Abstract：
PURPOSE:Metastasizing epithelioid sarcoma (ES) is an extremely aggressive tumor, because conventional chemotherapy and irradiation are largely ineffective. Here, we analyzed the impact of the CD95-mediated drug-induced apoptosis in ES cell lines. METHODS:The effects of paclitaxel (Taxol) and 5-FU were determined by MTT assay. The extent of apoptosis was analyzed by light microscopy and Annexin V staining (flow cytometry). The expression of death receptors and ligands was defined by RT-PCR, Western blotting and flow cytometry. RESULTS:All cell lines expressed CD95, but not the CD95 ligand. The CD95 activation resulted in apoptosis and cell death in all cell lines. Both paclitaxel and 5-FU are able to trigger apoptosis, and furthermore, to upregulate CD95, whereas only paclitaxel increases CD95 ligand expression. Neutralizing antibodies directed against CD95 ligand effectively inhibited paclitaxel-induced cell death, thereby providing evidence for a direct involvement of the CD95 system in paclitaxel-induced apoptosis. CONCLUSIONS:Concomitant upregulation of CD95 receptor and ligand may significantly enhance the response of ES to anticancer drugs. As evident from the differential response of our clonal ES subpopulations to paclitaxel and 5-FU, effective activation of the CD95 system depends on intrinsic properties of both the chemotherapeutic agent and target cell population.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Heikaus S,Matuszek KS,Suschek CV,Ramp U,Reinecke P,Grinstein E,Haremza J,Gabbert HE,Mahotka Cdoi
10.1007/s00432-007-0340-8subject
Has Abstractpub_date
2008-06-01 00:00:00pages
689-95issue
6eissn
0171-5216issn
1432-1335journal_volume
134pub_type
杂志文章abstract：BACKGROUND:Recent advances in sequencing technologies supported the development of molecularly targeted therapy in cancer patients. Thus, genomic analyses are becoming a routine part in clinical practice and accurate detection of actionable mutations is essential to assist diagnosis and therapy choice. However, this is...
journal_title：Journal of cancer research and clinical oncology
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abstract：PURPOSE:Recent studies indicated undisputed contribution of connective tissue growth factor (CTGF) in the development of many cancers, including non-small cell lung cancer (NSCLC). However, the functional role and regulation of CTGF expression during tumorigenesis remain elusive. Our goal was to determine CTGF transcri...
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abstract：:Induced pluripotent stem (iPS) cells are a group of pluripotent stem cells artificially derived from non-pluripotent cells typically by a forced expression of specific transcription factors. Generation of cancer-specific iPS cells, also called induced pluripotent cancer (iPC) cells, provides valuable experimental plat...
journal_title：Journal of cancer research and clinical oncology
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abstract：OBJECTIVE:To evaluate through retrospective analysis the efficacy and toxicity of combination chemotherapy with etoposide, vincristine, doxorubin and dexamethasone (EVAD) as second-line therapy in patients with advanced AIDS-related Kaposi's sarcoma (AIDS-KS) after failure of first-line chemotherapy. METHODS:Eighty-ei...
journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/s00432-011-1109-7
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abstract：:Induction of gene transcription in response to extracellular stimuli constitutes an essential aspect of host defense mechanisms. Interferons (IFN) are families of cytokines that have been discovered and extensively characterized in the context of host defense against viral infections. In elucidating the mechanism of t...
journal_title：Journal of cancer research and clinical oncology
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abstract：BACKGROUND:Circulating tumor cells (CTCs) have long been considered a reflection of tumor aggressiveness. Hematogenous spreading of CTCs from a primary tumor is a crucial step in the metastasis cascade, which leads ultimately to the formation of overt metastases. However, owing to the rarity of CTCs in peripheral blood...
journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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abstract：BACKGROUND:Gastric cancer (GC) is the second most common leading cause of cancer-related death. Cancer stem cell (CSC) with the mark of CD44 played an important role in GC. rMETase was wildly exploited as chemotherapeutic option for GC. Polymers synthetic nanoparticle drug delivery systems have been commonly used for c...
journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/s00432-013-1459-4
更新日期：2013-09-01 00:00:00
abstract：PURPOSE:T cell transcription factors are nuclear effectors of the Wnt signaling transduction pathway and play crucial roles in embryonic and malignant development. Our previous study showed increased expression level of Tcf mRNA in liver cancer. In the present paper, antisense Tcf RNA was used to explore the possible t...
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journal_title：Journal of cancer research and clinical oncology
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abstract：OBJECTIVES:Tobacco exposure remains the main etiologic factor for lung cancer (LC). Interactions between environment and individual genetic profile are particularly important for this disease. The aim of this study was to evaluate the contribution of CYP1A1*2A, CYP1A1*2C, CYP2D6*4, GSTP1, GSTM1, GSTT1 and NAT2 polymorp...
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journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/s00432-002-0374-x
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/s00432-014-1777-1
更新日期：2014-12-01 00:00:00
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journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/s00432-014-1656-9
更新日期：2014-07-01 00:00:00
abstract：INTRODUCTION:Aberrant expression of RNA-binding motif protein 3 (RBM3) has been suggested as a prognostic biomarker in several malignancies. MATERIALS AND METHODS:This study was performed to analyse the prevalence and clinical significance of RBM3 immunostaining in non-small cell lung cancers (NSCLCs). Therefore, we t...
journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/s00432-019-02850-1
更新日期：2019-04-01 00:00:00
abstract：:Soft-agar clonogenicity of L1210 mouse leukemia cells and of xenografts of a human melanoma and a carcinoma of the cervix was studied sectionally by the sizes of the colonies grown under hypoxic gradients and aerobic condition. Soft-agar plating efficiency was increased in cultured L1210 cells with decreasing oxygen c...
journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/BF00402464
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journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/s00432-005-0020-5
更新日期：2005-10-01 00:00:00
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journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/BF01612771
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abstract：PURPOSE:In our previous global gene expression analysis, we identified NIPA-like domain containing 1 (NIPAL1), which encodes a magnesium transporter, as one of the most overexpressed genes in recurrent oral squamous cell carcinoma (OSCC). Although has been NIPAL1 linked with gout pathogenesis, little is known about its...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-018-2612-x
更新日期：2018-05-01 00:00:00
abstract：:Female carriers of mutations in the BRCA1 gene on chromosome 17q have a very high risk of developing breast and/or ovarian cancer during their lifetime. There is, however, little knowledge of to what extent non-genetic risk factors, such as age at menarche, age at first birth, and body mass index, alter the age at ons...
journal_title：Journal of cancer research and clinical oncology
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