Long-timescale molecular dynamics simulations of protein structure and function.

abstract：:Crystallographic studies of a number of aminoacyl-tRNA synthetases and their complexes with ATP, amino acid and cognate tRNA are leading to an increasingly detailed picture of how these sophisticated enzymes function. Within the two distinct structural classes of ten synthetases, many common features are apparent, alt...

journal_title：Current opinion in structural biology

authors： Cusack S

更新日期：1997-12-01 00:00:00

abstract：:Protein biogenesis integrates multiple finely regulated mechanisms, ensuring nascent polypeptide chains are correctly enzymatically processed, targeted to membranes and folded to native structure. Recent studies show that the cellular translation machinery serves as hub that coordinates the maturation events in space ...

journal_title：Current opinion in structural biology

authors： Gloge F,Becker AH,Kramer G,Bukau B

更新日期：2014-02-01 00:00:00

abstract：:C-type lectins are the largest and most diverse family of mammalian carbohydrate-binding proteins. They share a common protein fold, which provides the unifying basis for calcium-mediated carbohydrate recognition. Their involvement in a multitude of biological functions is remarkable. Here, we review the variety of ta...

journal_title：Current opinion in structural biology

authors： Keller BG,Rademacher C

更新日期：2020-06-01 00:00:00

abstract：:Maintenance of telomeres by the enzyme telomerase is essential for genomic stability and cell viability in ciliates, vertebrates and yeast. The minimal components of telomerase required for catalytic activity are the telomerase reverse transcriptase (TERT) protein and the template-containing telomerase RNA (TER). Rece...

journal_title：Current opinion in structural biology

authors： Theimer CA,Feigon J

更新日期：2006-06-01 00:00:00

abstract：:The observed distribution of protein structures can give us important clues about the underlying evolutionary process, imposing important constraints on possible models. The availability of results from an increasing number of genome projects has made the development of these models an active area of research. Models ...

journal_title：Current opinion in structural biology

authors： Goldstein RA

更新日期：2008-04-01 00:00:00

abstract：:In all contemporary organisms, the active site of the ribosome--the peptidyl transferase center--catalyzes two distinct reactions, peptide bond formation between peptidyl-tRNA and aminoacyl-tRNA as well as the hydrolysis of peptidyl-tRNA with the help of a release factor. However, when provided with appropriate substr...

journal_title：Current opinion in structural biology

authors： Rodnina MV

更新日期：2013-08-01 00:00:00

abstract：:Heparan sulfate (HS) is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface. ...

journal_title：Current opinion in structural biology

authors： Mohammadi M,Olsen SK,Goetz R

更新日期：2005-10-01 00:00:00

abstract：:The past decade has seen a revolution in our ability to engineer designer enzymes using genetic tools that mimic evolution on a laboratory timescale. Many excellent examples of directed evolution applied to a wide range of enzymes have clearly demonstrated its future role in adapting enzymes for use in the chemical in...

journal_title：Current opinion in structural biology

authors： Dalby PA

更新日期：2003-08-01 00:00:00

abstract：:Conformational changes are an essential feature of most molecular processes in biology. Optical tweezers have emerged as a powerful tool for probing conformational dynamics at the single-molecule level because of their high resolution and sensitivity, opening new windows on phenomena ranging from folding and ligand bi...

journal_title：Current opinion in structural biology

authors： Ritchie DB,Woodside MT

更新日期：2015-10-01 00:00:00

abstract：:Recent developments in implicit solvent models may be compared in terms of accuracy and computational efficiency. Based on improvements in the accuracy of generalized Born methods and the speed of Poisson-Boltzmann solvers, it appears that the two techniques are converging to a point at which both will be suitable for...

journal_title：Current opinion in structural biology

authors： Baker NA

更新日期：2005-04-01 00:00:00

abstract：:Aromatic polyketides are secondary metabolites that afford some of the most common antibiotics and anti-cancer drugs currently in clinical use. Not least because of their medical importance, the biosynthesis of these compounds has attracted considerable interest during the past few years; important advances have been ...

journal_title：Current opinion in structural biology

authors： Schneider G

更新日期：2005-12-01 00:00:00

abstract：:Hydrogen-deuterium exchange (HDX) mass spectrometry (MS) can provide information about proteins that can be challenging to obtain by other means. Structure/function relationships, binding interactions, and the effects of modification have all been measured with HDX MS for a diverse and growing array of signaling prote...

journal_title：Current opinion in structural biology

authors： Harrison RA,Engen JR

更新日期：2016-12-01 00:00:00

abstract：:Achieving the goals of structural genomics initiatives depends on the outcomes of two groups of factors: the number and distribution of experimentally determined protein structures, and our ability to assign novel proteins to known structures (fold recognition) and use them to build models (modeling). The quality of t...

journal_title：Current opinion in structural biology

authors： Friedberg I,Jaroszewski L,Ye Y,Godzik A

更新日期：2004-06-01 00:00:00

abstract：:Cells are equipped with cosolvents that modulate protein folding and aggregation to withstand water stress. The effect of cosolvents on the aggregation rates depends on whether the polypeptide sequence is an intrinsically disordered protein (IDP) or can fold into a specific native structure. Cosolvents, which act as d...

journal_title：Current opinion in structural biology

authors： Reddy G,Muttathukattil AN,Mondal B

更新日期：2020-02-01 00:00:00

abstract：:Cohesin-Dockerin interactions are at the core of cellulosomal assembly and organization. They are highly specific and form stable complexes, allowing cellulosomes to adopt distinct conformations. Each cellulosomal system seems to have a particular organizational strategy that can vary in complexity according to the na...

journal_title：Current opinion in structural biology

authors： Bule P,Pires VM,Fontes CM,Alves VD

更新日期：2018-04-01 00:00:00

abstract：:Connecting three dimensional structure and affinity is analogous to seeking the 'Archimedean point', a vantage point from where any observer can quantitatively perceive the subject of inquiry. Here we review current knowledge and challenges that lie ahead of us in the quest for this Archimedean point. We argue that cu...

journal_title：Current opinion in structural biology

authors： Kastritis PL,Bonvin AM

更新日期：2013-12-01 00:00:00

abstract：:Ring-shaped nucleic acid translocases and helicases catalyze the directed and processive movement of nucleic acid strands to support essential transactions such as replication, transcription, and chromosome partitioning. Assembled typically as hexamers, ring helicase/translocase systems use coordinated cycles of nucle...

journal_title：Current opinion in structural biology

authors： O'Shea VL,Berger JM

更新日期：2014-04-01 00:00:00

abstract：:ATP-dependent initiation factors help process replication origins and coordinate replisome assembly to control the onset of DNA synthesis. Although the specific properties and regulatory mechanisms of initiator proteins can vary greatly between different organisms, certain nucleotide-binding elements and assembly patt...

journal_title：Current opinion in structural biology

authors： Duderstadt KE,Berger JM

更新日期：2013-02-01 00:00:00

abstract：:TolC and its many homologues comprise an alpha-helical transperiplasmic tunnel embedded in the bacterial outer membrane by a contiguous beta-barrel channel, providing a large exit duct for diverse substrates. The 'channel-tunnel' is closed at its periplasmic entrance, but can be opened by an 'iris-like' mechanism when...

journal_title：Current opinion in structural biology

authors： Koronakis V,Andersen C,Hughes C

更新日期：2001-08-01 00:00:00

abstract：:Understanding the structural rules that govern specific, high-affinity binding characteristic of aptamer-protein interactions is important in view of the increasing use of aptamers across many applications. From the modest number of 16 aptamer-protein structures currently available, trends are emerging. The flexible p...

journal_title：Current opinion in structural biology

authors： Gelinas AD,Davies DR,Janjic N

更新日期：2016-02-01 00:00:00

abstract：:The alpha helices of transmembrane proteins interact to form higher order structures. These interactions are frequently mediated by packing motifs (such as GxxxG) and polar residues. Recent structural data have revealed that small sidechains are able to both stabilize helical membrane proteins and allow conformational...

journal_title：Current opinion in structural biology

authors： Curran AR,Engelman DM

更新日期：2003-08-01 00:00:00

abstract：:The past twelve months have seen a renewal of interest in the therapeutic potential of members of the tumour necrosis factor receptor family and their cytokine ligands. This biomedical interest has spawned a number of structural studies, which have significantly deepened our understanding of the molecular basis for th...

journal_title：Current opinion in structural biology

authors： Jones EY

更新日期：2000-12-01 00:00:00

abstract：:The major component of bacterial cell wall is peptidoglycan (PG), a complex polymer formed by long glycan chains cross-linked by peptide stems. PG is in constant equilibrium requiring well-orchestrated coordination between synthesis and degradation. The resulting cell-wall fragments can be recycled, act as messengers ...

journal_title：Current opinion in structural biology

authors： Alcorlo M,Martínez-Caballero S,Molina R,Hermoso JA

更新日期：2017-06-01 00:00:00

abstract：:Among the crystal structures of lectins determined recently, three--snowdrop lectin, jacalin and amaranthin--represent new lectin families. Their polypeptide folds share remarkably similar features and consist exclusively of beta structure. Autonomously folded beta-sheet subdomains, inter-related by a pseudothreefold ...

journal_title：Current opinion in structural biology

authors： Wright CS

更新日期：1997-10-01 00:00:00

abstract：:The structure of the visual pigment rhodopsin in the dark state was first investigated by electron microscopy (EM). More recently, rhodopsin has been crystallised in two different space groups--a tetragonal P4(1) crystal form and a trigonal P3(1) packing arrangement. The structures of the pigment, determined by X-ray ...

journal_title：Current opinion in structural biology

authors： Schertler GF

更新日期：2005-08-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) participate in virtually all physiological processes. They constitute the largest and most structurally conserved family of signaling molecules. Several class C GPCRs have been shown to exist as dimers in their active form and growing evidence indicates that many, if not all, class ...

journal_title：Current opinion in structural biology

authors： Fotiadis D,Jastrzebska B,Philippsen A,Müller DJ,Palczewski K,Engel A

更新日期：2006-04-01 00:00:00

abstract：:Specific, noncovalent binding of biomolecules can only be understood by considering structural, thermodynamic, and kinetic issues. The theoretical foundations for such analyses have been clarified in the past year. Computational techniques for both particle-based and continuum models continue to improve and to yield u...

journal_title：Current opinion in structural biology

authors： McCammon JA

更新日期：1998-04-01 00:00:00

abstract：:The past year has seen some major advances in the area of carbohydrate synthesis using chemical methods. Progress in all areas of synthetic methodology, including new protecting groups and coupling methods, has been reported. A number of complex carbohydrate structures have been prepared using known, as well as new, m...

journal_title：Current opinion in structural biology

authors： Bartolozzi A,Seeberger PH

更新日期：2001-10-01 00:00:00

abstract：:Protein scientists are paving the way to a new phase in protein design and engineering. Approaches and methods are being developed that could allow the design of proteins beyond the confines of natural protein structures. This possibility of designing entirely new proteins opens new questions: What do we build? How do...

journal_title：Current opinion in structural biology

authors： Woolfson DN,Bartlett GJ,Burton AJ,Heal JW,Niitsu A,Thomson AR,Wood CW

更新日期：2015-08-01 00:00:00

abstract：:Computational methods play an important role at all stages of the process of determining protein-protein interactions. They are used to predict potential interactions, to validate the results of high-throughput interaction screens and to analyze the protein networks inferred from interaction databases. ...

journal_title：Current opinion in structural biology

authors： Salwinski L,Eisenberg D

更新日期：2003-06-01 00:00:00