Intermediates: ubiquitous species on folding energy landscapes?

abstract：:Historically it has been virtually impossible to experimentally determine the contribution of residual protein entropy to fundamental protein activities such as the binding of ligands. Recent progress has illuminated the possibility of employing NMR relaxation methods to quantitatively determine the role of changes in...

journal_title：Current opinion in structural biology

authors： Wand AJ

更新日期：2013-02-01 00:00:00

abstract：:The recent and growing evidence that the efficacy of a drug can be correlated to target binding kinetics has seeded the development of a multitude of novel methods aimed at computing rate constants for receptor-ligand binding processes, as well as gaining an understanding of the binding and unbinding pathways and the ...

journal_title：Current opinion in structural biology

authors： Bruce NJ,Ganotra GK,Kokh DB,Sadiq SK,Wade RC

更新日期：2018-04-01 00:00:00

abstract：:Crystallographic studies of a number of aminoacyl-tRNA synthetases and their complexes with ATP, amino acid and cognate tRNA are leading to an increasingly detailed picture of how these sophisticated enzymes function. Within the two distinct structural classes of ten synthetases, many common features are apparent, alt...

journal_title：Current opinion in structural biology

authors： Cusack S

更新日期：1997-12-01 00:00:00

abstract：:The polycomb repressive complex 2 (PRC2) is a conserved multiprotein, repressive chromatin complex essential for development and maintenance of eukaryotic cellular identity. PRC2 comprises a trimeric core of SUZ12, EED and EZH1/2, which together with RBBP4/7 is sufficient to catalyse mono-methylation, di-methylation a...

journal_title：Current opinion in structural biology

authors： Glancy E,Ciferri C,Bracken AP

更新日期：2020-11-21 00:00:00

abstract：:Maintenance of telomeres by the enzyme telomerase is essential for genomic stability and cell viability in ciliates, vertebrates and yeast. The minimal components of telomerase required for catalytic activity are the telomerase reverse transcriptase (TERT) protein and the template-containing telomerase RNA (TER). Rece...

journal_title：Current opinion in structural biology

authors： Theimer CA,Feigon J

更新日期：2006-06-01 00:00:00

abstract：:Polyketide synthases (PKSs) form a large family of multifunctional proteins involved in the biosynthesis of diverse classes of natural products. Architecturally at least three different types of PKSs have been discovered in the microbial world and recent years have revealed tremendous versatility of PKSs, both in term...

journal_title：Current opinion in structural biology

authors： Gokhale RS,Sankaranarayanan R,Mohanty D

更新日期：2007-12-01 00:00:00

abstract：:O-Glycosylation is an increasingly recognized modification of intracellular proteins in all kingdoms of life, and its occurrence in protists has been investigated to understand its evolution and its roles in the virulence of unicellular pathogens. We focus here on two kinds of glycoregulation found in unicellular euka...

journal_title：Current opinion in structural biology

authors： West CM,Kim HW

更新日期：2019-06-01 00:00:00

abstract：:Rapid advances in the cloning and expression of glycosyltransferase genes, especially from bacteria, could open the way to overcoming difficulties in the mass production of oligosaccharides. The large-scale production of oligosaccharides using either glycosyltransferases isolated from engineered microorganisms or whol...

journal_title：Current opinion in structural biology

authors： Endo T,Koizumi S

更新日期：2000-10-01 00:00:00

abstract：:Understanding the structural rules that govern specific, high-affinity binding characteristic of aptamer-protein interactions is important in view of the increasing use of aptamers across many applications. From the modest number of 16 aptamer-protein structures currently available, trends are emerging. The flexible p...

journal_title：Current opinion in structural biology

authors： Gelinas AD,Davies DR,Janjic N

更新日期：2016-02-01 00:00:00

abstract：:The past twelve months have seen a renewal of interest in the therapeutic potential of members of the tumour necrosis factor receptor family and their cytokine ligands. This biomedical interest has spawned a number of structural studies, which have significantly deepened our understanding of the molecular basis for th...

journal_title：Current opinion in structural biology

authors： Jones EY

更新日期：2000-12-01 00:00:00

abstract：:The three-dimensional structures of membrane proteins are essential for understanding their functions, interactions and architectures. Their requirement for lipids has hampered structure determination by conventional approaches. With optimized samples, it is possible to apply solution NMR methods to small membrane pro...

journal_title：Current opinion in structural biology

authors： Marassi FM,Opella SJ

更新日期：1998-10-01 00:00:00

abstract：:The observed distribution of protein structures can give us important clues about the underlying evolutionary process, imposing important constraints on possible models. The availability of results from an increasing number of genome projects has made the development of these models an active area of research. Models ...

journal_title：Current opinion in structural biology

authors： Goldstein RA

更新日期：2008-04-01 00:00:00

abstract：:Connecting three dimensional structure and affinity is analogous to seeking the 'Archimedean point', a vantage point from where any observer can quantitatively perceive the subject of inquiry. Here we review current knowledge and challenges that lie ahead of us in the quest for this Archimedean point. We argue that cu...

journal_title：Current opinion in structural biology

authors： Kastritis PL,Bonvin AM

更新日期：2013-12-01 00:00:00

abstract：:Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and com...

journal_title：Current opinion in structural biology

authors： Ellis RJ

更新日期：2001-02-01 00:00:00

abstract：:Advances in the crystallization of biological macromolecules have come not only from the application of biochemical, molecular biological and immunological principles and techniques, but also from continued efforts to understand the crystallization process. Developments in crystallization methodologies, protocols, and...

journal_title：Current opinion in structural biology

authors： Gilliland GL,Ladner JE

更新日期：1996-10-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are central to many physiological processes, including the uptake of nutrients, the non-classical secretion of signaling molecules and toxins, multidrug resistance and the development of human disease. As one might expect from this spectrum of translocation events, these ubiquit...

journal_title：Current opinion in structural biology

authors： Schmitt L,Tampé R

更新日期：2002-12-01 00:00:00

abstract：:Phage display is the longest-standing platform among molecular display technologies. Recent developments have extended its utility to proteins that were previously recalcitrant to phage display. The technique has played a dominant role in forming the field of synthetic binding protein engineering, where novel interfac...

journal_title：Current opinion in structural biology

authors： Sidhu SS,Koide S

更新日期：2007-08-01 00:00:00

abstract：:Continuing investigations into protein-protein interactions have revealed their key role in regulating a wide range of cellular processes. Although efforts to modulate these interactions are more challenging and much less mature than work on conventional drug discovery pathways, significant progress has been made on s...

journal_title：Current opinion in structural biology

authors： Zhao L,Chmielewski J

更新日期：2005-02-01 00:00:00

abstract：:The past years have seen tremendous progress towards understanding how arrestins recognize phosphorylated G protein-coupled receptors (GPCRs). Two arrestin crystal structures, one of a pre-activated splice variant and one bound to a GPCR phosphopeptide, provided insights into the conformational changes upon phosphate ...

journal_title：Current opinion in structural biology

authors： Ostermaier MK,Schertler GF,Standfuss J

更新日期：2014-12-01 00:00:00

abstract：:Mass spectrometry is becoming a more and more powerful tool for investigating protein complexes. Recent developments, based on different ionization techniques, electrospray, desorption/ionization and others are contributing to the usefulness of MS to describe the organization and structure of large non-covalent assemb...

journal_title：Current opinion in structural biology

authors： Bich C,Zenobi R

更新日期：2009-10-01 00:00:00

abstract：:The last two years have seen major advances in understanding the structural basis of bacterial cell envelope glycoconjugate biosynthesis, including capsules, lipopolysaccharide, teichoic acid, cellulose, and peptidoglycan. The recent crystal and cryo-electron microscopy structures of proteins involved in the initial g...

journal_title：Current opinion in structural biology

authors： Liston SD,Willis LM

更新日期：2021-01-08 00:00:00

abstract：:Progress in structural biology has begun to reveal the precise architecture of integral membrane proteins. However, the manner in which these complex structures are achieved remains unclear. Recent developments are starting to shed light on the unfolding and folding of a small but growing number of membrane proteins. ...

journal_title：Current opinion in structural biology

authors： Booth PJ,Curnow P

更新日期：2006-08-01 00:00:00

abstract：:The past year has seen some major advances in the area of carbohydrate synthesis using chemical methods. Progress in all areas of synthetic methodology, including new protecting groups and coupling methods, has been reported. A number of complex carbohydrate structures have been prepared using known, as well as new, m...

journal_title：Current opinion in structural biology

authors： Bartolozzi A,Seeberger PH

更新日期：2001-10-01 00:00:00

abstract：:Eukaryotic mRNAs contain a 5' cap structure that protects the transcript against rapid exonucleolytic degradation. The regulation of cellular mRNA levels therefore depends on a precise control of the mRNA decapping pathways. The major mRNA decapping enzyme in eukaryotic cells is Dcp2. It is regulated by interactions w...

journal_title：Current opinion in structural biology

authors： Wurm JP,Sprangers R

更新日期：2019-12-01 00:00:00

abstract：:Prediction and modeling of RNA structures has become an indispensable tool of biological research disciplines. Currently, reliable predictions require massive input of experimental data. Structure-forming elements are conventional base pairs, as well as a rapidly increasing repertoire of novel structural motifs. New d...

journal_title：Current opinion in structural biology

authors： Schuster P,Stadler PF,Renner A

更新日期：1997-04-01 00:00:00

abstract：:Alternatively spliced protein segments tend to be intrinsically disordered and contain linear interaction motifs and/or post-translational modification sites. An emerging concept is that differential inclusion of such disordered segments can mediate new protein interactions, and hence change the context in which the b...

journal_title：Current opinion in structural biology

authors： Buljan M,Chalancon G,Dunker AK,Bateman A,Balaji S,Fuxreiter M,Babu MM

更新日期：2013-06-01 00:00:00

abstract：:Due to the contribution of drug-target binding kinetics to drug efficacy, there is a high level of interest in developing methods to predict drug-target binding kinetic parameters. During the review period, a wide range of enhanced sampling molecular dynamics simulation-based methods has been developed for computing d...

journal_title：Current opinion in structural biology

authors： Nunes-Alves A,Kokh DB,Wade RC

更新日期：2020-10-01 00:00:00

abstract：:Membranes are sites of intense signaling activity within the cell, serving as dynamic scaffolds for the recruitment of signaling molecules and their substrates. The specific and reversible localization of these signaling molecules to membranes is critical for the appropriate activation of downstream signaling pathways...

journal_title：Current opinion in structural biology

authors： Leonard TA,Hurley JH

更新日期：2011-12-01 00:00:00

abstract：:Few biological macromolecular complexes exhibit the combination of massive size and hierarchical, symmetrical architecture embodied in icosahedral viruses. X-ray crystallography, electron cryomicroscopy and small-angle X-ray scattering provide complementary approaches to studying these remarkable structures. Through a...

journal_title：Current opinion in structural biology

authors： Lee KK,Johnson JE

更新日期：2003-10-01 00:00:00

abstract：:Solid-state NMR (ssNMR) offers insight into the formation of protein complexes and ligand binding for a large range of molecular sizes and binding affinities. Recent instrumental and methodological progress has enabled novel possibilities for using multi-dimensional ssNMR to study molecular 3D structures and interacti...

journal_title：Current opinion in structural biology

authors： Baldus M

更新日期：2006-10-01 00:00:00