Abstract：
PURPOSE:The functional relationship between promoter hypermethylation and gene inactivation has been demonstrated for few genes only. We examined the promoter methylation status of two important tumor suppressor genes APAF-1 and DAPK-1 in bladder cancer as well as the mRNA expression pattern of these two genes for possible correlation between promoter hypermethylation and transcriptional repression. METHODS:The methylation status and mRNA expression levels were related to clinicopathological features in 34 patients with transitional cell carcinoma (TCC) of the bladder with a median clinical follow-up of more than 45 months. Tissue from ten patients with nonmalignant disease served as a control group. Quantitative real-time PCR-based detection methods were used for determination of the normalized index of methylation (NIM) as well as the mRNA expression level. RESULTS:APAF-1 and DAPK-1 methylation and mRNA expression was observed in all tumor and normal control samples investigated. Methylation (NIM) levels were significantly higher in tumor tissue for APAF-1 and DAPK-1, but median mRNA expression levels did not differ significantly comparing tumorous and non tumorous tissue. No correlation between expression levels of APAF-1 and DAPK-1 mRNA and tumor stage or grade was observed. However, in superficial TCC a strong correlation between higher NIM levels and lower mRNA expression of the APAF-1 gene was observed (P = 0.014). CONCLUSIONS:Our results, although preliminary, provide first time in vivo expression analysis of the APAF-1 gene in bladder cancer specimen, suggesting expression control by promoter methylation in early stage tumor disease of the bladder.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Christoph F,Hinz S,Kempkensteffen C,Weikert S,Krause H,Schostak M,Schrader M,Miller Kdoi
10.1007/s00432-006-0174-9subject
Has Abstractpub_date
2007-06-01 00:00:00pages
343-9issue
6eissn
0171-5216issn
1432-1335journal_volume
133pub_type
杂志文章abstract：PURPOSE:To analyse hepcidin serum and urine levels during radiotherapy for prostate cancer. METHODS:In 18 patients undergoing radiotherapy for prostate cancer, blood, plasma, and urine samples were taken before and during radiotherapy. Complete blood cell count, pro-hepcidin-, ferritin-, transferrin-, IL-1beta-, IL-6-...
journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
pub_type： 信件
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journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00393339
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/s00432-003-0423-0
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journal_title：Journal of cancer research and clinical oncology
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doi：10.1007/BF00406927
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journal_title：Journal of cancer research and clinical oncology
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更新日期：2006-02-01 00:00:00
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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journal_title：Journal of cancer research and clinical oncology
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更新日期：2014-01-01 00:00:00
