Synthetic, Chemically Defined Polymer-Coated Microcarriers for the Expansion of Human Mesenchymal Stem Cells.


:Mesenchymal stem cells (MSC), also called marrow stromal cells, are adult cells that have attracted interest for their potential uses in therapeutic applications. There is a pressing need for scalable culture systems due to the large number of cells needed for clinical treatments. Here, a tailorable thin polymer coating-poly(poly(ethylene glycol) methyl ether methacrylate-ran-vinyl dimethyl azlactone-ran-glycidyl methacrylate) [P(PEGMEMA-r-VDM-r-GMA); PVG]-to the surface of commercially available polystyrene and glass microcarriers to create chemically defined surfaces for large-scale cell expansion is applied. These chemically defined microcarriers create a reproducible surface that does not rely on the adsorption of xenogenic serum proteins to mediate cell adhesion. Specifically, this coating method anchors PVG copolymer through ring opening nucleophilic attack by amine residues on poly-l-lysine that is pre-adsorbed to the surface of microcarriers. Importantly, this anchoring reaction preserves the monomer VDM reactivity for subsequent functionalization with an integrin-specific Arg-Gly-Asp peptide to enable cell adhesion and expansion via a one-step reaction in aqueous media. MSCs cultured on PVG-coated microcarriers achieve sixfold expansion-similar to the expansion achieved on PS microcarriers-and retain their ability to differentiate after harvesting.


Macromol Biosci


Krutty JD,Dias AD,Yun J,Murphy WL,Gopalan P




Has Abstract


2019-02-01 00:00:00












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    abstract::5,6-Bis(benzylideneamino)-2-mercaptopyrimidin-4-ol (SCR7) is a new anti cancer molecule having capability to selectively inhibit non-homologous end joining (NHEJ), one of the DNA double strand break (DSB) repair pathways inside the cells. In spite of the promising potential as an anticancer agent, hydrophobicity of SC...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: John F,George J,Vartak SV,Srivastava M,Hassan PA,Aswal VK,Karki SS,Raghavan SC

    更新日期:2015-04-01 00:00:00

  • O-Mannosylation Affords a Glycopeptide Hydrogel with Inherent Antibacterial Activities against E. coli via Multivalent Interactions between Lectins and Supramolecular Assemblies.

    abstract::Multivalent carbohydrate-lectin interactions play a crucial role in bacterial infection. Biomimicry of multivalent glycosystems represents a major strategy in the repression of bacterial growth. In this study, a new kind of glycopeptide (Naphthyl-Phe-Phe-Ser-Tyr, NMY) scaffold with mannose modification is designed and...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Li J,Liang S,Yan Y,Tian X,Li X

    更新日期:2019-09-01 00:00:00

  • Transfection of mammalian cells using block copolypeptide vesicles.

    abstract::An arginine-leucine block copolypeptide (R60 L20 ) is synthesized, which is capable of forming vesicles with controllable sizes, able to transport hydrophilic cargo across the cell membrane, and exhibit relatively low cytotoxicity. The R60 L20 vesicles also possess the ability to deliver DNA into mammalian cells for t...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Sun VZ,Choe UJ,Rodriguez AR,Dai H,Deming TJ,Kamei DT

    更新日期:2013-05-01 00:00:00

  • Hybrid multicomponent hydrogels for tissue engineering.

    abstract::Artificial ECMs that not only closely mimic the hybrid nature of the natural ECM but also provide tunable material properties and enhanced biological functions are attractive candidates for tissue engineering applications. This review summarizes recent advances in developing multicomponent hybrid hydrogels by integrat...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章,评审


    authors: Jia X,Kiick KL

    更新日期:2009-02-11 00:00:00

  • Crosslinking of trimethylene carbonate and D, L-lactide (co-) polymers by gamma irradiation in the presence of pentaerythritol triacrylate.

    abstract::High-molecular-weight (co)polymers of trimethylene carbonate and D,L-lactide are efficiently crosslinked using PETA during gamma irradiation. Form-stable networks with gel contents of 86 ± 5 to 96 ± 1 are obtained from non-crystalline (co)polymers. Glass transition temperatures and elastic moduli of the networks can b...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Bat E,van Kooten TG,Feijen J,Grijpma DW

    更新日期:2011-07-07 00:00:00

  • Triggered drug release from dynamic microspheres via a protein conformational change.

    abstract::In this study we formed and characterized dynamic hydrogel microspheres in which a protein conformational change was used to control microsphere volume changes and the release of an encapsulated drug. In particular, a specific biochemical ligand, trifluoperazine, induced calmodulin's nanometer scale conformation chang...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: King WJ,Pytel NJ,Ng K,Murphy WL

    更新日期:2010-06-11 00:00:00

  • Biological natural retting for determining the hierarchical structuration of banana fibers.

    abstract::Extraction processes of natural fibers can be performed by different procedures that include mechanical, chemical and biological methods. Each method presents different advantages or drawbacks according to the amount of fiber produced or the quality and properties of fiber bundles obtained. In this study, biological n...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Gañán P,Zuluaga R,Velez JM,Mondragon I

    更新日期:2004-10-20 00:00:00

  • A comparison of thiolated and disulfide-crosslinked polyethylenimine for nonviral gene delivery.

    abstract::Branched polyethylenimine (25 kDa) is thiolated and compared with redox-sensitive crosslinked derivatives. Both polymers thiol contents are assessed; the thiolated polymers have 390-2300 mmol SH groups/mol, whereas the crosslinked polymers have lower thiol contents. Cytotoxicity assays show that both modified polymers...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Aravindan L,Bicknell KA,Brooks G,Khutoryanskiy VV,Williams AC

    更新日期:2013-09-01 00:00:00

  • Reversible Bioconjugation: Biodegradable Poly(phosphate)-Protein Conjugates.

    abstract::Protein-polymer conjugates are widely used to improve the pharmacokinetic properties of therapeutic proteins. Commercially available conjugates employ poly(ethylene glycol) (PEG) as the protective polymer; however, PEG has a number of shortcomings, including non-biodegradability and immunogenicity, that call for the d...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Steinbach T,Becker G,Spiegel A,Figueiredo T,Russo D,Wurm FR

    更新日期:2017-10-01 00:00:00

  • Microspheres made of poly(epsilon-caprolactone)-based amphiphilic copolymers: potential in sustained delivery of proteins.

    abstract::Microspheres of amphiphilic multi-block poly(ester-ether)s (PEE)s and poly(ester-ether-amide)s (PEEA)s based on poly(epsilon-caprolactone) (PCL) were investigated as delivery systems for proteins. The interest was mainly focused on the effect of their molecular structure and composition on the overall properties of th...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Quaglia F,Ostacolo L,Nese G,De Rosa G,La Rotonda MI,Palumbo R,Maglio G

    更新日期:2005-10-20 00:00:00

  • Study on Self-Assembled Well-Defined PEG Graft Copolymers as Efficient Drug-Loaded Nanoparticles for Anti-Inflammatory Therapy.

    abstract::The core-shell micelles in aqueous solutions were prepared at low CAC values (<0.1 mg · mL(-1) ) from amphiphilic graft copolymers consisting of poly(methyl methacrylate) backbone (120-165 units) with loosely grafted (17-40%) hydroxyl-capped PEG (9 vs 6 units in side chain) for drug loading. Hydrophobic indomethacin (...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Maksym-Bębenek P,Neugebauer D

    更新日期:2015-11-01 00:00:00

  • Cytocompatible, Photoreversible, and Self-Healing Hydrogels for Regulating Bone Marrow Stromal Cell Differentiation.

    abstract::Photo-crosslinking and self-healing have received considerable attention for the design of intelligent materials. A novel photostimulated, self-healing, and cytocompatible hydrogel system is reported. A coumarin methacrylate crosslinker is synthesized to modify the polyacrylamide-based hydrogels. With the [2+2] cyclo-...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Yu L,Xu K,Ge L,Wan W,Darabi A,Xing M,Zhong W

    更新日期:2016-09-01 00:00:00

  • Amides and Heparin-Like Polymer Co-Functionalized Graphene Oxide Based Core @ Polyethersulfone Based Shell Beads for Bilirubin Adsorption.

    abstract::Excessive bilirubin in the body of patient with liver dysfunction or metabolic obstruction may cause jaundice with irreversible brain damage, and new type of adsorbent for bilirubin is under frequent investigation. Herein, graphene oxide based core @ polyethersulfone-based shell beads are fabricated by phase inversion...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: He C,Li M,Zhang J,Yan B,Zhao W,Sun S,Zhao C

    更新日期:2020-08-01 00:00:00

  • Mucin macromolecules in normal, adenomatous, and carcinomatous colon: evidence for the neotransformation.

    abstract::Mucins are high molecular-weight glycoproteins having oligosaccharides attached to serine or threonine residues of the mucin core protein backbone by O-glycosidic linkages. They are major components of mucus, covering the luminal surfaces of epithelial respiratory, gastrointestinal and reproductive tracts, and respons...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章,评审


    authors: Aksoy N,Akinci OF

    更新日期:2004-05-17 00:00:00

  • Development, Validation, and Performance of Chitosan-Based Coatings Using Catechol Coupling.

    abstract::The use of long-lasting polymer coatings on biodevice surfaces has been investigated to improve material-tissue interaction, minimize adverse effects, and enhance their functionality. Natural polymers, especially chitosan, are of particular interest due to their excellent biological properties, such as biocompatibilit...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Souza Campelo C,Chevallier P,Loy C,Silveira Vieira R,Mantovani D

    更新日期:2020-01-01 00:00:00

  • Random and sequential copolypeptides containing O-phospho-L-threonine and L-aspartic acid; roles in CaCO3 biomineralization.

    abstract::The present study describes the synthesis of novel polypeptides containing O-phospho-L-threonine [Thr(PO(3)H(2))] and L-aspartic acid. Random copolypeptides copoly[Thr(PO(3)H(2))(X)Asp(Y)] (X:Y = 25:75, 50:50, 75:25), were conventionally prepared by copolymerization of Thr(PO(3)Ph(2)) N-carboxyanhydride (NCA) and Asp(...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Hayashi S,Ohkawa K,Yamamoto H

    更新日期:2006-03-14 00:00:00

  • Electrogenic Single-Species Biocomposites as Anodes for Microbial Fuel Cells.

    abstract::Integration of electrogenic microorganisms remains a challenge in biofuel cell technology. Here, synthetic biocomposites ("artificial biofilms") are proposed. Bacteria (Shewanella oneidensis) are embedded in a hydrogel matrix (poly(vinyl alcohol)) via wet- and electrospinning, creating fibers and nonwoven gauzes. The ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Kaiser P,Reich S,Leykam D,Willert-Porada M,Greiner A,Freitag R

    更新日期:2017-07-01 00:00:00

  • Merging organic and polymer chemistries to create glycomaterials for glycomics applications.

    abstract::[Image: see text] Oligosaccharides at cell surfaces are known to play a critical role in many biological processes such as biorecognition, interactions between cells and with artificial surfaces, immune response, infection and inflammation. In order to facilitate studies of the role of sugars, an increasing number of ...

    journal_title:Macromolecular bioscience

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    authors: Coullerez G,Seeberger PH,Textor M

    更新日期:2006-08-07 00:00:00

  • Surface structural investigation of starch-based biomaterials.

    abstract::Surface structural characterisation of three different starch-based blends (with poly[ethylene-co-(vinyl alcohol)], cellulose acetate and polycaprolactone) was carried out. The results show that there is a difference between the bulk and the surface composition of all studied blends. Two different hypotheses were inve...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Pashkuleva I,Azevedo HS,Reis RL

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  • In vitro and in vivo evaluation of docetaxel loaded biodegradable polymersomes.

    abstract::Formulation of docetaxel (DOC), a hydrophobic anticancer drug, was successfully achieved in poly(gamma-benzyl L-glutamate)-block-hyaluronan polymersomes using a simple and reproducible nanoprecipitation method. The prepared DOC loaded polymersomes (PolyDOC) was stable either in solution or in a lyophilized form, and s...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Upadhyay KK,Bhatt AN,Castro E,Mishra AK,Chuttani K,Dwarakanath BS,Schatz C,Le Meins JF,Misra A,Lecommandoux S

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  • Multiple-Level Porous Polymer Monoliths with Interconnected Cellular Topology Prepared by Combining Hard Sphere and Emulsion Templating for Use in Bone Tissue Engineering.

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    journal_title:Macromolecular bioscience

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    authors: Paljevac M,Gradišnik L,Lipovšek S,Maver U,Kotek J,Krajnc P

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  • Preparation and properties of novel hydrogels from oxidized konjac glucomannan cross-linked chitosan for in vitro drug delivery.

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    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Yu H,Lu J,Xiao C

    更新日期:2007-09-11 00:00:00

  • Stabilization of gelatin nanoparticles without crosslinking.

    abstract::Gelatin nanoparticles can be stabilized by entrapping them in Eudragit E100 nanospheres. The size of the nanospheres was dependent on the homogenization speed, producing nanospheres of 433 nm (8000 rpm) and 176 nm (15000 rpm). The nanoparticle morphology was critically dependent on Eudragit E100 concentration; with 2%...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Khan SA,Schneider M

    更新日期:2014-11-01 00:00:00

  • Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model.

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    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Shah H,Tariq I,Engelhardt K,Bakowsky U,Pinnapireddy SR

    更新日期:2020-12-01 00:00:00

  • Nanofiber formation of hydroxylpropylcellulose (HPC).

    abstract::The aggregative behaviors of hydroxypropylcellulose (HPC) molecules in aqueous solution and on substrates have been observed by employing laser light scattering (LLS) and, after deposition on a mica surface, atomic force microscopy (AFM). LLS studies showed that the HPC molecules formed large aggregates through self-a...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Yan L,Lin W,Bangal PR

    更新日期:2006-07-14 00:00:00

  • Biocompatible fibrous networks of cellulose nanofibres and collagen crosslinked using genipin: potential as artificial ligament/tendons.

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    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Mathew AP,Oksman K,Pierron D,Harmand MF

    更新日期:2013-03-01 00:00:00

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    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Cavalieri F,Chiessi E,Finelli I,Natali F,Paradossi G,Telling MF

    更新日期:2006-08-07 00:00:00

  • Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression.

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    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Albuquerque LJC,Alavarse AC,Carlan da Silva MC,Zilse MS,Barth MT,Bellettini IC,Giacomelli FC

    更新日期:2018-02-01 00:00:00

  • Effect of Terminal Modification on the Molecular Assembly and Mechanical Properties of Protein-Based Block Copolymers.

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    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Jacobsen MM,Tokareva OS,Ebrahimi D,Huang W,Ling S,Dinjaski N,Li D,Simon M,Staii C,Buehler MJ,Kaplan DL,Wong JY

    更新日期:2017-09-01 00:00:00

  • DNA polyplexes formed using PEGylated biodegradable hyperbranched polymers.

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    journal_title:Macromolecular bioscience

    pub_type: 杂志文章


    authors: Tao L,Chou WC,Tan BH,Davis TP

    更新日期:2010-06-11 00:00:00