Abstract:
BACKGROUND AND PURPOSE:Ischemic injury triggers multiple pathological responses in the brain tissue, including spreading depolarizations across the cerebral cortex (cortical spreading depolarizations [CSD]). Microglia have been recently shown to play a significant role in the propagation of CSD. However, the intracellular responses of myeloid cells during ischemic stroke have not been investigated. METHODS:We have studied intracellular calcium activity in cortical microglia in the stroke model of the middle cerebral artery occlusion, using the murine Polr2a-based and Cre-dependent GCaMP5 and tdTomato reporter (PC::G5-tdT). High-speed 2-photon microscopy through cranial windows was employed to record signals from genetically encoded indicators of calcium. Inflammatory stimuli and pharmacological inhibition were used to modulate microglial calcium responses in the somatosensory cortex. RESULTS:In vivo imaging revealed periodical calcium activity in microglia during the hyperacute phase of ischemic stroke. This activity was more frequent during the first 6 hours after occlusion, but the amplitudes of calcium transients became larger at later time points. Consistent with CSD nature of these events, we reproducibly triggered comparable calcium transients with microinjections of potassium chloride (KCl) into adjacent cortical areas. Furthermore, lipopolysaccharide-induced peripheral inflammation, mimicking sterile inflammation during ischemic stroke, produced significantly greater microglial calcium transients during CSD. Finally, in vivo pharmacological analysis with CRAC (calcium release-activated channel) inhibitor CM-EX-137 demonstrated that CSD-associated microglial calcium transients after KCl microinjections are mediated at least in part by the CRAC mechanism. CONCLUSIONS:Our findings demonstrate that microglia participate in ischemic brain injury via previously undetected mechanisms, which may provide new avenues for therapeutic interventions.
journal_name
Strokejournal_title
Strokeauthors
Liu L,Kearns KN,Eli I,Sharifi KA,Soldozy S,Carlson EW,Scott KW,Sluzewski MF,Acton ST,Stauderman KA,Kalani MYS,Park M,Tvrdik Pdoi
10.1161/STROKEAHA.120.032766subject
Has Abstractpub_date
2021-01-01 00:00:00pages
274-283issue
1eissn
0039-2499issn
1524-4628journal_volume
52pub_type
杂志文章相关文献
STROKE文献大全abstract:BACKGROUND AND PURPOSE:The genetic basis of white matter hyperintensities (WMH) is still unknown. This study examines the heritability of WMH in both sexes and in different brain regions, and the influence of age. METHODS:Participants from the Older Australian Twins Study were recruited (n=320; 92 monozygotic and 68 d...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/STROKEAHA.116.012532
更新日期:2016-06-01 00:00:00
abstract::Cerebral cortical blood flow was measured in rabbits with the hydrogen clearance technique. The reactivity to CO2, tested by changing the end tidal CO2 (ETCO2) in steps from 2 to 6 volumes %, was highly dependent on the kind of anesthesia, being greatest under halothane and least under nitrous oxide. Reactivity to CO2...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.9.2.160
更新日期:1978-03-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Acute ischemic stroke in humans is associated with profound alterations in the immune system. Hallmarks of this stroke-induced immunodepression syndrome are: lymphocytopenia, impairment of T helper cell and monocyte function. We studied which stroke-specific factors predict these immunologic alte...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/STROKEAHA.109.557967
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Proven behavioral assessment strategies for testing potential therapeutic agents in rat stroke models are needed. Few studies include tasks that demand higher levels of sensorimotor and cognitive function. Because behavioral outcome and rate of recovery vary among ischemia models, there is a need...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/hs1101.097397
更新日期:2001-11-01 00:00:00
abstract:BACKGROUND AND PURPOSE:To test the safety and recanalization efficacy of intra-arterial local delivery of plasminogen activators in acute ischemic stroke, a randomized trial of recombinant pro-urokinase (rpro-UK) versus placebo was undertaken in patients with angiographically documented proximal middle cerebral artery ...
journal_title:Stroke
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1161/01.str.29.1.4
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND AND PURPOSE:This study was designed to investigate the correlation between reflex sympathetic dystrophy syndrome (RSDS) in hemiplegic patients and spontaneous electromyographic (EMG) activity, as well as to determine the predictive value of spontaneous EMG activity in early diagnosis of RSDS. METHODS:An EMG...
journal_title:Stroke
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Spontaneous dissection of the cervical internal carotid artery (sICAD) is mainly assessed with MRI and magnetic resonance angiography (MRA), which are not always at hand. In contrast, color duplex sonography (CDS) is readily available. We undertook this prospective study to examine the accuracy o...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.STR.0000198811.65068.16
更新日期:2006-02-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Of few prospective studies that have focused on the relationship between fibrinogen and ischemic stroke (IS) in Asian populations, the findings were inconsistent with those conducted in Western countries. Therefore, we aimed to investigate the temporal relationship between fibrinogen levels (plus...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/STROKEAHA.108.540492
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Progression of asymptomatic carotid artery stenosis (ACAS) in patients with >50% luminal narrowing is considered a potential risk factor for ischemic stroke; however, subclinical molecular biomarkers of ACAS progression are lacking. Recent studies suggest a regulatory function for several microRN...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/STROKEAHA.116.013650
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND AND PURPOSE:The relationship between arterial recanalization, infarct size, and outcome in patients treated with intravenous thrombolytics remains unclear. Therefore, we aimed to determine the time course of recombinant tissue plasminogen activator (rtPA)-induced recanalization in patients with cardioembolic...
journal_title:Stroke
pub_type: 临床试验,杂志文章
doi:10.1161/hs1201.99821
更新日期:2001-12-01 00:00:00
abstract::Background and Purpose- Acetylsalicylic acid and clopidogrel are the 2 main antithrombotic drugs for secondary prevention in patients with ischemic stroke (IS) without indication for anticoagulation. Because of their limited efficacy and potential side effects, novel antiplatelet agents are urgently needed. Cilostazol...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/STROKEAHA.118.023664
更新日期:2019-02-01 00:00:00
abstract:BACKGROUND AND PURPOSE:The greater mortality and residual physical impairments among black stroke patients may be attributable to differential utilization of rehabilitation services. This report examines, within an equal-access healthcare system, racial differences in time to initiation of stroke rehabilitation service...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.STR.0000060028.60365.5D
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Transcranial Doppler (TCD) and carotid duplex (CD) provide rapid and safe screening for stroke patients but are highly operator dependent. We explored the feasibility of telemedicine (TM)-guided TCD/CD administered by a health care provider inexperienced with ultrasound. METHODS:Dual video scree...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.STR.0000196988.45318.97
更新日期:2006-01-01 00:00:00
abstract::Cystatin C, a protein inhibitor of lysosomal cysteine proteinases, was demonstrated by immunohistochemical techniques to be present in the birefringent amyloid deposits of the small arteries in the cerebrum, cerebellum, and leptomeninges of 10 Icelandic individuals with hereditary cerebral hemorrhage with amyloidosis....
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.18.2.431
更新日期:1987-03-01 00:00:00
abstract::Perforating branches of the middle cerebral arteries (MCA) were examined on the forebrain hemispheres of fourteen human brains. It was noticed that their intracerebral segments arose from the MCA main trunk, and its terminal and collateral (cortical) branches. They terminated in certain parts of the basal ganglia and ...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.16.6.1022
更新日期:1985-11-01 00:00:00
abstract:BACKGROUND AND PURPOSE:A precise definition of genetic factors responsible for common forms of stroke is still lacking. The purpose of the present study was to investigate the contributory role of the genes encoding atrial natriuretic peptide (ANP) and type A natriuretic peptide receptor (NPRA) in humans' susceptibilit...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.STR.0000119381.52589.AB
更新日期:2004-04-01 00:00:00
abstract:BACKGROUND AND PURPOSE:We tested the hypothesis that sildenafil, a phosphodiesterase type 5 (PDE5) inhibitor, promotes functional recovery and neurogenesis after stroke. METHODS:Male Wistar rats were subjected to embolic middle cerebral artery occlusion. Sildenafil (Viagra) was administered orally for 7 consecutive da...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.0000034399.95249.59
更新日期:2002-11-01 00:00:00
abstract::Occlusion of the anterior cerebral artery is a rare condition. A review of CT scans from 413 patients with ischemic infarction confirmed this low relative incidence: only 3% of CT scans evidencing infarction involved the territory of the anterior cerebral artery. Four major mechanisms of anterior cerebral artery occlu...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.14.6.952
更新日期:1983-11-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Autosomal dominant hypertension with brachydactyly features severe hypertension that causes stroke usually before the age of 50 years. We recently characterized the hypertension as featuring normal renin, aldosterone, and catecholamine responses and mapped the gene responsible to chromosome 12p. ...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.28.9.1749
更新日期:1997-09-01 00:00:00
abstract::The morphologic description and measurements of endarterectomy specimens are usually believed to be accurate and are used as the gold standard against which the findings of diagnostic procedures are judged. Pathology data on 289 endarterectomy specimens from five participating centers and the corresponding angiography...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.19.3.289
更新日期:1988-03-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Homocysteine has been linked to increased risk of ischemic stroke and other cardiovascular events. Matrix degradation and inflammation play an important role in these disorders, and we have demonstrated increased levels of matrix-degrading enzymes and inflammatory cytokines in hyperhomocysteinemi...
journal_title:Stroke
pub_type: 杂志文章,随机对照试验
doi:10.1161/STROKEAHA.108.522995
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Randomized clinical trials testing aspirin in relatively low-risk, middle-aged people have consistently shown small increases in stroke associated with aspirin use. We analyzed the relationship between the regular use of aspirin and incident ischemic and hemorrhagic stroke among people aged 65 ye...
journal_title:Stroke
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1161/01.str.29.5.887
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Stroke leads to a reduction in bone mineral density, altered calcium homeostasis, and an increase in hip fractures. Vitamin D deficiency is well documented in long-term stroke survivors and is associated with post-stroke hip fractures. Less is known regarding levels in acute stroke. METHODS:We c...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.STR.0000195184.24297.c1
更新日期:2006-01-01 00:00:00
abstract::Perioperative cerebral infarction occurs in less than 1% of general surgical procedures; the mechanism is usually unknown. The clinical features of 12 consecutive perioperative strokes were retrospectively reviewed. Although intraoperative hypotension was frequent, onset of deficit occurred postoperatively in 83% and ...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.13.6.766
更新日期:1982-11-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Desaturation during swallowing may help to identify aspiration in stroke patients. This study investigated pulse oximetry, bedside swallowing assessment (BSA), and videofluoroscopy as tests for detecting aspiration after stroke. METHODS:Swallowing was assessed in 189 stroke patients (mean+/-SD a...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.STR.0000248758.32627.3b
更新日期:2006-12-01 00:00:00
abstract::To elucidate the role of the internal elastic lamina in the development of cerebral aneurysm, the bifurcation of the anterior cerebral artery and olfactory artery was histologically studied in control and experimental rats treated with unilateral carotid ligation and renal hypertension. Various stages of aneurysm form...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.19.4.507
更新日期:1988-04-01 00:00:00
abstract:BACKGROUND AND PURPOSE:The etiology of stroke in the young is different from that in older patients and remains unknown in almost one third of the cases. To gain further insight into both pathogenic and etiologic determinants, we prospectively studied a large number of consecutive young adults with focal cerebral ische...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/01.str.24.3.362
更新日期:1993-03-01 00:00:00
abstract:BACKGROUND AND PURPOSE:The overall burden of prior infections may contribute to atherosclerosis and stroke risk. We hypothesized that serological evidence of common infections would be associated with carotid plaque thickness in a multiethnic cohort. METHODS:Antibody titers to 5 common infectious microorganisms (ie, C...
journal_title:Stroke
pub_type: 杂志文章
doi:10.1161/STROKEAHA.109.571299
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Outcome after mechanical thrombectomy for ischemic stroke may be influenced by blood pressure (BP). This study aims to assess the association of BP changes during general anesthesia versus conscious sedation with functional outcome after mechanical thrombectomy. METHODS:SIESTA (Sedation vs Intub...
journal_title:Stroke
pub_type: 杂志文章,随机对照试验
doi:10.1161/STROKEAHA.117.019709
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Patients with carotid occlusive disease and stage 2 cerebral hemodynamic failure, characterized by an increased oxygen extraction fraction (OEF) as measured by positron emission tomography (PET) and otherwise known as misery perfusion, have a high risk of cerebral ischemia and subsequent stroke. ...
journal_title:Stroke
pub_type: 临床试验,杂志文章
doi:10.1161/01.str.0000027638.19392.7e
更新日期:2002-09-01 00:00:00