Abstract：
:Insulin-like growth factor 1 (IGF-1) is a potential nutrient for nerve repair. However, it is impractical as a therapy because of its limited half-life, rapid clearance, and limited target specificity. To achieve targeted and long-lasting treatment, we investigated the addition of a binding structure by fusing a collagen-binding domain to IGF-1. After confirming its affinity for collagen, the biological activity of this construct was examined by measuring cell proliferation after transfection into PC12 and Schwann cells using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Immunofluorescence staining was conducted to detect neurofilament and microtubule-associated protein 2 expression, while real time-polymerase chain reaction was utilized to determine IGF-1 receptor and nerve growth factor mRNA expression. Our results demonstrate a significant increase in collagen-binding activity of the recombinant protein compared with IGF-1. Moreover, the recombinant protein promoted proliferation of PC12 and Schwann cells, and increased the expression of neurofilament and microtubule-associated protein 2. Importantly, the recombinant protein also stimulated sustained expression of IGF-1 receptor and nerve growth factor mRNA for days. These results show that the recombinant protein achieved the goal of targeting and long-lasting treatment, and thus could become a clinically used factor for promoting nerve regeneration with a prolonged therapeutic effect.
journal_name
Neural Regen Resjournal_title
Neural regeneration researchauthors
Li JA,Zhao CF,Li SJ,Zhang J,Li ZH,Zhang Q,Yang XY,Zan CFdoi
10.4103/1673-5374.226400subject
Has Abstractpub_date
2018-02-01 00:00:00pages
298-303issue
2eissn
1673-5374issn
1876-7958pii
NeuralRegenRes_2018_13_2_298_226400journal_volume
13pub_type
杂志文章abstract：:This study aimed to summarize therapy experience of carotid endarterectomy, carotid endarterectomy combined with Fogarty catheter embolectomy, and hybrid surgery for the treatment of extracranial internal carotid artery occlusion. The study included 65 patients with extracranial internal carotid artery occlusion who u...
journal_title：Neural regeneration research
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abstract：:Severe edema in the endoneurium can occur after non-freezing cold injury to the peripheral nerve, which suggests damage to the blood-nerve barrier. To determine the effects of cold injury on the blood-nerve barrier, the sciatic nerve on one side of Wistar rats was treated with low temperatures (3-5°C) for 2 hours. The...
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pub_type： 杂志文章,评审
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更新日期：2014-06-01 00:00:00
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journal_title：Neural regeneration research
pub_type： 杂志文章,评审
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journal_title：Neural regeneration research
pub_type： 杂志文章
doi：10.3969/j.issn.1673-5374.2013.13.001
更新日期：2013-05-05 00:00:00
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journal_title：Neural regeneration research
pub_type： 杂志文章,评审
doi：10.4103/1673-5374.139454
更新日期：2014-08-01 00:00:00
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更新日期：2017-05-01 00:00:00
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更新日期：2014-11-01 00:00:00
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journal_title：Neural regeneration research
pub_type： 杂志文章
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更新日期：2013-05-25 00:00:00
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journal_title：Neural regeneration research
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pub_type： 杂志文章,评审
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