observed after the PH. The lowest value of hepatic GSTs was obtained 48 h after the sur...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02024-d
更新日期：1995-01-01 00:00:00
abstract：:The aims of this study were to determine whether endothelin-1 (ET-1), a positive inotropic agent, altered the production of cyclic AMP (cAMP) in adult feline cardiac myocytes and to characterize the effect with respect to G-protein-coupling and calcium regulation of adenylyl cyclase. ET-1 inhibited basal and/or stimul...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02264-3
更新日期：1996-01-01 00:00:00
abstract：:Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma x glioma hybrid. Only...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01732-0
更新日期：2002-07-05 00:00:00
abstract：:Sodium-dependent transport of D-glucose has been reported only in epithelial cells of small intestine and kidney, and well-differentiated tumors thereof. We observed a two-fold decrease (p < 0.05) in the intracellular distribution volume (Vic, defined as steady-state intracellular uptake divided by extracellular conce...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00114-2
更新日期：1996-01-01 00:00:00
abstract：:Green tea polyphenols like epigallocatechin gallate (EGCG) have been proposed as a cancer chemopreventative. Several studies have shown that EGCG can act as an antioxidant by trapping proxyl radicals and inhibiting lipid peroxidation. The main propose of this study is to investigate the antioxidant capacity of EGCG us...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.08.019
更新日期：2004-02-06 00:00:00
abstract：:Inhaled antimuscarinics, often called anticholinergics in clinical medicine, are established as first line bronchodilators in COPD. Tiotropium has been developed as a new generation antimuscarinic following ipratropium. Tiotropium is a specific, highly potent antimuscarinic, demonstrating very slow dissociation from m...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(98)00588-8
更新日期：1999-01-01 00:00:00
abstract：:Anandamide (arachidonylethanolamide; AEA) is an endogenous ligand for the cannabinoid receptor and its pharmacological effects are under intensive study. However, AEA has a low aqueous solubility and stability which may restrict its use and may eventually endanger the reliability of the obtained results. In the presen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00024-0
更新日期：1996-01-01 00:00:00
abstract：:The outbreak of COVID-19 in December 2019, has become an urgent and serious public health emergency. At present, there is no effective treatment or vaccine for COVID-19. Therefore, there is a crucial unmet need to develop a safe and effective treatment for COVID-19 patients. Mesenchymal stem cells (MSCs) are widely us...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118493
更新日期：2020-12-01 00:00:00
abstract：:In the CNS, dopamine (DA) is a recognized neurotransmitter as well as a precursor for norepinephrine (NE) and epinephrine (EPI). In contrast to the CNS, DA has been assumed to be only a precursor in peripheral tissues. There is now, however, considerable evidence to support the hypothesis that it may function as a neu...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(83)90827-5
更新日期：1983-04-11 00:00:00
abstract：AIMS:Several off-label studies have shown that acamprosate can provide some clinical benefits in youth with Fragile X Syndrome (FXS), an autism spectrum disorder caused by loss of function of the highly conserved FMR1 gene. This study investigated the ability of acamprosate to rescue cellular, molecular and behavioral ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.01.007
更新日期：2018-02-15 00:00:00
abstract：:S-adenosylmethionine and adenosine levels in the rat pineal gland were determined by high-performance liquid chromatography after fractionation of the pineal extracts. The concentration of S-adenosylmethionine follows a circadian rhythm and is about three times higher during the day (2.5 nmol/gland) than the night (1....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90253-4
更新日期：1984-08-06 00:00:00
abstract：AIMS:Oxidative stress and inflammatory response are major factors causing several tissue injuries in intestinal ischemia and reperfusion (I/R). Agmatine has been reported to attenuate I/R injury of various organs. The present study aims to analyze the possible protective effects of agmatine on intestinal I/R injury in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.08.032
更新日期：2017-11-15 00:00:00
abstract：:In adult male Wistar rats submitted to a standardized noise stress, intravenous TRH induced a prolactin (PRL) secretory response. Prior IV naloxone administration not only lowered plasma PRL levels in those stressed rats but abolished also the stimulatory action of TRH. This effect was further studied by superfusion e...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90756-9
更新日期：1987-06-22 00:00:00
abstract：:The effect of the Type I benzodiazepine (BDZ) receptor agonist, CL 218,872, on convulsions generated by low doses of methyl beta-carboline-3-carboxylic acid ( MBCC ), bicuculline, picrotoxin and pentylenetetrazole (PTZ) in mice was examined. Low doses of CL 218,872 enhanced the convulsions produced by all agents excep...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90321-7
更新日期：1984-05-28 00:00:00
abstract：:The accumulation of lysophosphoglycerides has been implicated as an important biochemical factor for cardiac arrhythmias. Recently, we demonstrated that lysophosphatidylcholine caused cardiac arrhythmias in the isolated hamster heart. In this study, the arrhythmogenic nature of various lysophosphoglycerides with respe...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90806-8
更新日期：1983-03-21 00:00:00
abstract：AIM:Muscle atrophy is a common symptom after nerve denervation. Myostatin propeptide, a precursor of myostatin, has been documented to improve muscle growth. However, the mechanism underlying the muscle atrophy attenuation effects of myostatin propeptide in muscles and the changes in gene expression are not well establ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.12.056
更新日期：2016-02-01 00:00:00
abstract：:Interleukin-1 (IL-1) is a monokine which has been demonstrated to produce a variety of seemingly diverse metabolic events including fever, neutrophilia, anorexia, altered mineral metabolism, muscle catabolism, and fibroblast proliferation. Because many of the clinical features of alcoholic hepatitis are metabolic abno...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90554-0
更新日期：1986-10-20 00:00:00
abstract：:The hypoglycemic and hypolipidemic effects of Lycium barbarum fruit water decoction, crude polysaccharide extracts (crude LBP), and purified polysaccharide fractions (LBP-X) in alloxan-induced diabetic or hyperlipidemic rabbits were investigated through designed sequential trials and by measuring blood glucose and ser...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.04.056
更新日期：2004-11-26 00:00:00
abstract：:Endomorphin 1 (EM1) and endomorphin 2 (EM2) are highly potent and selective mu-opioid receptor agonists and have significant antinociceptive action. In the mu-selective pocket of endomorphins (EMs), Pro2 residue is a spacer and directs the Tyr1 and Trp3/Phe3 side chains into the required orientation. The present work ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.03.005
更新日期：2005-07-22 00:00:00
abstract：:Rhesus monkeys were trained to discriminate injections of saline from those of beta-carboline-3-carboxylic acid ethyl ester (beta-CCE), a compound that binds to the benzodiazepine receptor, but often has actions opposite to those of the benzodiazepines. A benzodiazepine agonist midazolam and low doses of a specific be...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90240-7
更新日期：1986-03-17 00:00:00
abstract：:The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]cis methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90602-6
更新日期：1982-03-08 00:00:00
abstract：AIMS:Currently, chronic gastritis is a high incidence of digestive diseases, along with loss of appetite, abdominal pain and diarrhea. Baicalin belongs to the major bioactive flavonoids compounds from Scutellariae Radix, it exhibited anti-inflammatory and anti-bacteria activities. Nonetheless, the protective effects of...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117064
更新日期：2019-12-15 00:00:00
abstract：:The interaction of cell surface hormone receptors with heterotrimeric G proteins is crucial for hormonal actions. The domains of the receptor, which interact with and activate G protein, have been extensively studied. However, precise molecular mechanisms underlying regulation of the receptor-induced G protein activat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.09.002
更新日期：2003-12-05 00:00:00
abstract：:alpha-Difluoromethylornithine (DFMO) directly infused into a brain-lateral ventricle (12.5, 25 and 50 micrograms/rat) dose- and time-dependently inhibited brain ODC activity. While having no influence per se on pain threshold, DFMO significantly inhibited the analgesic activity of morphine (15 mg/kg i.p.), this effect...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90614-0
更新日期：1989-01-01 00:00:00
abstract：:Intestinal mucosa, bone marrow hematopoietic cells and brain have limited capacity for the de novo synthesis of nucleosides (NSs) and nucleotides (NTs). Whereas the role of dietary NS and NT in the former two tissues is known, it is not known in the brain. Therefore we studied the effect of dietary NS and NT mixture o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00526-7
更新日期：1996-01-01 00:00:00
abstract：:A laboratory mouse can be partially restrained by placing it inside a cage with its tail protruding through a hole in one wall and taped to a surface outside of the cage. The mouse maintains enough mobility to perform an operant response yet its movements are limited to the extent that electrical or chemical stimulati...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90164-3
更新日期：1982-11-15 00:00:00
abstract：:Lipid peroxidation products were measured in the plasma of 24 kidney transplant patients and 12 healthy volunteers (controls) by: (1) 2-thiobarbituric acid assay and (2) the intensity of fluorescence products of malonaldehyde cross-linked proteins. Plasma levels of creatinine, ceruloplasmin, transferrin, prealbumin, a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90059-z
更新日期：1990-01-01 00:00:00
abstract：:We previously showed that N1-phenyl-3,5-dinitro-N4,N4-di-n-butylsulfanilamide (denoted GB-II-150) possesses selective antimicrotubule activity against Leishmania donovani and Trypanosoma brucei in vitro [Bhattacharya, G., Herman, J., Delfin, D., Salem, M.M., Barszcz, T., Mollet, M., Riccio, G., Brun, R., Werbovetz, K....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.03.028
更新日期：2006-08-08 00:00:00
abstract：:Rats implanted subcutaneously with an empty osmotic pump connected by a polyethylene catheter to a jugular vein for 5 to 10 days evinced a decreased splenocyte responsiveness to blastogenic stimulation in vitro to bacterial lipopolysaccharide, a known B cell stimulator, as well as to the plant mitogens pokeweed mitoge...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90291-v
更新日期：1992-01-01 00:00:00
abstract：:Gastric ulceration associated with the use of NSAIDs is most frequently observed in elderly women, the same sector of society most likely to be receiving therapy for osteoporosis. As some anti-osteoporosis medications have been suggested to irritate the upper gastrointestinal mucosa, we evaluated the ability of one su...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)01040-0
更新日期：1998-01-01 00:00:00
