New approaches to the dynamic interpretation and prediction of NMR relaxation data from proteins.

Abstract:

:NMR relaxation experiments of isotopically labeled proteins provide a wealth of information on reorientational global and local dynamics on nanosecond and subnanosecond timescales for folded and nonfolded proteins in solution. Recent methodological advances in the interpretation of relaxation data have led to a better understanding of the overall tumbling behavior, the separability of internal and overall motions, and the presence of correlated dynamics between different nuclear sites, as well as to new insights into the relationship between reorientational dynamics and primary and tertiary protein structure. Some of the new methods are particularly useful when dealing with nonfolded protein states.

journal_name

Curr Opin Struct Biol

authors

Brüschweiler R

doi

10.1016/s0959-440x(03)00036-8

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

175-83

issue

2

eissn

0959-440X

issn

1879-033X

pii

S0959440X03000368

journal_volume

13

pub_type

杂志文章,评审
  • A lipid's eye view of membrane protein crystallization in mesophases.

    abstract::Lipidic mesophases have been used to produce diffraction-quality crystals of several membrane proteins. The mechanism by which the method works is a mystery. The thrust is to continue to use it whilst deciphering the underlying mechanism and solving the second 'phase problem' in membrane protein crystallography. The m...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/s0959-440x(00)00119-6

    authors: Caffrey M

    更新日期:2000-08-01 00:00:00

  • Structural view on recycling of archaeal and eukaryotic ribosomes after canonical termination and ribosome rescue.

    abstract::Ribosome recycling usually occurs after canonical termination triggered by a stop codon. Additionally, ribosomes that are stalled by aberrant mRNAs need to be recognized and subsequently recycled. In eukaryotes and archaea, the factors involved in canonical termination and ribosome rescue are structurally and function...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2012.08.002

    authors: Franckenberg S,Becker T,Beckmann R

    更新日期:2012-12-01 00:00:00

  • Mechanical unfolding of proteins: insights into biology, structure and folding.

    abstract::Since user-friendly atomic force microscopes came onto the market a few years ago, scientists have explored the response of many proteins to applied force. This field has now matured beyond the phenomenological with exciting recent developments, particularly with regards to research into biological questions. For exam...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2007.01.006

    authors: Forman JR,Clarke J

    更新日期:2007-02-01 00:00:00

  • Structural interconversions of the anaphase-promoting complex/cyclosome (APC/C) regulate cell cycle transitions.

    abstract::The anaphase-promoting complex/cyclosome (APC/C) is a large multi-subunit complex that functions as a RING domain E3 ubiquitin ligase to regulate transitions through the cell cycle, achieved by controlling the defined ubiquitin-dependent degradation of specific cell cycle regulators. APC/C activity and substrate selec...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2019.11.010

    authors: Barford D

    更新日期:2020-04-01 00:00:00

  • Designing switchable enzymes.

    abstract::The modulation of enzyme function is a key regulatory feature of biological systems. The ability to engineer synthetic enzymes that can be controlled by any arbitrary signal would enable a wide array of sensing applications and therapeutics and provide us with powerful tools for the basic study of biology. Here severa...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2009.04.007

    authors: Ostermeier M

    更新日期:2009-08-01 00:00:00

  • High-symmetry protein assemblies: patterns and emerging applications.

    abstract::The accelerated elucidation of three-dimensional structures of protein complexes, both natural and designed, is providing new examples of large supramolecular assemblies with intriguing shapes. Those with high symmetry - based on the geometries of the Platonic solids - are particularly notable as their innately closed...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2019.03.008

    authors: Cannon KA,Ochoa JM,Yeates TO

    更新日期:2019-04-01 00:00:00

  • Charge transport in DNA.

    abstract::The base pair stack within double helical DNA provides an effective medium for charge transport. The DNA pi-stack mediates oxidative DNA damage over long molecular distances in a reaction that is exquisitely sensitive to the sequence-dependent conformation and dynamics of DNA. A mixture of tunneling and hopping mechan...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/s0959-440x(02)00327-5

    authors: Boon EM,Barton JK

    更新日期:2002-06-01 00:00:00

  • The ribosome as a versatile catalyst: reactions at the peptidyl transferase center.

    abstract::In all contemporary organisms, the active site of the ribosome--the peptidyl transferase center--catalyzes two distinct reactions, peptide bond formation between peptidyl-tRNA and aminoacyl-tRNA as well as the hydrolysis of peptidyl-tRNA with the help of a release factor. However, when provided with appropriate substr...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2013.04.012

    authors: Rodnina MV

    更新日期:2013-08-01 00:00:00

  • Structural investigation of the proton-coupled secondary transporters.

    abstract::Transmembrane proton gradients, known as Proton Motive Force (PMF), serve important physiological functions. In addition to driving ATP synthesis, PMF is harnessed by a large variety of secondary transporters to achieve 'uphill' translocation of specific substrates across the membrane. Proton-coupled secondary transpo...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2013.04.011

    authors: Yan N

    更新日期:2013-08-01 00:00:00

  • Halogenases: structures and functions.

    abstract::Over 5000 halogenated natural products have been reported so far, many of these arising from the marine environment. The introduction of a halogen into a molecule can significantly impact its bioavailability and bioactivity. More recently enzymatic halogenation has been used to enable late stage functionalisation thro...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2020.05.012

    authors: Ludewig H,Molyneux S,Ferrinho S,Guo K,Lynch R,Gkotsi DS,Goss RJ

    更新日期:2020-12-01 00:00:00

  • Predicting protein function from sequence and structural data.

    abstract::When a protein's function cannot be experimentally determined, it can often be inferred from sequence similarity. Should this process fail, analysis of the protein structure can provide functional clues or confirm tentative functional assignments inferred from the sequence. Many structure-based approaches exist (e.g. ...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2005.04.003

    authors: Watson JD,Laskowski RA,Thornton JM

    更新日期:2005-06-01 00:00:00

  • Working in concert: the metalloaminopeptidases from Plasmodium falciparum.

    abstract::Malaria remains the world's most prevalent human parasitic disease. Because of the rapid spread of drug resistance in parasites, there is an urgent need to identify diverse new drug targets. One group of proteases that are emerging as targets for novel antimalarials are the metalloaminopeptidases. These enzymes cataly...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2013.07.015

    authors: McGowan S

    更新日期:2013-12-01 00:00:00

  • Characterizing transition states in protein folding: an essential step in the puzzle.

    abstract::Transition states in protein folding are being analyzed experimentally and by computer simulation to reveal the sequence of events in the folding and unfolding of proteins at high resolution. Results of this analysis have enabled the reconstruction of folding pathways in vitro and open the prospect of analyzing pathwa...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/0959-440x(95)80012-p

    authors: Fersht AR

    更新日期:1995-02-01 00:00:00

  • Beta propellers: structural rigidity and functional diversity.

    abstract::Recently solved structures and proposed models have helped to reveal the structural characteristics of the beta-propeller fold, as well as the features that contribute to its high rigidity and stability. Possible strategies for identifying beta-propeller proteins in newly characterised sequences are helping to overcom...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/s0959-440x(99)00035-4

    authors: Fülöp V,Jones DT

    更新日期:1999-12-01 00:00:00

  • Evolution of protein structures and interactions from the perspective of residue contact networks.

    abstract::Here we review mechanisms of protein evolution leading to structural changes in protein complexes. These mechanisms include mutations directly within protein interfaces, as well as the effects of mutations that propagate from distant regions of the protein. We also discuss the constraints protein complex structures im...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2013.07.004

    authors: Zhang X,Perica T,Teichmann SA

    更新日期:2013-12-01 00:00:00

  • A story with a good ending: tRNA 3'-end maturation by CCA-adding enzymes.

    abstract::CCA-adding enzymes (tRNA nucleotidyltransferases) are responsible for the maturation or repair of the functional 3' end of tRNAs. These enzymes are remarkable because they polymerize the essential nucleotides CCA onto the 3' terminus of tRNA precursors without using a nucleic acid template. Recent crystal structures, ...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2005.12.001

    authors: Xiong Y,Steitz TA

    更新日期:2006-02-01 00:00:00

  • Protein-biotin interactions.

    abstract::The three-dimensional structures of several biotin-binding proteins are now known, giving insights into the molecular architecture of the binding sites for biotin. In combination with biochemical and computational approaches, these structural insights provide the basis for our present understanding of biotin-protein i...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/s0959-440x(96)80010-8

    authors: Lindqvist Y,Schneider G

    更新日期:1996-12-01 00:00:00

  • Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.

    abstract::Small-angle X-ray scattering (SAXS) has emerged as an enabling integrative technique for comprehensive analyses of macromolecular structures and interactions in solution. Over the past two decades, SAXS has become a mainstay of the structural biologist's toolbox, supplying multiplexed measurements of molecular shape a...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2019.04.004

    authors: Brosey CA,Tainer JA

    更新日期:2019-10-01 00:00:00

  • Regulation of protein kinases by lipids.

    abstract::Membranes are sites of intense signaling activity within the cell, serving as dynamic scaffolds for the recruitment of signaling molecules and their substrates. The specific and reversible localization of these signaling molecules to membranes is critical for the appropriate activation of downstream signaling pathways...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2011.07.006

    authors: Leonard TA,Hurley JH

    更新日期:2011-12-01 00:00:00

  • Racing to build a wall: glycoconjugate assembly in Gram-positive and Gram-negative bacteria.

    abstract::The last two years have seen major advances in understanding the structural basis of bacterial cell envelope glycoconjugate biosynthesis, including capsules, lipopolysaccharide, teichoic acid, cellulose, and peptidoglycan. The recent crystal and cryo-electron microscopy structures of proteins involved in the initial g...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2020.11.013

    authors: Liston SD,Willis LM

    更新日期:2021-01-08 00:00:00

  • Phage display for engineering and analyzing protein interaction interfaces.

    abstract::Phage display is the longest-standing platform among molecular display technologies. Recent developments have extended its utility to proteins that were previously recalcitrant to phage display. The technique has played a dominant role in forming the field of synthetic binding protein engineering, where novel interfac...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2007.08.007

    authors: Sidhu SS,Koide S

    更新日期:2007-08-01 00:00:00

  • Promiscuity, impersonation and accommodation: evolution of plant specialized metabolism.

    abstract::Specialized metabolic enzymes and metabolite diversity evolve through a variety of mechanisms including promiscuity, changes in substrate specificity, modifications of gene expression and gene duplication. For example, gene duplication and substrate binding site changes led to the evolution of the glucosinolate biosyn...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2017.07.005

    authors: Leong BJ,Last RL

    更新日期:2017-12-01 00:00:00

  • Structural biology of telomerase and its interaction at telomeres.

    abstract::Telomerase is an RNP that synthesizes the 3' ends of linear chromosomes and is an important regulator of telomere length. It contains a single long non-coding telomerase RNA (TER), telomerase reverse transcriptase (TERT), and other proteins that vary among organisms. Recent progress in structural biology of telomerase...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2017.06.010

    authors: Wang Y,Feigon J

    更新日期:2017-12-01 00:00:00

  • Macromolecular crowding: an important but neglected aspect of the intracellular environment.

    abstract::Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and com...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/s0959-440x(00)00172-x

    authors: Ellis RJ

    更新日期:2001-02-01 00:00:00

  • Molecular dynamics simulations of large macromolecular complexes.

    abstract::Connecting dynamics to structural data from diverse experimental sources, molecular dynamics simulations permit the exploration of biological phenomena in unparalleled detail. Advances in simulations are moving the atomic resolution descriptions of biological systems into the million-to-billion atom regime, in which n...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2015.03.007

    authors: Perilla JR,Goh BC,Cassidy CK,Liu B,Bernardi RC,Rudack T,Yu H,Wu Z,Schulten K

    更新日期:2015-04-01 00:00:00

  • A prokaryotic perspective on pentameric ligand-gated ion channel structure.

    abstract::The X-ray structures of two prokaryotic pentameric ligand-gated ion channels have recently provided detailed insight into this important family of neurotransmitter receptors. These prokaryotic homologs share the overall architecture of their eukaryotic counterparts with conservation in functionally important residues....

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2009.07.006

    authors: Hilf RJ,Dutzler R

    更新日期:2009-08-01 00:00:00

  • Determinants of affinity and specificity in RNA-binding proteins.

    abstract::Emerging data suggest that the mechanisms by which RNA-binding proteins (RBPs) interact with RNA and the rules governing specificity might be substantially more complex than those underlying their DNA-binding counterparts. Even our knowledge of what constitutes the RNA-bound proteome is contentious; recent studies sug...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2016.05.005

    authors: Helder S,Blythe AJ,Bond CS,Mackay JP

    更新日期:2016-06-01 00:00:00

  • Dynamics in multi-domain protein recognition of RNA.

    abstract::Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combinati...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2012.03.013

    authors: Mackereth CD,Sattler M

    更新日期:2012-06-01 00:00:00

  • Polypeptide GalNAc-Ts: from redundancy to specificity.

    abstract::Mucin-type O-glycosylation is a post-translational modification (PTM) that is predicted to occur in more than the 80% of the proteins that pass through the Golgi apparatus. This PTM is initiated by a family of polypeptide GalNAc-transferases (GalNAc-Ts) that modify Ser and Thr residues of proteins through the addition...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2018.12.007

    authors: de Las Rivas M,Lira-Navarrete E,Gerken TA,Hurtado-Guerrero R

    更新日期:2019-06-01 00:00:00

  • Structural insights into ligand recognition and regulation of nucleic acid-sensing Toll-like receptors.

    abstract::Toll-like receptors (TLRs) activate the innate immune system in response to invading pathogens. Although nucleic acids are one of the principal TLR ligands, they are not inherently pathogen-specific and, thus, carry the risk of triggering autoimmunity. There are multiple unique regulatory mechanisms aimed at preventin...

    journal_title:Current opinion in structural biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.sbi.2017.05.010

    authors: Shimizu T

    更新日期:2017-12-01 00:00:00