Chondroprotective effects of a new glucosamine combination in rats: Gene expression, biochemical and histopathological evaluation.

Abstract:

AIMS:This study investigates the effect of a new combination of glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane, Harpagophytum procumbens root extract (standardized to 3% harpagoside) and bromelain extract (GCMHB) on formalin-induced damage to cartilage tissue in the rat knee joint and evaluates this combination in comparison with another combination of glucosamine hydrochloride, chondroitin sulfate and methylsulfonylmethane (GKM). MATERIALS AND METHODS:Animals in the control group were injected with formalin into the knee joint (FCG). Animals in the GCMHB-500 group were given 500mg/kg GCMHB+formalin, and those in the GKM-500 group were given 500mg/kg GKM+formalin. Finally, a healthy group (HG) was also used. GCMHB and GKM were administered to rats orally once a day for 30days. At the end of this period, the rats were sacrificed and the levels of MDA, NO, 8-OH/Gua, and tGSH in the knee joint tissue were measured. Analysis of IL-1β and TNF-α gene expression was done and the tissue was evaluated histopathologically. KEY FINDINGS:MDA, NO and 8-OH/Gua levels and IL-1β and TNF-α gene expression were significantly lower in the GCMHB-500 group compared to the FCG group, whereas tGSH was significantly higher in the GCMHB-500 group than in the FCG group. No significant difference was found for the IL-1β, TNF-α and oxidant/antioxidant parameters between the GKM and FCG groups. The histopathological analysis showed that GCMHB could prevent damage to the cartilage joint, whereas GKM could not. SIGNIFICANCE:GCMHB may be used clinically by comparing with GKM in the treatment of osteoarthritis.

journal_name

Life Sci

journal_title

Life sciences

authors

Ucuncu Y,Celik N,Ozturk C,Turkoglu M,Cetin N,Kockara N,Sener E,Dundar C,Arslan A,Dogan H,Kurt N,Suleyman H

doi

10.1016/j.lfs.2015.03.012

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

31-7

eissn

0024-3205

issn

1879-0631

pii

S0024-3205(15)00168-X

journal_volume

130

pub_type

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