Abstract：
:Accumulating evidence has pointed out that metastasis is the leading cause of death in several malignant tumor, including CRC. During CRC, metastatic capacity is closely correlated with reprogrammed energy metabolism. Mitochondrial Pyruvate Carrier 1 (MPC1), as the carrier of transporting pyruvate into mitochondria, linked the glycolysis and TCA cycle, which would affect the energy production. However, the specific role of MPC1 on tumor metastasis in CRC remains unexplored. Here, by data mining of genes involved in pyruvate metabolism using the TCGA dataset, we found that MPC1 was significantly downregulated in CRC compared to nontumor tissues. Similar MPC1 expression pattern was also found in multiple GEO datasets. IHC staining in both human sample and AOM/DSS induced mouse CRC model revealed significant downregulation of MPC1. What is more, we found that MPC1 expression was gradually decreased in normal tissue, primary CRC, and metastasis CRC. Additionally, poor prognosis emerged in the MPC1 low expression patients, especially in patients with metastasis. Following, functional tests showed that MPC1 overexpression inhibited the motility of CRC cells in vitro and MPC1 silencing enhanced liver metastases in vivo. Furthermore, we uncovered that decreased MPC1 activated the Wnt/β-catenin pathway by promoting nuclear translocation of β-catenin to mediate the expression of MMP7, E-cadherin, Snail1, and myc. Collectively, our data suggest that MPC1 has the potential to be served as a promising biomarker for diagnosis and a therapeutic target in CRC.
journal_name
J Immunol Resjournal_title
Journal of immunology researchauthors
Tian GA,Xu CJ,Zhou KX,Zhang ZG,Gu JR,Zhang XL,Wang YHdoi
10.1155/2020/8340329subject
Has Abstractpub_date
2020-08-19 00:00:00pages
8340329eissn
2314-8861issn
2314-7156journal_volume
2020pub_type
杂志文章abstract：:It has been shown that rapamycin is able to significantly increase the expression of FoxP3 and suppress activity in induced Treg (iTreg) cells in vivo and in vitro. CD39 is a newly determined Treg marker that relates to cell suppression. Runx1, a regulator of FoxP3, controls the expression of adenosine deaminase (ADA)...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2014/989434
更新日期：2014-01-01 00:00:00
abstract：:Anti-CD3 antibody has been employed for various immune-mediated disorders. However, whether anti-CD3 administration leads to rapid metabolic alternation has not been well investigated. In the current study, we studied how anti-CD3 treatment affected blood glucose levels in mice. We found that anti-CD3 treatment induce...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2014/326708
更新日期：2014-01-01 00:00:00
abstract：:Calcium is a ubiquitous intracellular messenger that has a crucial role in determining the proliferation, differentiation, and functions of multipotent mesenchymal stem cells (MSCs). Our study is aimed at elucidating the influence of genetically manipulating Ca2+ release-activated Ca2+ (CRAC) channel-mediated intercel...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2019/7510214
更新日期：2019-02-17 00:00:00
abstract：Objective:Rheumatoid arthritis (RA) multidrug resistance is associated with P-glycoprotein (P-gp) overexpression. We investigated the effects of methotrexate (MTX) alone and combined with 4-hydroperoxycyclophosphamide (4-HC) on P-gp expression in fibroblast-like synoviocytes (FLSs) from patients with RA and examined th...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2018/3619320
更新日期：2018-02-18 00:00:00
abstract：:Epstein-Barr virus is a very common human virus that infects 90% of human adults. EBV replicates in epithelial and B cells and causes infectious mononucleosis. EBV infection is also linked to various cancers, including Burkitt's lymphoma and nasopharyngeal carcinomas, and autoimmune diseases such as multiple sclerosis...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2017/9363750
更新日期：2017-01-01 00:00:00
abstract：Background:Kawasaki disease (KD) is a systemic form of self-limited vasculitis in children less than five years old, and the main complication is coronary artery injury. However, the etiology of KD remains unclear. The IL-1B polymorphisms rs16944 GG and rs1143627 AA and their diplotype GA/GA have been associated with s...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2019/4730507
更新日期：2019-04-09 00:00:00
abstract：:Although it is known that systemic form of lupus erythematosus (LE) and metabolic syndrome (MetS) are frequently observed together, there are no published reports on MetS in patients with skin-restricted LE. We aimed to compare the frequencies of MetS and its components in discoid LE (DLE) with the non-DLE control gro...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2017/3972706
更新日期：2017-01-01 00:00:00
abstract：:The present study was aimed at determining the activity of aqueous and methanolic extracts of Tinospora cordifolia (AETC and METC) against Salmonella typhimurium. In vitro anti-Salmonella activity of T. cordifolia was determined through the broth dilution and agar well diffusion assays. The immune-stimulating potentia...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2017/1787803
更新日期：2017-01-01 00:00:00
abstract：:Probiotics are commensal or nonpathogenic microbes that confer beneficial effects on the host through several mechanisms such as competitive exclusion, antibacterial effects, and modulation of immune responses. Some probiotics have been found to regulate immune responses via immune regulatory mechanisms. T regulatory ...
journal_title：Journal of immunology research
pub_type： 杂志文章,评审
doi：10.1155/2019/1603758
更新日期：2019-04-16 00:00:00
abstract：:Type I interferon (IFN-I) plays a critical role in the antitumor immune response. In our previous study, we showed that IFN-I-inducible 2'-5' oligoadenylate synthetase-like 1 (OASL1) negatively regulated IFN-I production upon tumor challenge similar to that of viral infection. Thus, OASL1-deficient (Oasl1-/-) mice wer...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2019/7596786
更新日期：2019-03-31 00:00:00
abstract：:Bullous systemic lupus erythematosus (BSLE) is an autoantibody-mediated vesiculobullous disease in patients with SLE. Autoimmunity in BSLE is characterized by the presence of circulating anti-type VII collagen antibodies. BSLE patients often present with multiple, tense, clear fluid-filled vesicles and bullae overlyin...
journal_title：Journal of immunology research
pub_type： 杂志文章,评审
doi：10.1155/2015/167064
更新日期：2015-01-01 00:00:00
abstract：:Background. Systemic lupus erythematosus (SLE) is an autoimmune disease with great heterogeneity in pathogenesis and clinical symptoms. Rheumatoid factor (RF) is one key indicator for rheumatoid arthritis (RA) while immunoglobulin E (IgE) is associated with type I hypersensitivity. To better categorize SLE subtypes, w...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2017/5096741
更新日期：2017-01-01 00:00:00
abstract：:RANKL (receptor activator of nuclear factor kappa-β ligand) and OPG (osteoprotegerin) are two proteins involved in bone remodelling. During the active phase of periodontal disease, an imbalance between the ratios of the two elements can be noticed. While the expression of RANKL is elevated compared with that of OPG, t...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2019/6195258
更新日期：2019-04-28 00:00:00
abstract：:The bioactive and anti-inflammatory role of human milk components has been recognized; active milk components include soluble forms of Toll-like receptors (TLRs). Preterm babies are more susceptible to infections and may succumb to necrotizing enterocolitis (NEC), a gastrointestinal disease which is exacerbated by an ...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2019/4078671
更新日期：2019-12-04 00:00:00
abstract：:Molecular dynamics was used to simulate large molecules of the immune system (major histocompatibility complex class I, presented epitope, T-cell receptor, and a CD8 coreceptor.) To characterize the relative orientation and movements of domains local coordinate systems (based on principal component analysis) were gene...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2015/210675
更新日期：2015-01-01 00:00:00
abstract：:Background. Millions of people are infected with hepatitis C virus (HCV) worldwide and 30% spontaneously clear the infection. Reasons for HCV clearance without antiviral treatment are not well understood. Methods. Blood was collected for DNA analysis from patients with chronic HCV infection or evidence of spontaneous ...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2016/6530436
更新日期：2016-01-01 00:00:00
abstract：:The rationale for a type 17 signature in the pathogenesis of spondyloarthritis (SpA) has been increasing and being ratified in studies recently. IL-7 is a cytokine whose ability to stimulate IL-17 production in both innate and adaptive immunity cells has made it a promising target not only for a better understanding o...
journal_title：Journal of immunology research
pub_type： 杂志文章,评审
doi：10.1155/2019/7453236
更新日期：2019-05-29 00:00:00
abstract：:Objective. It has been shown that podocyte injury represents an important pathological basis that contributes to proteinuria and eventually leads to kidney failure. High glucose (HG) activates macrophage polarization, further exacerbating HG-induced podocyte injury. Our previous study on diabetic nephropathy rats indi...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2020/8263598
更新日期：2020-08-27 00:00:00
abstract：:Antiphospholipid syndrome (APS) is characterized by development of venous and/or arterial thrombosis and pregnancy morbidity. Biological criteria are the persistent presence of lupus anticoagulant (LA) and/or anti-cardiolipin (aCL) and/or anti-B2GP1 autoantibodies' positivity. The assays' performances are of crucial i...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2015/983094
更新日期：2015-01-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is characterized by deregulated activation of T and B cells, autoantibody production, and consequent formation of immune complexes. Liposomes with nonbilayer phospholipid arrangements (NPA), induced by chlorpromazine, procainamide, or manganese, provoke a disease resembling human lup...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2017/8751642
更新日期：2017-01-01 00:00:00
abstract：PURPOSES:To investigate the possible changes in B cell subsets and in B cell expression patterns of lipid rafts (LRs) and F-actin in patients with SLE and whether leflunomide treatment may have effect on these changes. METHODS:The B cell subsets and LRs expression were determined by flow cytometry and confocal microsc...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2015/832916
更新日期：2015-01-01 00:00:00
abstract：:Malaria continues being a high-impact disease regarding public health worldwide; the WHO report for malaria in 2018 estimated that ~219 million cases occurred in 2017, mostly caused by the parasite Plasmodium falciparum. The disease cost the lives of more than 400,000 people, mainly in Africa. In spite of great effort...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2019/3832513
更新日期：2019-12-30 00:00:00
abstract：:Toll/IL-1R-domain-containing adaptor-inducing IFN-β (TRIF) is an important adaptor for TLR3- and TLR4-mediated inflammatory signaling pathways. Recent studies have shown that TRIF plays a key role in vessel inflammation and atherosclerosis; however, the precise mechanisms are unclear. We investigated the mechanisms of...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2018/6249085
更新日期：2018-06-07 00:00:00
abstract：:Amiselimod (MT-1303) is a novel and selective sphingosine 1-phosphate receptor-1 (S1P1) modulator with a more favorable cardiac safety profile than other S1P1 receptor modulators. In this study, we evaluated the effects of MT-1303 on the progression of lupus nephritis in two well-known murine systemic lupus erythemato...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2019/5821589
更新日期：2019-12-23 00:00:00
abstract：:Evidences show that purinergic signaling is involved in processes associated with health and disease, including noncommunicable, neurological, and degenerative diseases. These diseases strike from children to elderly and are generally characterized by progressive deterioration of cells, eventually leading to tissue or...
journal_title：Journal of immunology research
pub_type： 杂志文章,评审
doi：10.1155/2018/4892473
更新日期：2018-08-12 00:00:00
abstract：:Recruitment of human NK cells to porcine tissues has been demonstrated in pig organs perfused ex vivo with human blood in the early 1990s. Subsequently, the molecular mechanisms leading to adhesion and cytotoxicity in human NK cell-porcine endothelial cell (pEC) interactions have been elucidated in vitro to identify t...
journal_title：Journal of immunology research
pub_type： 杂志文章,评审
doi：10.1155/2017/4627384
更新日期：2017-01-01 00:00:00
abstract：:Rheumatoid arthritis (RA) is a disease of chronic systemic inflammation (SI). In the present study, we used four datasets to explore whether methylation-derived neutrophil-to-lymphocyte ratio (mdNLR) might be a marker of SI in new onset, untreated, and treated prevalent RA cases and/or a marker of treatment response t...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2018/2624981
更新日期：2018-08-14 00:00:00
abstract：:HLA-G has a relevant role in immune response regulation. The overall structure of the HLA-G coding region has been maintained during the evolution process, in which most of its variable sites are synonymous mutations or coincide with introns, preserving major functional HLA-G properties. The HLA-G promoter region is d...
journal_title：Journal of immunology research
pub_type： 杂志文章,评审
doi：10.1155/2014/734068
更新日期：2014-01-01 00:00:00
abstract：Background and Aim:The presumed role of the inhibitory receptor LAIR-1 (CD305) in the inflammatory response suggests that it might contribute to the pathophysiology of chronic inflammatory diseases such as liver cirrhosis. We studied the LAIR-1 expression on liver macrophages and blood monocytes related to the progress...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2019/2974753
更新日期：2019-03-24 00:00:00
abstract：:In vitro differentiation of mesenchymal stromal cells (MSC) into osteocytes (human differentiated osteogenic cells, hDOC) before implantation has been proposed to optimize bone regeneration. However, a deep characterization of the immunological properties of DOC, including their effect on dendritic cell (DC) function,...
journal_title：Journal of immunology research
pub_type： 杂志文章
doi：10.1155/2015/526195
更新日期：2015-01-01 00:00:00
