Fast infrared spectroscopy of protein dynamics: advancing sensitivity and selectivity.

abstract：:In all contemporary organisms, the active site of the ribosome--the peptidyl transferase center--catalyzes two distinct reactions, peptide bond formation between peptidyl-tRNA and aminoacyl-tRNA as well as the hydrolysis of peptidyl-tRNA with the help of a release factor. However, when provided with appropriate substr...

journal_title：Current opinion in structural biology

authors： Rodnina MV

更新日期：2013-08-01 00:00:00

abstract：:As a result of the recent enormous technological progress, experimental structure determination has become an integral part of the development of drugs against disease-related target proteins. The post-translational modification of proteins is an important regulatory process in living organisms; one such example is ly...

journal_title：Current opinion in structural biology

authors： Mittl PR,Grütter MG

更新日期：2006-12-01 00:00:00

abstract：:Understanding the structural rules that govern specific, high-affinity binding characteristic of aptamer-protein interactions is important in view of the increasing use of aptamers across many applications. From the modest number of 16 aptamer-protein structures currently available, trends are emerging. The flexible p...

journal_title：Current opinion in structural biology

authors： Gelinas AD,Davies DR,Janjic N

更新日期：2016-02-01 00:00:00

abstract：:In the prokaryotic CRISPR-Cas adaptive immune systems, a CRISPR RNA (crRNA) assembles with multiple or single Cas proteins to form crRNA ribonucleoprotein (crRNP) effector complexes, responsible for the destruction of invading genetic elements. Although the mechanisms of target recognition and cleavage by the crRNP ef...

journal_title：Current opinion in structural biology

authors： Nishimasu H,Nureki O

更新日期：2017-04-01 00:00:00

abstract：:Heparan sulfate (HS) is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface. ...

journal_title：Current opinion in structural biology

authors： Mohammadi M,Olsen SK,Goetz R

更新日期：2005-10-01 00:00:00

abstract：:The structure of the visual pigment rhodopsin in the dark state was first investigated by electron microscopy (EM). More recently, rhodopsin has been crystallised in two different space groups--a tetragonal P4(1) crystal form and a trigonal P3(1) packing arrangement. The structures of the pigment, determined by X-ray ...

journal_title：Current opinion in structural biology

authors： Schertler GF

更新日期：2005-08-01 00:00:00

abstract：:Progress in the synthesis, purification and crystallization of RNA has resulted in the determination of several X-ray crystal structures of RNA molecules over the past few years. Methods proven and under development will lead to future structure determinations and shed light on the structural basis for RNA's many func...

journal_title：Current opinion in structural biology

authors： Lietzke SE,Barnes CL,Kundrot CE

更新日期：1995-10-01 00:00:00

abstract：:Can a stereochemical recognition code explain sequence-specific protein-nucleic acid interactions? Whereas a code that is generally applicable to DNA-binding proteins of all known structural families is unattainable, the indications are that a code can describe at least some of the interactions of classical zinc finge...

journal_title：Current opinion in structural biology

authors： Choo Y,Klug A

更新日期：1997-02-01 00:00:00

abstract：:The 26S proteasome is the essential compartmental protease in eukaryotic cells required for the ubiquitin-dependent clearance of damaged polypeptides and obsolete regulatory proteins. Recently, a combination of high-resolution structural, biochemical, and biophysical studies has provided crucial new insights into the ...

journal_title：Current opinion in structural biology

authors： Greene ER,Dong KC,Martin A

更新日期：2020-04-01 00:00:00

abstract：:Lipidic mesophases have been used to produce diffraction-quality crystals of several membrane proteins. The mechanism by which the method works is a mystery. The thrust is to continue to use it whilst deciphering the underlying mechanism and solving the second 'phase problem' in membrane protein crystallography. The m...

journal_title：Current opinion in structural biology

authors： Caffrey M

更新日期：2000-08-01 00:00:00

abstract：:Condensation of DNA in the nucleosome takes advantage of its double-helical architecture. The DNA deforms at sites where the base pairs face the histone octamer. The largest so-called kink-and-slide deformations occur in the vicinity of arginines that penetrate the minor groove. Nucleosome structures formed from the 6...

journal_title：Current opinion in structural biology

authors： Olson WK,Zhurkin VB

更新日期：2011-06-01 00:00:00

abstract：:Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combinati...

journal_title：Current opinion in structural biology

authors： Mackereth CD,Sattler M

更新日期：2012-06-01 00:00:00

abstract：:Computational methods play an important role at all stages of the process of determining protein-protein interactions. They are used to predict potential interactions, to validate the results of high-throughput interaction screens and to analyze the protein networks inferred from interaction databases. ...

journal_title：Current opinion in structural biology

authors： Salwinski L,Eisenberg D

更新日期：2003-06-01 00:00:00

abstract：:Integrative structural modeling uses multiple types of input information and proceeds in four stages: (i) gathering information, (ii) designing model representation and converting information into a scoring function, (iii) sampling good-scoring models, and (iv) analyzing models and information. In the first stage, unc...

journal_title：Current opinion in structural biology

authors： Schneidman-Duhovny D,Pellarin R,Sali A

更新日期：2014-10-01 00:00:00

abstract：:The past decade has seen a revolution in our ability to engineer designer enzymes using genetic tools that mimic evolution on a laboratory timescale. Many excellent examples of directed evolution applied to a wide range of enzymes have clearly demonstrated its future role in adapting enzymes for use in the chemical in...

journal_title：Current opinion in structural biology

authors： Dalby PA

更新日期：2003-08-01 00:00:00

abstract：:The past years have seen tremendous progress towards understanding how arrestins recognize phosphorylated G protein-coupled receptors (GPCRs). Two arrestin crystal structures, one of a pre-activated splice variant and one bound to a GPCR phosphopeptide, provided insights into the conformational changes upon phosphate ...

journal_title：Current opinion in structural biology

authors： Ostermaier MK,Schertler GF,Standfuss J

更新日期：2014-12-01 00:00:00

abstract：:Due to the contribution of drug-target binding kinetics to drug efficacy, there is a high level of interest in developing methods to predict drug-target binding kinetic parameters. During the review period, a wide range of enhanced sampling molecular dynamics simulation-based methods has been developed for computing d...

journal_title：Current opinion in structural biology

authors： Nunes-Alves A,Kokh DB,Wade RC

更新日期：2020-10-01 00:00:00

abstract：:The three-dimensional structures of several biotin-binding proteins are now known, giving insights into the molecular architecture of the binding sites for biotin. In combination with biochemical and computational approaches, these structural insights provide the basis for our present understanding of biotin-protein i...

journal_title：Current opinion in structural biology

authors： Lindqvist Y,Schneider G

更新日期：1996-12-01 00:00:00

abstract：:Over recent years, nuclear magnetic resonance (NMR) spectroscopy has developed into a powerful mechanistic tool for the investigation of G protein-coupled receptors (GPCRs). NMR provides insights which underpin the dynamic nature of these important receptors and reveals experimental evidence for a complex conformation...

journal_title：Current opinion in structural biology

authors： Bostock MJ,Solt AS,Nietlispach D

更新日期：2019-08-01 00:00:00

abstract：:Neutron diffraction provides different information from X-ray diffraction, because neutrons are scattered by atomic nuclei, whereas X-rays are scattered by electrons. One of the key advantages of neutron crystallography is the ability to visualize hydrogen and deuterium atoms, making it possible to observe the protona...

journal_title：Current opinion in structural biology

authors： Nakamura A,Ishida T,Samejima M,Igarashi K

更新日期：2016-10-01 00:00:00

abstract：:The RNA exosome participates in the degradation and processing of a wide range of RNA molecules. Recent advances in understanding how the exosome is organized and functions largely stem from structural studies. Crystal structures of archaeal exosomes bound to RNA and of the corresponding nine-subunit human exosome cor...

journal_title：Current opinion in structural biology

authors： Lorentzen E,Basquin J,Conti E

更新日期：2008-12-01 00:00:00

abstract：:Newly synthesized proteins leave the ribosome through a narrow tunnel in the large subunit. During ongoing synthesis, nascent protein chains are particularly sensitive to aggregation and degradation because they emerge from the ribosome in an unfolded state. In bacteria, the first protein to interact with nascent chai...

journal_title：Current opinion in structural biology

authors： Maier T,Ferbitz L,Deuerling E,Ban N

更新日期：2005-04-01 00:00:00

abstract：:The ribosome undergoes numerous large-scale conformational changes during protein synthesis, but the molecular basis for these changes have been unclear. Recent cryo-electron microscopic and X-ray crystallographic structures of both the bacterial and eukaryotic ribosome now provide snapshots of the wide range of motio...

journal_title：Current opinion in structural biology

authors： Noeske J,Cate JH

更新日期：2012-12-01 00:00:00

abstract：:Multivalent molecules with a precise array of recognition elements that interact with specific cell types are important for characterizing the topology of molecules on a cell surface. Applications ranging from the control of cellular signaling to drug delivery and tissue imaging rely on these surface-mapping molecules...

journal_title：Current opinion in structural biology

authors： Lee Y,Sampson NS

更新日期：2006-08-01 00:00:00

abstract：:Covalent modifications of histone proteins regulate a wide variety of cellular processes. Methylation of histone H3K79 and H3K4 is associated with active transcription and is catalyzed by Dot1L and Set1, respectively. Both Dot1L and Set1 are activated by prior ubiquitination of histone H2B on K120 in a process termed ...

journal_title：Current opinion in structural biology

authors： Worden EJ,Wolberger C

更新日期：2019-12-01 00:00:00

abstract：:Changing macromolecular conformations and complexes are critical features of cellular networks, typified by DNA damage response pathways that are essential to life. These fluctuations enhance the specificity of macromolecular recognition and catalysis, and enable an integrated functioning of pathway components, ensuri...

journal_title：Current opinion in structural biology

authors： Perry JJ,Cotner-Gohara E,Ellenberger T,Tainer JA

更新日期：2010-06-01 00:00:00

abstract：:Polyketide synthases (PKSs) form a large family of multifunctional proteins involved in the biosynthesis of diverse classes of natural products. Architecturally at least three different types of PKSs have been discovered in the microbial world and recent years have revealed tremendous versatility of PKSs, both in term...

journal_title：Current opinion in structural biology

authors： Gokhale RS,Sankaranarayanan R,Mohanty D

更新日期：2007-12-01 00:00:00

abstract：:There has been fantastic progress in solving GPCR crystal structures. However, the ability of X-ray crystallography to guide the drug discovery process for GPCR targets is limited by the availability of accurate tools to explore receptor-ligand interactions. Visual inspection and molecular mechanics approaches cannot ...

journal_title：Current opinion in structural biology

authors： Heifetz A,James T,Southey M,Morao I,Aldeghi M,Sarrat L,Fedorov DG,Bodkin MJ,Townsend-Nicholson A

更新日期：2019-04-01 00:00:00

abstract：:Assembly line polyketide synthases (PKSs) are remarkable biosynthetic machines with considerable potential for structure-based engineering. Several types of protein-protein interactions, both within and between PKS modules, play important roles in the catalytic cycle of a multimodular PKS. Additionally, vectorial bios...

journal_title：Current opinion in structural biology

authors： Robbins T,Liu YC,Cane DE,Khosla C

更新日期：2016-12-01 00:00:00

abstract：:The wide occurrence of sialic acids (Sia) in various chemical forms linked as monomers or polymers in an outstanding position in a multitude of complex carbohydrates of animals and microorganisms renders them as most versatile function modulators in cell biology and pathology. A survey is presented of recent advances ...

journal_title：Current opinion in structural biology

authors： Schauer R

更新日期：2009-10-01 00:00:00