Abstract：
PURPOSE:Mevalonate pathway inhibitors are potentially useful chemotherapeutic agents showing growth inhibition and pro-apoptotic effects in cancer cells. The effects of statins and bisphosphonates on cancer growth are attributed to a reduction in protein isoprenylation. Post-translational modification and activation of GTPase binding Ras superfamily permit the recruitment of these signal proteins to membranes where they mediate the cancer phenotype. Here, the effects of three inhibitors of the mevalonate pathway and one specific inhibitor of sterol regulatory element-binding proteins were studied in both an ER-negative, Ras-inactive breast (MDA-MB-231) and lung adenocarcinoma (CaLu-1) cells in vitro. METHODS:Treated cells were subject to genome-wide gene expression profiling. A gene subset was established so that the epithelial to mesenchymal transition (EMT) could be observed and compared with signalling protein shifts. RESULTS:Within the subset, some genes normally up-regulated during EMT were asymmetrically reduced by a Δ-24 DHCR inhibitor in the lung cells. Signalling proteins associated with caveolae were down-regulated by this oxidoreductase inhibitor, while those associated with membrane rafts were up-regulated. CONCLUSIONS:This study decouples isoprenylation effects from cholesterol events per se. The data support a hypothesis that caveolae are abolished by Δ-24 DHCR intervention and it is revealed that these microdomains are vital EMT signalling structures for lung cells but not ER- and Ras-negative breast cells. When signalling by extracellular signals is quenched by removal of the hydrophilic conduit provided by caveolae, the transcriptome responds by moving the cellular identity towards quiescence.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Garnett DJdoi
10.1007/s00432-015-2074-3subject
Has Abstractpub_date
2016-03-01 00:00:00pages
611-8issue
3eissn
0171-5216issn
1432-1335pii
10.1007/s00432-015-2074-3journal_volume
142pub_type
杂志文章abstract：PURPOSE:Metastasizing epithelioid sarcoma (ES) is an extremely aggressive tumor, because conventional chemotherapy and irradiation are largely ineffective. Here, we analyzed the impact of the CD95-mediated drug-induced apoptosis in ES cell lines. METHODS:The effects of paclitaxel (Taxol) and 5-FU were determined by MT...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-007-0340-8
更新日期：2008-06-01 00:00:00
abstract：PURPOSE:Piperlongumine (PL) has been shown to selectively induce apoptotic cell death in cancer cells via reactive oxygen species (ROS) accumulation. In this study, we characterized a molecular mechanism for PL-induced cell death. METHODS:Cell viability and cell death were assessed by MTT assay and Annexin V-FITC/PI s...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-014-1777-1
更新日期：2014-12-01 00:00:00
abstract：:Metastasis remains one of the major challenges before hepatocellular carcinoma (HCC) is finally conquered. This paper summarized a decade's studies on HCC metastasis at the Liver Cancer Institute of Fudan University. We have established a stepwise metastatic human HCC model system, which included a metastatic HCC mode...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s00432-003-0511-1
更新日期：2004-04-01 00:00:00
abstract：:The hospital records of 60 female and 56 male pancreatic cancer patients and 232 age-, race-, sex-, and hospital-matched controls were reviewed for evidence of prior thyroid disease, cholecystectomy, appendectomy, alcohol consumption and among females, for diseases for the endometrium and ovary. No statistically signi...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00410309
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVES:Evidence from multiple clinical trials showed that local consolidative therapy (LCT) improved survival in oligometastatic non-small cell lung cancer (NSCLC) patients. In the present study, we aim to explore the potential role of microwave ablation (MWA) as LCT for epidermal growth factor receptor (EGFR)-muta...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-019-03043-6
更新日期：2020-01-01 00:00:00
abstract：PURPOSE:The spread of malignant lymph nodes due to malignancies of the head and neck is systematically observed. However, sentinel lymph nodes in the cervical region, such as in the axillary or supraclavicular regions, are not described. Therefore, precise preoperative lymph node screening of all neck compartments is r...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-008-0487-y
更新日期：2009-06-01 00:00:00
abstract：OBJECTIVE:Isocitrate dehydrogenase (IDH)-wildtype glioblastomas are the most malignant glial tumours. Median survival is only 14-16 months after diagnosis, with patients aged ≥ 65 years reportedly showing worse outcome. This study aimed to further evaluate the prognostic role of age in a homogenously treated patient co...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-020-03334-3
更新日期：2021-01-01 00:00:00
abstract：:Bleomycin was injected i.p. in rats, and the amount of expired ethane which indicates lipid peroxidation was followed up for 78 h. Compared to controls neither 1 x 30 mg/kg and 2 x 30 mg/kg nor 1 x 70 mg/kg bleomycin led to increased ethane expiration, although body weight loss indicated toxicity. That pulmonary toxic...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00409644
更新日期：1982-01-01 00:00:00
abstract：:To date, all risk assessment studies on benzene have been based almost exclusively on epidemiological data. We have attempted a more integrated and quantitative evaluation of carcinogenic risk for humans, trying to utilize, in addition to the epidemiological data, all data available, specifically data on metabolism, g...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00397718
更新日期：1987-01-01 00:00:00
abstract：:Protein extracted from conventional formalin-fixed and paraffin-embedded tissue sections of human gliomas was examined for immunoblot analysis using antibody against the placental form of glutathione S-transferase (GST-pi). Four benign astrocytomas, five anaplastic astrocytomas and four glioblastomas were used in this...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01215931
更新日期：1993-01-01 00:00:00
abstract：:The purpose of this study was to examine the structure/activity relationships of a series of substituted benzamides as poly(ADP-ribose) polymerase inhibitors. The experimental approach has involved the use of in vitro and in vivo assays in order to gather information either on the intrinsic activity of the benzamides ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01637083
更新日期：1990-01-01 00:00:00
abstract：:In supporting the human-tumor cloning effort of the Southwest Oncology Group, we conducted an independent retrospective study to evaluate the clinical correlations of the soft-agar colony-forming assay developed by Hamburger and Salmon (1977). This study was made with the cooperation of 76 clinicians and 11 hospitals ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 临床试验,杂志文章
doi：10.1007/BF01884250
更新日期：1985-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Treatment for malignant ascites in advanced ovarian cancer (OC) patients remains controversial. The objective of this study was to investigate the efficacy of combined continuous circulatory hyperthermic intraperitoneal chemotherapy (HIPEC) preceded or followed by cytoreductive surgery (CRS) f...
journal_title：Journal of cancer research and clinical oncology
pub_type： 临床试验,杂志文章
doi：10.1007/s00432-014-1692-5
更新日期：2014-09-01 00:00:00
abstract：PURPOSE:The feasibility of radioimmunotherapy (RAIT) combined with 5-fluorouracil (5-FU) was examined in colon cancer xenografts. The mode of interaction of the two treatments was also investigated. METHODS:Mice bearing human colon cancer were treated with a combination of 4.63 MBq (L-RAIT) or 9.25 MBq (H-RAIT) (131)I...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s004320050326
更新日期：1999-11-01 00:00:00
abstract：PURPOSE:Explore molecular monitoring patterns of patients with chronic myeloid leukemia (CML) on tyrosine kinase inhibitors therapy in China and identify variables associated with monitoring patterns. METHODS:Non-interventional, cross-sectional study using questionnaires distributed to persons with CML and answered an...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2158-8
更新日期：2016-07-01 00:00:00
abstract：:Resistance to cytotoxic chemotherapy is a major problem in the management of patients with metastatic breast cancer. Various data suggest P-glycoprotein-associated multidrug resistance (MDR) to be a relevant resistance mechanism in this tumor. The purpose of this study was to evaluate feasibility and activity of combi...
journal_title：Journal of cancer research and clinical oncology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1007/BF02351063
更新日期：1995-01-01 00:00:00
abstract：PURPOSE:Metastasis is a primary cause of colorectal cancer (CRC)-related death, and cancer stem cells (CSCs) are thought to be majorly responsible for initiating metastatic behaviors. Doublecortin-like kinase 1 (DCLK1) was recently discovered to be a marker for gastrointestinal CSCs. Here, we aimed to explore whether D...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2218-0
更新日期：2016-10-01 00:00:00
abstract：PURPOSE:The aim of this paper is to provide an overview of the most investigated optical diagnostic techniques: optical coherence tomography, fluorescence spectrometry, reflectance spectrometry, Raman spectroscopy, and confocal microscopy. METHODS:A search of three databases was conducted using specific keywords and e...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s00432-013-1423-3
更新日期：2013-07-01 00:00:00
abstract：PURPOSE:The N-myc downstream regulated gene 1 (NDRG1)/Cap43 is overexpressed in multiple cancer types, including lung cancer. In this study, we investigated the expression of NDRG1/Cap43 in lung cancer tissues and in serum from 90 lung cancer patients and 60 healthy controls. MATERIALS AND METHODS:Ninety patients with...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-012-1236-9
更新日期：2012-11-01 00:00:00
abstract：PURPOSE:The high incidence of biliary tract carcinoma in patients with anomalous pancreaticobiliary ductal junction (APBDJ) implicates that a compositional alteration in bile may contribute to the genesis of this cancer. Lysophosphatidylcholine (LPC) is generated in the bile of these patients. Given the role of cycloox...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-006-0125-5
更新日期：2006-12-01 00:00:00
abstract：PURPOSE:The introduction of immunochemotherapy has led to a significant improvement in treatment results and prognosis of diffuse large B-cell non-Hodgkins lymphoma (DLBCL) both at initial diagnosis and in relapse. Trofosfamide, an oxazaphosphorine derivative, has been utilized as alternative treatment option for patie...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-018-2772-8
更新日期：2019-01-01 00:00:00
abstract：:The objective of this study was to compare urinary metabolism of parent 3,3-dimethyl-1-phenyltriazene with that of its ring-substituted 1-(4-chlorophenyl)-3,3-dimethyltriazene and 1-(2,4,6-trichlorophenyl)-3,3-dimethyltriazene congeners, in an attempt to evaluate the molecular requirements for systemic carcinogenic ac...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00390976
更新日期：1984-01-01 00:00:00
abstract：:The responsiveness of spleen cells from C57BL/6J mice to various immunogenic stimuli was examined during the progressive growth of a poorly immunogenic fibrosarcoma T241. A strong correlation was observed between the progressive tumor growth and the depression of response to Concanavalin A, lipopolysaccharide, sheep r...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00410112
更新日期：1981-01-01 00:00:00
abstract：AIMS:This study aimed at evaluating the potential anti-proliferative effects of the microRNA let-7 family in nasopharyngeal carcinoma (NPC) cells. In addition, the association between let-7 suppression and DNA hypermethylation is examined. MATERIALS AND METHODS:Levels of mature let-7 family members (-a, -b, -d, -e, -g...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-010-0898-4
更新日期：2011-03-01 00:00:00
abstract：:The effects of three non-myelotoxic cancer drugs on the growth of neuroblastoma cells were investigated in vitro and in vivo: dihydroxyphenylalanine (L-dopa, a drug with selective toxicity for melanoma cells), DL-buthionine sulphoximine (BSO, a drug with radiosensitizing effects), and tamoxifen (a drug used in the tre...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01612766
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND:Circulating tumor cells (CTCs) have long been considered a reflection of tumor aggressiveness. Hematogenous spreading of CTCs from a primary tumor is a crucial step in the metastasis cascade, which leads ultimately to the formation of overt metastases. However, owing to the rarity of CTCs in peripheral blood...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s00432-011-0988-y
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To study the surgical outcomes of patients with a second primary lung cancer after the extrapulmonary malignancy. MATERIALS AND METHODS:Patients who underwent surgical resection for lung cancers between January 2005 and December 2014 were reviewed. Clinical data, imaging characteristics of tumors, surgical a...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-020-03310-x
更新日期：2020-12-01 00:00:00
abstract：PURPOSE:Cluster of differentiation (CD) 44 is a cell surface receptor that correlates with the development of drug resistance in epithelial ovarian cancer (EOC). Here, we prospectively investigated the clinical impact of CD44 expression on the development of chemoresistance, and on disease-free and overall survival in ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2116-5
更新日期：2016-05-01 00:00:00
abstract：PURPOSE:To study the interplay of drugs and energy metabolism of tumor cells, metabolic changes induced by chloroacetaldehyde and cytochalasin B were analyzed in colon carcinoma cells LS174T. METHODS:O(2)-consumption and extracellular acidification were recorded using a bioelectronic sensor-chip system, which monitors...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-005-0015-2
更新日期：2005-10-01 00:00:00
abstract：:Telomerase, a ribonucleoprotein enzyme, is expected to be a new marker for cancer diagnosis. TRAP (the telomeric-repeat amplification protocol) developed by Kim et al. is a sensitive method to detect telomerase activity. Telomerase activity is detected by TRAP in most malignant cells in vivo and in vitro, but it is no...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01438310
更新日期：1997-01-01 00:00:00
