Abstract:
AIMS:This study investigates the effect of a new combination of glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane, Harpagophytum procumbens root extract (standardized to 3% harpagoside) and bromelain extract (GCMHB) on formalin-induced damage to cartilage tissue in the rat knee joint and evaluates this combination in comparison with another combination of glucosamine hydrochloride, chondroitin sulfate and methylsulfonylmethane (GKM). MATERIALS AND METHODS:Animals in the control group were injected with formalin into the knee joint (FCG). Animals in the GCMHB-500 group were given 500mg/kg GCMHB+formalin, and those in the GKM-500 group were given 500mg/kg GKM+formalin. Finally, a healthy group (HG) was also used. GCMHB and GKM were administered to rats orally once a day for 30days. At the end of this period, the rats were sacrificed and the levels of MDA, NO, 8-OH/Gua, and tGSH in the knee joint tissue were measured. Analysis of IL-1β and TNF-α gene expression was done and the tissue was evaluated histopathologically. KEY FINDINGS:MDA, NO and 8-OH/Gua levels and IL-1β and TNF-α gene expression were significantly lower in the GCMHB-500 group compared to the FCG group, whereas tGSH was significantly higher in the GCMHB-500 group than in the FCG group. No significant difference was found for the IL-1β, TNF-α and oxidant/antioxidant parameters between the GKM and FCG groups. The histopathological analysis showed that GCMHB could prevent damage to the cartilage joint, whereas GKM could not. SIGNIFICANCE:GCMHB may be used clinically by comparing with GKM in the treatment of osteoarthritis.
journal_name
Life Scijournal_title
Life sciencesauthors
Ucuncu Y,Celik N,Ozturk C,Turkoglu M,Cetin N,Kockara N,Sener E,Dundar C,Arslan A,Dogan H,Kurt N,Suleyman Hdoi
10.1016/j.lfs.2015.03.012subject
Has Abstractpub_date
2015-06-01 00:00:00pages
31-7eissn
0024-3205issn
1879-0631pii
S0024-3205(15)00168-Xjournal_volume
130pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Release of alpha-MSH from the pars intermedia melanotrope cells of Xenopus laevis is regulated by various classical neurotransmitters and neuropeptides. We have examined the effect of two of these regulatory substances, the neurotransmitter GABA and the CRF-related peptide sauvagine, on the adenylate cyclase system of...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90123-s
更新日期:1991-01-01 00:00:00
abstract::Oxidative stress induced cell injury is reported to contribute to the pathogenesis of cerebral ischemia. Reactive oxygen species such as hydrogen peroxide (H2O2) and superoxide radical along with nitric oxide and peroxynitrite generated during ischemia-reperfusion injury, causes the overactivation of poly (ADP-ribose)...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.07.034
更新日期:2006-11-10 00:00:00
abstract:AIMS:This study aims to evaluate the relationship between the amount of physical activity and different traditional and novel cardiometabolic risk factors, as well as atheroprotective agents, in male children and adolescents. MAIN METHODS:Cross-sectional study. A total of 337 male children and adolescents aged 7-14 ye...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.05.021
更新日期:2013-07-30 00:00:00
abstract::The isolated cat superior cervical ganglion (SCG) was labeled in vitro with either 3H-norepinephrine (3H-NE) or 3H-choline and stimulated through its preganglionic trunk. The release of 3H-NE and 3H-acetylcholine (3H-ACh) elicited by the stimulation was measured under control conditions and in the presence of drugs. T...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90371-6
更新日期:1985-04-29 00:00:00
abstract::Many hormones are known to induce the activity of ornithine decarboxylase (ODC), the first and rate-limiting enzyme for polyamine biosynthesis, in their target tissues. Using ovariectomized rats, we have compared the effects of sex hormones on ODC activity in the uterus and the kidney which contain estrogen and androg...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90219-h
更新日期:1990-01-01 00:00:00
abstract::The effect of maternal glucocorticoid depletion upon the fetal development of the organ of Zuckerkandl (OZ) in rats was determined. Maternal hypophysectomy at 13d8h gestation resulted in a fifty percent decrease in plasma corticosterone levels at 18d8h when compared to both sham operated and unoperated controls. No di...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90609-6
更新日期:1987-09-14 00:00:00
abstract::Evidence for the ability of the opiate antagonist naloxone to block a variety of metabolic effects exerted by morphine and non-opiate drugs is reviewed. Naloxone prevents or reverses the following effects in the rat: (a) the chronic morphine-induced increase in liver [NADPH]; (b) the consequent chronic morphine-induce...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90608-2
更新日期:1983-01-01 00:00:00
abstract::Even though conventional systemic doses of cimetidine and other histamine H(2) antagonists display minimal brain penetration, central nervous system (CNS) effects (including seizures and analgesia) have been reported after administration of these drugs in animals and man. To test the hypothesis that cimetidine-like dr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.05.020
更新日期:2004-10-08 00:00:00
abstract::Using an isolated, perfused rat lung model, we examined the hypoxic pulmonary vasoconstriction (HPV). We studied the alterations in HPV induced by the selective DA1 receptor agonist, fenoldopam, the selective DA1 antagonist, SCH 23390, as well as a combination of these agents. Fenoldopam significantly attenuated HPV. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90630-8
更新日期:1992-01-01 00:00:00
abstract::The in vivo dipyridamole treatment for 16 days produced a significant decrease in chick plasma cholesterol, mainly due to the esterified form. This effect was especially patent in the VLDL + LDL fraction. Similar results were observed in triglyceride content. To our knowledge, this is the first report on this hypolipi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90574-u
更新日期:1991-01-01 00:00:00
abstract::The effects of eight catechin derivatives on vascular tone in rat thoracic aorta were examined. Catechin derivatives (10 microM) potentiated the contractile response to phenylephrine in endothelium-intact arteries. The potentiations produced by EGCg and EGC were almost absent in endothelium-denuded arteries and abolis...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02080-5
更新日期:2002-10-11 00:00:00
abstract::The effects of isolation and water scheduling on mitogen induced lymphocyte proliferation were investigated. Isolated rats were animals which had been raised in group-housed conditions and then transferred to individual cages with ad lib access to water for a 1 or 2 week period. Water scheduled rats were maintained in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90472-9
更新日期:1988-01-01 00:00:00
abstract::Matrikines, peptides originating from the fragmentation of extracellular matrix proteins are identified to play important role in both health and disease. They possess biological activities, much different from their parent protein. Identification of such bioactive cryptic regions in the extracellular matrix proteins ...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.10.056
更新日期:2018-12-01 00:00:00
abstract::The temperature effects of naloxone and naltrexone (1-30 mg/kg) were examined in well habituated male rats. These drugs had a similar time course and potency, producing a dose-dependent hypothermia followed several hours later by a hyperthermia. A subsequent study found that not only did 30 mg/kg of naloxone or naltre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90563-7
更新日期:1987-03-16 00:00:00
abstract::Lesions of the serotonergic afferents to the hippocampus, by fimbrial transection or by 5,7-dihydroxytryptamine treatment, produce an increase in the Bmax of [3H]WB4101 to its nanomolar affinity binding site, with no effect on its picomolar affinity binding site or on [3H]prazosin binding. The nanomolar site is seroto...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90009-8
更新日期:1985-11-18 00:00:00
abstract::Ro41-0960 is a potent, fluorine containing COMT inhibitor which has be en reported to cross the blood brain barrier and to inhibit COMT in the brain. It is structurally similar to Ro40-7592 which is currently undergoing clinical trials in Parkinson's disease. Positron emission tomographic (PET) studies in baboon using...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02277-5
更新日期:1996-01-01 00:00:00
abstract::NMDA receptors are glutamate-regulated ion channels of critical importance for many neurophysiological and neuropathological processes. Mg2+ blocks the NMDA receptor by binding to the channel pore with an apparent affinity that depends on the membrane potential. We have investigated the effect of NMDA and the required...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00975-4
更新日期:2001-03-09 00:00:00
abstract::Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the biosynthesis of catecholamines. In a previous report we found that intracerebroventricular administration of nitric oxide (NO) generator sodium nitroprusside (SNP) to conscious male rats inhibited dose-dependently the TH activity of the median eminence (ME)....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00782-7
更新日期:2000-09-08 00:00:00
abstract::The binding of membrane immunoglobulin (mIg) by anti-Ig antibodies is known to initiate a mitogenic signal in B lymphocytes. Because in many instances growth control appears to be correlated with phosphokinase activity, as well as with alterations in cytoskeletal architecture, we asked the question whether antibodies ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90089-6
更新日期:1988-01-01 00:00:00
abstract::It has been shown that the main psychoactive component of marihuana, delta9-tetrahydrocannabinol (THC) has mainly inhibitory effects on the regulation of reproduction. Recently, the purification and availability of the endogenous ligand of the cannabinoid receptor, arachidonyl ethanolamide, anandamide, (ANA) and its s...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(99)00292-1
更新日期:1999-01-01 00:00:00
abstract::Leucogenenol a heterocyclic enolic thymothyroid hormone (MW 383) whose concentration in the serum regulates the rate at which already committed cells of the bone marrow develop into functional cells, was found to be associated in the thymus with a carrier protein. The carrier protein for leucogenenol is not precipitat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90245-5
更新日期:1984-07-30 00:00:00
abstract::After 7 years from its cloning, the transient receptor potential vanilloid type-1 (TRPV1) channel remains the sole membrane receptor mediating the pharmacological effects of the hot chilli pepper pungent component, capsaicin, and of the Euphorbia toxin, resiniferatoxin. Yet, this ion channel represents one of the most...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2005.05.021
更新日期:2005-08-19 00:00:00
abstract::The effects of leucine- and methionine-enkephalin, opiate peptides, on Ca2+ efflux from cultured bovine adrenal chromaffin cells were examined. These enkephalins stimulated the efflux of 45Ca2+ from cells in a concentration-dependent manner (10(-8) M-10(-6) M). Leucine-enkephalin did not increase the intracellular fre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00493-2
更新日期:1999-01-01 00:00:00
abstract::Leptin regulates glucose, lipid and energy homeostasis as well as feeding behavior, serving as a bridge between peripheral metabolically active tissues and the central nervous system (CNS). Indeed, this adipocyte-derived hormone, whose circulating levels mirror fat mass, not only exerts its anti-obesity effects mainly...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2015.05.012
更新日期:2015-11-01 00:00:00
abstract::In a partially depolarized guinea pig papillary muscle preparation, BAY K8644 stimulated voltage-operated calcium channels, promoting slow action potentials; this effect was dose-dependent over a concentration range of 3 X 10(-7) M to 3 X 10(-6) M. Isoproterenol and histamine also induced slow action potentials by sti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90651-x
更新日期:1986-07-28 00:00:00
abstract::The purpose of this study was to characterize behavioral interactions between glutamatergic and serotonergic 5-HT2 receptors. Both competitive (AP-5 [D-2-amino-5-phosphovalerate] and D-CPP [3-(2carboxypiperazine-4yl)-propylphosphonate]) and noncompetitive (MK-801 [dizocilpine], ketamine, dextrorphan and dextromethorph...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00519-0
更新日期:1998-01-01 00:00:00
abstract::Morphine and oxycodone are potent opioid analgesics most commonly used for the management of moderate to severe acute and chronic pain. Their clinical utility is limited by undesired side effects like analgesic tolerance, dependence, and withdrawal. We have previously demonstrated that endothelin-A (ETA) receptor anta...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2016.01.016
更新日期:2016-08-15 00:00:00
abstract::During the myocardial protection induced by ischemic preconditioning a reduction in myocardial metabolism occurs due to activation of the A1 adenosine receptors. This study investigates whether preconditioning changes both resting coronary flow and the magnitude of coronary reactive hyperemia and whether A1 adenosine ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00034-x
更新日期:1999-01-01 00:00:00
abstract::The aim of this study was to investigate the molecular mechanisms underlying the protective effects of hypoxia-inducible factor (HIF) signaling pathway activation in cardiomyocytes under anoxia-reoxygenation (A/R) injury. In this study, rat neonatal cardiomyocytes were pretreated with anti-Hif3A/Hif-3α siRNA or HIF-pr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.04.021
更新日期:2018-06-01 00:00:00
abstract::The effect of dihydropyridine calcium channel antagonist isradipine (PN 200-110) on morphine reinforcement has been investigated using i.v. self-administration test in rats. Rats were given the opportunity to self-administer a solution of morphine (1 mg/ml, i.v.) in a 1 hr limited access paradigm (FR = 1). Within 5-7 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00397-9
更新日期:1996-01-01 00:00:00