Abstract:
:Telomerase, a ribonucleoprotein enzyme, is expected to be a new marker for cancer diagnosis. TRAP (the telomeric-repeat amplification protocol) developed by Kim et al. is a sensitive method to detect telomerase activity. Telomerase activity is detected by TRAP in most malignant cells in vivo and in vitro, but it is not found, or found only in very low amounts, in normal somatic cells and tissues. TRAP and its modified protocols are, however, not always suitable for measuring the activity of a large number of clinical samples to diagnose cancer, because they generally require a time-consuming detection step such as gel electrophoresis with radioactive materials. To improve the procedure for mass diagnosis, we applied a hybridization protection assay (HPA) to replace the detection step. HPA, which employs an acridinium-ester-labelled probe, is radioactivity-free, easy to handle without electrophoresis, quick, and applicable to a quantitative format. In this work we have established and demonstrated the advantages of TRAP/HPA. The telomerase activity of various primary and established cells, differentiating cancer cells, and normal and tumour colorectal and liver tissues was quantitatively analysed by TRAP/HPA. The results indicate that HPA combined with TRAP is a rapid and simple method, easy to handle and quantify, for the clinical diagnosis of cancer.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Hirose M,Abe-Hashimoto J,Ogura K,Tahara H,Ide T,Yoshimura Tdoi
10.1007/BF01438310subject
Has Abstractpub_date
1997-01-01 00:00:00pages
337-44issue
6eissn
0171-5216issn
1432-1335journal_volume
123pub_type
杂志文章abstract::Tumour necrosis factor alpha (TNF alpha) exerts cytotoxic and antiproliferative effects on neoplastic cells. It has been used as a therapeutic agent for solid tumours and haematological malignancies. We report on the ex vivo determination of the effect of recombinant human rhuTNF alpha on bone marrow aspirates by a br...
journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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更新日期:2013-05-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
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更新日期:2019-03-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/s00432-020-03160-7
更新日期:2020-06-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 社论
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journal_title:Journal of cancer research and clinical oncology
pub_type: 临床试验,杂志文章
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更新日期:2010-08-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/s00432-017-2553-9
更新日期:2018-02-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/BF00402732
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doi:10.1007/s00432-008-0520-1
更新日期:2009-06-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
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更新日期:2013-01-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
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更新日期:2010-09-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/BF01630712
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journal_title:Journal of cancer research and clinical oncology
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pub_type: 杂志文章
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/s00432-008-0529-5
更新日期:2009-07-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章,评审
doi:10.1007/s00432-014-1899-5
更新日期:2015-09-01 00:00:00