Abstract:
:Covalent modifications of histone proteins regulate a wide variety of cellular processes. Methylation of histone H3K79 and H3K4 is associated with active transcription and is catalyzed by Dot1L and Set1, respectively. Both Dot1L and Set1 are activated by prior ubiquitination of histone H2B on K120 in a process termed 'histone crosstalk'. Recent structures of Dot1L bound to a ubiquitinated nucleosome revealed how Dot1L is activated by ubiquitin and how Dot1L distorts the nucleosome to access its substrate. Structures of Dot1L-interacting proteins have provided insight into how Dot1L is recruited to sites of active transcription. Cryo-EM and crystallographic studies of the complex of proteins associated with Set1 (COMPASS), uncovered the architecture of COMPASS and how Set1 is activated upon complex assembly.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Worden EJ,Wolberger Cdoi
10.1016/j.sbi.2019.05.009subject
Has Abstractpub_date
2019-12-01 00:00:00pages
98-106eissn
0959-440Xissn
1879-033Xpii
S0959-440X(19)30024-7journal_volume
59pub_type
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