New folds of plant lectins.

abstract：:Recent years have seen a rapid increase in structural information on proteins implicated in bacterial pathogenesis. The different modes by which bacteria establish contact with their host tissues are exemplified by the structures of bacterial adhesins in complex with their cognate host receptor. A more detailed struct...

journal_title：Current opinion in structural biology

authors： Remaut H,Waksman G

更新日期：2004-04-01 00:00:00

abstract：:There has been significant recent progress in the computational design of protein interactions including the creation of novel heterodimers, homodimers, nanohedra, fibril caps and a protein crystal. Essential to these successes has been the use of innovative strategies for finding binding modes that are achievable, th...

journal_title：Current opinion in structural biology

authors： Der BS,Kuhlman B

更新日期：2013-08-01 00:00:00

abstract：:Newly synthesized proteins leave the ribosome through a narrow tunnel in the large subunit. During ongoing synthesis, nascent protein chains are particularly sensitive to aggregation and degradation because they emerge from the ribosome in an unfolded state. In bacteria, the first protein to interact with nascent chai...

journal_title：Current opinion in structural biology

authors： Maier T,Ferbitz L,Deuerling E,Ban N

更新日期：2005-04-01 00:00:00

abstract：:NMR relaxation experiments of isotopically labeled proteins provide a wealth of information on reorientational global and local dynamics on nanosecond and subnanosecond timescales for folded and nonfolded proteins in solution. Recent methodological advances in the interpretation of relaxation data have led to a better...

journal_title：Current opinion in structural biology

authors： Brüschweiler R

更新日期：2003-04-01 00:00:00

abstract：:Covalent modifications of histone proteins regulate a wide variety of cellular processes. Methylation of histone H3K79 and H3K4 is associated with active transcription and is catalyzed by Dot1L and Set1, respectively. Both Dot1L and Set1 are activated by prior ubiquitination of histone H2B on K120 in a process termed ...

journal_title：Current opinion in structural biology

authors： Worden EJ,Wolberger C

更新日期：2019-12-01 00:00:00

abstract：:The past decade has witnessed the development of numerous systems for the presentation of antigens on the surface of self-assembling macromolecules. Although the sites for insertion were initially chosen empirically, the determination of the three-dimensional structures of a number of carrier macromolecules has enable...

journal_title：Current opinion in structural biology

authors： Lomonossoff GP,Johnson JE

更新日期：1996-04-01 00:00:00

abstract：:The encounter complex of two proteins is a dynamic intermediate state that guides proteins to their binding site, thus enhancing the rate of complex formation. It is particularly useful for complexes that must balance a biological requirement for high turnover with the need for specific binding, such as electron trans...

journal_title：Current opinion in structural biology

authors： Schilder J,Ubbink M

更新日期：2013-12-01 00:00:00

abstract：:The spliceosome executes eukaryotic precursor messenger RNA (pre-mRNA) splicing to remove noncoding introns through two sequential transesterification reactions, branching and exon ligation. The fidelity of this process is based on the recognition of the conserved sequences in the intron and dynamic compositional and ...

journal_title：Current opinion in structural biology

authors： Wan R,Bai R,Shi Y

更新日期：2019-12-01 00:00:00

abstract：:Eukaryotes possess a variety of translational control mechanisms which function in the surveillance of mRNAs, discriminating between normal and aberrant translation elongation and termination, triggering mRNA decay. The three major evolutionarily conserved eukaryotic pathways are No-Go, Non-Stop and Nonsense-Mediated ...

journal_title：Current opinion in structural biology

authors： Powers KT,Szeto JA,Schaffitzel C

更新日期：2020-12-01 00:00:00

abstract：:High-throughput interaction discovery initiatives have revealed the existence of hundreds of multiprotein complexes whose functions are regulated through thousands of protein-protein interactions (PPIs). However, the structural details of these interactions, often necessary to understand their function, are only avail...

journal_title：Current opinion in structural biology

authors： Stein A,Mosca R,Aloy P

更新日期：2011-04-01 00:00:00

abstract：:Almost two decades after their identification, the components of the mitotic checkpoint are finally revealing their structural secrets. The activation of Mad2, a central piece of the checkpoint protein machinery, is linked to the rare ability of this protein to adopt two distinct topologies. Current models of checkpoi...

journal_title：Current opinion in structural biology

authors： Mapelli M,Musacchio A

更新日期：2007-12-01 00:00:00

abstract：:Given the increasing interest in protein-protein interactions, the prediction of these interactions from sequence and structural information has become a booming activity. CAPRI, the community-wide experiment for assessing blind predictions of protein-protein interactions, is playing an important role in fostering pro...

journal_title：Current opinion in structural biology

authors： Wodak SJ,Méndez R

更新日期：2004-04-01 00:00:00

abstract：:The torque-generating, direction-reversing switch proteins of the bacterial flagellar rotary motor form a cytoplasmic extension of the bacterial flagellar basal body. 10 A maps, obtained by electron cryomicroscopy, of the bacterial filament reveal an unusual alpha domain which forms the protein-subunit export channel....

journal_title：Current opinion in structural biology

authors： DeRosier DJ

更新日期：1995-04-01 00:00:00

abstract：:Membrane protein structural biology is enjoying a steady acceleration in the rate of success. Nevertheless, numerous membrane protein targets are resistant to the traditional approach of directly crystallizing detergent solubilized and purified protein and the 'niche market' of lipidic phase crystallization is emergin...

journal_title：Current opinion in structural biology

authors： Johansson LC,Wöhri AB,Katona G,Engström S,Neutze R

更新日期：2009-08-01 00:00:00

abstract：:Molecular dynamics simulations allow for atomic-level characterization of biomolecular processes such as the conformational transitions associated with protein function. The computational demands of such simulations, however, have historically prevented them from reaching the microsecond and greater timescales on whic...

journal_title：Current opinion in structural biology

authors： Klepeis JL,Lindorff-Larsen K,Dror RO,Shaw DE

更新日期：2009-04-01 00:00:00

abstract：:Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and com...

journal_title：Current opinion in structural biology

authors： Ellis RJ

更新日期：2001-02-01 00:00:00

abstract：:Magic-angle spinning solid-state NMR experiments are well suited to investigating the structures and mechanisms of important proteins that are inaccessible to X-ray crystallography and solution NMR spectroscopy, including membrane proteins and disease-related protein aggregates. Good progress has been made in the deve...

journal_title：Current opinion in structural biology

authors： Thompson LK

更新日期：2002-10-01 00:00:00

abstract：:ATP-binding cassette (ABC)-type ATPases are chemo-mechanical engines for diverse biological pathways. ABC ATPase domains act not only in ABC transporters but also in DNA mismatch, nucleotide excision and double-strand break repair enzymes, as well as in chromosome segregation. Atomic-resolution crystal structures sugg...

journal_title：Current opinion in structural biology

authors： Hopfner KP,Tainer JA

更新日期：2003-04-01 00:00:00

abstract：:ATP-dependent initiation factors help process replication origins and coordinate replisome assembly to control the onset of DNA synthesis. Although the specific properties and regulatory mechanisms of initiator proteins can vary greatly between different organisms, certain nucleotide-binding elements and assembly patt...

journal_title：Current opinion in structural biology

authors： Duderstadt KE,Berger JM

更新日期：2013-02-01 00:00:00

abstract：:Alternatively spliced protein segments tend to be intrinsically disordered and contain linear interaction motifs and/or post-translational modification sites. An emerging concept is that differential inclusion of such disordered segments can mediate new protein interactions, and hence change the context in which the b...

journal_title：Current opinion in structural biology

authors： Buljan M,Chalancon G,Dunker AK,Bateman A,Balaji S,Fuxreiter M,Babu MM

更新日期：2013-06-01 00:00:00

abstract：:Structural modeling of a cell is an evolving strategic direction in computational structural biology. It takes advantage of new powerful modeling techniques, deeper understanding of fundamental principles of molecular structure and assembly, and rapid growth of the amount of structural data generated by experimental t...

journal_title：Current opinion in structural biology

authors： Vakser IA,Deeds EJ

更新日期：2019-04-01 00:00:00

abstract：:Although intermediates have long been recognised as fascinating species that form during the folding of large proteins, the role that intermediates play in the folding of small, single-domain proteins has been widely debated. Recent discoveries using new, sensitive methods of detection and studies combining simulation...

journal_title：Current opinion in structural biology

authors： Brockwell DJ,Radford SE

更新日期：2007-02-01 00:00:00

abstract：:Phage display is the longest-standing platform among molecular display technologies. Recent developments have extended its utility to proteins that were previously recalcitrant to phage display. The technique has played a dominant role in forming the field of synthetic binding protein engineering, where novel interfac...

journal_title：Current opinion in structural biology

authors： Sidhu SS,Koide S

更新日期：2007-08-01 00:00:00

abstract：:Specialized metabolic enzymes and metabolite diversity evolve through a variety of mechanisms including promiscuity, changes in substrate specificity, modifications of gene expression and gene duplication. For example, gene duplication and substrate binding site changes led to the evolution of the glucosinolate biosyn...

journal_title：Current opinion in structural biology

authors： Leong BJ,Last RL

更新日期：2017-12-01 00:00:00

abstract：:The anaphase-promoting complex/cyclosome (APC/C) is a large multi-subunit complex that functions as a RING domain E3 ubiquitin ligase to regulate transitions through the cell cycle, achieved by controlling the defined ubiquitin-dependent degradation of specific cell cycle regulators. APC/C activity and substrate selec...

journal_title：Current opinion in structural biology

authors： Barford D

更新日期：2020-04-01 00:00:00

abstract：:Force spectroscopy has developed into an indispensable tool for studying folding and binding of proteins on a single molecule level in real time. Design of the pulling geometry allows tuning the reaction coordinate in a very precise manner. Many recent experiments have taken advantage of this possibility and have prov...

journal_title：Current opinion in structural biology

authors： Zoldák G,Rief M

更新日期：2013-02-01 00:00:00

abstract：:Current knowledge about the determinants of beta-sheet formation has been notably improved by the structural and kinetic analysis of model peptides, by mutagenesis experiments in proteins and by the statistical analysis of the protein structure database (Protein Data Bank; PDB). In the past year, several peptides comp...

journal_title：Current opinion in structural biology

authors： Lacroix E,Kortemme T,Lopez de la Paz M,Serrano L

更新日期：1999-08-01 00:00:00

abstract：:The 5' m7GpppN cap plays an essential role in the life cycle of eukaryotic mRNA and is required for efficient pre-mRNA splicing, export, stability and translation. Nascent pre-mRNA is capped through a series of three enzymatic steps that result in a 5' N7-methyl guanosine linked by an inverted 5'-5' triphosphate bridg...

journal_title：Current opinion in structural biology

authors： Gu M,Lima CD

更新日期：2005-02-01 00:00:00

abstract：:The molecular bases of thermal and cold stability and adaptation, which allow proteins to remain folded and functional in the temperature ranges in which their host organisms live and grow, are still only partially elucidated. Indeed, both experimental and computational studies fail to yield a fully precise and global...

journal_title：Current opinion in structural biology

authors： Pucci F,Rooman M

更新日期：2017-02-01 00:00:00

abstract：:Over the past two decades, mass spectrometry (MS) has emerged as a bone fide approach for structural biology. MS can inform on all levels of protein organization, and enables quantitative assessments of their intrinsic dynamics. The key advantages of MS are that it is a sensitive, high-resolution separation technique ...

journal_title：Current opinion in structural biology

authors： Benesch JL,Ruotolo BT

更新日期：2011-10-01 00:00:00