Restriction endonuclease characterization of resistant plasmids in Enterobacteriaceae isolated from children in the Sudan.

Abstract:

:The investigation of plasmid similarity is an important component in the surveillance of antimicrobial resistance and in the detection of epidemic plasmids. The use of restriction endonucleases in the classification of transferable, multiply-resistant plasmids from faecal Enterobacteriaceae isolated at the Children's Emergency Hospital, Khartoum was investigated. Twenty-four transconjugant plasmids, coding for 11 different resistance patterns, each of molecular weight 62 MDa, were studied using four restriction enzymes; Pst I, EcoR I, Hind III and Ava II. Fifteen different digest profiles were obtained. Restriction profiles discriminated between plasmids with differing resistance patterns and demonstrated homology of plasmids with common resistance patterns. Restriction endonuclease digest patterns provide a potentially rapid and reproducible method of plasmid classification, that could contribute towards surveillance systems in tropical countries with a high prevalence of antimicrobial resistance.

journal_name

Epidemiol Infect

authors

Shears P,Suliman G,Hart CA

doi

10.1017/s0950268800030892

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

487-96

issue

3

eissn

0950-2688

issn

1469-4409

journal_volume

103

pub_type

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