Abstract:
:RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain complete structuromes, and are applicable to multiple domains of life and conditions. New reagents such as 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), glyoxal, and nicotinoyl azide (NAz) greatly expand the capabilities of nucleobase probing in cells. Additionally, ribose-targeting reagents in selective 2'-hydroxyl acylation and primer extension (SHAPE) detect RNA flexibility in vivo. These techniques, coupled with crosslinking nucleobases in psoralen analysis of RNA interactions and structures (PARIS), provide new and diverse ways to elucidate RNA secondary and tertiary structure in vivo and genome-wide.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Mitchell D 3rd,Assmann SM,Bevilacqua PCdoi
10.1016/j.sbi.2019.07.008subject
Has Abstractpub_date
2019-12-01 00:00:00pages
151-158eissn
0959-440Xissn
1879-033Xpii
S0959-440X(18)30217-3journal_volume
59pub_type
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