Is intestinal cytosolic glutathione S-transferase an alternative detoxification pathway in two-thirds hepatectomized rats?


:The present study was designed to investigate the effect of partial (two-thirds) hepatectomy (PH) on hepatic and intestinal glutathione S-transferases (GSTs) activities. A significant decrease of cytosolic hepatic GSTs activity was observed after the PH. The lowest value of hepatic GSTs was obtained 48 h after the surgery. On the other hand, intestinal GSTs activities increased after PH, reaching the highest values 48 h after the hepatic lobes resection. The hepatic GSTs activities diminution was attributed, in part, to the high accumulation of bile acids in the liver tissue of hepatectomized rats, also demonstrated by a higher retention of [14C] taurocholate. The kinetic analysis performed with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate showed two sets of parameters, indicating the presence of isozymes of high and low affinities. Vmax1 and Vmax2 were lower in PH rats suggesting a non competitive inhibition mechanism. The inhibitory effect of bile acids decreased during liver regeneration process of hepatectomized rats disappearing at 7 days after PH. Conversely, in non regenerating rats (GABA treated) the inhibitory mechanism was still observed at 7 days after the surgery. The increase of intestinal GSTs activities (isozymes of high and low affinities) was attributed to the presence of polyamines, mainly putrescine, produced during the hepatic regeneration process. In this regard, it was showed that GABA treatment, which inhibits polyamine synthesis, completely abolished the increase on intestinal GSTs activities. Finally, the treatment with exogenous putrescine showed that in hepatectomized and sham-operated rats, the polyamine induced GSTs activities in both tissues. In PH rats, the putrescine dependent increase of hepatic GSTs was masked by the inhibitory effect of bile acids. In addition, a summation effect of endogenous and exogenous putrescine was probably the reason of the induction of intestinal GSTs after PH. The GSH/GSSG ratio did not change during the treatments, as well as the microsomal GST activity of both tissues. The work points out the hypothetical detoxification power of the intestine during the hepatocellular insufficiency which follows a two-thirds hepatectomy.


Life Sci


Life sciences


Carnovale CE,Monti JA,Favre C,Scapini C,Carrillo MC




Has Abstract


1995-01-01 00:00:00














  • Proinflammatory cytokine IFN-γ, lncRNA BANCR and the occurrence of coronary artery disease.

    abstract:AIMS:Coronary artery disease (CAD) ranks the leading cause of death globally. Interferon-γ (IFN-γ) gene, along with long noncoding RNA (lncRNA) BRAF-activated noncoding RNA (BANCR), could coordinately function in the occurrence of CAD. We hypothesized that level of IFN-γ, genetic variants of IFN-γ and BANCR gene should...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wang H,Zhang N,Li G,Xu B

    更新日期:2019-08-15 00:00:00

  • The protective effects of prolactin on brain injury.

    abstract:AIMS:Brain injuries based on their causes are divided into two categories, TBI and NTBI. TBI is caused by damages such as head injury, but non-physical injury causes NTBI. Prolactin is one of the blood factors that increase during brain injury. It has been assumed to play a regenerative role in post-injury recovery. M...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Yousefvand S,Hadjzadeh MA,Vafaee F,Dolatshad H

    更新日期:2020-12-15 00:00:00

  • Phospholipid liposomes and prolactin secretion: effects on spontaneous and drug-induced hyperprolactinaemia.

    abstract::Bovine brain phospholipid liposomes (BC-PL) reduce plasma prolactin (PRL) levels in humans after acute administration and counteract the metoclopramide- and sulpiride-induced hyperprolactinaemia. However, BC-PL, like nomifensine, a dopamine (DA) reuptake inhibitor and therefore an indirect dopaminergic compound, does ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Falaschi P,Rocco A,D'Urso R,Jellamo R,Toffano I,Frajese G

    更新日期:1982-07-05 00:00:00

  • Gossypol ameliorates liver fibrosis in diabetic rats induced by high-fat diet and streptozocin.

    abstract::11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) inhibitors have been shown to treat type 2 diabetes (T2D). Since gossypol is an 11β-HSD1 inhibitor, the objective of the present study was to treat T2D and T2D-related liver fibrosis in rat model using low-dose gossypol. T2D was induced by feeding with high fat diet plus i...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chen G,Wang R,Chen H,Wu L,Ge RS,Wang Y

    更新日期:2016-03-15 00:00:00

  • Gene expression of somatostatin receptor subtypes, SSTR1 and SSTR2, in human lung cancer cell lines.

    abstract::Somatostatin (SS) acts as a universal endocrine off-swich, and also inhibits the growth of neuroendocrine tumors through its specific receptors. Small cell lung cancer (SCLC) demonstrates some neuroendocrine characteristics and has been proposed as a candidate for treatment with SS and its analogues. In the present st...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Fujita T,Yamaji Y,Sato M,Murao K,Takahara J

    更新日期:1994-01-01 00:00:00

  • Differential effects of naloxone on basal and stress-induced release of ACTH and prolactin in the male rat.

    abstract::The effect of i.v. injection of various doses of naloxone (NAL) on plasma adrenocorticotropin (ACTH) and prolactin (Prl) in conscious animals bearing an indwelling intrajugular catheter was assessed. The effects were evaluated in animals which were left undisturbed and in others subjected to either restraint or ether ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Xu RK,McCann SM

    更新日期:1989-01-01 00:00:00

  • Inhibition of immune cell proliferation with haloperidol and relationship of tyrosine hydroxylase expression to immune cell growth.

    abstract::Previous studies indicated that exogenous dopamine and its agonists directly regulated mitogen-induced immune cell proliferation. In this study, we further investigated role of endogenous dopamine in immune cell growth. Haloperidol, a general antagonist for dopamine receptors, could reduce the cell growth rate of T ce...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Tsao CW,Lin YS,Cheng JT

    更新日期:1998-01-01 00:00:00

  • Effect of dietary nitric oxide on gastric mucosal mast cells in absence or presence of an experimental gastritis in rats.

    abstract::Synthetic nitric oxide donors are known to protect the gastric mucosa from damage and dietary nitrate is known to release NO in the stomach. Mast cells have been found to be involved in gastric mucosal damage in humans or in rodents, and recent studies have pointed out the possibility of nitric oxide from endogenous o...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Larauche M,Buéno L,Fioramonti J

    更新日期:2003-08-08 00:00:00

  • Tetrahydroaminoacridine affects the cholinergic function of blood-brain barrier.

    abstract::In rat brain microvessels, tetrahydroaminoacridine (THA) caused a significant inhibition of both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities, in a dose-dependent manner. THA resulted to be a more potent inhibitor of BuChE than AChE. Lineweaver-Burk plots showed that Km (app) and V were alt...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Catalán RE,Martínez AM,Aragonés MD,Miguel BG,Hernández F,Cruz E

    更新日期:1993-01-01 00:00:00

  • Evolution of the activity of UGT1A1 throughout the development and adult life in a rat.

    abstract::Biliary excretion is the main route of disposal of bilirubin and impaired excretion results in jaundice, a well recognisable symptom of liver disease. Conjugation of bilirubin in the liver is essential for its clearance. The glucuronidation of bilirubin is catalysed by the microsomal UDP-glucuronosyltransferase UGT1A1...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bustamante N,Cantarino MH,Arahuetes RM,Cubero FJ,Ortiz A

    更新日期:2006-03-06 00:00:00

  • Effects of some isoxazolpyrimidine derivatives on nitric oxide and eicosanoid biosynthesis.

    abstract::The inhibitory effect of some isoxazolpyrimidine derivatives on iNOS and COX-2 endotoxin induction in mouse peritoneal macrophages has been studied. Three of these compounds inhibited nitrite and PGE2 accumulation in a concentration dependent-manner at microM range. None of these active compounds affected iNOS, COX-2,...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Vidal A,Ferrándiz ML,Ubeda A,Guillén I,Riguera R,Quintela JM,Peinador C,Moreira MJ,Alcaraz MJ

    更新日期:2000-01-21 00:00:00

  • Subtypes of the muscarinic receptor in smooth muscle.

    abstract::Muscarinic receptors are expressed in smooth muscle throughout the body. In most instances, the muscarinic receptor population in smooth muscle is composed of mainly the M2 and M3 subtypes in an 80% to 20% mixture. The M3 subtype mediates phosphoinositide hydrolysis and calcium mobilization, whereas the M2 subtype med...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Ehlert FJ,Ostrom RS,Sawyer GW

    更新日期:1997-01-01 00:00:00

  • Allethrin interactions with the nicotinic acetylcholine receptor channel.

    abstract::Interactions of the synthetic pyrethroid allethrin with the nicotinic acetylcholine (ACh) receptor/channel were studied in membranes from Torpedo electric organ. Allethrin did not inhibit binding of [3H]ACh to the receptor sites, but inhibited noncompetitively binding of [3H]perhydrohistrionicotoxin ([3H]H12-HTX) to t...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Abbassy MA,Eldefrawi ME,Eldefrawi AT

    更新日期:1982-10-11 00:00:00

  • Electrophysiological mechanisms for the antiarrhythmic activities of naloxone on cardiac tissues.

    abstract::It has been reported that naloxone, an opioid antagonist, has antiarrhythmic activity in vivo. In Langendorff perfused rat hearts, we found that ischemia-reperfusion-induced ventricular tachyarrhythmia reverted to normal sinus rhythm after the treatment with naloxone (3 approximately 10 microM). The method of voltage ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Hung CF,Wu MH,Tsai CH,Chu SH,Chi JF,Su MJ

    更新日期:1998-01-01 00:00:00

  • The influence of NO-containing ruthenium complexes on mouse hippocampal evoked potentials in vitro.

    abstract::The influence of different, nitric oxide-containing ruthenium complexes on the evoked potentials recorded from the CA1 region of the mouse hippocampus in vitro has been investigated. Of the compounds tested, only trans-[(NO)(P(OEt)3)(NH3)4Ru](PF6)3 (1-2.5 mM) exerted a strong facilitatory action on the population spik...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wieraszko A,Clarke MJ,Lang DR,Lopes LG,Franco DW

    更新日期:2001-02-16 00:00:00

  • Is highly expressed ACE 2 in pregnant women "a curse" in times of COVID-19 pandemic?

    abstract::Angiotensin-converting enzyme 2 (ACE 2) is a membrane-bound enzyme that cleaves angiotensin II (Ang II) into angiotensin (1-7). It also serves as an important binding site for SARS-CoV-2, thereby, facilitating viral entry into target host cells. ACE 2 is abundantly present in the intestine, kidney, heart, lungs, and f...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Dhaundiyal A,Kumari P,Jawalekar SS,Chauhan G,Kalra S,Navik U

    更新日期:2021-01-01 00:00:00

  • Central depletion of dopamine in rats by 1-methyl-4-phenylpyridine.

    abstract::Effects of 1-methyl-4-phenylpyridine(MPP+), a putative neurotoxic metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP), on the contents of dopamine were examined in the various regions of the rat brain. Under anesthesia with pentobarbital sodium and flunitrazepam, MPP+ 150 micrograms/rat was intracerebrove...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Okada M,Yokotani K,Yamashita M,Ikeda H,Osumi Y

    更新日期:1989-01-01 00:00:00

  • Specific induction of a 72-kDa heat shock protein protects esophageal mucosa from reflux esophagitis.

    abstract:AIMS:The aim of this study is to investigate the expression and cytoprotective function of a 72-kDa heat shock protein (HSP72) using a reflux esophagitis model in rats. MAIN METHODS:Expression of HSP60, HSP72, and HSP90 in rat esophageal mucosa was evaluated by Western blot analysis before and after hyperthermia (42.5...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Izumi Y,Otaka M,Takahashi T,Takada M,Shimada Y,Asaoka D,Nagahara A,Itoh H,Watanabe S

    更新日期:2009-04-10 00:00:00

  • A novel effect of polymorphonuclear leukocytes in the facilitation of angiogenesis.

    abstract::The purpose of this study was to examine whether the adhesion of polymorphonuclear leukocytes (PMNs) to endothelial cells and/or reactive oxygen species (ROS) released from PMNs are responsible for inducing angiogenesis. Angiogenesis was assessed by tube formation using endothelial cells obtained from bovine thoracic ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yasuda M,Shimizu S,Tokuyama S,Watanabe T,Kiuchi Y,Yamamoto T

    更新日期:2000-04-14 00:00:00

  • Endothelial and vascular smooth muscle cell dysfunction mediated by cyclophylin A and the atheroprotective effects of melatonin.

    abstract:AIMS:This study evaluated the role of cyclophilin A (CyPA) in early phase of atherosclerosis and also examined the atheroprotective effects of melatonin due to its antioxidant properties. MAIN METHODS:APOE null mice at 6 and 15weeks of age were treated with melatonin at a dose of 0.1mg/kg/day or 10mg/kg/day. We evalua...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Rezzani R,Favero G,Stacchiotti A,Rodella LF

    更新日期:2013-05-20 00:00:00

  • Tramadol induces antidepressant-type effects in mice.

    abstract::Tramadol is a clinically-effective, centrally-acting analgesic. This drug is a racemic mixture of two enantiomers, each one displaying different mechanisms: (+)tramadol displays opioid agonist properties and inhibits serotonin reuptake while (-)tramadol inhibit preferentially noradrenaline reuptake. The action of tram...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Rojas-Corrales MO,Gibert-Rahola J,Micó JA

    更新日期:1998-01-01 00:00:00

  • Inflammatory cardiac fibroblast phenotype underlies chronic alcohol-induced cardiac atrophy and dysfunction.

    abstract:AIMS:The purpose of this study was to investigate mechanisms of chronic alcohol-induced cardiac remodeling and dysfunction. We also sought to determine the role of cardiac fibroblasts, which play a dynamic role in cardiac remodeling, in mediating these effects. MAIN METHODS:Adult male Wistar rats were exposed to ethan...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mouton AJ,El Hajj EC,Ninh VK,Siggins RW,Gardner JD

    更新日期:2020-03-15 00:00:00

  • An acute dose of nicotine does not inhibit MAO B in baboon brain in vivo.

    abstract::Tobacco smoke exposure has been shown to inhibit brain and platelet MAO B in animals and in humans. Though the mechanism(s) responsible for MAO B inhibition are not known, studies in rodents have shown that nicotine administration does not inhibit brain MAO B. In this study we investigated whether brain MAO B is also ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Fowler JS,Volkow ND,Logan J,Pappas N,King P,MacGregor R,Shea C,Garza V,Gatley SJ

    更新日期:1998-01-01 00:00:00

  • Impacts of icodextrin on integrin-mediated wound healing of peritoneal mesothelial cells.

    abstract:AIMS:Exposure to glucose and its metabolites in peritoneal dialysis fluid (PDF) results in structural alterations of the peritoneal membrane. Icodextrin-containing PDF eliminates glucose and reduces deterioration of peritoneal membrane function, but direct effects of icodextrin molecules on peritoneal mesothelial cells...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Matsumoto M,Tamura M,Miyamoto T,Furuno Y,Kabashima N,Serino R,Shibata T,Kanegae K,Takeuchi M,Abe H,Okazaki M,Otsuji Y

    更新日期:2012-06-14 00:00:00

  • Ethnic differences of coronary artery disease-associated SNPs in two Israeli healthy populations using MALDI-TOF mass spectrometry.

    abstract::Differences in prevalence and mortality from coronary artery disease (CAD) were observed among the different Israeli ethnic groups. The incidence of CAD in Israel is highest among Ashkenazi Jews and is much lower among Yemenite Jews. In this present study, we selected 15 single nucleotide polymorphisms (SNPs) from 14 ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Nakai K,Habano W,Nakai K,Fukushima N,Fujita T,Gurwitz D

    更新日期:2004-07-09 00:00:00

  • Hydrogen peroxide preconditioning protects PC12 cells against apoptosis induced by dopamine.

    abstract::Dopamine (DA), one of the major sources of reactive oxygen species (ROS), is implicated in neuronal death associated with Parkinson's disease (PD). Preconditioning with oxidative stress has been shown to provide cytoprotection similar to ischemic preconditioning (IPC), against cell apoptosis. In this study, using the ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Xiao-Qing T,Jun-Li Z,Yu C,Jian-Qiang F,Pei-Xi C

    更新日期:2005-11-19 00:00:00

  • Release of (14C)5-hydroxytryptamine from human platelets by red wine.

    abstract::Red wine, at a final dilution of 1/50, caused release of [14C]5-hydroxytryptamine (5-HT) from preloaded platelets, an effect which was not observed with any white wines or beers tested. Since 5-HT, is probably released from body stores during migraine attacks and red wine is known to provoke migraine episodes in susce...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Jarman J,Glover V,Sandler M

    更新日期:1991-01-01 00:00:00

  • Toxic effects of fluoride on organisms.

    abstract::Accumulation of excess fluoride in the environment poses serious health risks to plants, animals, and humans. This endangers human health, affects organism growth and development, and negatively impacts the food chain, thereby affecting ecological balance. In recent years, numerous studies focused on the molecular mec...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Zuo H,Chen L,Kong M,Qiu L,Lü P,Wu P,Yang Y,Chen K

    更新日期:2018-04-01 00:00:00

  • Vitamin E, membrane fluidity, and blood pressure in hypertensive and normotensive rats.

    abstract::Vitamin E treatment was found to lower blood pressure, and increase membrane fluidity in rats. The objectives of this study were to investigate the effects of the antioxidant, vitamin E, on the blood pressure and erythrocyte membrane fluidity in spontaneously hypertensive (SHR) and normotensive (WKY) rats. Membrane fl...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Pezeshk A,Derick Dalhouse A

    更新日期:2000-09-01 00:00:00

  • Pharmacological characterization of a vasopressin V1 receptor in the isolated human gastric artery.

    abstract::Species-related specific differences in the pharmacological profile of vasopressin V1a receptors have been reported. Thus, the aim of the present study was to identify a vascular preparation of human origin expressing V1a receptors. Vasopressin was found to contract human gastric artery strips without endothelium with...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Calò G,Rizzi A,Traina L,Regoli D

    更新日期:1997-01-01 00:00:00