A gene expression profile of tumor suppressor genes commonly methylated in bladder cancer.


PURPOSE:The functional relationship between promoter hypermethylation and gene inactivation has been demonstrated for few genes only. We examined the promoter methylation status of two important tumor suppressor genes APAF-1 and DAPK-1 in bladder cancer as well as the mRNA expression pattern of these two genes for possible correlation between promoter hypermethylation and transcriptional repression. METHODS:The methylation status and mRNA expression levels were related to clinicopathological features in 34 patients with transitional cell carcinoma (TCC) of the bladder with a median clinical follow-up of more than 45 months. Tissue from ten patients with nonmalignant disease served as a control group. Quantitative real-time PCR-based detection methods were used for determination of the normalized index of methylation (NIM) as well as the mRNA expression level. RESULTS:APAF-1 and DAPK-1 methylation and mRNA expression was observed in all tumor and normal control samples investigated. Methylation (NIM) levels were significantly higher in tumor tissue for APAF-1 and DAPK-1, but median mRNA expression levels did not differ significantly comparing tumorous and non tumorous tissue. No correlation between expression levels of APAF-1 and DAPK-1 mRNA and tumor stage or grade was observed. However, in superficial TCC a strong correlation between higher NIM levels and lower mRNA expression of the APAF-1 gene was observed (P = 0.014). CONCLUSIONS:Our results, although preliminary, provide first time in vivo expression analysis of the APAF-1 gene in bladder cancer specimen, suggesting expression control by promoter methylation in early stage tumor disease of the bladder.


Christoph F,Hinz S,Kempkensteffen C,Weikert S,Krause H,Schostak M,Schrader M,Miller K




Has Abstract


2007-06-01 00:00:00












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    abstract:PURPOSE:The present study was undertaken to examine the amplification and expression status of Cks1 in breast cancer and its significance. METHODS:The amplification and expression status of Cks1 gene was examined by FISH, real-time PCR and immunohistochemistry, respectively. RNA interference was used to detect the eff...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,收录出版


    authors: Wang XC,Tian LL,Tian J,Wu HL,Meng AM

    更新日期:2009-10-01 00:00:00

  • Chemotherapy intensification in patients with advanced seminoma and adverse prognostic factors.

    abstract:PURPOSE:The aim of our study was to identify factors which influence survival in patients with disseminated seminoma in the good prognostic group according to IGCCCG, as well as to evaluate the impact of treatment intensification in patients with negative prognostic factors. METHODS:We analyzed the database of the pat...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章


    authors: Fedyanin M,Tryakin A,Bulanov A,Vybarava A,Tjulandina A,Chekini D,Sekhina O,Figurin K,Garin A,Tjulandin S

    更新日期:2015-07-01 00:00:00

  • Antitumor activity of a phenoxazine compound, 2-amino-4,4alpha-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one against human B cell and T cell lymphoblastoid cell lines: induction of mixed types of cell death, apoptosis, and necrosis.

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    journal_title:Journal of cancer research and clinical oncology

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    authors: Koshibu-Koizumi J,Akazawa M,Iwamoto T,Takasaki M,Mizuno F,Kobayashi R,Abe A,Tomoda A,Hamatake M,Ishida R

    更新日期:2002-07-01 00:00:00

  • p53 Gene status in relation to ex vivo chemosensitivity of non-small cell lung cancer.

    abstract:PURPOSE:About 40% of non-small cell lung cancer (NSCLC) patients are candidates for systemic chemotherapy, despite the fact that at diagnosis most NSCLC are usually chemoresistant both in vivo and ex vivo. It is important to develop sufficient methods of prediction of the response to chemotherapy and to find molecular ...

    journal_title:Journal of cancer research and clinical oncology

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    authors: Vogt U,Zaczek A,Klinke F,Granetzny A,Bielawski K,Falkiewicz B

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    journal_title:Journal of cancer research and clinical oncology

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    authors: Su Y,Lu J,Chen X,Liang C,Luo P,Qin C,Zhang J

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    journal_title:Journal of cancer research and clinical oncology

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    authors: Nagaoka T,Onodera H,Matsushima Y,Todate A,Shibutani M,Ogasawara H,Maekawa A

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    authors: Franzese C,Comito T,Clerici E,Di Brina L,Tomatis S,Navarria P,Reggiori G,Viganò L,Poretti D,Pedicini V,Torzilli G,Solbiati L,Scorsetti M

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    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章


    authors: Schwartz R,Walk A,Toomes H,Schirrmacher V

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    journal_title:Journal of cancer research and clinical oncology

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    authors: Herrmann E,Gerss J,Bierer S,Köpke T,Bolenz C,Hertle L,Wülfing C

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    pub_type: 临床试验,杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    authors: Tanaka T,Okamura S,Yasumoto S,Takeichi N,Kobayashi H,Niho Y

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    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章


    authors: Reitz M,Afghanyar S,Gutjahr P

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    journal_title:Journal of cancer research and clinical oncology

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