Covalently circularized nanodiscs; challenges and applications.

abstract：:RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain comp...

journal_title：Current opinion in structural biology

authors： Mitchell D 3rd,Assmann SM,Bevilacqua PC

更新日期：2019-12-01 00:00:00

abstract：:The observed distribution of protein structures can give us important clues about the underlying evolutionary process, imposing important constraints on possible models. The availability of results from an increasing number of genome projects has made the development of these models an active area of research. Models ...

journal_title：Current opinion in structural biology

authors： Goldstein RA

更新日期：2008-04-01 00:00:00

abstract：:The structure of the visual pigment rhodopsin in the dark state was first investigated by electron microscopy (EM). More recently, rhodopsin has been crystallised in two different space groups--a tetragonal P4(1) crystal form and a trigonal P3(1) packing arrangement. The structures of the pigment, determined by X-ray ...

journal_title：Current opinion in structural biology

authors： Schertler GF

更新日期：2005-08-01 00:00:00

abstract：:Transition states in protein folding are being analyzed experimentally and by computer simulation to reveal the sequence of events in the folding and unfolding of proteins at high resolution. Results of this analysis have enabled the reconstruction of folding pathways in vitro and open the prospect of analyzing pathwa...

journal_title：Current opinion in structural biology

authors： Fersht AR

更新日期：1995-02-01 00:00:00

abstract：:The wide occurrence of sialic acids (Sia) in various chemical forms linked as monomers or polymers in an outstanding position in a multitude of complex carbohydrates of animals and microorganisms renders them as most versatile function modulators in cell biology and pathology. A survey is presented of recent advances ...

journal_title：Current opinion in structural biology

authors： Schauer R

更新日期：2009-10-01 00:00:00

abstract：:Congenital disorders of glycosylation (CDG) are a group of diseases that affect glycoprotein biogenesis. Eighteen different types of CDG have been defined genetically. They result from deficiencies in either the biosynthesis of oligosaccharide precursors or specific steps of N-glycan assembly, resulting in the absence...

journal_title：Current opinion in structural biology

authors： Freeze HH,Aebi M

更新日期：2005-10-01 00:00:00

abstract：:Salt-dependent electrostatic effects are a major factor in determining the stability, structure, reactivity, and binding behavior of nucleic acids. Increasingly detailed theoretical methods, especially those based on Monte Carlo and Poisson-Boltzmann methodologies, combined with powerful computational algorithms are b...

journal_title：Current opinion in structural biology

authors： Sharp KA,Honig B

更新日期：1995-06-01 00:00:00

abstract：:The annotation of protein function at genomic scale is essential for day-to-day work in biology and for any systematic approach to the modeling of biological systems. Currently, functional annotation is essentially based on the expansion of the relatively small number of experimentally determined functions to large co...

journal_title：Current opinion in structural biology

authors： Valencia A

更新日期：2005-06-01 00:00:00

abstract：:The past twelve months have seen a renewal of interest in the therapeutic potential of members of the tumour necrosis factor receptor family and their cytokine ligands. This biomedical interest has spawned a number of structural studies, which have significantly deepened our understanding of the molecular basis for th...

journal_title：Current opinion in structural biology

authors： Jones EY

更新日期：2000-12-01 00:00:00

abstract：:The past year has witnessed a tremendous increase in our understanding of the structures and interactions of the GTPases. The highlights include crystal structures of G alpha subunits, as well as the first complex between a GTPase (Rap1A) and an effector molecule (c-Raf1 Ras-binding domain). In the field of elongation...

journal_title：Current opinion in structural biology

authors： Hilgenfeld R

更新日期：1995-12-01 00:00:00

abstract：:Protein-protein interactions involving the catalytic domain of protein kinases are likely to be generally important in the regulation of signal transduction pathways, but are rather sparsely represented in crystal structures. Recently determined structures of the kinase domains of the mitogen-activated protein kinase ...

journal_title：Current opinion in structural biology

authors： Pellicena P,Kuriyan J

更新日期：2006-12-01 00:00:00

abstract：:Akt1-3 (Akt) are a subset of the AGC protein Ser/Thr kinase family and play important roles in cell growth, metabolic regulation, cancer, and other diseases. We describe some of the roles of Akt in cell signaling and the biochemical and structural mechanisms of the regulation of Akt catalysis by the phospholipid PIP3 ...

journal_title：Current opinion in structural biology

authors： Cole PA,Chu N,Salguero AL,Bae H

更新日期：2019-12-01 00:00:00

abstract：:Ring-shaped nucleic acid translocases and helicases catalyze the directed and processive movement of nucleic acid strands to support essential transactions such as replication, transcription, and chromosome partitioning. Assembled typically as hexamers, ring helicase/translocase systems use coordinated cycles of nucle...

journal_title：Current opinion in structural biology

authors： O'Shea VL,Berger JM

更新日期：2014-04-01 00:00:00

abstract：:Ising-like models have been remarkably successful in reproducing all the experimental data available on the equilibrium and kinetics of secondary structure formation in short peptides. Over the past two years, very similar models have been used to predict the folding of complete proteins, with encouraging results. Alt...

journal_title：Current opinion in structural biology

authors： Muñoz V

更新日期：2001-04-01 00:00:00

abstract：:Ribosomes translate genetic information encoded by mRNAs into protein chains with high fidelity. Truncated mRNAs lacking a stop codon will cause the synthesis of incomplete peptide chains and stall translating ribosomes. In bacteria, a ribonucleoprotein complex composed of tmRNA, a molecule that combines the functions...

journal_title：Current opinion in structural biology

authors： Haebel PW,Gutmann S,Ban N

更新日期：2004-02-01 00:00:00

abstract：:Advances in electron microscopes, detectors and data processing algorithms have greatly facilitated the structural determination of many challenging integral membrane proteins that have been evasive to crystallization. These breakthroughs facilitate the application and development of various membrane protein solubiliz...

journal_title：Current opinion in structural biology

authors： Autzen HE,Julius D,Cheng Y

更新日期：2019-10-01 00:00:00

abstract：:RNA motifs are directed and ordered stacked arrays of non-Watson-Crick base pairs forming distinctive foldings of the phosphodiester backbones of the interacting RNA strands. They correspond to the 'loops' - hairpin, internal and junction - that intersperse the Watson-Crick two-dimensional helices as seen in two-dimen...

journal_title：Current opinion in structural biology

authors： Leontis NB,Westhof E

更新日期：2003-06-01 00:00:00

abstract：:Fundamental biological processes are driven by diverse molecular machineries. In recent years, single-molecule fluorescence spectroscopy has matured as a unique tool in biology to study how structural dynamics of molecular complexes drive various biochemical reactions. In this review, we highlight underlying developme...

journal_title：Current opinion in structural biology

authors： Choi J,Grosely R,Puglisi EV,Puglisi JD

更新日期：2019-10-01 00:00:00

abstract：:Connecting three dimensional structure and affinity is analogous to seeking the 'Archimedean point', a vantage point from where any observer can quantitatively perceive the subject of inquiry. Here we review current knowledge and challenges that lie ahead of us in the quest for this Archimedean point. We argue that cu...

journal_title：Current opinion in structural biology

authors： Kastritis PL,Bonvin AM

更新日期：2013-12-01 00:00:00

abstract：:Recent studies have revealed a correlation between amino acid sequences around glycosylation sites in proteoglycans and the ability of cells to initiate and process glycosaminoglycan chains. Initiation depends on Ser-Gly/Ala dipeptides that have one or more acidic amino acids in close proximity. The formation of hepar...

journal_title：Current opinion in structural biology

authors： Esko JD,Zhang L

更新日期：1996-10-01 00:00:00

abstract：:Continuing investigations into protein-protein interactions have revealed their key role in regulating a wide range of cellular processes. Although efforts to modulate these interactions are more challenging and much less mature than work on conventional drug discovery pathways, significant progress has been made on s...

journal_title：Current opinion in structural biology

authors： Zhao L,Chmielewski J

更新日期：2005-02-01 00:00:00

abstract：:Among the crystal structures of lectins determined recently, three--snowdrop lectin, jacalin and amaranthin--represent new lectin families. Their polypeptide folds share remarkably similar features and consist exclusively of beta structure. Autonomously folded beta-sheet subdomains, inter-related by a pseudothreefold ...

journal_title：Current opinion in structural biology

authors： Wright CS

更新日期：1997-10-01 00:00:00

abstract：:The radical intermediates generated during the catalytic cycles of adenosylcobalamin-dependent enzymes occur in pairs. The positions of radicals residing on the cofactor, substrate or protein, relative to the position of the low-spin Co(2+) from the cob(II)alamin intermediate, can be extracted from electron paramagnet...

journal_title：Current opinion in structural biology

authors： Reed GH,Mansoorabadi SO

更新日期：2003-12-01 00:00:00

abstract：:Mechanisms for making and breaking the heme b cofactor (heme) are more diverse than previously expected. Biosynthetic pathways have diverged at least twice along taxonomic lines, reflecting differences in membrane organization and O2 utilization among major groups of organisms. At least three families of heme degradas...

journal_title：Current opinion in structural biology

authors： Celis AI,DuBois JL

更新日期：2019-12-01 00:00:00

abstract：:Self-compartmentalizing proteases, such as the proteasome and several prokaryotic energy-dependent proteases, are designed to act in the crowded environment of the cell. Proteins destined for degradation are recognized and unfolded by regulatory subcomplexes that invariably contain ATPase modules, before being translo...

journal_title：Current opinion in structural biology

authors： Zwickl P,Baumeister W,Steven A

更新日期：2000-04-01 00:00:00

abstract：:Dynamin is an important component of membrane recycling at the plasma membrane and, potentially, within the cell. The role of dynamin in clathrin-mediated endocytosis has been based on numerous endocytosis assays, as well as on the discovery and gross characterization of the assembled spiral structure of dynamin. Rece...

journal_title：Current opinion in structural biology

authors： Hinshaw JE

更新日期：1999-04-01 00:00:00

abstract：:Antibody engineering has been an extremely intensive research area for many years. Recent achievements discussed in this review include: (i) significant improvements in the field of selection of antigen-specific antibody fragments on bacteriophages; (ii) new structural work, in particular using NMR; (iii) the cloning ...

journal_title：Current opinion in structural biology

authors： Nilsson B

更新日期：1995-08-01 00:00:00

abstract：:Transmembrane proton gradients, known as Proton Motive Force (PMF), serve important physiological functions. In addition to driving ATP synthesis, PMF is harnessed by a large variety of secondary transporters to achieve 'uphill' translocation of specific substrates across the membrane. Proton-coupled secondary transpo...

journal_title：Current opinion in structural biology

authors： Yan N

更新日期：2013-08-01 00:00:00

abstract：:An important goal of structural genomics is to complete the structural analysis of all the enzymes in metabolic pathways and to understand the structural similarities and differences. A preliminary glimpse of this type of analysis was achieved before structural genomics efforts with the glycolytic pathway and efforts ...

journal_title：Current opinion in structural biology

authors： Erlandsen H,Abola EE,Stevens RC

更新日期：2000-12-01 00:00:00

abstract：:Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technol...

journal_title：Current opinion in structural biology

authors： Mesa P,Deniaud A,Montoya G,Schaffitzel C

更新日期：2013-06-01 00:00:00