Abstract:
AIMS:Recently, the zebrafish has gained attention as an innovative experimental model to decipher molecular and cellular mechanisms involved in cardiovascular development and diseases. Nevertheless, the use of zebrafish models has been challenged because the transparency of these fish, which allows for accurate cardiac evaluation, disappears in adulthood. In this study, the epicardial outline method was performed to investigate the feasibility of echocardiography in assessing cardiac function in pathological adult zebrafish. MATERIALS AND METHODS:We attempted to estimate heart failure in adult zebrafish treated with three distinct regulators of cardiac function: phenylhydrazine hydrochloride (PHZ), doxorubicin (DOX), and ethanol. B-mode and Doppler images were evaluated at frequencies of up to 50 MHz and 40 MHz, respectively. The correlation between alterations in cardiac function, haemoglobin concentration, and myocardial histopathology were assessed. KEY FINDINGS:Cardiac output (CO) in PHZ-treated zebrafish was significantly higher than that in control zebrafish (151 ± 67 vs. 84 ± 37 μl/min, P = 0.004), whereas ejection fraction (EF) was lower (36.3 ± 10.9 vs. 50.9 ± 8.7%, P < 0.001), indicating typical high output heart failure derived from anaemia. Additionally, ventricular dysfunction in DOX-treated zebrafish was characterised by low CO (57 ± 38 μl/min) and EF (28.8 ± 10.4%), accompanied by an enlarged ventricle in diastole and systole, representing low output heart failure. For ethanol-treated zebrafish, EF was markedly reduced (39.6 ± 7.2%) indicating a dilated heart, while CO remained unchanged (90 ± 40 μl/min). SIGNIFICANCE:The epicardial outline method is an effective way of using echocardiography to assess cardiac dysfunction in pathological adult zebrafish, unlocking a major bottleneck in this research field with limited cardiac functional assays.
journal_name
Life Scijournal_title
Life sciencesauthors
Fang Y,Sun Y,Luo C,Gu J,Shi Z,Lu G,Silvestre JS,Chen Zdoi
10.1016/j.lfs.2020.117732subject
Has Abstractpub_date
2020-07-15 00:00:00pages
117732eissn
0024-3205issn
1879-0631pii
S0024-3205(20)30480-Xjournal_volume
253pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:Toll-like receptor (TLR)9 ligand CpG-oligodeoxynucleotide (CpG-ODN) exerts preconditioning in myocardial ischemia/reperfusion. We hypothesized a postconditioning effect of CpG-ODN in a murine closed-chest model of myocardial infarction. MATERIALS AND METHODS:C57BL/6 (12 weeks, male, WT) mice were instrumented at ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.09.029
更新日期:2014-12-05 00:00:00
abstract::The validity of a photoelectric method for continuous cerebral blood volume (CBV) measurement was tested and modified for the rat's brain. A new way of introducing a miniature light source between the two hemispheres and fixing a light sensitive silicone blue cell to the outer surface of the parietal bone was develope...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90163-3
更新日期:1986-11-03 00:00:00
abstract::Increased ventricular ANF-mRNA is observed as a compensating mechanism in response to many forms of overload including chronic exercise. It is unknown if exercise-induced increases in ANF expression are mediated at the transcriptional level and whether specific cis-element(s) may be involved. To study this, rats were ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01518-7
更新日期:2002-04-26 00:00:00
abstract:AIMS:Sepsis is a major cause of morbidity and mortality in the elderly population. In prior studies, we have shown that in vivo, the inflammatory response in aged animals is exaggerated as compared to young animals and that this response likely accounts for the increased morbidity and mortality. Part of this uncontroll...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.03.009
更新日期:2010-05-08 00:00:00
abstract::Memory impairment induced by intracerebroventricular (ICV) injection of streptozotocin (STZ) in rats is associated with impaired brain glucose and energy metabolism, oxidative stress and impaired cholinergic neurotransmission. Treatment with antioxidants and cholinergic agonists has been reported to produce beneficial...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.07.018
更新日期:2006-11-02 00:00:00
abstract::Angiotensin (AII) and atrial natriuretic peptide (ANP) exert opposite effects on phosphorylation of a 17.6 kDa nuclear protein from bovine adrenal glomerulosa cells. The protein was separated by sodium dodecyl sulfate electrophoresis and blotted onto polyvinylidene difluoride, and the N-terminal sequence was obtained....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90465-4
更新日期:1990-01-01 00:00:00
abstract::The plasma concentration of the dopamine (DA) metabolite, homovanillic acid (HVA), is used as an indicator of central nervous system dopaminergic activity. Using percutaneously inserted catheters we were able to obtain blood samples simultaneously from the right and left internal jugular veins. Veno-arterial HVA plasm...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90593-z
更新日期:1991-01-01 00:00:00
abstract::Methodological shortcomings present in elicitation of male sexual reflexes in anesthetized animals. The present study has demonstrated, however, that intraperitoneal (i.p.) injection of p-chloroamphetamine (PCA), an indirect serotonin (5-HT) agonist, elicited simultaneously both penile erection and ejaculation in anes...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00895-x
更新日期:2000-11-10 00:00:00
abstract:AIMS:This study investigates the effect of a new combination of glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane, Harpagophytum procumbens root extract (standardized to 3% harpagoside) and bromelain extract (GCMHB) on formalin-induced damage to cartilage tissue in the rat knee joint and evaluates t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.03.012
更新日期:2015-06-01 00:00:00
abstract::Sexual interactions have multiple effects on oxytocin systems in the brain. In the present study we observed that allowing estrogen-progesterone treated ovariectomized rats to be mounted ten times by a male significantly decreased the density (beta max) of thymic oxytocin receptors. Animals were ovariectomized and aft...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90467-h
更新日期:1993-01-01 00:00:00
abstract:AIMS:The rational basis that explains the benefits of exercise therapy on Chagas cardiomyopathy (ChC) is poorly understood. This study investigated the impact of an exercise program on exercise performance, heart parasitism, immunoinflammatory response, fibrogenesis, oxidative damage, and cardiomyocytes contractility i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.03.053
更新日期:2016-05-01 00:00:00
abstract:AIMS:Sarcopenia is characterized by muscle mass and strength loss and reduced physical activity. Branched-chain amino acids (BCAAs) were recently described as an activator of protein synthesis via mammalian target of rapamycin (mTOR) signaling for muscle atrophy. In cardiovascular diseases, excessive activation of the ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117593
更新日期:2020-06-01 00:00:00
abstract::To study the effects of volume overload and renal failure on plasma levels of immunoreactive atrial natriuretic hormone (IR-ANH), we measured levels of this hormone in normal subjects, in patients with advanced chronic renal failure (CRF) with and without clinically evident volume overload, and in patients with end-st...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90732-6
更新日期:1987-09-28 00:00:00
abstract::Studies were performed in conscious, chronically catheterized male Sprague-Dawley rats to investigate the effect of administered atrial natriuretic peptide (ANP) on blood pressure, renal hemodynamics and urinary electrolyte excretion. Studies were performed on young adult (3-4 month old) rats and on aging rats (18-24 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90066-z
更新日期:1990-01-01 00:00:00
abstract::To determine how protein kinase C (PKC) activity influences properties of the tetrodotoxin-resistant sodium current (TTX-R I(Na)) in neonatal rat nodose ganglion (NG) neurons, we assessed the effects of phorbol,-12-myristate,13-acetate (PMA), one of the PKC activators, and staurosporine, one of the PKC inhibitors, on ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.04.053
更新日期:2005-11-19 00:00:00
abstract::Human T-cell lymphotropic virus type 1 (HTLV-1) is associated with adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Only a limited percentage of infected individuals develop disease in response to the virus while the majority remain asymptomatic, and HAM/TSP...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2014.03.025
更新日期:2014-05-28 00:00:00
abstract::Uncoupling proteins (UCPs) are supposed to be involved in diet-induced thermogenesis. Their activities are usually elevated by feeding and reduced by fasting in normal animals. To investigate whether fasting affects the expression of UCPs mRNA in brown adipose tissue (BAT) of bilateral ventromedial hypothalamus (VMH)-...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00225-x
更新日期:2003-05-16 00:00:00
abstract::Studies were performed to compare in vitro and in vivo effects of morphine on the phagocytic function of murine peritoneal macrophages. Macrophage monolayers were incubated with Candida albicans for 30 min in the absence of autologous serum. Morphine added in vitro was found to decrease both the phagocytic activity (p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90122-j
更新日期:1993-01-01 00:00:00
abstract:AIMS:Uric acid nephropathy (UAN) is due to excessive uric acid, which leads to hyperuricemia and kidney damage via the deposition of urate microcrystals in the kidneys. Iridoid glycosides of Paederia scandens (IGPS) is a major active component isolated from the traditional Chinese herb P. scandens (LOUR.) MERRILL (Rubi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.08.013
更新日期:2012-10-05 00:00:00
abstract::Recent evidence suggests that the Type 1 opiate receptor (in rat striatal patches) is a mobile receptor which is able to adopt a mu, delta, or kappa opiate receptor ligand selectivity pattern under appropriate conditions. In this paper, we have investigated such a possibility for Type 2 opiate receptors which are visu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90183-7
更新日期:1982-10-18 00:00:00
abstract::Examination was made of the effects of 17 synthetic and naturally occurring flavonoids on human lymphocyte proliferation in the presence of concanavalin A as a mitogen. Twelve of the flavonoids examined were mono-hydroxy or methoxy derivatives. The mitogen-induced response of lymphocytes was evaluated from the extent ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90028-3
更新日期:1989-01-01 00:00:00
abstract::Stonustoxin (SNTX) is a 148 kDa, dimeric, hypotensive and lethal protein factor isolated from the venom of the stonefish Synanceja horrida. SNTX (10-320 ng/ml) progressively causes relaxation of endothelium-intact, phenylephrine (PE)-precontracted rat thoracic aortic rings. The SNTX-induced vasorelaxation was inhibite...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.01.058
更新日期:2007-04-10 00:00:00
abstract:AIMS:Acetylcholine (ACh) has been shown to increase ion and water excretion in the kidneys, resulting in hypotension. However, no evidence of renal parasympathetic innervation has been shown, and the source of ACh acting on nephrons is still unknown. The aim of the present study was to identify ACh-producing cells in t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.07.011
更新日期:2011-09-12 00:00:00
abstract::SR 142801 is the first potent and selective non-peptide antagonist of the tachykinin NK3 receptor. It inhibited [MePhe7]NKB binding to its receptor from various species, including humans. SR 142801 was a competitive antagonist of [MePhe7]NKB-mediated contractions of guinea-pig ileum and inhibited the acetylcholine rel...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00413-m
更新日期:1995-01-01 00:00:00
abstract::A variety of milk proteins including lactoferrin, angiogenin-1, alpha-lactalbumin, beta-lactoglobulin, lactoperoxidase, casein and the novel whey proteins lactogenin and glycolactin were tested for inhibitory activity toward human immunodeficiency virus-1 reverse transcriptase (HIV-1 RT), alpha-glucosidase, beta-gluco...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00853-5
更新日期:2000-10-20 00:00:00
abstract:AIMS:Although approving new anticonvulsants was a major breakthrough in the field of epilepsy control, so far we have met limited success in almost one third of patients suffering from epilepsy and a definite and reliable method is yet to be found. Levosimendan demonstrated neuroprotective effects and reduced mortality...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.11.006
更新日期:2017-01-01 00:00:00
abstract:AIMS:The purpose of this study was to determine a relatively short-term effect of feeding an α-linolenic acid (ALA, n-3)-restricted, linoleic acid (LA, n-6)-adequate diet on neurotrophin contents and protein kinase activities in brain regions of the mouse. MAIN METHODS:After feeding mice a safflower oil (SAF) diet (AL...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.09.003
更新日期:2010-10-09 00:00:00
abstract::The present study was undertaken to evaluate the cardioprotective potential of Curcuma longa (Turmeric) in the ischemia-reperfusion (I/R) model of myocardial infarction (MI). Wistar rats were divided into three groups and received saline orally (sham, control I/R group) and Curcuma longa 100 mg/kg (CL-100 treated grou...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.02.032
更新日期:2004-08-20 00:00:00
abstract::The present work aims to document, in a previously described animal model of Parkinson (double bilateral striatal injection of 6-OHDA), the possible modifications of circadian markers rhythms i.e. temperature (T), heart rate (H) and locomotor activity (A) registered continuously by telemetry, in order to evaluate a po...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00751-7
更新日期:2000-08-18 00:00:00
abstract::Immunotherapy with immune checkpoint inhibitors, such as anti-PD-1 drugs, is an area in increasing development for its efficacy and advantages in the treatment of advanced metastatic melanoma. In fact, immunotherapy has been the target of several and recent studies in different types of cancer, namely in melanoma, a g...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.117093
更新日期:2020-01-01 00:00:00