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Assay for the determination of human carcinoma cells in circulating blood.

Abstract：

:Methods have been developed in an in vitro system (1) of assessing the number of disseminated tumor cells in peripheral blood and (2) of enriching tumor cells from peripheral blood samples for further characterization. Cells from three human carcinoma lines (E 14, ChaGo, and LEDWiDr) were mixed with leukocytes from normal individuals in various ratios. The proportions of tumor cells were determined by a quantitative assay using 3H-thymidine, 3H-leucine, and 3H-galactose incorporation. Determination of tumor cell proportions with this method was most accurate in the range of 5 X 10(4) to 5 X 10(3) tumor cells mixed with a constant number (5 X 10(5] of lymphocytes. It was possible to separate 75Se-labeled tumor cells from 51Cr-labeled blood leukocytes by centrifugation in isopyknic Percoll density gradients. These cells were mixed at different ratios and subjected to Percoll gradient centrifugation. By this approach as few as 5 X 10(3) tumor cells could by identified in the presence of 5 X 10(7) leukocytes, representing a ratio of 1: 10,000. Percoll centrifugation did not damage the tumor cells. In blood cells from two lung cancer patients with lung metastases the incorporation of 3H-thymidine and 3H-galactose was significantly enhanced compared with that in blood cells from patients with primary lung tumors and in cells from normal individuals. This difference became even more apparent when metabolically-labeled blood cells were subsequently separated by Percoll gradient centrifugation.

journal_name

J Cancer Res Clin Oncol

journal_title

Journal of cancer research and clinical oncology

authors

Schwartz R,Walk A,Toomes H,Schirrmacher V

doi

10.1007/BF00391886

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

122-9

issue

2

eissn

0171-5216

issn

1432-1335

journal_volume

109

pub_type

杂志文章

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