Abstract:
:Background. U65, a self-aggregating peptide scaffold, traps fused protein antigens in yeast cells. Conversion to Yeast Cell Particle (YCP) vaccines by partial removal of surface mannoproteins exposes β-glucan, mediating efficient uptake by antigen-presenting cells (APCs). YCP vaccines are inexpensive, capable of rapid large-scale production and have potential for both parenteral and oral use. Results. YCP processing by alkaline hydrolysis exposes up to 20% of the glucan but converts scaffolded antigen and internal yeast proteins into a common aggregate, preventing selective yeast protein removal. For U65-green fluorescent protein (GFP) or U65-Apolipoprotein A1 (ApoA1) subcutaneous vaccines, maximal IgG responses in mice required 10% glucan exposure. IgG responses to yeast proteins were 5-fold lower. Proteolytic mannoprotein removal produced YCPs with only 6% glucan exposure, insufficiently porous for selective removal of even native yeast proteins. Vaccine efficacy was reduced 10-fold. Current YCP formulations, therefore, are not suitable for human use but have considerable potential for use in feed animal vaccines. Significantly, a YCP vaccine expressing a GFP fusion to VP1, the murine polyoma virus major capsid protein, after either oral or subcutaneous administration, protected mice against an intraperitoneal polyoma virus challenge, reducing viral DNA levels in spleen and liver by >98%.
journal_name
J Immunol Resjournal_title
Journal of immunology researchauthors
Tipper DJ,Szomolanyi-Tsuda Edoi
10.1155/2016/2743292subject
Has Abstractpub_date
2016-01-01 00:00:00pages
2743292eissn
2314-8861issn
2314-7156journal_volume
2016pub_type
杂志文章abstract::Human respiratory syncytial virus (RSV) is a common and highly contagious viral agent responsible for acute lower respiratory infection in infants. This pathology characterized by mucus hypersecretion and a disturbed T cell immune response is one of the major causes of infant hospitalization for severe bronchiolitis. ...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/8734504
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:To investigate the expression and clinical significance of trans-membrane MerTK (mMer) on circulating CD14+ monocytes/macrophages and soluble MerTK (sMer) levels in plasma in systemic lupus erythematosus (SLE). METHOD:108 SLE patients and 42 healthy controls were recruited in this study. The expression of mM...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2014/431896
更新日期:2014-01-01 00:00:00
abstract::The predominant distribution of γ δ T cells in the mucosal and epithelial tissues makes these unconventional lymphocytes the "guards" to contact external environment (like allergens) and to contribute to immune surveillance, as well as "vanguards" to participate in initiating mucosal inflammation. Therefore, γ δ T cel...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2014/963484
更新日期:2014-01-01 00:00:00
abstract::Bacterial vaginosis (BV), the most common genital infection in reproductive-aged women, is associated with increased risk of sexually transmitted infections. Its etiology remains unclear, especially the role of Gardnerella (G.) vaginalis, an anaerobic bacterium characteristic of the BV-alteration of the vaginal ecosys...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2016/9747480
更新日期:2016-01-01 00:00:00
abstract::Antiphospholipid syndrome (APS) is characterized by development of venous and/or arterial thrombosis and pregnancy morbidity. Biological criteria are the persistent presence of lupus anticoagulant (LA) and/or anti-cardiolipin (aCL) and/or anti-B2GP1 autoantibodies' positivity. The assays' performances are of crucial i...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/983094
更新日期:2015-01-01 00:00:00
abstract::Phagocytosis of zymosan by phagocytes is a widely used model of microbial recognition by the innate immune system. Live-cell imaging showed that fluorescent protein-fused Rab35 accumulated in the membranes of phagocytic cups and then dissociated from the membranes of newly formed phagosomes. By our novel pull-down ass...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/429439
更新日期:2015-01-01 00:00:00
abstract:Objective:Systemic lupus erythematosus (SLE) is an autoimmune systemic disease and its pathogenesis has not yet been completely clarified. Patients with SLE show a deranged lipid metabolism, which can contribute to the immunopathogenesis of the disease and to the accelerated atherosclerosis. Resolvin D1 (RvD1), a produ...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/5264195
更新日期:2018-10-21 00:00:00
abstract::Group A streptococcus (GAS) is known to cause a broad spectrum of illness, from pharyngitis and impetigo, to autoimmune sequelae such as rheumatic heart disease, and invasive diseases. It is a significant cause of infectious disease morbidity and mortality worldwide, but no efficacious vaccine is currently available. ...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2015/167089
更新日期:2015-01-01 00:00:00
abstract:Objectives:Both thymic stromal lymphopoietin (TSLP) and dendritic cells (DCs) are involved in many autoimmune diseases, but the potential roles of TSLP and DCs in bullous pemphigoid (BP) have not been clarified. We sought to explore the contributions of TSLP and DCs in patients with BP. Methods:TSLP levels in sera and...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/4594630
更新日期:2020-09-24 00:00:00
abstract:Background:Monocytic myeloid-derived suppressor cells (M-MDSCs) characterized with the phenotype of CD14+HLA-DRlow/- have attracted a lot of attention in the field of human tumor immunology. However, little is known about the roles of M-MDSCs in acute myeloid leukemia (AML) as opposed to their multiple roles in solid t...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/7363084
更新日期:2020-12-21 00:00:00
abstract::Rheumatoid arthritis (RA) is an inflammatory autoimmune disorder; abnormal T cell immunity plays a critical role in the development of RA. Recently, A20 was identified as a key negative regulator for T cell activation and inflammatory signaling and may be involved in RA pathogenesis. In this study, we analyzed the exp...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2014/492872
更新日期:2014-01-01 00:00:00
abstract:Objective:This study aimed to investigate the correlation of CD4+/PD-1+ or CD4+/PD-1- tumor-infiltrating lymphocytes with pathological characteristics in breast cancer patients. Methods:A cross-sectional study consecutively recruited 133 patients with invasive ductal breast cancer. The expression of CD4, programmed ce...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/5690258
更新日期:2018-07-04 00:00:00
abstract::An important function of the immune system is its ability to differentiate between healthy cells in the organism and "foreign" cells, allowing the latest to be attacked and the first ones to be conserved. The most important molecules in this process are considered to be checkpoint inhibitors. This review is focused on...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2018/4625472
更新日期:2018-10-17 00:00:00
abstract::Propionibacterium acnes (P. acnes) is a gram-positive anaerobic bacillus present in normal human skin microbiota, which exerts important immunomodulatory effects, when used as heat- or phenol-killed suspensions. We previously demonstrated that heat-killed P. acnes or its soluble polysaccharide (PS), extracted from the...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/132083
更新日期:2015-01-01 00:00:00
abstract::T follicular helper cells (TFH) are a subset of recently discovered CD4+ T cells. Their major function is to participate in the formation of germinal centres (GCs) and promote B cell proliferation and differentiation to play important roles in the production of antibodies. Currently, the functions of TFH cells are cle...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2018/7281453
更新日期:2018-07-02 00:00:00
abstract::Macrophages, a kind of innate immune cells, derive from monocytes in circulation and play a crucial role in the innate and adaptive immunity. Under the stimulation of the signals from local microenvironment, macrophages generally tend to differentiate into two main functional phenotypes depending on their high plastic...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2020/8148272
更新日期:2020-07-28 00:00:00
abstract::The present study was aimed at determining the activity of aqueous and methanolic extracts of Tinospora cordifolia (AETC and METC) against Salmonella typhimurium. In vitro anti-Salmonella activity of T. cordifolia was determined through the broth dilution and agar well diffusion assays. The immune-stimulating potentia...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2017/1787803
更新日期:2017-01-01 00:00:00
abstract::TACI is a membrane receptor of BAFF and APRIL, contributing to the differentiation and survival of normal B cells. Although malignant B cells are also subjected on TACI signaling, there is a remarkable intradisease and interindividual variability of TACI expression in B-cell malignancies. The aim of our study was to e...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/478753
更新日期:2015-01-01 00:00:00
abstract::Endotoxin shock is a life-threatening disorder, associated with the rapid release of neutrophil enzymes, including neutrophil collagenase/matrix metalloproteinase-8 (MMP-8) and gelatinase B/matrix metalloproteinase-9 (MMP-9). After activation, these enzymes cleave extracellular matrix components and cytokines and thus...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2014/747426
更新日期:2014-01-01 00:00:00
abstract::Many immune-based intestinal disorders, such as ulcerative colitis and Crohn's disease, as well as other illnesses, may have the intestines as an initial cause or aggravator in the development of diseases, even apparently not correlating directly to the intestine. Diabetes, obesity, multiple sclerosis, depression, and...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2015/931574
更新日期:2015-01-01 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDAC) remains one of the most devastating cancer types despite the improvement of modern medicine. In our present study, we found that dickkopf-related protein 2 (DKK2) shares a higher expression in PDAC compared with adjacent pancreas tissue in tissue microarray. In addition, an elev...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2019/8656282
更新日期:2019-09-08 00:00:00
abstract::Bronchopulmonary dysplasia (BPD) is a severe complication of the respiratory system associated with preterm birth. Type 2 innate lymphoid cells (ILC2s) play a major role in tissue homeostasis, inflammation, and wound healing. However, the role in BPD remains unclear. The present study showed that ILC2s, interleukin-4 ...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/8050186
更新日期:2020-10-31 00:00:00
abstract::Regulatory T-cells (Tregs) are a very important subtype of lymphocytes when it comes to self-control in the human immunological system. Tregs are decisive not only in the protection against destruction of own tissues by autoimmune immunocompetent cells but also in the immunological answer to developing cancers. On the...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2019/2816498
更新日期:2019-03-03 00:00:00
abstract::Bone is sensitive to overactive immune responses, which initiate the onset of inflammatory bone disorders, such as rheumatoid arthritis and periodontitis, resulting in a significant systemic inflammatory response. On the other hand, neuroinflammation is strongly implicated in Alzheimer's disease (AD), which can be enh...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2015/471342
更新日期:2015-01-01 00:00:00
abstract::Recruitment of human NK cells to porcine tissues has been demonstrated in pig organs perfused ex vivo with human blood in the early 1990s. Subsequently, the molecular mechanisms leading to adhesion and cytotoxicity in human NK cell-porcine endothelial cell (pEC) interactions have been elucidated in vitro to identify t...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/4627384
更新日期:2017-01-01 00:00:00
abstract::Adverse drug reactions (ADRs) remain a common and major problem in healthcare. Severe cutaneous adverse drug reactions (SCARs), such as Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN) with mortality rate ranges from 10% to more than 30%, can be life threatening. A number of recent studies demonstrated ...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/3186328
更新日期:2017-01-01 00:00:00
abstract::Primary Sjogren's syndrome (pSS) is a chronic autoimmune disease. The aim of the study was to establish whether in patients with pSS without central nervous system (CNS) involvement, the function of the central portion of the sensory pathway can be challenged. In 33 patients with pSS without clinical features of CNS d...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/8174340
更新日期:2018-04-05 00:00:00
abstract::Schmallenberg virus (SBV), discovered in continental Europe in late 2011, causes mild clinical signs in adult ruminants, including diarrhoea and reduced milk yield. However, fetal infection can lead to severe malformation in newborn offspring. To develop improved reagents for SBV serology, a high-level yeast expressio...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2014/160316
更新日期:2014-01-01 00:00:00
abstract::Background. Millions of people are infected with hepatitis C virus (HCV) worldwide and 30% spontaneously clear the infection. Reasons for HCV clearance without antiviral treatment are not well understood. Methods. Blood was collected for DNA analysis from patients with chronic HCV infection or evidence of spontaneous ...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2016/6530436
更新日期:2016-01-01 00:00:00
abstract::Cationic protein is a cytotoxic protein secreted by eosinophils and takes part in the damage of airway epithelium in asthma. Poly-L-arginine (PLA), a synthetic cationic protein, is widely used to mimic the biological function of the natural cationic protein in vitro. Previous studies demonstrated the damage of the air...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/3651743
更新日期:2018-06-14 00:00:00