当前位置： SCI文献检索 > Neural Regeneration Research期刊下所有文献 > The novel chalcone analog L2H17 protects retinal ganglion cells from oxidative stress-induced apoptosis.

The novel chalcone analog L2H17 protects retinal ganglion cells from oxidative stress-induced apoptosis.

Abstract：

:Chalcone is a plant metabolite widely found in fruits, vegetables, spices and tea, and has anti-tumor, anti-inflammation, immunomodulation, antibacterial and anti-oxidation activities, as well as many other pharmacological and biological effects. Our team has shown that its analogs have antioxidant activity, and oxidative stress is a pathological hallmark of retinal ischemia/reperfusion injury that can lead to retinal damage and visual loss. This investigation aims to identify a chalcone that protects retinal ganglion cells in vitro from the effects of oxidative stress and examine its mechanism. Rat retinal ganglion cell-5 cells were pretreated with chalcones and then exposed to tert-butyl hydroperoxide that causes oxidative damage. Controls received dimethyl sulfoxide only or tert-butyl hydroperoxide in dimethyl sulfoxide. Only (E)-3,4-dihydroxy-2'-methylether ketone (L2H17), of the five chalcone analogs, markedly increased the survival rate of oxidatively injured RGC-5 cells. Thus, subsequent experiments only analyzed the results of the L2H17 intervention. Cell viability and apoptosis were measured. Intracellular superoxide dismutase and reactive oxygen species levels were used to assess induced oxidative stress. The mechanism of action by L2H17 was explored by measuring the ER stress/UPR pathway and the expression and localization of Nrf2. All results demonstrated that L2H17 could reduce the apoptosis of oxidatively injured cells, inhibit caspase-3 activity, increase Bcl-2 expression, decrease Bad expression, increase the activity of superoxide dismutase, inhibit the production of reactive oxygen species, increase Nrf2 immunoreactivity, and reduce the activating transcription factor 4, phospho-eukaryotic initiation factor 2 and CHOP expression. L2H17 protects retinal ganglion cells induced by oxidative stress by regulating Nrf2, which indicates that it has the potential to become a drug for retinal ischemia/reperfusion.

journal_name

Neural Regen Res

journal_title

Neural regeneration research

authors

Wang L,Chen HC,Yang X,Tao JJ,Liang G,Wu JZ,Wu WC,Wang Y,Song ZM,Zhang X

doi

10.4103/1673-5374.237140

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

1665-1672

issue

eissn

1673-5374

issn

1876-7958

pii

NeuralRegenRes_2018_13_9_1665_237140

journal_volume

pub_type

杂志文章

在线工具

The novel chalcone analog L2H17 protects retinal ganglion cells from oxidative stress-induced apoptosis.