Promiscuity, impersonation and accommodation: evolution of plant specialized metabolism.

abstract：:Covalently circularized nanodiscs (cNDs) represent a significant advance in the durability and applicability of nanodisc technology. The new cNDs demonstrate higher size homogeneity and improved stability compared with that of non-circularized forms. Moreover, cNDs can be prepared at various defined sizes up to 80-nm ...

journal_title：Current opinion in structural biology

authors： Nasr ML,Wagner G

更新日期：2018-08-01 00:00:00

abstract：:2D-IR spectroscopy has matured to a powerful technique to study the structure and dynamics of peptides, but its extension to larger proteins is still in its infancy, the major limitations being sensitivity and selectivity. Site-selective information requires measuring single vibrational probes at sub-millimolar concen...

journal_title：Current opinion in structural biology

authors： Koziol KL,Johnson PJ,Stucki-Buchli B,Waldauer SA,Hamm P

更新日期：2015-10-01 00:00:00

abstract：:DNA photolyases are extremely efficient light-driven DNA repair enzymes that use the energy of a blue-light photon to 'inject' an electron onto UV-damaged DNA, catalyzing the splitting of mutagenic pyrimidine dimers. By contrast, cryptochromes use blue light to trigger signaling cascades in multicellular organisms, fu...

journal_title：Current opinion in structural biology

authors： Essen LO

更新日期：2006-02-01 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are increasingly realized to play diverse biological roles, ranging from molecular signaling to the formation of membraneless organelles. Their high degree of disorder makes them more challenging to study using the techniques of conventional structural biology, because any obse...

journal_title：Current opinion in structural biology

authors： Best RB

更新日期：2017-02-01 00:00:00

abstract：:Structural modeling of a cell is an evolving strategic direction in computational structural biology. It takes advantage of new powerful modeling techniques, deeper understanding of fundamental principles of molecular structure and assembly, and rapid growth of the amount of structural data generated by experimental t...

journal_title：Current opinion in structural biology

authors： Vakser IA,Deeds EJ

更新日期：2019-04-01 00:00:00

abstract：:Ribosomes translate genetic information encoded by mRNAs into protein chains with high fidelity. Truncated mRNAs lacking a stop codon will cause the synthesis of incomplete peptide chains and stall translating ribosomes. In bacteria, a ribonucleoprotein complex composed of tmRNA, a molecule that combines the functions...

journal_title：Current opinion in structural biology

authors： Haebel PW,Gutmann S,Ban N

更新日期：2004-02-01 00:00:00

abstract：:The torque-generating, direction-reversing switch proteins of the bacterial flagellar rotary motor form a cytoplasmic extension of the bacterial flagellar basal body. 10 A maps, obtained by electron cryomicroscopy, of the bacterial filament reveal an unusual alpha domain which forms the protein-subunit export channel....

journal_title：Current opinion in structural biology

authors： DeRosier DJ

更新日期：1995-04-01 00:00:00

abstract：:Historically it has been virtually impossible to experimentally determine the contribution of residual protein entropy to fundamental protein activities such as the binding of ligands. Recent progress has illuminated the possibility of employing NMR relaxation methods to quantitatively determine the role of changes in...

journal_title：Current opinion in structural biology

authors： Wand AJ

更新日期：2013-02-01 00:00:00

abstract：:Connecting three dimensional structure and affinity is analogous to seeking the 'Archimedean point', a vantage point from where any observer can quantitatively perceive the subject of inquiry. Here we review current knowledge and challenges that lie ahead of us in the quest for this Archimedean point. We argue that cu...

journal_title：Current opinion in structural biology

authors： Kastritis PL,Bonvin AM

更新日期：2013-12-01 00:00:00

abstract：:In all contemporary organisms, the active site of the ribosome--the peptidyl transferase center--catalyzes two distinct reactions, peptide bond formation between peptidyl-tRNA and aminoacyl-tRNA as well as the hydrolysis of peptidyl-tRNA with the help of a release factor. However, when provided with appropriate substr...

journal_title：Current opinion in structural biology

authors： Rodnina MV

更新日期：2013-08-01 00:00:00

abstract：:Eukaryotic mRNAs contain a 5' cap structure that protects the transcript against rapid exonucleolytic degradation. The regulation of cellular mRNA levels therefore depends on a precise control of the mRNA decapping pathways. The major mRNA decapping enzyme in eukaryotic cells is Dcp2. It is regulated by interactions w...

journal_title：Current opinion in structural biology

authors： Wurm JP,Sprangers R

更新日期：2019-12-01 00:00:00

abstract：:Membranes are sites of intense signaling activity within the cell, serving as dynamic scaffolds for the recruitment of signaling molecules and their substrates. The specific and reversible localization of these signaling molecules to membranes is critical for the appropriate activation of downstream signaling pathways...

journal_title：Current opinion in structural biology

authors： Leonard TA,Hurley JH

更新日期：2011-12-01 00:00:00

abstract：:Small-angle X-ray scattering (SAXS) has emerged as an enabling integrative technique for comprehensive analyses of macromolecular structures and interactions in solution. Over the past two decades, SAXS has become a mainstay of the structural biologist's toolbox, supplying multiplexed measurements of molecular shape a...

journal_title：Current opinion in structural biology

authors： Brosey CA,Tainer JA

更新日期：2019-10-01 00:00:00

abstract：:The past years have seen tremendous progress towards understanding how arrestins recognize phosphorylated G protein-coupled receptors (GPCRs). Two arrestin crystal structures, one of a pre-activated splice variant and one bound to a GPCR phosphopeptide, provided insights into the conformational changes upon phosphate ...

journal_title：Current opinion in structural biology

authors： Ostermaier MK,Schertler GF,Standfuss J

更新日期：2014-12-01 00:00:00

abstract：:The ClC proteins are members of a large family of chloride transport proteins, which are involved in a variety of physiological processes. All family members share a conserved molecular architecture consisting of a complex transmembrane transport domain and a soluble regulatory domain. To date, representative structur...

journal_title：Current opinion in structural biology

authors： Dutzler R

更新日期：2006-08-01 00:00:00

abstract：:Intrinsically disordered regions (IDRs) are fundamental units of protein function and regulation. Despite their inability to form a unique stable tertiary structure in isolation, many IDRs adopt a defined conformation upon binding and achieve their function through their interactions with other biomolecules. However, ...

journal_title：Current opinion in structural biology

authors： Flock T,Weatheritt RJ,Latysheva NS,Babu MM

更新日期：2014-06-01 00:00:00

abstract：:The past decade has witnessed the development of numerous systems for the presentation of antigens on the surface of self-assembling macromolecules. Although the sites for insertion were initially chosen empirically, the determination of the three-dimensional structures of a number of carrier macromolecules has enable...

journal_title：Current opinion in structural biology

authors： Lomonossoff GP,Johnson JE

更新日期：1996-04-01 00:00:00

abstract：:C-type lectins are the largest and most diverse family of mammalian carbohydrate-binding proteins. They share a common protein fold, which provides the unifying basis for calcium-mediated carbohydrate recognition. Their involvement in a multitude of biological functions is remarkable. Here, we review the variety of ta...

journal_title：Current opinion in structural biology

authors： Keller BG,Rademacher C

更新日期：2020-06-01 00:00:00

abstract：:The polyadenosine (poly(A)) tail found on the 3'-end of almost all eukaryotic mRNAs is important for mRNA stability and regulation of translation. mRNA 3'-end processing occurs co-transcriptionally and involves more than 20 proteins to specifically recognize the polyadenylation site, cleave the pre-mRNA, add a poly(A)...

journal_title：Current opinion in structural biology

authors： Kumar A,Clerici M,Muckenfuss LM,Passmore LA,Jinek M

更新日期：2019-12-01 00:00:00

abstract：:Cells are equipped with cosolvents that modulate protein folding and aggregation to withstand water stress. The effect of cosolvents on the aggregation rates depends on whether the polypeptide sequence is an intrinsically disordered protein (IDP) or can fold into a specific native structure. Cosolvents, which act as d...

journal_title：Current opinion in structural biology

authors： Reddy G,Muttathukattil AN,Mondal B

更新日期：2020-02-01 00:00:00

abstract：:You want to know how proteins do it? Take a walk in protein fold space. More often than not you will get a clue if not the answer. If you know what you are looking for and how to find it. In fact, there is more information than we can presently handle. Charting fold space and chasing its creatures has occupied us for ...

journal_title：Current opinion in structural biology

authors： Sippl MJ

更新日期：2009-06-01 00:00:00

abstract：:Ribosome recycling usually occurs after canonical termination triggered by a stop codon. Additionally, ribosomes that are stalled by aberrant mRNAs need to be recognized and subsequently recycled. In eukaryotes and archaea, the factors involved in canonical termination and ribosome rescue are structurally and function...

journal_title：Current opinion in structural biology

authors： Franckenberg S,Becker T,Beckmann R

更新日期：2012-12-01 00:00:00

abstract：:The past twelve months have seen a renewal of interest in the therapeutic potential of members of the tumour necrosis factor receptor family and their cytokine ligands. This biomedical interest has spawned a number of structural studies, which have significantly deepened our understanding of the molecular basis for th...

journal_title：Current opinion in structural biology

authors： Jones EY

更新日期：2000-12-01 00:00:00

abstract：:Alternatively spliced protein segments tend to be intrinsically disordered and contain linear interaction motifs and/or post-translational modification sites. An emerging concept is that differential inclusion of such disordered segments can mediate new protein interactions, and hence change the context in which the b...

journal_title：Current opinion in structural biology

authors： Buljan M,Chalancon G,Dunker AK,Bateman A,Balaji S,Fuxreiter M,Babu MM

更新日期：2013-06-01 00:00:00

abstract：:If protein structure prediction methods are to make any impact on the impending onerous task of analyzing the large numbers of unknown protein sequences generated by the ongoing genome-sequencing projects, it is vital that they make the difficult transition from computational 'gedankenexperiments' to practical softwar...

journal_title：Current opinion in structural biology

authors： Jones DT

更新日期：1997-06-01 00:00:00

abstract：:Molecular dynamics simulations allow for atomic-level characterization of biomolecular processes such as the conformational transitions associated with protein function. The computational demands of such simulations, however, have historically prevented them from reaching the microsecond and greater timescales on whic...

journal_title：Current opinion in structural biology

authors： Klepeis JL,Lindorff-Larsen K,Dror RO,Shaw DE

更新日期：2009-04-01 00:00:00

abstract：:Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technol...

journal_title：Current opinion in structural biology

authors： Mesa P,Deniaud A,Montoya G,Schaffitzel C

更新日期：2013-06-01 00:00:00

abstract：:Advances in the crystallization of biological macromolecules have come not only from the application of biochemical, molecular biological and immunological principles and techniques, but also from continued efforts to understand the crystallization process. Developments in crystallization methodologies, protocols, and...

journal_title：Current opinion in structural biology

authors： Gilliland GL,Ladner JE

更新日期：1996-10-01 00:00:00

abstract：:A novel ligand-binding domain, named the 'ACT domain', was recently identified by a PSI-BLAST search. The archetypical ACT domain is the C-terminal regulatory domain of 3-phosphoglycerate dehydrogenase (3PGDH), which folds with a ferredoxin-like betaalphabetabetaalphabeta topology. A pair of ACT domains form an eight-...

journal_title：Current opinion in structural biology

authors： Chipman DM,Shaanan B

更新日期：2001-12-01 00:00:00

abstract：:ATP- and NAD(+)-dependent DNA ligases, ATP-dependent RNA ligases and GTP-dependent mRNA capping enzymes comprise a superfamily of proteins that catalyze nucleotidyl transfer to polynucleotide 5' ends via covalent enzyme-(lysyl-N)-NMP intermediates. The superfamily is defined by five peptide motifs that line the nucleo...

journal_title：Current opinion in structural biology

authors： Shuman S,Lima CD

更新日期：2004-12-01 00:00:00