Abstract:
:Calcium is a ubiquitous intracellular messenger that has a crucial role in determining the proliferation, differentiation, and functions of multipotent mesenchymal stem cells (MSCs). Our study is aimed at elucidating the influence of genetically manipulating Ca2+ release-activated Ca2+ (CRAC) channel-mediated intercellular Ca2+ signaling on the multipotency of MSCs. The abilities of genetically engineered MSCs, including CRAC-overexpressing and CRAC-knockout MSCs, to differentiate into multiple mesenchymal lineages, including adipogenic, osteogenic, and chondrogenic lineages, were evaluated. CRAC channel-mediated Ca2+ influx into these cells was regulated, and the differentiation fate of MSCs was modified. Upregulation of intracellular Ca2+ signals attenuated the adipogenic differentiation ability and slightly increased the osteogenic differentiation potency of MSCs, whereas downregulation of CRACM1 expression promoted chondrogenic differentiation potency. The findings demonstrated the effects of genetically manipulating MSCs by targeting CRACM1. CRAC-modified MSCs had distinct differentiation fates to adipocytes, osteoblasts, and chondrocytes. To aid in the clinical implementation of tissue engineering strategies for joint regeneration, these data may allow us to identify prospective factors for effective treatments and could maximize the therapeutic potential of MSC-based transplantation.
journal_name
J Immunol Resjournal_title
Journal of immunology researchauthors
Liu S,Takahashi M,Kiyoi T,Toyama K,Mogi Mdoi
10.1155/2019/7510214subject
Has Abstractpub_date
2019-02-17 00:00:00pages
7510214eissn
2314-8861issn
2314-7156journal_volume
2019pub_type
杂志文章abstract:Background:Kawasaki disease (KD) is a systemic form of self-limited vasculitis in children less than five years old, and the main complication is coronary artery injury. However, the etiology of KD remains unclear. The IL-1B polymorphisms rs16944 GG and rs1143627 AA and their diplotype GA/GA have been associated with s...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2019/4730507
更新日期:2019-04-09 00:00:00
abstract::Antiphospholipid syndrome (APS) is characterized by development of venous and/or arterial thrombosis and pregnancy morbidity. Biological criteria are the persistent presence of lupus anticoagulant (LA) and/or anti-cardiolipin (aCL) and/or anti-B2GP1 autoantibodies' positivity. The assays' performances are of crucial i...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/983094
更新日期:2015-01-01 00:00:00
abstract::Bone diseases are associated with great morbidity; thus, the understanding of the mechanisms leading to their development represents a great challenge to improve bone health. Recent reports suggest that a large number of molecules produced by immune cells affect bone cell activity. However, the mechanisms are incomple...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2015/108451
更新日期:2015-01-01 00:00:00
abstract::Malaria continues being a high-impact disease regarding public health worldwide; the WHO report for malaria in 2018 estimated that ~219 million cases occurred in 2017, mostly caused by the parasite Plasmodium falciparum. The disease cost the lives of more than 400,000 people, mainly in Africa. In spite of great effort...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2019/3832513
更新日期:2019-12-30 00:00:00
abstract::To examine human gene expression during uncomplicated P. falciparum malaria, we obtained three samples (acute illness, treatment, and recovery) from 10 subjects and utilized each subject's recovery sample as their baseline. At the time of acute illness (day 1), subjects had upregulation of innate immune response, cyto...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/162639
更新日期:2015-01-01 00:00:00
abstract::Latent tuberculosis infection (LTBI) is evidence of immunological control of tuberculosis. Dormancy survival regulator (DosR) regulon-encoded proteins may have a role in the maintenance of LTBI. T cell responses to Rv1733c, Rv0081, Rv1735c, and Rv1737c DosR regulon-encoded proteins were found to be most frequent among...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2017/1593143
更新日期:2017-01-01 00:00:00
abstract::Chloroquine (CQ) and hydroxychloroquine (HCQ) are derivatives of 4-aminoquinoline compounds with over 60 years of safe clinical usage. CQ and HCQ are able to inhibit the production of cytokines such as interleukin- (IL-) 1, IL-2, IL-6, IL-17, and IL-22. Also, CQ and HCQ inhibit the production of interferon- (IFN-) α a...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2020/4582612
更新日期:2020-09-25 00:00:00
abstract::Accumulating evidence has pointed out that metastasis is the leading cause of death in several malignant tumor, including CRC. During CRC, metastatic capacity is closely correlated with reprogrammed energy metabolism. Mitochondrial Pyruvate Carrier 1 (MPC1), as the carrier of transporting pyruvate into mitochondria, l...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/8340329
更新日期:2020-08-19 00:00:00
abstract::Propionibacterium acnes (P. acnes) is a gram-positive anaerobic bacillus present in normal human skin microbiota, which exerts important immunomodulatory effects, when used as heat- or phenol-killed suspensions. We previously demonstrated that heat-killed P. acnes or its soluble polysaccharide (PS), extracted from the...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/132083
更新日期:2015-01-01 00:00:00
abstract::The age-specific reference values for immunoglobulin (Ig) serum concentrations in European bison (Bison bonasus) are lacking. Identification of immune alterations that accompany normal physiological aging will help assist in development of better monitoring health programs. In the present study, the age-associated cha...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/2614317
更新日期:2020-01-23 00:00:00
abstract::C-type lectin receptors (CLRs) on antigen-presenting cells (APCs) facilitate uptake of carbohydrate antigens for antigen presentation, modulating the immune response in infection, homeostasis, autoimmunity, allergy, and cancer. In this review, we focus on the role of the macrophage galactose type C-type lectin (MGL) i...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2015/450695
更新日期:2015-01-01 00:00:00
abstract:PURPOSES:To investigate the possible changes in B cell subsets and in B cell expression patterns of lipid rafts (LRs) and F-actin in patients with SLE and whether leflunomide treatment may have effect on these changes. METHODS:The B cell subsets and LRs expression were determined by flow cytometry and confocal microsc...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/832916
更新日期:2015-01-01 00:00:00
abstract::Easy access to a vast collection of experimental data on immune epitopes can greatly facilitate the development of therapeutics and vaccines. The Immune Epitope Database and Analysis Resource (IEDB) was developed to provide such a resource as a free service to the biomedical research community. The IEDB contains epito...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2017/5974574
更新日期:2017-01-01 00:00:00
abstract::Epidemiological studies have demonstrated that interleukin-10 (IL-10) polymorphisms may be associated with the development of Behcet's disease (BD). However, the published results were inconsistent. Therefore, this meta-analysis was conducted to derive a more precise relationship between IL-10 polymorphisms and BD sus...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/9382609
更新日期:2020-06-19 00:00:00
abstract::Reports suggesting a pathogenic role of autoantibodies directed against glutamic acid decarboxylase 65 (GAD65Abs) in cerebellar ataxias (CAs) are reviewed, and debatable issues such as internalization of antibodies by neurons and roles of epitopes are discussed. GAD65 is one of two enzymes that catalyze the conversion...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/2913297
更新日期:2017-01-01 00:00:00
abstract::Oropouche virus (OROV) is an emerging pathogen which causes Oropouche fever and meningitis in humans. Several outbreaks of OROV in South America, especially in Brazil, have changed its status as an emerging disease, but no vaccine or specific drug target is available yet. Our approach was to identify the epitope-based...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/6718083
更新日期:2018-10-08 00:00:00
abstract::Mast cells are unique tissue-resident immune cells that secrete a diverse array of biologically active compounds that can stimulate, modulate, or suppress the immune response. Although mounting evidence supports that mast cells are consistently infiltrating tumors, their role as either a driving or an opposite force f...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2018/2584243
更新日期:2018-02-05 00:00:00
abstract::Background. Systemic lupus erythematosus (SLE) is an autoimmune disease with great heterogeneity in pathogenesis and clinical symptoms. Rheumatoid factor (RF) is one key indicator for rheumatoid arthritis (RA) while immunoglobulin E (IgE) is associated with type I hypersensitivity. To better categorize SLE subtypes, w...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2017/5096741
更新日期:2017-01-01 00:00:00
abstract:Objective:Systemic lupus erythematosus (SLE) is an autoimmune systemic disease and its pathogenesis has not yet been completely clarified. Patients with SLE show a deranged lipid metabolism, which can contribute to the immunopathogenesis of the disease and to the accelerated atherosclerosis. Resolvin D1 (RvD1), a produ...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/5264195
更新日期:2018-10-21 00:00:00
abstract::Bone is sensitive to overactive immune responses, which initiate the onset of inflammatory bone disorders, such as rheumatoid arthritis and periodontitis, resulting in a significant systemic inflammatory response. On the other hand, neuroinflammation is strongly implicated in Alzheimer's disease (AD), which can be enh...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2015/471342
更新日期:2015-01-01 00:00:00
abstract::The phenotype and functional properties of antigen-presenting cells (APC), that is, circulating monocytes and generated in vitro macrophages and dendritic cells, were investigated in the patients with pulmonary tuberculosis (TB) differing in lymphocyte reactivity to M. tuberculosis antigens (PPD-reactive versus PPD-an...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/793292
更新日期:2015-01-01 00:00:00
abstract::In vitro differentiation of mesenchymal stromal cells (MSC) into osteocytes (human differentiated osteogenic cells, hDOC) before implantation has been proposed to optimize bone regeneration. However, a deep characterization of the immunological properties of DOC, including their effect on dendritic cell (DC) function,...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/526195
更新日期:2015-01-01 00:00:00
abstract::This work is aimed at investigating the effect of melittin on identified key genes in bladder cancer (BC) and further providing a theoretical basis for BC treatment. GSE35014 downloaded from the Gene Expression Omnibus (GEO) database was used to screen differentially expressed genes (DEGs) in BC cells and control. Res...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/5038172
更新日期:2018-05-03 00:00:00
abstract:Background and Aim:The presumed role of the inhibitory receptor LAIR-1 (CD305) in the inflammatory response suggests that it might contribute to the pathophysiology of chronic inflammatory diseases such as liver cirrhosis. We studied the LAIR-1 expression on liver macrophages and blood monocytes related to the progress...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2019/2974753
更新日期:2019-03-24 00:00:00
abstract::The human leukocyte antigen-G (HLA-G) is considered an immune checkpoint molecule involved in tumor immune evasion. Hypoxia and the metabolic sensor hypoxia-inducible factor 1 (HIF-1) are hallmarks of metastasization, angiogenesis, and intense tumor metabolic activity. The purpose of this review was to examine origina...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/4587520
更新日期:2017-01-01 00:00:00
abstract::Vaccination against human papillomavirus (HPV) has been progressively implemented in most developed countries for approximately 10 years. In order to increase the protection of the vaccines, a 9-valent vaccine (HPV9) was developed, which provides protection against nine types of the virus. Studies evaluating its safet...
journal_title:Journal of immunology research
pub_type: 杂志文章,meta分析,评审
doi:10.1155/2017/3736201
更新日期:2017-01-01 00:00:00
abstract::Purified protein vaccines often require adjuvants for efficient stimulation of immune responses. There is no licensed mucosal adjuvant on the market to adequately boost the immune response to purified antigens for intranasal applications in humans. Bacterial outer membrane vesicles (OMV) are attractive candidates pote...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2016/3576028
更新日期:2016-01-01 00:00:00
abstract::A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimula...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2014/232659
更新日期:2014-01-01 00:00:00
abstract::Adverse drug reactions (ADRs) remain a common and major problem in healthcare. Severe cutaneous adverse drug reactions (SCARs), such as Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN) with mortality rate ranges from 10% to more than 30%, can be life threatening. A number of recent studies demonstrated ...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/3186328
更新日期:2017-01-01 00:00:00
abstract::Background. U65, a self-aggregating peptide scaffold, traps fused protein antigens in yeast cells. Conversion to Yeast Cell Particle (YCP) vaccines by partial removal of surface mannoproteins exposes β-glucan, mediating efficient uptake by antigen-presenting cells (APCs). YCP vaccines are inexpensive, capable of rapid...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2016/2743292
更新日期:2016-01-01 00:00:00