Abstract:
BACKGROUND:The CD-TK double suicide gene has become an effective therapy for bladder cancer. A novel molecular-targeted ultrasound (US) method has been developed to precisely guide nanobubbles loaded with this gene to regions within bladder tumor cells and is widely used due to its efficiency in delivering drugs to the target tumor. METHODS:Uniform nanoscaled nanobubbles loaded with CD-TK double suicide gene were developed using a thin-film hydration sonication, carbodiimide chemistry approaches, and electrostatic adsorption methods. RESULTS:In the present study, we synthesized CD-TK double suicide gene-loaded cationic nanobubbles conjugated with anti-VEGFR2 that can bind with VEGFR2-positive cells. Fluorescence and flow cytometry evidence show that CD-TK double suicide gene-loaded nanobubbles were successfully developed. CD-TK-CNBs delivered via US-mediated nanobubble destruction (UMND) enhanced transfection efficiency, overexpression of CD-TK double suicide gene, and tumor cell apoptosis, and inhibited tumor cell growth in vitro. CONCLUSIONS:These CD-TK-CNBs may become a novel treatment for bladder cancer.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Hu C,Jiang D,Wu M,Wang J,Zhang Rdoi
10.1007/s00432-020-03160-7subject
Has Abstractpub_date
2020-06-01 00:00:00pages
1415-1426issue
6eissn
0171-5216issn
1432-1335pii
10.1007/s00432-020-03160-7journal_volume
146pub_type
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