Abstract:
:Metastasis remains one of the major challenges before hepatocellular carcinoma (HCC) is finally conquered. This paper summarized a decade's studies on HCC metastasis at the Liver Cancer Institute of Fudan University. We have established a stepwise metastatic human HCC model system, which included a metastatic HCC model in nude mice (LCI-D20), a HCC cell line with high metastatic potential (MHCC97), a relatively low metastatic potential cell clone (MHCC97L) and several stepwise high metastatic potential cell clones (MHCC97H, HCCLM3, and HCCLM6) from their parent MHCC97 cell. Endeavors have been made for searching human HCC metastasis-related chromosomes/proteins/genes. Monogene-based studies revealed that HCC invasion/metastasis was similar to that of other solid tumors, and the biological characteristics of small HCC were only slightly better than that of large HCC. Using comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH), genotyping, cDNA microarray, and 2-dimensional gel electrophoresis, we obtained some interesting results. In particular, in collaboration with the National Institute of Health (NIH) in the United States, we generated a molecular signature that can classify metastatic HCC patients, identified osteopontin as a lead gene in the signature, and found that genes favoring metastasis progression were initiated in the primary tumors. We also found that chromosome 8p deletion, particularly in the region of 8p23, was associated with HCC metastasis. Cytokeratin 19 was identified as one of the proteins, which was found in MHCC97H, but not in MHCC97L cells. Experimental interventions using the high metastatic nude mice model have provided clues for the prevention of HCC metastasis. Translation from workbench to bedside demonstrated that serum VEGF, microvessel density, and p53 scoring may be of value for the prediction of postoperative metastatic recurrence. Interferon alpha proved effective for the prevention of recurrence both experimentally and clinically. In conclusion, HCC metastasis that probably initiated in the primary tumor is a multigene-involved, multistep, and changing process. The further elucidation of the mechanism underlying HCC metastasis will provide a more solid basis for the prediction and prevention of the metastatic recurrence of HCC.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Tang ZY,Ye SL,Liu YK,Qin LX,Sun HC,Ye QH,Wang L,Zhou J,Qiu SJ,Li Y,Ji XN,Liu H,Xia JL,Wu ZQ,Fan J,Ma ZC,Zhou XD,Lin ZY,Liu KDdoi
10.1007/s00432-003-0511-1subject
Has Abstractpub_date
2004-04-01 00:00:00pages
187-96issue
4eissn
0171-5216issn
1432-1335journal_volume
130pub_type
杂志文章,评审abstract:PURPOSE:The TNM classification is used to assess cancers of the oral cavity, and advancements in imaging techniques have revealed clear variations in tumor volume at presentation. This study therefore aimed to clarify whether preoperative imaging, with exact measurements of the tumor, could affect post-surgery survival...
journal_title:Journal of cancer research and clinical oncology
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更新日期:2015-06-01 00:00:00
abstract:PURPOSE:Signalling through the insulin-like growth factor 1 receptor (IGF-1R) is implicated in carcinogenesis, metastasis, and resistance to cytotoxic cancer therapies. The purpose of this study was to investigate the prognostic role of IGF-1R expression in surgically resected non-small-cell lung cancer (NSCLC), and re...
journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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doi:10.1007/s00432-006-0174-9
更新日期:2007-06-01 00:00:00
abstract::A brain metastasis of bronchial squamous cell carcinoma in a patient was cultivated. The cells with epithelium-like characteristics were selected from the primary culture, and were subcultured to establish a cell line. The cultured cells were identified to be neoplastic by means of heterologous transplantation to nude...
journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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doi:10.1007/s00432-009-0735-9
更新日期:2010-06-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
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journal_title:Journal of cancer research and clinical oncology
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doi:10.1007/s00432-014-1719-y
更新日期:2014-10-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/BF00395387
更新日期:1983-01-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
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更新日期:2002-08-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
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doi:10.1007/s00432-017-2353-2
更新日期:2017-06-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/s00432-001-0301-6
更新日期:2002-01-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章,评审
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更新日期:2003-04-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 社论,评审
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/s00432-009-0723-0
更新日期:2010-06-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/BF01218524
更新日期:1995-01-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/BF00394931
更新日期:1986-01-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2020-08-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章,meta分析
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/s00432-003-0478-y
更新日期:2003-08-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
doi:10.1007/s00432-011-0980-6
更新日期:2011-07-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2015-09-01 00:00:00
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journal_title:Journal of cancer research and clinical oncology
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
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