Channel-tunnels.

abstract：:The accelerated elucidation of three-dimensional structures of protein complexes, both natural and designed, is providing new examples of large supramolecular assemblies with intriguing shapes. Those with high symmetry - based on the geometries of the Platonic solids - are particularly notable as their innately closed...

journal_title：Current opinion in structural biology

authors： Cannon KA,Ochoa JM,Yeates TO

更新日期：2019-04-01 00:00:00

abstract：:RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain comp...

journal_title：Current opinion in structural biology

authors： Mitchell D 3rd,Assmann SM,Bevilacqua PC

更新日期：2019-12-01 00:00:00

abstract：:CCA-adding enzymes (tRNA nucleotidyltransferases) are responsible for the maturation or repair of the functional 3' end of tRNAs. These enzymes are remarkable because they polymerize the essential nucleotides CCA onto the 3' terminus of tRNA precursors without using a nucleic acid template. Recent crystal structures, ...

journal_title：Current opinion in structural biology

authors： Xiong Y,Steitz TA

更新日期：2006-02-01 00:00:00

abstract：:The ribosome undergoes numerous large-scale conformational changes during protein synthesis, but the molecular basis for these changes have been unclear. Recent cryo-electron microscopic and X-ray crystallographic structures of both the bacterial and eukaryotic ribosome now provide snapshots of the wide range of motio...

journal_title：Current opinion in structural biology

authors： Noeske J,Cate JH

更新日期：2012-12-01 00:00:00

abstract：:In the past few years, structural biology has begun to reveal details of some of the receptors and associated interactions responsible for protein-mediated recognition events at the cell surface. Recent data span the fields of cytokine-receptor interactions, cell adhesion molecules and viral infection. ...

journal_title：Current opinion in structural biology

authors： Stuart DI,Jones EY

更新日期：1995-12-01 00:00:00

abstract：:Fundamental biological processes are driven by diverse molecular machineries. In recent years, single-molecule fluorescence spectroscopy has matured as a unique tool in biology to study how structural dynamics of molecular complexes drive various biochemical reactions. In this review, we highlight underlying developme...

journal_title：Current opinion in structural biology

authors： Choi J,Grosely R,Puglisi EV,Puglisi JD

更新日期：2019-10-01 00:00:00

abstract：:The ClC proteins are members of a large family of chloride transport proteins, which are involved in a variety of physiological processes. All family members share a conserved molecular architecture consisting of a complex transmembrane transport domain and a soluble regulatory domain. To date, representative structur...

journal_title：Current opinion in structural biology

authors： Dutzler R

更新日期：2006-08-01 00:00:00

abstract：:Current research into the morphology of lipid membranes focuses on three areas: first, the transformations and fluctuations of the shape of freely suspended vesicles; second, the morphology of membranes that experience mutual interactions or external forces arising, for example, from a macroscopic surface or from opti...

journal_title：Current opinion in structural biology

authors： Lipowsky R

更新日期：1995-08-01 00:00:00

abstract：:Cohesin-Dockerin interactions are at the core of cellulosomal assembly and organization. They are highly specific and form stable complexes, allowing cellulosomes to adopt distinct conformations. Each cellulosomal system seems to have a particular organizational strategy that can vary in complexity according to the na...

journal_title：Current opinion in structural biology

authors： Bule P,Pires VM,Fontes CM,Alves VD

更新日期：2018-04-01 00:00:00

abstract：:The anaphase-promoting complex/cyclosome (APC/C) is a large multi-subunit complex that functions as a RING domain E3 ubiquitin ligase to regulate transitions through the cell cycle, achieved by controlling the defined ubiquitin-dependent degradation of specific cell cycle regulators. APC/C activity and substrate selec...

journal_title：Current opinion in structural biology

authors： Barford D

更新日期：2020-04-01 00:00:00

abstract：:The past decade has seen a revolution in our ability to engineer designer enzymes using genetic tools that mimic evolution on a laboratory timescale. Many excellent examples of directed evolution applied to a wide range of enzymes have clearly demonstrated its future role in adapting enzymes for use in the chemical in...

journal_title：Current opinion in structural biology

authors： Dalby PA

更新日期：2003-08-01 00:00:00

abstract：:Magic-angle spinning solid-state NMR experiments are well suited to investigating the structures and mechanisms of important proteins that are inaccessible to X-ray crystallography and solution NMR spectroscopy, including membrane proteins and disease-related protein aggregates. Good progress has been made in the deve...

journal_title：Current opinion in structural biology

authors： Thompson LK

更新日期：2002-10-01 00:00:00

abstract：:During the past few years, there have been exciting developments in the field of flavoenzymology. New flavoenzymes have been discovered that are implicated in a variety of biological processes, including cell signaling, chromatin remodeling and cell development. The structures of several of these new flavoenzymes have...

journal_title：Current opinion in structural biology

authors： De Colibus L,Mattevi A

更新日期：2006-12-01 00:00:00

abstract：:New studies have shown that folding of beta-sheet proteins can occur with and without intermediates, with fast to slow refolding rates and late to very late transition states. These experiments demonstrate that, despite early speculation to the contrary, beta-sheet protein folding does not appear to be fundamentally d...

journal_title：Current opinion in structural biology

authors： Capaldi AP,Radford SE

更新日期：1998-02-01 00:00:00

abstract：:Toll-like receptors (TLRs) activate the innate immune system in response to invading pathogens. Although nucleic acids are one of the principal TLR ligands, they are not inherently pathogen-specific and, thus, carry the risk of triggering autoimmunity. There are multiple unique regulatory mechanisms aimed at preventin...

journal_title：Current opinion in structural biology

authors： Shimizu T

更新日期：2017-12-01 00:00:00

abstract：:Zinc finger proteins are among the most abundant proteins in eukaryotic genomes. Their functions are extraordinarily diverse and include DNA recognition, RNA packaging, transcriptional activation, regulation of apoptosis, protein folding and assembly, and lipid binding. Zinc finger structures are as diverse as their f...

journal_title：Current opinion in structural biology

authors： Laity JH,Lee BM,Wright PE

更新日期：2001-02-01 00:00:00

abstract：:Malaria remains the world's most prevalent human parasitic disease. Because of the rapid spread of drug resistance in parasites, there is an urgent need to identify diverse new drug targets. One group of proteases that are emerging as targets for novel antimalarials are the metalloaminopeptidases. These enzymes cataly...

journal_title：Current opinion in structural biology

authors： McGowan S

更新日期：2013-12-01 00:00:00

abstract：:For many protein families, such as serine proteinases or serine proteinase inhibitors, the family assignment predicts reactivity only in general terms. Both detailed specificity and quantitative reactivity are lacking. We believe that, for many such protein families, algorithms can be devised by defining the subset of...

journal_title：Current opinion in structural biology

authors： Laskowski M Jr,Qasim MA,Yi Z

更新日期：2003-02-01 00:00:00

abstract：:Polysaccharide monooxygenases, or PMOs (also known as lytic PMOs or LPMOs), are a group of enzymes discovered in recent years to catalyze the oxidative degradation of carbohydrate polymers. The PMO catalytic domain has a β-sandwich fold that bears a strong resemblance to both immunoglobulin (Ig) and fibronectin type I...

journal_title：Current opinion in structural biology

authors： Span EA,Marletta MA

更新日期：2015-12-01 00:00:00

abstract：:Membranes are sites of intense signaling activity within the cell, serving as dynamic scaffolds for the recruitment of signaling molecules and their substrates. The specific and reversible localization of these signaling molecules to membranes is critical for the appropriate activation of downstream signaling pathways...

journal_title：Current opinion in structural biology

authors： Leonard TA,Hurley JH

更新日期：2011-12-01 00:00:00

abstract：:Recent advances in neutron and X-ray sources and instrumentation, new and improved scattering techniques, and molecular biology techniques, which have permitted facile preparation of samples, have each led to new opportunities in using small-angle scattering to study the conformations and interactions of biological ma...

journal_title：Current opinion in structural biology

authors： Trewhella J

更新日期：1997-10-01 00:00:00

abstract：:Kinetochores are large macromolecular assemblies that link chromosomes to spindle microtubules (MTs) during mitosis. Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vitro and nanometer-accuracy fluorescence microscopy in vivo. We synthesize these...

journal_title：Current opinion in structural biology

authors： Alushin G,Nogales E

更新日期：2011-10-01 00:00:00

abstract：:Eukaryotic transcriptional coactivators are multi-subunit complexes that both modify chromatin and recognize histone modifications. Until recently, structural information on these large complexes has been limited to isolated enzymatic domains or chromatin-binding motifs. This review summarizes recent structural studie...

journal_title：Current opinion in structural biology

authors： Samara NL,Wolberger C

更新日期：2011-12-01 00:00:00

abstract：:Alternatively spliced protein segments tend to be intrinsically disordered and contain linear interaction motifs and/or post-translational modification sites. An emerging concept is that differential inclusion of such disordered segments can mediate new protein interactions, and hence change the context in which the b...

journal_title：Current opinion in structural biology

authors： Buljan M,Chalancon G,Dunker AK,Bateman A,Balaji S,Fuxreiter M,Babu MM

更新日期：2013-06-01 00:00:00

abstract：:High-throughput interaction discovery initiatives have revealed the existence of hundreds of multiprotein complexes whose functions are regulated through thousands of protein-protein interactions (PPIs). However, the structural details of these interactions, often necessary to understand their function, are only avail...

journal_title：Current opinion in structural biology

authors： Stein A,Mosca R,Aloy P

更新日期：2011-04-01 00:00:00

abstract：:X-ray radiation damage to cryocooled ( approximately 100 K) macromolecular crystals has emerged as a general problem, especially since the advent of third generation synchrotron undulator sources. Interest in understanding the physical and chemical phenomena behind the observed effects is growing rapidly. The specific...

journal_title：Current opinion in structural biology

authors： Ravelli RB,Garman EF

更新日期：2006-10-01 00:00:00

abstract：:Significant progress has been made over the past several years on structural studies of the eukaryotic translation initiation factors that facilitate the assembly of a translation-competent ribosome at the initiation codon of an mRNA. These structural studies have revealed the repeated use of a set of common structura...

journal_title：Current opinion in structural biology

authors： Sonenberg N,Dever TE

更新日期：2003-02-01 00:00:00

abstract：:Electron cryo-microscopy (cryoEM) is used to determine structures of biological molecules, including multi-protein complexes. Maps at better than 3.0Å resolution are relatively straightforward to interpret since atomic models of proteins and nucleic acids can be built directly. Still, these resolutions are often diffi...

journal_title：Current opinion in structural biology

authors： Casañal A,Shakeel S,Passmore LA

更新日期：2019-10-01 00:00:00

abstract：:Small-angle X-ray scattering (SAXS) has emerged as an enabling integrative technique for comprehensive analyses of macromolecular structures and interactions in solution. Over the past two decades, SAXS has become a mainstay of the structural biologist's toolbox, supplying multiplexed measurements of molecular shape a...

journal_title：Current opinion in structural biology

authors： Brosey CA,Tainer JA

更新日期：2019-10-01 00:00:00

abstract：:There are now many successful examples of the design of new ligands based on knowledge of target protein structures. In most cases those ligands are unsuitable as drugs because of problems of toxicity, stability or bioavailability. The past twelve months have also seen the description of the structures of many protein...

journal_title：Current opinion in structural biology

authors： Colman PM

更新日期：1994-12-01 00:00:00