Abstract:
:Assembly line polyketide synthases (PKSs) are remarkable biosynthetic machines with considerable potential for structure-based engineering. Several types of protein-protein interactions, both within and between PKS modules, play important roles in the catalytic cycle of a multimodular PKS. Additionally, vectorial biosynthesis is enabled by the energetic coupling of polyketide chain elongation to the channeling of intermediates between successive modules. A combination of high-resolution analysis of smaller PKS components and lower resolution characterization of intact modules and bimodules has yielded insights into the structure and organization of a prototypical assembly line PKS. This review discusses our understanding of key structure-function relationships in this family of megasynthases, along with a recap of key unanswered questions in the field.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Robbins T,Liu YC,Cane DE,Khosla Cdoi
10.1016/j.sbi.2016.05.009subject
Has Abstractpub_date
2016-12-01 00:00:00pages
10-18eissn
0959-440Xissn
1879-033Xpii
S0959-440X(16)30044-6journal_volume
41pub_type
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